*3.2.1.2. Fish toxicity test procedure*

Using OECD methodologies for acute toxicity, the lethal concentrations for 50% of tested organisms were estimated. Metals' long‐term toxicities on fish were conducted using an in‐ house methodology based on the changes in some physiological indicators such as growth rate, mortality, biomass, production, food use and biochemical indicators, hepatic enzyme activity, respectively. **Table 3** presents the technical parameters of fish toxicity tests.
