**An Introduction to Actinobacteria**

Ranjani Anandan, Dhanasekaran Dharumadurai and Gopinath Ponnusamy Manogaran

Additional information is available at the end of the chapter

http://dx.doi.org/10.5772/62329

#### **Abstract**

Actinobacteria, which share the characteristics of both bacteria and fungi, are widely dis‐ tributed in both terrestrial and aquatic ecosystems, mainly in soil, where they play an es‐ sential role in recycling refractory biomaterials by decomposing complex mixtures of polymers in dead plants and animals and fungal materials. They are considered as the bi‐ otechnologically valuable bacteria that are exploited for its secondary metabolite produc‐ tion. Approximately, 10,000 bioactive metabolites are produced by Actinobacteria, which is 45% of all bioactive microbial metabolites discovered. Especially *Streptomyces* species produce industrially important microorganisms as they are a rich source of several useful bioactive natural products with potential applications. Though it has various applica‐ tions, some Actinobacteria have its own negative effect against plants, animals, and hu‐ mans. On this context, this chapter summarizes the general characteristics of Actinobacteria, its habitat, systematic classification, various biotechnological applications, and negative impact on plants and animals.

**Keywords:** Actinobacteria, Characteristics, Habitat, Types, Secondary metabolites, Appli‐ cations, Pathogens

### **1. Introduction**

Actinobacteria are a group of Gram-positive bacteria with high guanine and cytosine content in their DNA, which can be terrestrial or aquatic. Though they are unicellular like bacteria, they do not have distinct cell wall, but they produce a mycelium that is nonseptate and more slender. Actinobacteria include some of the most common soil, freshwater, and marine type, playing an important role in decomposition of organic materials, such as cellulose and chitin, thereby playing a vital part in organic matter turnover and carbon cycle, replenishing the supply of nutrients in the soil, and is an important part of humus formation. Actinobacterial

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colonies show powdery consistency and stick firmly to agar surface, producing hyphae and conidia/sporangia-like fungi in culture media.

Actinobacteria produce a variety of secondary metabolites with high pharmacological and commercial interest. With the discovery of actinomycin, a number of antibiotics have been discovered from Actinobacteria, especially from the genus *Streptomyces*. They are widely distributed in soil with high sensitivity to acid and low pH. Actinobacteria have a number of important functions, including degradation/decomposition of all sorts of organic substances such as cellulose, polysaccharides, protein fats, organic acids, and so on. They are also responsible for subsequent decomposition of humus (resistant material) in soil and for the earthy smell of freshly ploughed soils, producing a number of antibiotics like streptomycin, terramycin, aureomycin, and so on. This chapter gives an overview about the types of Actinobacteria, their habitat, systematic classification and various biotechnological applica‐ tions, and their ill effects on plants and animals.

### **2. Habitat of Actinobacteria**

#### **2.1. Terrestrial environment**

Soil remains the most important habitat for Actinobacteria with streptomycetes existing as a major component of its population. According to numerous reports, *Streptomyces* was encoun‐ tered to be the most abundant genus isolated in each of the study. Terrestrial Actinobacteria have various interesting antimicrobial potentials. Oskay et al [1] isolated Actinobacteria that had capability of producing novel antibiotics with high antibacterial activity. In anoxic mangrove rhizosphere, Actinobacterial species such as *Streptomyces, Micromonospora*, and *Nocardioform* were found to be abundant, which is 1000 to 10000 times smaller than arable lands because of tidal influence [2]. Similarly, *Nocardia* isolated from mangrove soil produced new cytotoxic metabolites that strongly inhibited human cell lines, such as gastric adenocarcinoma [3]. Dessert soil is also considered as an extreme terrestrial environment where only certain species, especially wherein Actinobacteria, often use *Microcoleus* as a source of food. There are several reports showing the distribution of Actinobacteria in various locations, such as sandy soil (Cario, Egypt; Falmouth, MA), black alkaline soil (Karnataka, India), sandy loam soil (Keffi Metropolis, Nigeria; Presque Isle, PA), alkaline dessert soil (Wadi El Natrun, Egypt; Wadi Araba, Egypt), and subtropical dessert soil (Thar, Rajasthan), where *Streptomyces* sp. were dominant followed by the other organisms, such as *Nocardia, Nocardiopsis,* and Actinomycetes [4]. In the study of Nithya et al [5], 134 morphologically distinguished culturable Actinobac‐ teria were isolated from 10 different desert soil samples, and the isolates were found to have varying level of antibacterial activity against bacterial pathogens. Equally, Actinobacteria play a major part in rhizosphere microbial community in the turnover of recalcitrant plant organic matter, and thus the rhizosphere region is considered as one of the best habitats for isolation of these microorganisms. Priyadharsini et al [6] in her study isolated 45 morphologically distinct colonies from 12 different paddy field soils and observed their ability to inhibit the growth of *Cyperus rotundus*. The isolates include *Streptomyces* sp*., Streptoverticillium* sp*.,* *Actinomadura* sp*., Kitasatosporia* sp*., Nocardiopsis* sp*., Pseudonocardia* sp*.*, and *Kibdelosporan‐ gium* sp.

#### **2.2. Aquatic environment**

colonies show powdery consistency and stick firmly to agar surface, producing hyphae and

Actinobacteria produce a variety of secondary metabolites with high pharmacological and commercial interest. With the discovery of actinomycin, a number of antibiotics have been discovered from Actinobacteria, especially from the genus *Streptomyces*. They are widely distributed in soil with high sensitivity to acid and low pH. Actinobacteria have a number of important functions, including degradation/decomposition of all sorts of organic substances such as cellulose, polysaccharides, protein fats, organic acids, and so on. They are also responsible for subsequent decomposition of humus (resistant material) in soil and for the earthy smell of freshly ploughed soils, producing a number of antibiotics like streptomycin, terramycin, aureomycin, and so on. This chapter gives an overview about the types of Actinobacteria, their habitat, systematic classification and various biotechnological applica‐

Soil remains the most important habitat for Actinobacteria with streptomycetes existing as a major component of its population. According to numerous reports, *Streptomyces* was encoun‐ tered to be the most abundant genus isolated in each of the study. Terrestrial Actinobacteria have various interesting antimicrobial potentials. Oskay et al [1] isolated Actinobacteria that had capability of producing novel antibiotics with high antibacterial activity. In anoxic mangrove rhizosphere, Actinobacterial species such as *Streptomyces, Micromonospora*, and *Nocardioform* were found to be abundant, which is 1000 to 10000 times smaller than arable lands because of tidal influence [2]. Similarly, *Nocardia* isolated from mangrove soil produced new cytotoxic metabolites that strongly inhibited human cell lines, such as gastric adenocarcinoma [3]. Dessert soil is also considered as an extreme terrestrial environment where only certain species, especially wherein Actinobacteria, often use *Microcoleus* as a source of food. There are several reports showing the distribution of Actinobacteria in various locations, such as sandy soil (Cario, Egypt; Falmouth, MA), black alkaline soil (Karnataka, India), sandy loam soil (Keffi Metropolis, Nigeria; Presque Isle, PA), alkaline dessert soil (Wadi El Natrun, Egypt; Wadi Araba, Egypt), and subtropical dessert soil (Thar, Rajasthan), where *Streptomyces* sp. were dominant followed by the other organisms, such as *Nocardia, Nocardiopsis,* and Actinomycetes [4]. In the study of Nithya et al [5], 134 morphologically distinguished culturable Actinobac‐ teria were isolated from 10 different desert soil samples, and the isolates were found to have varying level of antibacterial activity against bacterial pathogens. Equally, Actinobacteria play a major part in rhizosphere microbial community in the turnover of recalcitrant plant organic matter, and thus the rhizosphere region is considered as one of the best habitats for isolation of these microorganisms. Priyadharsini et al [6] in her study isolated 45 morphologically distinct colonies from 12 different paddy field soils and observed their ability to inhibit the growth of *Cyperus rotundus*. The isolates include *Streptomyces* sp*., Streptoverticillium* sp*.,*

conidia/sporangia-like fungi in culture media.

4 Actinobacteria - Basics and Biotechnological Applications

tions, and their ill effects on plants and animals.

**2. Habitat of Actinobacteria**

**2.1. Terrestrial environment**

Actinobacteria are widely distributed in aquatic habitats, which may sometimes be wash‐ ed in from surrounding terrestrial habitats. It is vitally important that the numbers and kinds of Actinobacteria are interpreted in the light of information on organisms, such as *Thermoac‐ tinomyces* and *Rhodococcus coprophilus*, which are known to be good indicators of the terrestrial component of Actinobacterial propagules in water and sediments. The resistant endo‐ spores of *Thermoactinomyces* are produced in self-heating composts, overheated fodders, and surface soil, but they can be washed into aquatic habitats where they are deposited in muds and sediments. It has been assumed that these thermophiles are unable to grow at ambi‐ ent temperatures in most aquatic habitats. Similarly, the resting coccal stage of *R. coprophi‐ lus* passes into freshwater and marine habitats, where it can survive but probably does not grow.

#### *2.2.1. Freshwater*

Cross [7] in his study evidenced that Actinobacteria can readily be isolated from freshwa‐ ter sites. Some of the major type of Actinobacteria dwelling in freshwater include *Actino‐ planes, Micromonospora, Rhodococcus, Streptomyces*, and the endospore-forming *Thermoactinomyces*. *Actinoplanes* are commonly found in soils, rivers, and lakes, and the spore vesicles of these organisms have the ability to withstand prolonged desiccation, but they release their motile spores for dispersal when rehydrated [8]. The zoospores are motile by means of a tuft of flagella exhibiting chemotaxis and require an exogenous energy source. *Micromonospora* are also considered to be a common freshwater Actinobacteria and found to be indigenous to such habitats where they turnover cellulose, chitin, and lignin. Numer‐ ous reports confirmed the presence of *Micromonospora* in streams, rivers, and river sedi‐ ments and considered them to be an integral part of the aquatic microflora. Johnston & Cross [9] found that streptomycetes failed to grow in a various lakes, notably in the deeper mud layers where micromonosporae were found to be predominant, whereas another study of AI-Diwany et al [10] showed a significant correlation between micromonosporae and thermoactinomycetes isolated from the River Wharfe in West Yorkshire, where increased number of micromonosporae was found in the adjoining soil. A study revealed that *Micromonospora* spores were washed into freshwater habitats where they can remain dormant for several years [7]. Though streptomycetes spores are also continually washed into freshwater and marine habitats, there is only little evidence that they can be active in such environments. The existence of aquatic streptomycetes has been claimed, but AI-Diwany et al [10] found a high degree of correlation between counts of streptomycetes, fecal *Streptococ‐ ci,* and *Rhodococci*. Other inhabitants of freshwater include *Actinomadura madurae*, *Mycobacte‐ rium kansasii*, and *Arthrobacter, Corynebacterium,* and *Nocardia* species. The concentration of hydrophobic spores and hyphae at the water/air interface can increase the number of streptomycetes, micromonosporae, and *Rhodococci* in foam on river water. Evidence clearly shows that Actinobacteria can become active in freshwater ecosystem in the presence of suitable substrates and conditions for growth rather than specifically adapting themselves to live in such environment.

#### *2.2.2. Marine*

When comparing the Actinobacterial diversity in terrestrial environment, the greatest biodi‐ versity lies in the oceans. The marine environment is an untapped source of novel Actinobac‐ teria diversity and thus of new metabolites. Marine Actinobacteria dwelling in extremely different environment produce different types of bioactive compounds compared with terrestrial ones. Marine Actinobacteria had to adapt from extremely high pressure and anaerobic conditions at temperatures just below 0- 8 °C on the deep sea floor to high acidic conditions at temperatures of over 8- 100°C near hydrothermal vents at the mid-ocean ridges. *Rhodococcus marinonascene*, the first marine Actinomycete species to be characterized, supports the existence of marine Actinobacteria. Members of the genera *Dietzia*, *Rhodococcus, Strepto‐ myces, Salinispora, Marinophilus, Solwaraspora, Salinibacterium, Aeromicrobium marinum, William‐ sia maris*, and *Verrucosispora* have been designated as indigenous marine Actinobacteria [11– 15]. Grossart et al [16] have illustrated that Actinobacteria account for approximately 10% of the bacteria colonizing marine organic aggregates and that their antagonistic activity might be highly significant in maintaining their presence, which affects the degradation and minerali‐ zation of organic matter. The presence of indigenous marine Actinobacteria in the oceans and the distribution of marine Actinobacteria in different marine environments and habitats are confirmed by various recent researches. Innagi et al [17] isolated various marine Actinobac‐ teria, such as *Dietzia maris*, *Rhodococcus erythropolis,* and *Kocuria erythromyxa,* from a subseafloor sediment core collected at a depth of 1225 meters off Hokkaido. Jensen et al [14] isolated five new actinomycete phylotypes from marine sediments collected around the island of Guam. Similarly, Actinobacteria were also isolated from samples collected at the deepest abyss, the Challenger Deep off the Marianas, at a depth of 10,923 meters [15]. Unusual Actinobacteria, belonging to *Micrococceae, Dermatophilaceae,* and *Gordoniaceae* have been isolated from sponges. Dhanasekaran et al [18] isolated 17 Actinobacteria from soil samples belonging to the saltpan regions of Cuddalore, Parangipettai, and screened for primary antibacterial activity among which *Streptomyces* spp. and *Saccharomonospora* sp. collected showed promising antimicrobial activity against different bacteria. An antibacterial methylsubstituted β-lactam compound was isolated and characterized from *Streptomyces noursei* DPTD21 in saltpan soil of Parangipettai Porto Novo in Cuddalore district, Tamil Nadu, by Dhanasekaran et al [19]. In another study, soil and sediment samples were collected from different locations in Muthupet mangrove region, and Actinobacteria were isolated viz *Streptomyces* sp. CC17 and SM13, *Streptosporangium* sp. SH15, and *Micropolyspora* sp. S22, which showed highest larvicidal activity against *Anopheles* mosquito larvae [20]. All the above-stated Actinobacteria isolated from marine environments, such as the deep sea floor, marine inver‐ tebrates and marine snow, sea shore soil, and deep sea sediments, represent unique ecosystems that cannot be found anywhere else in the world. Equally, these isolates produce various novel metabolites, which are listed in Table 1. Even with the limited screening efforts that have been dedicated to date to marine Actinobacteria, the discovery rate of novel secondary metabolites from marine Actinobacteria has been recently exceeded terrestrial counterparts, as evident by the isolation of many new chemical entities from marine Actinobacteria.

### **3. General characteristics of Actinobacteria**

shows that Actinobacteria can become active in freshwater ecosystem in the presence of suitable substrates and conditions for growth rather than specifically adapting themselves

When comparing the Actinobacterial diversity in terrestrial environment, the greatest biodi‐ versity lies in the oceans. The marine environment is an untapped source of novel Actinobac‐ teria diversity and thus of new metabolites. Marine Actinobacteria dwelling in extremely different environment produce different types of bioactive compounds compared with terrestrial ones. Marine Actinobacteria had to adapt from extremely high pressure and anaerobic conditions at temperatures just below 0- 8 °C on the deep sea floor to high acidic conditions at temperatures of over 8- 100°C near hydrothermal vents at the mid-ocean ridges. *Rhodococcus marinonascene*, the first marine Actinomycete species to be characterized, supports the existence of marine Actinobacteria. Members of the genera *Dietzia*, *Rhodococcus, Strepto‐ myces, Salinispora, Marinophilus, Solwaraspora, Salinibacterium, Aeromicrobium marinum, William‐ sia maris*, and *Verrucosispora* have been designated as indigenous marine Actinobacteria [11– 15]. Grossart et al [16] have illustrated that Actinobacteria account for approximately 10% of the bacteria colonizing marine organic aggregates and that their antagonistic activity might be highly significant in maintaining their presence, which affects the degradation and minerali‐ zation of organic matter. The presence of indigenous marine Actinobacteria in the oceans and the distribution of marine Actinobacteria in different marine environments and habitats are confirmed by various recent researches. Innagi et al [17] isolated various marine Actinobac‐ teria, such as *Dietzia maris*, *Rhodococcus erythropolis,* and *Kocuria erythromyxa,* from a subseafloor sediment core collected at a depth of 1225 meters off Hokkaido. Jensen et al [14] isolated five new actinomycete phylotypes from marine sediments collected around the island of Guam. Similarly, Actinobacteria were also isolated from samples collected at the deepest abyss, the Challenger Deep off the Marianas, at a depth of 10,923 meters [15]. Unusual Actinobacteria, belonging to *Micrococceae, Dermatophilaceae,* and *Gordoniaceae* have been isolated from sponges. Dhanasekaran et al [18] isolated 17 Actinobacteria from soil samples belonging to the saltpan regions of Cuddalore, Parangipettai, and screened for primary antibacterial activity among which *Streptomyces* spp. and *Saccharomonospora* sp. collected showed promising antimicrobial activity against different bacteria. An antibacterial methylsubstituted β-lactam compound was isolated and characterized from *Streptomyces noursei* DPTD21 in saltpan soil of Parangipettai Porto Novo in Cuddalore district, Tamil Nadu, by Dhanasekaran et al [19]. In another study, soil and sediment samples were collected from different locations in Muthupet mangrove region, and Actinobacteria were isolated viz *Streptomyces* sp. CC17 and SM13, *Streptosporangium* sp. SH15, and *Micropolyspora* sp. S22, which showed highest larvicidal activity against *Anopheles* mosquito larvae [20]. All the above-stated Actinobacteria isolated from marine environments, such as the deep sea floor, marine inver‐ tebrates and marine snow, sea shore soil, and deep sea sediments, represent unique ecosystems that cannot be found anywhere else in the world. Equally, these isolates produce various novel metabolites, which are listed in Table 1. Even with the limited screening efforts that have been dedicated to date to marine Actinobacteria, the discovery rate of novel secondary metabolites

to live in such environment.

6 Actinobacteria - Basics and Biotechnological Applications

*2.2.2. Marine*

Actinobacteria comprises a group of branching unicellular microorganisms, most of which are aerobic-forming mycelium known as substrate and aerial. They reproduce by binary fission or by producing spores or conidia, and sporulation of Actinobacteria is through fragmentation and segmentation or conidia formation. The morphological appearance of Actinobacteria (Figure 1) is compact, often leathery, giving a conical appearance with a dry surface on culture media and are frequently covered with aerial mycelium.

**Figure 1.** Appearance of Actinobacteria isolates on Starch casein agar plate. a, c Plate view of the Actinobacterial iso‐ lates. b, d Morphology of individual colonies.

#### **3.1. Aerial mycelium**

The aerial mycelium is usually thicker than the substrate mycelium (Figure 2a). The aerial mycelium shows sufficient differentiation that a miscellaneous assortment of isolates can be segregated into a number of groups having similar morphological characteristics under fixed condition. This is designated as one of the most important criteria for the classification of the genus *Streptomyces* into species, comprising structure (cottony, velvety, or powdery), forma‐ tion of rings or concentric zones, and pigmentation.

### **3.2. Substrate mycelium**

The substrate mycelium of Actinobacteria varies in size, shape, and thickness (Figure 2b). Its color ranges from white or virtually colorless to yellow, brown, red, pink, orange, green, or black.

**Figure 2.** Abundant growth of Actinobacterial isolate on starch casein agar medium. a. Aerial mycelium. b. Reverse side of plate showing substrate mycelium.

#### **3.3. Morphological appearance**

Morphology has been an important characteristic to identify Actinobacteria isolates, which was used in the first descriptions of *Streptomyces* species (Figure 3). This is made using various standard culture media, including International *Streptomyces* Project (ISP). For nonstreptomy‐ cetes or rare Actinobacteria, strains maintained on ATCC Medium No.172 (NZ-amine glucose starch agar) (American Type Culture Collection, 1982) were used. Various morphological observations, including germination of spores, elongation and branching of vegetative mycelium, formation of aerial mycelium, color of aerial and substrate mycelium, and pigment production, have been used to identify Actinobacteria [21]. Light microscopy was used to study the formation of aerial mycelium and substrate mycelium, and scanning electron microscopy (Figure 4) was used to study the spores, the spore surface, and spore structure.

