Contents

**Preface XIII**


Chapter 14 **DNA-based Diagnosis of Uncharacterized Inherited Macrothrombocytopenias Using Next-generation Sequencing Technology with a Candidate Gene Array 387** David J. Rabbolini, Marie-Christine Morel Kopp, Sara Gabrielli, Qiang Chen, William S. Stevenson and Christopher M. Ward

Chapter 15 **Clinical Implementation of Next-generation Sequencing in the Field of Prenatal Diagnostics 409** Gwendolin Manegold-Brauer and Olav Lapaire

Chapter 7 **RNA-seq – Revealing Biological Insights in Bacteria 205**

**Genome Analysis 229**

Ramchiary

**VI** Contents

Tolstoy and Tatiana Tatusova

Livia Stavolone and Ismail Rabbi

**Sequencing 323** Hiromasa Yamauchi

Chapter 12 **Analysis of Haplotype Sequences 345**

Mariana P. Santana, Flavia F. Aburjaile, Mariana T.D. Parise,

Chapter 8 **Dealing with the Data Deluge – New Strategies in Prokaryotic**

**Section 3 Next Generation Sequencing of Agricultural Plants 245**

Chapter 9 **Reaping the Benefits of Next-generation Sequencing**

Sandeep Tiwari, Artur Silva, Vasco Azevedo and Anne Cybele Pinto

Leonid Zaslavsky, Stacy Ciufo, Boris Fedorov, Boris Kiryutin, Igor

**Technologies for Crop Improvement — Solanaceae 247** Sushil Satish Chhapekar, Rashmi Gaur, Ajay Kumar and Nirala

Chapter 10 **Perspectives on the Application of Next-generation Sequencing to the Improvement of Africa's Staple Food Crops 287** Melaku Gedil, Morag Ferguson, Gezahegn Girma, Andreas Gisel,

Chapter 11 **Hop-variety Identification Using First- and Second-generation**

Sally S. Lloyd, Edward J. Steele and Roger L. Dawkins

Chapter 13 **On Genotyping Polymorphic HLA Genes — Ambiguities and**

**Technology with a Candidate Gene Array 387**

Szilveszter Juhos, Krisztina Rigó and György Horváth

**Macrothrombocytopenias Using Next-generation Sequencing**

David J. Rabbolini, Marie-Christine Morel Kopp, Sara Gabrielli, Qiang Chen, William S. Stevenson and Christopher M. Ward

**Section 4 Next Generation Sequencing in Humanomics 343**

**Quality Measures Using NGS 369**

Chapter 14 **DNA-based Diagnosis of Uncharacterized Inherited**

Chapter 16 **Impact of Gene Annotation on RNA-seq Data Analysis 427** Shanrong Zhao and Baohong Zhang
