**4.3. SNPStream (Beckman coulter)**

This method involves a single-base extension assay and tag array technology. It starts with a multiplexed SNP-specific PCR followed by a primer extension reaction using tagged primers and fluorescent-labeled nucleotide terminators, i.e., ddNTPs. The products are captured on a tag array, which is then scanned to detect the hybridized extension primers and produce calls. It allows the processing of up to three million genotypes in 384 samples at a time. This genotyping system combines solid-phase primer extension assay and universal tags for SNP genotyping. The instrument allows processing of 4,600–3,000,000 genotypes per day [167].
