**2. Localization of leptin receptor in mouse skin**

An immunohistochemical analysis of mouse skin using anti-leptin receptor antibody revealed that leptin receptor was expressed in prickle and granular cells of epidermis (**Figure 1**). These findings showed that epidermal cells are target of leptin.

**Figure 1.** Immunohistochemical localization of leptin receptor in normal mouse skin. (A) Leptin, (B) negative control. Leptin receptor was expressed in prickle and granular cells of epidermis of mouse skin.
