**5. Adaptations to hybridization in Brazilian conditions**

The method of artificial hybridization described by Rachie et al. [69], as well as Zary and Miller Jr [70], was adjusted to conditions of infrastructure from Núcleo de Pesquisa Vegetal Básica e Aplicada (NPVBA) of the Universidade Federal Rural da Amazônia (UFRA) and climatic conditions of Northern Brazil (Figure 3), which used flower pollen collected in the morning (between 06:00 and 08:00 h) and stored in the refrigerator until use.

In the evening, the flower buds were emasculated and pollinated (16:30 and 17:30 h) (Figure 3). This method provided a higher percentage of successful pollination. This result is in agreement with the Zary and Miller Jr [70] method, described by Teófilo et al. [71], who reported that the success of this method could be because the surface of the emasculated flowers are more receptive to pollen grains in the late afternoon, since, in this period, the temperature and moisture conditions are more appropriate.

**Figure 3.** Greenish flowers with flower from left in pre-anthesis stage (A); cut of standard in medium region (B); re‐ moval of the standard (C); removal of the anthers (D); application of pollen viable (E); successful pollination and pod formation (F).