### **4. Systematics of Actinobacteria**

In the Bergey's Manual of Determinative Bacteriology, Actinobacteria are included in several sections of volume four. All Actinobacteria are included under the order Actinomycetales. The order Actinomycetales is divided into four families—Streptomycetaceae, Actinomycetaceae, Actinoplanaceae, and Mycobacteriaceae [23]. The "Bergey's Manual of Systematic Bacteriolo‐ gy—2nd edition" for Actinobacteria classification has five volumes, which contain interna‐

**Figure 3.** Type of spore-bearing structure in streptomycetes [22].

**3.2. Substrate mycelium**

8 Actinobacteria - Basics and Biotechnological Applications

side of plate showing substrate mycelium.

**3.3. Morphological appearance**

**4. Systematics of Actinobacteria**

black.

The substrate mycelium of Actinobacteria varies in size, shape, and thickness (Figure 2b). Its color ranges from white or virtually colorless to yellow, brown, red, pink, orange, green, or

**Figure 2.** Abundant growth of Actinobacterial isolate on starch casein agar medium. a. Aerial mycelium. b. Reverse

Morphology has been an important characteristic to identify Actinobacteria isolates, which was used in the first descriptions of *Streptomyces* species (Figure 3). This is made using various standard culture media, including International *Streptomyces* Project (ISP). For nonstreptomy‐ cetes or rare Actinobacteria, strains maintained on ATCC Medium No.172 (NZ-amine glucose starch agar) (American Type Culture Collection, 1982) were used. Various morphological observations, including germination of spores, elongation and branching of vegetative mycelium, formation of aerial mycelium, color of aerial and substrate mycelium, and pigment production, have been used to identify Actinobacteria [21]. Light microscopy was used to study the formation of aerial mycelium and substrate mycelium, and scanning electron microscopy (Figure 4) was used to study the spores, the spore surface, and spore structure.

In the Bergey's Manual of Determinative Bacteriology, Actinobacteria are included in several sections of volume four. All Actinobacteria are included under the order Actinomycetales. The order Actinomycetales is divided into four families—Streptomycetaceae, Actinomycetaceae, Actinoplanaceae, and Mycobacteriaceae [23]. The "Bergey's Manual of Systematic Bacteriolo‐ gy—2nd edition" for Actinobacteria classification has five volumes, which contain interna‐ tionally recognized names and descriptions of bacterial species. Classification of Actinobacteria has been rearranged as follows:


In Volume 5, the phylum Actinobacteria is divided into six classes, namely Actinobacteria, Acidimicrobiia, Coriobacteriia, Nitriliruptoria, Rubrobacteria, and Thermoleophilia. The class Actinobacteria is further divided into 16 orders that are Actinomycetales, Actinopolysporales, Bifidobacteriales, Catenulisporales, Corynebacteriales, Frankiales, Glycomycetales, Jiangel‐ 262

263

264

265

266

267

260 **Figure 3.** Type of spore-bearing structure in streptomycetes (22).

269 *Micromonospora* sp. b. *Streptosporangium* sp. c. *Saccharopolyspora* sp. d. **Figure 4.** Scanning electron photographs of various Actinobacterial isolates. a. *Micromonospora* sp. b. *Streptosporangium* sp. c. *Saccharopolyspora* sp. d. *Actinosynnema* sp. e. *S. noursei* DPTD21. f. *Streptomyces* sp. JD9.

268 **Figure 4.** Scanning electron photographs of various Actinobacterial isolates. a.

274 under the order Actinomycetales. The order Actinomycetales is divided into

lales, Kineosporiales, Micrococcales, Micromonosporales, Propionibacteriales, Pseudonocar‐ diales, Streptomycetales, Streptosporangiales, and Incertae sedis. In the order of abundance in soils, the common genera of Actinobacteria are *Streptomyces* (nearly 70%), *Nocardia*, and *Micromonospora*, although *Actinoplanes*, *Micromonospora*, and *Streptosporangium* are also generally encountered. At present, the molecular identification is based on 16S rDNA sequen‐ ces, which is most significant for Actinobacteria identification [24]. 270 *Actinosynnema* sp. e. *S. noursei* DPTD21. f. *Streptomyces* sp. JD9. 271 **1.4 Systematics of Actinobacteria** 272 In the Bergey's Manual of Determinative Bacteriology, Actinobacteria are 273 included in several sections of volume four. All Actinobacteria are included

#### **5. Types of Actinobacteria** 275 four families—Streptomycetaceae, Actinomycetaceae, Actinoplanaceae, and

#### **5.1. Thermophilic Actinobacteria** 276 Mycobacteriaceae (23). The "Bergey's Manual of Systematic Bacteriology—

Number of studies has been carried out by the researchers to confirm the existence of extremophilic and extreme tolerant soil Actinobacteria (acid tolerant and alkali tolerant, psychrotolerant and thermotolerant, and halotolerant and haloalkalitolerant or xerophilic). Mesophilic Actinobacteria can grow at an optimal temperature from 20°С to 42°С, among which thermotolerant species exist, which can survive at 50°С. Moderately thermophilic Actinobacteria have an optimum growth at 45°С–55°C [29], whereas strictly thermophilic Actinobacteria grow at 37°С–65°C with the optimum temperature at 55°С–60°C [25]. Incubation temperatures of 28°С, 37°С, and 45°C are considered optimal for isolation of soil mesophilic, thermotolerant, and moderately thermophilic Actinobacteria. *Thermoactinomy‐ ces*, which is presently excluded from the order Actinomycetales, are described as thermophil‐ ic forms depending on its phenotypic and molecular genetic characteristics, as well as among some species of *Thermomonospora, Microbispora, Saccharopolyspora, Saccharomonospora,* and *Streptomyces*. 15 277 2nd edition" for Actinobacteria classification has five volumes, which contain

### **5.2. Acidophilic Actinobacteria**

Acidophilic Actinobacteria, which are common in terrestrial habitats such as acidic forest and mine drainage soil, grow in the pH range from about 3.5 to 6.5, with optimum rates at pH 4.5 to 5.5 [26, 27]. It has been shown that acidophilic Actinobacteria consistently form two distinct aggregate taxa (namely, the neutrotolerant acidophilic and strictly acidophilic cluster groups) based on numerical phenetic data; members of the two groups share common morphological and chemotaxonomic properties [26]. Also some members of the strictly acidophilic group form a distinct taxon, such as the genus *Streptacidiphilus*, which has been assigned to the revised family Streptomycetaceae, together with the genera *Kitasatospora* and *Streptomyces*.

### **5.3. Halophilic Actinobacteria**

lales, Kineosporiales, Micrococcales, Micromonosporales, Propionibacteriales, Pseudonocar‐ diales, Streptomycetales, Streptosporangiales, and Incertae sedis. In the order of abundance in soils, the common genera of Actinobacteria are *Streptomyces* (nearly 70%), *Nocardia*, and *Micromonospora*, although *Actinoplanes*, *Micromonospora*, and *Streptosporangium* are also generally encountered. At present, the molecular identification is based on 16S rDNA sequen‐

**Figure 4.** Scanning electron photographs of various Actinobacterial isolates. a. *Micromonospora* sp. b. *Streptosporangium*

**a b c**

**d e f**

268 **Figure 4.** Scanning electron photographs of various Actinobacterial isolates. a.

269 *Micromonospora* sp. b. *Streptosporangium* sp. c. *Saccharopolyspora* sp. d.

272 In the Bergey's Manual of Determinative Bacteriology, Actinobacteria are

273 included in several sections of volume four. All Actinobacteria are included

274 under the order Actinomycetales. The order Actinomycetales is divided into

275 four families—Streptomycetaceae, Actinomycetaceae, Actinoplanaceae, and

276 Mycobacteriaceae (23). The "Bergey's Manual of Systematic Bacteriology—

277 2nd edition" for Actinobacteria classification has five volumes, which contain

Number of studies has been carried out by the researchers to confirm the existence of extremophilic and extreme tolerant soil Actinobacteria (acid tolerant and alkali tolerant, psychrotolerant and thermotolerant, and halotolerant and haloalkalitolerant or xerophilic). Mesophilic Actinobacteria can grow at an optimal temperature from 20°С to 42°С, among which thermotolerant species exist, which can survive at 50°С. Moderately thermophilic Actinobacteria have an optimum growth at 45°С–55°C [29], whereas strictly thermophilic Actinobacteria grow at 37°С–65°C with the optimum temperature at 55°С–60°C [25]. Incubation temperatures of 28°С, 37°С, and 45°C are considered optimal for isolation of soil mesophilic, thermotolerant, and moderately thermophilic Actinobacteria. *Thermoactinomy‐ ces*, which is presently excluded from the order Actinomycetales, are described as thermophil‐ ic forms depending on its phenotypic and molecular genetic characteristics, as well as among some species of *Thermomonospora, Microbispora, Saccharopolyspora, Saccharomonospora,* and

15

ces, which is most significant for Actinobacteria identification [24].

270 *Actinosynnema* sp. e. *S. noursei* DPTD21. f. *Streptomyces* sp. JD9.

sp. c. *Saccharopolyspora* sp. d. *Actinosynnema* sp. e. *S. noursei* DPTD21. f. *Streptomyces* sp. JD9.

260 **Figure 3.** Type of spore-bearing structure in streptomycetes (22).

**5. Types of Actinobacteria**

271 **1.4 Systematics of Actinobacteria**

10 Actinobacteria - Basics and Biotechnological Applications

261

262

263

264

265

266

267

**5.1. Thermophilic Actinobacteria**

*Streptomyces*.

Halophilic Actinobacteria are categorized into different types based on their growth in media containing different concentrations of salt. Extreme halophiles grow best in media containing 2.5–5.2 M salt, whereas borderline extreme halophiles grow best in media containing 1.5–4.0 M salt, moderate halophiles grow best in media containing 0.5–2.5 M salt, and finally haloto‐ lerants that do not show an absolute requirement to salt for growth but grow well up to often very high salt concentrations and tolerate 100 g/l salt (equivalent to 1.7 M NaCl) at least. Seawater, saline soils, salt lakes, brines, and alkaline saline habitats are considered as the best habitats for isolating halophilic Actinobacteria. Generally, most of the halophilic Actinobac‐ teria have been isolated from saline soils. Halophilic Actinobacteria isolated from marine environments are assigned to a few genera, including *Micromonospora, Rhodococcus,* and *Streptomyces* [28]. The other group includes *Dietzia, Salinispora, Marinophilus, Solwaraspora, Salinibacterium, Aeromicrobium, Gordonia, Microbacterium, Mycobacterium, Nocardiopsis, Pseudo‐ nocardia, Actinomadura, Saccharopolyspora, Streptosporangium, Nonomuraea, Williamsia,* and *Verrucosispora* [28–31].

