*2.2.2.3. Assessing quality and accuracy of de novo assembled transcriptome*

Because of sequencing errors and presence of repeats in the genome, it is hard to achieve a perfect assembly. Moreover, different assemblers use different heuristic approaches to assemble the transcriptome, which results in different number of identified transcripts.

Quality andaccuracy of assembledtranscriptome are assessedinseveraldifferent ways [84, 85]:

