**Evaluation of TMV Lesion Formation and Timing of Signal Transduction during Induction of Systemic Acquired Resistance (SAR) in Tobacco with a Computer-Assisted Method**

Zoltán Á. Nagy, György Kátay, Gábor Gullner and Attila L. Ádám

Additional information is available at the end of the chapter

http://dx.doi.org/10.5772/61828

#### **Abstract**

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*Nicotiana tabacum* L. cv. Xanthi nc plants were inoculated with tobacco mosaic virus (TMV) in order to develop a method for evaluation of lesion size and its distribution characteristics during the induction of systemic acquired resistance (SAR). All necrotic le‐ sions were scored with an image analysis software and subjected to statistical analysis. The diminished lesion size and its right-skewed, non-normal distribution seem to be an important feature of SAR response. The results showed that the degree of induced resist‐ ance differs according to the position of the leaf on the plant's shoot. In order to detect the timing of signal transduction from TMV infected leaves to distant ones, the infected leaves were removed from the tobacco plants at different time intervals. When the infect‐ ed leaves were removed after 4 days, the SAR was always induced on the distant leaves indicating complete signal transduction within 4 days.

**Keywords:** Lesion size and distribution, signal transduction, SAR, TMV, tobacco