### **5.4. Endophytic Actinobacteria**

Endophytic Actinobacteria are defined as those that inhabit the internal part of plants, causing apparently no visible changes to their hosts. These Actinobacteria play specific roles, for instance, protecting the host plants against insects and diseases. Endophytic Actinobacteria constitute a large part of the rhizosphere, which are also found inside plants in which the extensively studied species are from the genus *Frankia*, nitrogen-fixing bacteria of nonlegu‐ minous plants [32], and a few species of the genus *Streptomyces* that are phytopathogens. Generally, the endophytic Actinobacteria include *Streptomyces,* but the genera *Streptoverticil‐ lium*, *Nocardia*, *Micromonospora, Kitasatospora, Pseudonocardia, Microbispora*, *Kibdelosporangium, Actinopolyspora, Nocardioides, Brevibacterium, Actinomadura, Glycomyces Plantactinospora, Polymorphospora, Promicromonospora*, and *Streptosporangium* are also found in the plants, such as *Palicourea longifolia, Calycophyllum acreanum, Monstera spruceana, Croton lechleri, Cantua buxifolia, Siparuna crassifolia*, and *Eucharis cyaneosperma.*

### **5.5. Symbiotic Actinobacteria**

About 15% of the world's nitrogen is fixed naturally by the symbiotic relationships between various species of the *Frankia* belonging to the family of Actinobacteria. The plants that form symbiotic relationships with *Frankia* are called actinorhizal plants. Researchers have found over 160 plants that have Actinobacteria as their host, including alders, Russian olive, bay‐ berry, sweet fern, bitterbrush, and cliff rose. The *Frankia* have the ability to provide most or all of the host plant's nitrogen needs. Numerous *Frankia* species including *Casuarina* isolates form nitrogen-reducing (NIR) vesicles *in vitro* and in planta [33]. These nitrogen-fixing bacteria and their host plants are often pioneer species on young nitrogen-deficient and disturbed soils such as moraines, volcanic flows, and sand dunes.

### **5.6. Endosymbiontic Actinobacteria**

An endosymbiont is any organism that lives within the body or cells of another organism. Endosymbiosis process is sometimes obligate, that is, either the endosymbiont or the host cannot survive without the other. Members of the phylum Actinobacteria have been identified as abundant members of sponge-associated microbial communities. *Mycobacterium* along with *Micrococcus, Micromonospora, Microbacterium, Brevibacterium, Kocuria, Corynebacterium, Rhodo‐ coccus, Brachybacterium, Rubrobacter, Streptomyces, Dietzia, Salinispora, Actinokineospora, Gordo‐ nia, Arthrobacter, Nocardiopsis*, *and Rothia* species were found to live as endosymbionts in marine sponges *Callyspongia aff. Implexa, Aplysina aerophoba, Spheciospongia vagabunda, Hemimycale culumella, Hyrtios erecta, Dysidea tupha*, *Callyspongia* sp., *Dysidea avara*, *Amphimedon* sp., and *Negombata magnifica*. However, the Actinobacterial endosymbionts have also been reported in other group of animals, such as *Hylobates hoolock*, *Rhinopithecus roxellanae, Rhinopithecus bieti, Panthera tigris altaica, Panthera tigris tigris, Panthera tigris amoyensis, Ailurus fulgens, Cavnlvara zlrsidae, Ursus thibetanus, Cervus elaphus, Elaphurus davidianus,* and *Vicugna pacos.*

### **5.7. Gut Actinobacteria**

Though Actinobacteria are found in various diverse habitats, some are also known to form intimate associations with invertebrates and vertebrates. Symbiotic interactions are essential mainly for the survival and reproduction because they play a crucial role in nutrition, detox‐ ification of certain compounds, growth performance, and protection against pathogenic bacteria. Many studies have shown that some symbiotic Actinobacterial species, that is probiotics, control bacterial diseases in livestock, poultry, and aquaculture. They also take part in host health by converting the feedstuffs into microbial biomass and fermentation end products that can be utilized by the animal host. Tan et al [34] isolated *Streptomyces*, *Nocar‐ diopsis*, and *Oerskovia* from healthy goat feces. Similarly, Latha and Dhanasekaran [35] isolated 87 Actinobacterial cultures from different feces of goat and chicken collected from various locations in Pudukkottai and Tiruchirappalli Districts, Tamil Nadu, among which 45 isolates were selected for the screening of antibacterial activity and extracellular digestive enzyme production. The ability of the probiont *Streptomyces* sp. JD9 from gut of chicken possesses all the characteristics needed to satisfy the indigenous Actinobacterial probiont for enhanced broiler production [36].

### **6. Applications of Actinobacteria**

**5.5. Symbiotic Actinobacteria**

12 Actinobacteria - Basics and Biotechnological Applications

as moraines, volcanic flows, and sand dunes.

**5.6. Endosymbiontic Actinobacteria**

**5.7. Gut Actinobacteria**

broiler production [36].

About 15% of the world's nitrogen is fixed naturally by the symbiotic relationships between various species of the *Frankia* belonging to the family of Actinobacteria. The plants that form symbiotic relationships with *Frankia* are called actinorhizal plants. Researchers have found over 160 plants that have Actinobacteria as their host, including alders, Russian olive, bay‐ berry, sweet fern, bitterbrush, and cliff rose. The *Frankia* have the ability to provide most or all of the host plant's nitrogen needs. Numerous *Frankia* species including *Casuarina* isolates form nitrogen-reducing (NIR) vesicles *in vitro* and in planta [33]. These nitrogen-fixing bacteria and their host plants are often pioneer species on young nitrogen-deficient and disturbed soils such

An endosymbiont is any organism that lives within the body or cells of another organism. Endosymbiosis process is sometimes obligate, that is, either the endosymbiont or the host cannot survive without the other. Members of the phylum Actinobacteria have been identified as abundant members of sponge-associated microbial communities. *Mycobacterium* along with *Micrococcus, Micromonospora, Microbacterium, Brevibacterium, Kocuria, Corynebacterium, Rhodo‐ coccus, Brachybacterium, Rubrobacter, Streptomyces, Dietzia, Salinispora, Actinokineospora, Gordo‐ nia, Arthrobacter, Nocardiopsis*, *and Rothia* species were found to live as endosymbionts in marine sponges *Callyspongia aff. Implexa, Aplysina aerophoba, Spheciospongia vagabunda, Hemimycale culumella, Hyrtios erecta, Dysidea tupha*, *Callyspongia* sp., *Dysidea avara*, *Amphimedon* sp., and *Negombata magnifica*. However, the Actinobacterial endosymbionts have also been reported in other group of animals, such as *Hylobates hoolock*, *Rhinopithecus roxellanae, Rhinopithecus bieti, Panthera tigris altaica, Panthera tigris tigris, Panthera tigris amoyensis, Ailurus fulgens, Cavnlvara*

*zlrsidae, Ursus thibetanus, Cervus elaphus, Elaphurus davidianus,* and *Vicugna pacos.*

Though Actinobacteria are found in various diverse habitats, some are also known to form intimate associations with invertebrates and vertebrates. Symbiotic interactions are essential mainly for the survival and reproduction because they play a crucial role in nutrition, detox‐ ification of certain compounds, growth performance, and protection against pathogenic bacteria. Many studies have shown that some symbiotic Actinobacterial species, that is probiotics, control bacterial diseases in livestock, poultry, and aquaculture. They also take part in host health by converting the feedstuffs into microbial biomass and fermentation end products that can be utilized by the animal host. Tan et al [34] isolated *Streptomyces*, *Nocar‐ diopsis*, and *Oerskovia* from healthy goat feces. Similarly, Latha and Dhanasekaran [35] isolated 87 Actinobacterial cultures from different feces of goat and chicken collected from various locations in Pudukkottai and Tiruchirappalli Districts, Tamil Nadu, among which 45 isolates were selected for the screening of antibacterial activity and extracellular digestive enzyme production. The ability of the probiont *Streptomyces* sp. JD9 from gut of chicken possesses all the characteristics needed to satisfy the indigenous Actinobacterial probiont for enhanced Actinobacteria are well recognized for their production of primary and secondary metabolites that have important applications in various fields. They are also a promising source of wide range of important enzymes, which are produced on an industrial scale. A large fraction of antibiotics in the market is obtained from Actinobacteria. They produce enzyme inhibitors useful for cancer treatment and immunomodifiers that enhance immune response. They have the ability to degrade a wide range of hydrocarbons, pesticides, and aliphatic and aromatic compounds. They perform microbial transformations of organic compounds, a field of great commercial value. Members of many genera of Actinobacteria can be potentially used in the bioconversion of underutilized agricultural and urban wastes into high-value chemical products. Actinobacteria are also important in plant biotechnology as strains with antagonistic activity against plant pathogens are useful in biocontrol. Their metabolic potential offers a strong area for research. Here, we have a brief description about important applications of Actinobacteria (Figure 5).

**Figure 5.** Biotechnological applications of Actinobacteria.

#### **6.1. Antimicrobials**

Actinobacteria hold a significant role in producing variety of drugs that are extremely important to our health and nutrition. Recently, diseases due to multidrug-resistant patho‐ genic bacteria are sturdily increasing, and thus search for new antibiotics is effective against the multidrug-resistant pathogens. Natural products having novel structures have been observed to possess useful biological activities [37]. Nature always remains the richest and the most versatile propitious source for new antibiotics, though there is considerable progress within the fields of chemical synthesis and engineered biosynthesis of antibacterial com‐ pounds. Toxic nature of certain antibiotics led to their limited usage although thousands of antibiotics have been discovered till date. To get through this problem, search of new antibi‐ otics that are more effective and that do not have any toxic side effects is in progress. As already mentioned, one of the major healthcare problems is the antibiotic resistance. One approach to solve this problem is to search for new antibiotics with new mechanism of action. Figure 6 shows that a majority of antibiotics are derived from microorganisms, especially from the species Actinobacteria. Almost 80% of the world's antibiotics are known to be derived from Actinobacteria, mostly from the genera *Streptomyces* and *Micromonospora* [38, 39].

