**4.1. GoldenGate Genotyping Technology (GGGT)**

The Illumina GGGT is a custom-based platform that covers construction of 96-1536 SNPs assay. The method is based on BeadArray technology, which includes immobilization of genomic DNA on avidin-coated particle. A further step is annealing of two allele-specific oligonucleo‐ tides and a locus-specific oligonucleotide for each SNP, later allele-specific primer extension for generating allele-specific products followed by PCR amplification with universal primers. It is a custom-based genotyping platform that allows screening of a vast number of samples (up to 3072 SNPs) using a single multiplexed assay. Shirasawa et al. [77] utilized 1536-plex SNP genotyping in tomato, of which 1293 were genotyped successfully. Moreover, 1248 SNPs showed clear polymorphism in 663 accessions. For eggplant, Barchi et al. [73] identified >10,000 potential SNPs. Of these, 384 highest quality SNPs were used to genotype 23 diverse eggplant germplasm with respect to fruit shape and color, and observed polymorphic information content values ranged from 0.29 to 0.5 with a mean value of 0.43.
