**Part 2**

**Digital Holographic Microscopy** 

180 Advanced Holography – Metrology and Imaging

Stetson, K.A. & Brohinsky, W.R. (1985). Electrooptic holography, its application to hologram

White, A.G., Smith, C. P., Heckenberg, N.R., Rubinsztein-Dunlop, H., Mcduff, R. & Weiss, C.

Yamaguchi, I. & Zhang, T. (1997). Phase-shifting digital holography. *Opt. Lett.*, Vol. 23. Pp.

Yamaguchi, I., Matsumura, T. & Kato, J. (2002). Phase-shifting colour digital holography.

Yaroslavsky, L. & Eden, M. (1996). *Fundamentals of Digital Optics.* BirkhŠuser, Boston, USA. Zhang, F., Yamaguchi, I. & Yaroslavsky, L.P. (2004). Algorithm for reconstruction of digital holograms with adjustable magnification. *Opt. Lett.*, Vol. 29, pp. 1668-1670. Zito, G., Finizio, A. & De Nicola, S. (2009). Spatially resolved refractive index profiles of

O. (1991). Interferometric measurements of phase singularities in the output of a

electrically switchable computer-generated holographic gratings. *Opt. Express*, Vol.

interferometry. *App. Opt.*, Vol. 24, pp. 3631.

visible laser. *J. Mod. Opt.*, Vol. 12, pp. 2531-41.

*Optics Letters*, Vol. 27, pp. 1108-1110.

1268-1270.

17, pp. 18843-18851.

Vest, C.M. (1979). *Holographic Interferometry*. John Wiley, New York, USA.

**9** 

*Cuba* 

**Alternative Reconstruction Method** 

Holography is a method for storing and reconstructing both amplitude and phase information of a wave front. In digital holography the reconstruction process is accomplished by means of a computer (Yaroslavsky & Merzyalov, 1980) obtaining directly the phase distribution of the object wave front. Particularly with the improvement of the spatial resolution of CCD cameras and the increasing computational performance of personal computers digital holography has been widely applied in many fields such as deformation analysis (Schedin et al., 2001), object contouring (Wagner et al., 2000), microscopy (Takaki & Ohzu, 1999) and particle measurement (Murata & Yasuda, 2000). The technique of digital holography has been implemented in a configuration of an optical microscope (Schilling et al., 1997); the objective lens produces a magnified image of the object and the interference between this image and the reference beam is achieved by the integration of the microscope into one of the arms of a Mach-Zender interferometer. This

DHM is a powerful technique for real-time quantitative phase contrast imaging, since a single intensity image, called a hologram, allows the reconstruction of the phase shift induced by a specimen. This property of holograms offers phase-contrast techniques, which can then be used for quantitative 3D imaging (Palacios et al., 2005). Quantitative phase imaging is important because it allows the determination of the optical thickness profile of a transparent object with sub-wavelength accuracy (Yu et al., 2009). Through numerical processing of the hologram one can filter out parasitic interferences and the components of the image reconstruction: zero-order and twin image terms (Cuche et al., 2000) or to compensate for curvature introduced by the microscope objective (MO) (Pedrini et al., 2001),

\* Oneida Font1, Jorge Ricardo1, Guillermo Palacios1, Mikiya Muramatsu2, Diogo Soga2,

configuration is called Digital Holographic Microscopy (DHM).

Daniel Palacios3, José Valin4 and Freddy Monroy5

*4Polytechnic Institute "José A. Echeverría", Cuba 5National University of Colombia, Colombia* 

*1University of Oriente, Cuba 2University of Sao Paulo, Brasil* 

*3University of Simon Bolivar, Venezuela* 

**1. Introduction**

**and Object Analysis in Digital** 

 **Holographic Microscopy** 

Francisco Palacios et al \*

*University of Oriente* 