**Figure 6.** Antibiotics from Actinobacteria.

Particularly, *Streptomyces* species produce around 7600 compounds, many of which are secondary metabolites that are potent antibiotics, which has made streptomycetes the primary antibiotic-producing organisms exploited by the pharmaceutical industry [40, 41]. The reason behind the ability of the genus *Streptomyces* to produce commercially significant compounds remains supreme because of the extra large DNA complement of these bacteria [42]. It has been estimated that the last five decades have seen the discovery of more than 12,000 antibiotics, out of which the Actinobacteria yielded about 70% of them and the remaining 30% are products of filamentous fungi and nonActinobacteria. The antibiotics from Actinobacteria are differen‐ tiated into several major structural classes, such as aminoglycosides (e.g., streptomycin and kanamycin), ansamycins (e.g., rifampin), anthracyclines (e.g., doxorubicin), β-lactam (cepha‐ losporins), macrolides (e.g., erythromycin), and tetracycline. *Streptomyces* strains have produced many of the antibiotics known to humans, which appears that these organisms produce antibiotics to kill off potential competitors [43]. One of the first antibiotics used is streptomycin produced by *Streptomyces griseus* [44]. Indeed, different *Streptomyces* species produce about 75% of commercially and medically useful antibiotics. In the course of screening for new antibiotics, several studies are oriented toward isolation of streptomycetes from different habitats. Different conditions of nutrition and culturing may affect the ability of *Streptomyces* cultures to form antibiotics, and hence the medium constitution together with the metabolic capacity of the producing organism greatly affects antibiotic biosynthesis.

genic bacteria are sturdily increasing, and thus search for new antibiotics is effective against the multidrug-resistant pathogens. Natural products having novel structures have been observed to possess useful biological activities [37]. Nature always remains the richest and the most versatile propitious source for new antibiotics, though there is considerable progress within the fields of chemical synthesis and engineered biosynthesis of antibacterial com‐ pounds. Toxic nature of certain antibiotics led to their limited usage although thousands of antibiotics have been discovered till date. To get through this problem, search of new antibi‐ otics that are more effective and that do not have any toxic side effects is in progress. As already mentioned, one of the major healthcare problems is the antibiotic resistance. One approach to solve this problem is to search for new antibiotics with new mechanism of action. Figure 6 shows that a majority of antibiotics are derived from microorganisms, especially from the species Actinobacteria. Almost 80% of the world's antibiotics are known to be derived from

Actinobacteria, mostly from the genera *Streptomyces* and *Micromonospora* [38, 39].

Particularly, *Streptomyces* species produce around 7600 compounds, many of which are secondary metabolites that are potent antibiotics, which has made streptomycetes the primary antibiotic-producing organisms exploited by the pharmaceutical industry [40, 41]. The reason behind the ability of the genus *Streptomyces* to produce commercially significant compounds remains supreme because of the extra large DNA complement of these bacteria [42]. It has been estimated that the last five decades have seen the discovery of more than 12,000 antibiotics, out of which the Actinobacteria yielded about 70% of them and the remaining 30% are products

**Figure 6.** Antibiotics from Actinobacteria.

14 Actinobacteria - Basics and Biotechnological Applications

Antagonistic Actinobacteria produce a variety of antibiotics that vary in chemical nature, in antimicrobial action, in toxicity to animals, and in their chemotherapeutic potentialities. Some of the antibiotics that have been isolated so far from Actinobacteria are crude preparations, whereas others have been crystallized, and considerable information has been gained con‐ cerning their chemical nature, which includes lysozyme, actinomycin, micromonosporin, streptothricin, streptomycin, and mycetin. Some Actinobacteria produce more than one antibiotic substance (e.g., *S. griseus*), as well as the same antibiotic may be produced by different species of Actinobacteria (e.g, actinomycin, streptothricin). A given antibiotic may, therefore, be identical, even when produced by different Actinobacteria, as shown by its chemical composition and antibiotic spectrum. Table 1 represents the list of antibiotics produced by various Actinobacteria with excellent antimicrobial application (Table 1).



31


**Table 1.** List of antibiotics produced from Actinobacteria

#### **6.2. Enzymes**

529

530

531

532

533

537

**Antibiotic compound Application Actinobacteria**

Cyanospraside A Unknown *Solieria pacifica* Daryamides Antifungal; anticancer *Streptomyces* sp. Frigocyclinone Antibacterial *S. griseus* Glaciapyrroles Antibacterial *Streptomyces* sp.

biosynthesis

immunosuppressive

Lajollamycin Antibacterial *Streptomyces Nodosus*

biosynthesis

Mechercharmycins Anticancer *Thermoactinomyces* sp.

stranded DNA

Tetracyclines Antimicrobial *Streptomyces achromogenesStreptomyces*

Tirandamycins Antibacterial *Streptomyces* sp.

ZHD-0501 Anticancer *Actinomadura* sp. Elaiomycins B and C Antitumor *Streptomyces* sp. BK 190

Chromomycin B, A2, A3 Antitumor *Streptomyces coelicolor*

and eukaryotes

Valinomycin Ionophor, toxic for prokaryotes

N-[2-hydroxyphenyl)-2-phenazinamine

(NHP),

Marinomycins A–D Antimicrobial; anticancer *Marinispora*

Pacificanones A & B Antibacterial *S. pacifica* Piericidins Antitumor *Streptomyces* sp. Proximicins Antibacterial; anticancer *Verrucosispora* sp. Rapamycin Immunosuppressive, antifungal *S. hygroscopicus* Resistoflavin methyl ether Antibacterial; antioxidative *Streptomyces* sp. Saliniketal Cancer chemoprevention *S. arenicola* Salinispyrone Unknown *S. pacifica* Salinispyrone A & B Mild cytotoxicity *S. pacifica* Salinosporamide A Anticancer; antimalarial *Salinispora tropica* Salinosporamide B & C Cytotoxicity *S. tropica* Sesquiterpene Unknown *Streptomyces* sp. Staurosporinone Antitumor; phycotoxicity *Streptomyces* sp. Streptokordin Antitumor *Streptomyces* sp. Streptomycin Antimicrobial *S. griseus* Streptozotocin Diabetogenic *S. achromogenes*

*Streptomyces venezuelae*

*Streptomyces hygroscopicus*

*Streptomyces lincolnensis*

*Streptomyces lavendulae*

*rimosus*

*S. griseus*

Anticancer; antifungus *Nocardia dassonvillei*

Chloramphenicol Antibacterial, inhibitor of protein

Lincomycin Antibacterial, inhibitor of protein

Mitomycin C Antitumor, binds to double-

Hygromycin Antimicrobial,

16 Actinobacteria - Basics and Biotechnological Applications

A wide variety of biologically active enzymes are produced by both marine and terrestrial Actinobacteria (Figure 7; Table 2). They secrete amylases to the outside of the cells, which helps them to carry out extracellular digestion. This enzyme is of great significance in biotechnolog‐ ical applications such as food industry, fermentation, and textile to paper industries because of their ability to degrade starch [45]. Another important aspect of Actinobacteria is the produc‐ tion of cellulases, which are a collection of hydrolytic enzymes that hydrolyze the glucosidic bondsofcelluloseandrelatedcello-digosaccharidederivatives.Lipaseisproducedfromvarious Actinobacteria, bacteria, and fungi and is used in detergent industries, foodstuff, oleochemi‐ cal, diagnostic settings, and also in industries of pharmaceutical fields [46]. Many Actinobacte‐ ria have been isolated from various natural sources, as well as in plant tissues and rhizospheric soil. Biological functions of Actinobacteria mainly depend on sources from which the bacteria are isolated. Actinobacteria, particularly streptomycetes, are known to secrete multiple proteases in the culture medium [47]. Similarly, Actinobacteria have been revealed to be an excellent resource for L-asparaginase, which is produced by a range of Actinobacteria, mainly those isolated from soils, such as *S. griseus, Streptomyces karnatakensis, Streptomyces albidofla‐ vus,* and *Nocardia* sp. [48, 49]. The roots and rhizomes of several Thai medicinal plants such as lemon grass (*Cymbopogon citratus*) and ginger (*Zingiber officinale*) have long been used in Thai traditional medicine for stomach ache and asthma treatment. Rhizosphere soil of these plants may be an attractive Actinobacterial source, which has the ability to produce novel secondary metabolites.Enzymes such as catalase, chitinase, andurease are alsoproducedfromActinobac‐ teria. Interestingly, keratinase, an enzyme that degrades the poultry chicken feather, has been successfully produced from *Nocradiopsis* sp. SD5 isolated from feather waste in Tamil Nadu, India [50]. Similarly, Actinobacteria isolated from chicken and goat gut showed the presence of various enzymes such as amylase, protease, phytase, and lipase [35]. 522 which has the ability to produce novel secondary metabolites. Enzymes such as 523 catalase, chitinase, and urease are also produced from Actinobacteria. 524 Interestingly, keratinase, an enzyme that degrades the poultry chicken feather, 525 has been successfully produced from Nocradiopsis sp. SD5 isolated from 526 feather waste in Tamil Nadu, India (50). Similarly, Actinobacteria isolated from 527 chicken and goat gut showed the presence of various enzymes such as amylase, 528 protease, phytase, and lipase (35).

534 Figure 7. Different types of enzymes produced by Actinobacteria. a. Amylase. **Figure 7.** Different types of enzymes produced by Actinobacteria. a. Amylase. b. Protease. c. Lipase. The zone of inhibi‐ tion around the inoculated Actinobacteria confirms the production of particular enzyme.

