**Effect of Imidacloprid on Bacterial Soil Isolate** *Bacillus weihenstephanensis*

A.A. Shetti and B.B. Kaliwal

[34] Callaghan, A., Malcolm, C.A. and Hemingway, J. Biochemical studies of A and B car‐ boxylesterases from organophosphorus-resistant strains of an Italian *Culex pipiens*

[35] Botswana Ministry of Health. Botswana malaria indicator survey 2012 report. Bot‐

[37] WHO: Implementation of Indoor residual Spraying of Insecticides for Malaria Con‐

[39] Chandre, F., Darrier, F., Manga, L., Akogbeto, M. Faye, O., Mouchet, J. and Guillet, P. Status of pyrethroid resistance in *Anopheles gambiae* sensu lato. Bulletin of World

[40] Coetzee, M. Malaria and dengue vector biology and control in Southern and Eastern Africa. In: Knols, B.G.J. and Louis, C, editors. Bridging lab and field research for ge‐ netic control of disease vectors. Wageningen UR Frontis Series. No. 11 pp101–109. [41] Legwaila, M.M., Munthali, D.C., Kwerepe, B.C. and Obopile, M. Efficacy of *Bacillus thuringiensis* (var. kurstaki) against diamondback moth (*Plutella xylostella* L.) eggs and larvae on cabbage under semi-controlled greenhouse conditions. International

[42] Legwaila, M.M., Munthali, D.C., Obopile, M. and Kwerepe, B.C. Effectiveness of spi‐ nosad against diamondback moth (*Plutella xylostella* L.) eggs and larvae on cabbage under Botswana conditions. International Journal of Insect Science. 2014: 6, 15–21. [43] Hertleina, M.B., Thompsona, G.D., Subramanyamb, B. and Athanassiouc, C.G. Spino‐ sad: a new natural product for stored grain protection. Journal of Stored Products

[44] Munthali, D.C. Evaluation of cabbage varieties for resistance to the cabbage aphid.

mosquito. Pesticide Biochemistry and Physiology. 1991;41:98–206.

[36] Botswana Ministry of Health. Botswana Malaria Strategic Plan 2006–2011.

[38] Botswana Ministry of Health. Guidelines for Malaria Vector Control. 2007.

swana National Malaria Programme. 2012;

274 Insecticides Resistance

Health Organisation. 1999;77 (3):230–234.

Journal of Insect Science. 2015. 7. 39–45

Research. 2011. Vol.47 issue 3, 131–146

African Entomology. 2009. 17 (1): 1–7

trol in the WHO African Region Report. 2007. 11–13.

Additional information is available at the end of the chapter

http://dx.doi.org/10.5772/61503

#### **Abstract**

Imidacloprid is a chloronicotinyl insecticide used widely to control biting and sucking in‐ sects. The over accumulation of this pesticide in environment requires higher awareness about this pesticide. Present investigation was carried out to analyze the effect of imida‐ cloprid on antioxidant enzymes such as superoxide dismutase, catalase and peroxidase in soil isolate *Bacillus weihenstephanensis* isolated after laboratory and field studies on the toxic effect of imidacloprid. Further, the genes for the three enzymes involved in the anti‐ oxidant defense process in soil isolate *Bacillus weihenstephanensis* were sequenced and identified. Study on the effect of 10–7 to 10–3 molar concentrations of imidacloprid for a period of 24, 48, 72 and 96 h on three antioxidant enzymes superoxide dismutase, catalase and peroxidase in *Bacillus weihenstephanensis* showed that there was an increase in the ac‐ tivity of all the three antioxidant enzymes. The enzyme activity increased with an in‐ crease in the concentration of insecticide proving that the inhibitory effect is dosedependent. Further, sequencing revealed that Fe/MnSOD (sod A), hydroxyperoxidase HP(II) (Kat E) and glutathione peroxidase genes were expressed in response to stress in‐ duced by imidacloprid treatment in *Bacillus weihenstephanensis*. The present investigation indicates that imidacloprid induces the expression of antioxidant enzymes in the soil iso‐ late *Bacillus weihenstephanensis*. The synthesis of antioxidant enzymes may be helping *Ba‐ cillus weihenstephanensis* in resisting the toxic effects of imidacloprid.

**Keywords:** Imidacloprid, *Bacillus weihenstephanensis*, Antioxidant enzymes, Genomics
