**8. Conclusion and recommendation**

Toxoplasmosis is a zoonotic disease transmitted by common foods; the occurrence of cysts of *T. gondii* in meat for human consumption represents a potential health risk, especially if the meat is consumed raw or cooked at lower temperatures of 67°C. In this sense the existence of accurate and reliable diagnostic tests is essential for the detection, monitoring and control of infections in intermediate hosts and to minimize the risk of infection in humans.

For the diagnosis of infection in tissue samples from naturally infected animals intended for consumption, it is desirable to use the technique Taqman qPCR probes since it is a more sensitive alternative to the same qPCR detection when used with SYBR Green dye. The format for performance this technique (qPCR with a Taqman probe) is versatile, allowing to evaluate both few samples (in a research environment for example) and in environments where it is required to test a greater number of specimens in a short time period (i.e., specialized labora‐ tories for monitoring food safety), and increasingly, inputs for this procedure are more affordable.