Enzyme Actinobacteria Use Industry of application

Detergents Detergent

Cheese making Food

Clarification- low Brewing

535 b. Protease. c. Lipase. The zone of inhibition around the inoculated

536 Actinobacteria confirms the production of particular enzyme.

Nocardiopsis sp.,

Streptomyces pactum,

538 Table 2. Enzymes and their industrial applications

Protease Thermoactinomyces sp.,


**Table 2.** Enzymes and their industrial applications

#### **6.3. Bioherbicides**

**Enzyme Actinobacteria Use Industry of application**

cotton

fibers

Lipase *Streptomyces griseus* Removal of stains Detergent

Pectinase *Streptomyces lydicus* Clarification, mashing Beverage

Detergents Detergent Cheese making Food Clarification- low calorie beer Brewing Dehiding Leather Treatment of blood clot Medicine

Removal of stains Detergent

Textile

Baking

Baking

Textile

Paper and pulp

Starch industry

Paper and pulp

Denim finishing, softening of

Deinking, modification of

Stability of dough and

Cheese flavoring Dairy Deinking, cleaning Textile

Conditioning of dough Baking Digestibility Animal feed Bleach boosting Paper and pulp

Scouring Textile

Softness of bread softness and

volume

fabrics

Glucose oxidase *Streptomyces coelicolor* Strengthening of dough Baking Keratinase *Nocradiopsis* sp. SD5 Feather degradation Animal feed Phytase *Streptomyces luteogriseus* R10 Phytate digestibility Animal feed

Deinking, drainage improvement

fructose syrups

Production of glucose and

Removal of starch from woven

Removal of stains Detergent

conditioning

Protease *Thermoactinomyces* sp.,

18 Actinobacteria - Basics and Biotechnological Applications

Cellulase *Streptomyces* sp.*, Thermobifida*

Xylanase *Actinomadura* sp., *Streptomyces* spp.

Amylase *Streptomyces* sp., *Streptomyces*

**Table 2.** Enzymes and their industrial applications

*erumpens,Nocardiopsis* sp. *,Thermobifida fusca, Nocardiopsis* sp.

*ruber*

*Nocardiopsis* sp., *Streptomyces pactum, Streptomyces*

*thermoviolaceus, Streptomyces* sp*.*

*halotolerans* , *Streptomyces* sp.*, Thermomonospora* sp.*, Streptomyces* Another interesting application of the Actinobacteria is the use of their secondary metabolites as herbicides against unwanted herbs and weeds. *Streptomyces saganonensis* produce herbici‐ dines and herbimycins that controls monocotyledonous and dicotyledonous weeds. Aniso‐ mycin, which is produced by *Streptomyces* sp., is a type of growth inhibitor for annual grassy weeds such as barnyardness and common crabgrass and broad-leaved weeds; anisomycin can destroy the ability of the plants to synthesize chlorophyll. Similarly, bialaphos, a metabolite of *Streptomyces viridochromogenes*, is widely used to control annual and perennial grassy weeds and broad-leaved weeds by inhibiting glutamine synthesis. Anisomycin can make small seedlings of barnyardness and common crabgrass die above 50 ppm and inhibit radicle growth below 12.5 ppm. Its synthetase may accumulate ammonia and control photosynthetic phos‐ phorylation, causing plant death [51]. *S. hygroscopicus* produce carbocyclic coformycin and hydantocidin, which can decrease synthetase of aclenylosuccinate by increasing the content of ATP and hold back the synthesis of protein [52]. In addition, phthoxazolin, hydantocidin, and homoalanosin from *Streptomyces* sp. can control several weeds [53]. Dhanasekaran et al [54] reported that *Streptomyces* sp. had the ability to inhibit the growth of *Echinochilora crusgalli*. Similarly, *Streptomyces* sp. KA1-3, KA1-4, KA1-7, and KA23A were found highly effective against *C. rotundus* [55]. Herbicidal activity of the bioactive compounds *N*-phenyl‐ propanamide and N (naphthalene-1-yl) propanamide from *Streptomyces* sp. KA1-3 [56, 57] was also evaluated.

#### **6.4. Probiotics**

Probiotics are the live microbial adjunct that has a beneficial effect on the host by various means, such as modifying the host associated or ambient microbial community, by ensuring the improved use of the feed or enhancing its nutritional value, by enhancing the host response towards disease, or by improving the quality of its ambient environment. Despite several other important applications, marine Actinobacteria have been given its attention for their use as probiotics. The potential of Actinobacteria against shrimp pathogenic *Vibrio* spp. made marine Actinobacteria as potential probiotic strains due to their ability to degrade macromolecules, such as starch and protein, in culture pond water; the production of antimicrobial agents; and the formation of heat- and desiccation-resistant spores [58]. Recently, a few studies were made on the possible use of marine Actinobacteria in disease prevention against aquatic pathogens. Das et al [59] in their preliminary study reported the use of *Streptomyces* sp. on the growth of black tiger shrimp. An antibiotic product extracted from marine Actinobacteria was incorpo‐ rated into feed to observe the *in vivo* effect on white spot syndrome virus in black tiger shrimp. Again, You et al [58] reported the activity of marine actinomycete as a potential organism against biofilms produced by *Vibrio* spp. and recommended the use of Actinobacteria to prevent the disease caused by *Vibrio* spp. In another study, Latha et al [36] screened 18 of the Actinobacteria isolated from chicken for probiotic properties, and the results revealed that *Streptomyces* sp. JD9 was the potent isolate with well-distinct probiotic properties.

#### *6.4.1. Aggregative peptide pheromones*

Aggregation is one of the most important criteria for the selection of a good probiotic candidate, which is the process of reversible accumulation of cells with one or more strains. For this aggregating process to take place, pheromone production is one of the main criteria that involves defense against predators, mate selection, and in overcoming host resistance by mass attack. In particular, sex pheromone peptides in culture supernatants have been shown to promote aggregation not only with the same species but also with related species [60–62]. Thus, the auto-aggregating ability of a probiotic is a prerequisite for colonization of the gastrointes‐ tinal tract, whereas coaggregation provides a close interaction with pathogenic bacteria. Though there are a number of studies in accordance with peptide pheromone–mediated signaling, it is lacking in the case of Actinobacteria, and thus a novel report on isolation and purification of diffusible aggregation promoting factor, that is, pheromones from potent Actinobacterial probiont *Streptomyces werraensis* LD22 isolated from the gut region of goat, were described by Muthu Selvam et al [63].The results clearly portray that Actinobacterial strain *S. werraensis* LD22 secretes a heat-stable, acidic pH–resistant, low molecular weight peptide pheromone that promotes the aggregation propensity and enhances the biofilm forming ability of other Actinobacterial isolates.

#### **6.5. Biosurfactants**

Biosurfactants are the microbially derived compounds that share hydrophilic and hydropho‐ bic moieties that are surface active. When compared with chemically derived surfactants, biosurfactants are independent of mineral oil as a feedstock; they are readily biodegradable and can be produced at low temperatures. Biosurfactants can be applied in various areas, such as the nutrient, cosmetic, textile, varnish, pharmaceutical, mining, and oil recovery industries [64–66]. The lipopeptide antibiotic daptomycin is an Actinobacterial biosurfactant that has already entered the market and is used in the treatment of diseases caused by Gram-positive pathogens and has been marketed as Cubicin by Cubist Pharmaceuticals. Diverse types of biosurfactants or bioemulsifiers have been described to be produced within the class *Actino‐ bacteria*. Among the best described biosurfactants are glucose-based glycolipids, most of which have a hydrophilic backbone consisting of glycosidic-linked glucose units forming a trehalose moiety.

#### **6.6. Vitamins**

Vitamin B12 as it exists in nature may be produced by bacteria or Actinobacteria [67]. Isolation of vitamin B12 from Actinobacteria fermentations [68, 69] stirred up considerable interest in possible production of vitamin by microbial fermentations. Addition of cobalt salts to the media apparently acts as a precursor for all Actinobacteria to produce vitamin. As cobalt is a rather effective bactericidal agent, this precursor must be added carefully. The fermentations producing the antibiotics streptomycin, aureomycin, grisein, and neomycin will produce some vitamin B12 as well if the medium is supplemented with cobalt without apparently affecting the yields of antibiotic substances. Several other studies suggested that some Actinobacteria that are non–antibiotic-producing cultures produce more of this vitamin than those producing antibiotics. Actinobacteria have been shown to produce other water soluble vitamins, with special studies on production of thiamine and the pteroylglutamic acid derivative that is active in promoting the growth of certain strains of *Leuconostoc citrovorum* and coenzyme A.

### **6.7. Pigments**

*6.4.1. Aggregative peptide pheromones*

20 Actinobacteria - Basics and Biotechnological Applications

forming ability of other Actinobacterial isolates.

**6.5. Biosurfactants**

moiety.

**6.6. Vitamins**

Aggregation is one of the most important criteria for the selection of a good probiotic candidate, which is the process of reversible accumulation of cells with one or more strains. For this aggregating process to take place, pheromone production is one of the main criteria that involves defense against predators, mate selection, and in overcoming host resistance by mass attack. In particular, sex pheromone peptides in culture supernatants have been shown to promote aggregation not only with the same species but also with related species [60–62]. Thus, the auto-aggregating ability of a probiotic is a prerequisite for colonization of the gastrointes‐ tinal tract, whereas coaggregation provides a close interaction with pathogenic bacteria. Though there are a number of studies in accordance with peptide pheromone–mediated signaling, it is lacking in the case of Actinobacteria, and thus a novel report on isolation and purification of diffusible aggregation promoting factor, that is, pheromones from potent Actinobacterial probiont *Streptomyces werraensis* LD22 isolated from the gut region of goat, were described by Muthu Selvam et al [63].The results clearly portray that Actinobacterial strain *S. werraensis* LD22 secretes a heat-stable, acidic pH–resistant, low molecular weight peptide pheromone that promotes the aggregation propensity and enhances the biofilm

Biosurfactants are the microbially derived compounds that share hydrophilic and hydropho‐ bic moieties that are surface active. When compared with chemically derived surfactants, biosurfactants are independent of mineral oil as a feedstock; they are readily biodegradable and can be produced at low temperatures. Biosurfactants can be applied in various areas, such as the nutrient, cosmetic, textile, varnish, pharmaceutical, mining, and oil recovery industries [64–66]. The lipopeptide antibiotic daptomycin is an Actinobacterial biosurfactant that has already entered the market and is used in the treatment of diseases caused by Gram-positive pathogens and has been marketed as Cubicin by Cubist Pharmaceuticals. Diverse types of biosurfactants or bioemulsifiers have been described to be produced within the class *Actino‐ bacteria*. Among the best described biosurfactants are glucose-based glycolipids, most of which have a hydrophilic backbone consisting of glycosidic-linked glucose units forming a trehalose

Vitamin B12 as it exists in nature may be produced by bacteria or Actinobacteria [67]. Isolation of vitamin B12 from Actinobacteria fermentations [68, 69] stirred up considerable interest in possible production of vitamin by microbial fermentations. Addition of cobalt salts to the media apparently acts as a precursor for all Actinobacteria to produce vitamin. As cobalt is a rather effective bactericidal agent, this precursor must be added carefully. The fermentations producing the antibiotics streptomycin, aureomycin, grisein, and neomycin will produce some vitamin B12 as well if the medium is supplemented with cobalt without apparently affecting the yields of antibiotic substances. Several other studies suggested that some Actinobacteria

