**2.3. Model of dorsal skin flap**

"Double H" flaps were described following Quirinia et al.'s classification: this model was modified and adopted [7, 8].

The animals (mice) underwent general anesthesia with sodium thiopental (80 mg/kg intra‐ peritoneal injection) were scrubbed with iodine povidone on their back – their back skin shaved and rinsed with physiological serum. Double H flap consists of two opposite flaps: the former pedicled from the cranial side and the latter from the caudal one, both to be incised and elevated on the dorsal skin. The central horizontal wound together with the two distal parts of the flaps acts as critical ischemia‐risk area. The flaps were sutured back with separate stitches in Nylon 4/0. The animals were divided into three groups and sacrifices were performed on Days 3, 6, and 12, respectively. As for incisional wounds, three flap segments were taken and processed the cranial and distal one for molecular essays, the central one for histology and immunocy‐ tochemistry