As synthetic dyes have some limitations such as usage of hazardous chemicals for their production, creating worker safety concerns and generation of hazardous wastes, microbeoriented pigments are of great concern. Specially, Actinobacteria are characterized by the production of various pigments on natural or synthetic media (Figure 8) and are considered as an important cultural characteristic in describing the organisms. Any phenotypic changes induced by environmental influences will help Actinobacteria as they boast distinctive colony morphologies and produce variety of pigments and aerial branching filaments called hyphae, which give them a characteristic fuzzy appearance. These pigments usually comes in various shades of blue, violet, red, rose, yellow, green, brown, and black, which may be dissolved into the medium or it may be retained in the mycelium. The pigments produced by *Streptomyces* may be either endopigments (bound to certain cell structures) or exopigments (excreated into the surrounding medium). Sometimes different antibiotics produced by the Actinobacteria are considered as pigments. Since the formation of pigment is influenced by the pH of the medium, aeration, temperature of the growth, and carbon and nitrogen sources, only a little is known about the exact chemical nature of pigments. Its formation is also linked to respiratory mechanisms, defense mechanisms, and ultraviolet protection. These microbes also have the ability to synthesize and excrete dark pigments, melanin or melanoid, which are considered to be a useful criterion for taxonomical studies. The textile industry produces and uses approximately 1.3 million tonnes of dyes, pigments, and dye precursors, valued at around \$23 billion, almost all of which are manufactured synthetically. Table 3 provides a list of pigments from different Actinobacteria.

**Figure 8.** Diffusible pigment produced by various Actinobacteria in starch casein agar medium.


**Table 3.** Pigments from Actinobacteria

### **6.8. Nanoparticle synthesis**

Nanoparticles are of great scientific interest as they bridge the gap between bulk materials and atomic or molecular structures. Generally, the chemical methods are low cost for high volume; however, their drawbacks include contamination from precursor chemicals, use of toxic solvents, and generation of hazardous byproducts. Hence, there is an increasing need to develop high-yield, low-cost, nontoxic, and environmentally benign procedures for synthesis of metallic nanoparticles. Therefore, the biological approach for synthesis of nanoparticles becomes important. In fact, Actinobacteria are efficient producers of nanoparticles, which show a range of biological properties, namely antibacterial, antifungal, anticancer, antibio‐ fouling, antimalarial, antiparasitic, and antioxidant. *Streptomyces* and *Arthrobacter* genera have been studied as possible "nanofactories" for the development of clean and nontoxic methods of the synthesis of silver and gold nanoparticles. A recent example of silver nanoparticle synthesis from an Actinobacteria *Streptomyces* sp. GRD was performed by Gopinath et al [70]. Ranjani et al [71] observed the diversity of silver nanoparticle synthesizing Actinobacteria from marine environment and showed that 25 isolates of 49 synthesized silver nanoparticles, the genus of which includes *Streptomyces* sp., *Nocardiopsis* sp., *Kitasatosporia* sp., *Actinopolyspora* sp., *Thermoactinomyces* sp., *Actinomadura* sp., *Kibdelosporangium* sp., *Saccharopolyspora* sp., and *Thermomonospora* sp. Table 4 shows the synthesis of nanoparticles using various genera.


**Table 4.** List of nanoparticles synthesized using Actinobacteria

#### **6.9. Bioremediation**

Actinobacteria possess many properties that make them good candidates for application in bioremediation of soils contaminated with organic pollutants. In some contaminated sites, Actinobacteria represent the dominant group among the degraders [72]. They play an important role in the recycling of organic carbon and are able to degrade complex poly‐ mers. Sanscartier et al [73] reported that the greater use of petroleum hydrocarbons that are widely used in our daily life as chemical compounds and fuel has become one of the most common contaminants of large soil surfaces and eventually is considered as a major environmental problem. Some reports suggests that *Streptomyces* flora could play a very important role in degradation of hydrocarbons [74, 75]. Many Actinobacterial strains have the ability to solubilize lignin and degrade lignin-related compounds by producing cellu‐ lose- and hemicellulose-degrading enzymes and extracellular peroxidase [76]. Actinobacte‐ ria species have the ability to live in an oily environment and thus they can be used in bioremediation to reduce oil pollutants. *Nocradiopsis* sp. SD5 degraded feather waste by producing keratinase enzyme [50].

#### **6.10. Control of plant diseases**

**Pigment Class Actinobacteria**

Actinomycin Phenoxazinone *Streptomyces* sp*.*

III Undecylprodigiosin IV Metacycloprodigiosin

**Table 3.** Pigments from Actinobacteria

22 Actinobacteria - Basics and Biotechnological Applications

**6.8. Nanoparticle synthesis**

Rhodomycin Anthracycline glycoside *Synodontis violaceus* DSM 40704

Granaticin Naphthoquinone *Streptomyces litmocidin* DSM 40164

**Actinobacteria Nanoparticles**

*Streptomyces* sp. Zinc, copper, manganese

Actinobacteria possess many properties that make them good candidates for application in bioremediation of soils contaminated with organic pollutants. In some contaminated sites, Actinobacteria represent the dominant group among the degraders [72]. They play an

*Streptomyces* sp., *Thermoactinomyces* sp., *Nocardiopsis* sp., *Rhodococcus* sp., *Streptomyces*

*Streptomyces aureofaciens, Actinobacteria,Streptomyces glaucus, Streptomyces viridogens, Thermoactinomycete* spp., *Thermomonospora* spp., *Nocardia farcinica, Streptomyces*

*albidoflavus, Streptomyces hygroscopicus, Streptomyces rochei*

**Table 4.** List of nanoparticles synthesized using Actinobacteria

*hygroscopicus*

**6.9. Bioremediation**

Nanoparticles are of great scientific interest as they bridge the gap between bulk materials and atomic or molecular structures. Generally, the chemical methods are low cost for high volume; however, their drawbacks include contamination from precursor chemicals, use of toxic solvents, and generation of hazardous byproducts. Hence, there is an increasing need to develop high-yield, low-cost, nontoxic, and environmentally benign procedures for synthesis of metallic nanoparticles. Therefore, the biological approach for synthesis of nanoparticles becomes important. In fact, Actinobacteria are efficient producers of nanoparticles, which show a range of biological properties, namely antibacterial, antifungal, anticancer, antibio‐ fouling, antimalarial, antiparasitic, and antioxidant. *Streptomyces* and *Arthrobacter* genera have been studied as possible "nanofactories" for the development of clean and nontoxic methods of the synthesis of silver and gold nanoparticles. A recent example of silver nanoparticle synthesis from an Actinobacteria *Streptomyces* sp. GRD was performed by Gopinath et al [70]. Ranjani et al [71] observed the diversity of silver nanoparticle synthesizing Actinobacteria from marine environment and showed that 25 isolates of 49 synthesized silver nanoparticles, the genus of which includes *Streptomyces* sp., *Nocardiopsis* sp., *Kitasatosporia* sp., *Actinopolyspora* sp., *Thermoactinomyces* sp., *Actinomadura* sp., *Kibdelosporangium* sp., *Saccharopolyspora* sp., and *Thermomonospora* sp. Table 4 shows the synthesis of nanoparticles using various genera.

Prodigiosin *Streptomyces longispororuber* DSM 40599

Silver

Gold

The worldwide efforts in the search of natural products for the crop protection market have progressed significantly, and Actinobacteria, especially genus *Streptomyces*, appear to be good candidates in finding new approaches to control plant diseases. The agroindustry shows a marked interest for Actinobacteria as a source of agroactive compounds of plant growth– promoting rhizobacteria (PGPR) and of biocontrol tools [77, 78]. About 60% of the new insecticides and herbicides reported in the past 5 years originate from *Streptomyces* [78]. Kasugamycin is a bactericidal and fungicidal metabolite discovered in *Streptomyces kasugaen‐ sis* [79], which acts as an inhibitor of protein biosynthesis in microorganisms but not in mammals, and its toxicological properties are excellent. To market the systemically active kasugamycin for control of rice blast *Pyricularia oryzae* and bacterial *Pseudomonas* diseases in several crops, Hokko Chemical Industries developed a production process. Polyoxin B and D were isolated as metabolites of *Streptomyces cacaoi* var. *asoensis* in 1965 by Isono et al [80] as a new class of natural fungicides. The ability of the polyoxins to interfere with the fungal cell wall synthesis by specifically inhibiting chitin synthase [81] makes them acceptable with regard to environmental considerations. Polyoxin B found application against a number of fungal pathogens in fruits, vegetables, and ornamentals. Polyoxin D is marketed by several companies to control rice sheath blight caused by *Rhizoctonia solani*. The validamycin family was detected by Takeda researchers in 1968 in a greenhouse assay when screening streptomy‐ cete extracts for activity against rice sheath blight. Validamycin A was found to be a prodrug, which is converted within the fungal cell to validoxylamine A, an extremely strong inhibi‐ tor of trehalase [82]. This mode of action gives validamycin A a favorable biological selectivity because vertebrates do not depend on the hydrolysis of the disaccharide trehalose for their metabolism. Inhibition of plant pathogenic *Rhizoctonia solani* under *in vitro* condition was assessed with the culture supernatant of *Streptomyces* sp., which showed that the tested Actinobacteria had the ability to reduce damping off severity in tomato plants. Table 5 represents some of the antibiotics produced by the Actinobacteria that suppresses various plant diseases.


**Table 5.** Plant disease suppression by antibiotics produced by Actinobacteria

#### **6.11. Nematode control**

It has been known for decades that effective control of plant-parasitic nematodes is dependent on chemical nematicides. Due to its ill effects with respect to the environmental hazards, hazardous nematicides have emphasized the need for new methods to control nematodes. Today, numerous microorganisms are recognized as antagonists of plant-parasitic nematodes. Especially, Actinobacteria have potential for use in biological control as they are known to produce antibiotics. The production of avermectins by a species of *Streptomyces* shows that soil-borne organisms can produce highly nematicidal compounds. *S. avermitilis* produces ivermectin, which has an excellent activity against *Wucheria bancroftii* [83]. Similarly various other antiparasitic compounds are produced from different *Streptomyces* sp*., Salinispora* sp*.,* and *Marinactinospora* sp*.*, which includes *Milbemycin, Antimycin* A9*, Fervenulin, bafilolides, Valinomycin, Salinosporamide* A*, Kalafungin, Thiamycins*, *and Axenomycins.*

### **6.12. Enhancement of plant growth**

**Disease Actinobacteria Antibiotic produced**

Grass seedling disease *Streptomyces violaceusniger* YCED9 Nigericin and guanidylfungin A

Geldanamycin

Validamycin

phenylacetic acid, cervinomycin

A1 and A2

EF-76 and FP-54

Root rot of Pea *Streptomyces hygroscopicus* var*. geldanus* Geldanamycin Asparagus root diseases *Streptomyces griseus* Faeriefungin Rice blast disease *Streptomyces kasugaensis* Kasugamycin Broad range of plant diseases *Streptomyces griseochromogenes* Blasticidin S

Potato scab *Streptomyces melanosporofaciens*

24 Actinobacteria - Basics and Biotechnological Applications

Sheath blight of rice *Streptomyces hygroscopicus* var. *limoneus*

No. T-7545

Brown rust of wheat *Streptomyces hygroscopicus* Gopalamycin Phytophthora blight of pepper *Streptomyces violaceusniger* Tubercidin

Phytophthora blight of pepper *Streptomyces humidus* Phenylacetic Acid Damping-off of cabbage *Streptomyces padanus* Fungichromin Rice sheath blight *Streptomyces cacaoi* var. *asoensis* Polyoxin B and D Powdery mildew *Streptoverticillium rimofaciens* Mildiomycin Rice root disease *Micromonospora* sp*.* SF-1917 Dapiramicin

Rice blast *Micromonospora* sp. M39 2,3-dihydroxybenzoic acid,

It has been known for decades that effective control of plant-parasitic nematodes is dependent on chemical nematicides. Due to its ill effects with respect to the environmental hazards, hazardous nematicides have emphasized the need for new methods to control nematodes. Today, numerous microorganisms are recognized as antagonists of plant-parasitic nematodes. Especially, Actinobacteria have potential for use in biological control as they are known to produce antibiotics. The production of avermectins by a species of *Streptomyces* shows that soil-borne organisms can produce highly nematicidal compounds. *S. avermitilis* produces ivermectin, which has an excellent activity against *Wucheria bancroftii* [83]. Similarly various other antiparasitic compounds are produced from different *Streptomyces* sp*., Salinispora* sp*.,* and *Marinactinospora* sp*.*, which includes *Milbemycin, Antimycin* A9*, Fervenulin, bafilolides,*

Blotch of wheat *Streptomyces malaysiensis* Malayamycin Powdery mildew of cucumber *Streptomyces* sp. KNF2047 Neopeptin A and B

*Valinomycin, Salinosporamide* A*, Kalafungin, Thiamycins*, *and Axenomycins.*

**Table 5.** Plant disease suppression by antibiotics produced by Actinobacteria

**6.11. Nematode control**

Despite the well-documented history of *Streptomyces* in biocontrol and preliminary evidence of their capacity to enhance plant growth [84], *Streptomyces* species have been poorly investi‐ gated specifically for their potential as PGPR. While the beneficial effect of some strains of PGPR on particular crops is certain, the mechanisms employed by PGPR are unclear [85]. PGPR can affect plant growth in two general ways, either directly or indirectly. Indirect promotion occurs when PGPR lessen or prevent the harmful effects of one or more deleterious microor‐ ganisms. This is chiefly attained through biocontrol or the antagonism of soil plant pathogens. Specifically, colonization or the biosynthesis of antibiotics and other secondary metabolites can prevent pathogen invasion and establishment. Direct promotion of plant growth by PGPR occurs when the plant is supplied with a compound that is synthesized by the bacteria, or when PGPR otherwise facilitates plant uptake of soil nutrients. Merriman et al [86] reported the use of *S. griseus* for seed treatment of barley, oat, wheat, and carrot to increase their growth. The isolate was originally selected for the biological control of *Rhizoctonia solani*. Though the *S. griseus* isolate did increase the average grain yield, dry foliage weight, tiller number, and advanced head emergence for both wheat and oat over controls, the differences were not statistically significant. As a seed treatment for carrot, the isolate was more successful. Marketable yields were increased over controls by 17% and 15% in two separate field trials. Specifically, both trials also indicated an increased yield of large and very large grade carrots over controls [86]. El-Abyad et al [87] described the use of three *Streptomyces* spp. in the control of bacterial, *Fusarium* and *Verticillium* wilts, early blight, and bacterial canker of tomato. The isolates used were *Streptomyces pulcher*, *Streptomyces canescens*, and *Streptomyces citreofluores‐ cens*. In addition, tomato growth was observed to be significantly improved with the antago‐ nistic *Streptomyces* spp. as a seed coating. An increased availability of growth regulators produced by the inoculum was the reason proposed for the improvement in tomato growth, although this was not formally tested. The information available on streptomycetes as plant growth promoters is limited, so is the information describing the possibility of their direct growth promotion mechanisms. Like most rhizobacteria, it seems highly probable that streptomycetes are capable of directly enhancing plant growth.

#### **6.13. Phytohormone production**

The production of the plant hormone indole-3-acetic acid (IAA) and the pathways of its synthesis by various *Streptomyces* sp., including *S. violaceus*, *Streptomyces scabies*, *S. griseus*, *Streptomyces exfoliatus*, *S. coelicolor*, and *S. lividans,* were described by Manulis et al [88]. While prior works had reported IAA synthesis in *Streptomyces* spp., this was the first confirmation of its production using modem analytical methods, such as high-performance liquid chroma‐ tography (HPLC) and gas chromatography (GC)–mass spectrometry (MS), and Manulis et al [88] provided a detailed description of the IAA biosynthetic pathways in *Streptomyces.* Aldesuquy et al [84] studied the effect of streptomycete culture filtrates on the growth of wheat plants that showed significant increase in shoot fresh mass, dry mass, length, and diameter, displayed statistically with certain strains at varying sample times. *Streptomyces olivaceoviri‐ dis* had a pronounced effect on yield components (spikelet number, spike length, and fresh and dry mass of the developing grain) of wheat plants. This activity may be due to, at least in part, an increase in bioavailable phytohormones that are PGPR produced since ail PGPR strains (*Streptomyces rimosus*, *Streptomyces rochei* and *S. olivaceoviridis*) produced substantial amounts of exogenous auxins (IAA), as well as gibberellins and cytokinins.

### **6.14. Biolarvicides**

Extensive use of chemical insecticides for controlling malaria, filaria, dengue, chickungunya, Japanese encephalitis, and other mosquitoes have resulted in hazards to the environment and caused development of resistance in vector mosquitoes. Accordingly, various biological control agents have gained importance with innumerable advantages over the chemical insecticides. At very low doses, these biolarvicides are highly effective against mosquito larvae and are completely safe to other nontarget organisms, environment, man, and wild life. Several varieties of microorganisms, including fungi, bacteria, and nematodes have been reported as strategies to biologically control the vectors. Specifically, Actinobacteria produce many important bioactive compounds of high commercial value and continue to be routinely screened for new bioactive substances. In a study made by Vijayan and Balaraman [89], extracellular secondary metabolites were produced from 35 different Actinobacterial isolates that showed high larvicidal activity against *Culex* and *Anopheles* mosquitoes. Dhanasekaran et al [20] reported that the isolates *Streptomyces* sp., *Streptosporangium* sp., and *Micropolyspora* sp. had high larvicidal activity against *Anopheles* mosquito larvae. Rajesh et al [90] synthesized silver nanoparticles from *Streptomyces* sp. GRD cell filtrate and observed larvicidal activity against *Aedes* and *Culex* vectors, which are responsible for the transmission of dengue and filariasis. Yet again, Rajesh et al [91] studied the larvicidal effect of Actinobacterial extracts against *Culex* larvae and found that 1000 ppm concentration of the isolate *Streptomyces* sp. KA13-3 showed 100% mortality and *Streptomyces* sp. KA25-A showed 90% mortality. Variety of other secondary metabolites from Actinobacteria, namely tetranectin [92], avermectins [93], macrotetrolides [94], and flavonoids [95] were found to be toxic to mosquitoes.

#### **6.15. Odor and flavor compounds production**

Actinomycetes have long been associated with musty odors in water but their actual contri‐ bution to odor in freshwater was unknown. But in late 1960s, secondary metabolites, geosmin and 2-methylisoborneol (MIB), were identified from actinomycete cultures [96] after which actinomycetes have gained considerable importance throughout the water industry as major sources of drinking water taste and odor. Gaines and Collins [97] studied the metabolites of *Streptomyces odorifer* and concluded that the earthy odor might be due to the production of a combination of trivial compounds, such as acetic acid, acetaldehyde, ethyl alcohol, isobutyl alcohol, isobutyl acetate, and ammonia. They emphasized that the other constituents contri‐ buting to the odor might also be produced. A number of odor-producing compounds have been identified from Actinobacteria (Table 6). Earthy odors in adequately treated water supplies cause concern among consumers, who may think water with these odors is unsafe to drink. These odors are the second most common cause of odor problems recorded by water utilities, behind chlorine.


**Table 6.** Odor-producing compounds from Actinobacteria

### **7. Harmful effects**

and dry mass of the developing grain) of wheat plants. This activity may be due to, at least in part, an increase in bioavailable phytohormones that are PGPR produced since ail PGPR strains (*Streptomyces rimosus*, *Streptomyces rochei* and *S. olivaceoviridis*) produced substantial amounts

Extensive use of chemical insecticides for controlling malaria, filaria, dengue, chickungunya, Japanese encephalitis, and other mosquitoes have resulted in hazards to the environment and caused development of resistance in vector mosquitoes. Accordingly, various biological control agents have gained importance with innumerable advantages over the chemical insecticides. At very low doses, these biolarvicides are highly effective against mosquito larvae and are completely safe to other nontarget organisms, environment, man, and wild life. Several varieties of microorganisms, including fungi, bacteria, and nematodes have been reported as strategies to biologically control the vectors. Specifically, Actinobacteria produce many important bioactive compounds of high commercial value and continue to be routinely screened for new bioactive substances. In a study made by Vijayan and Balaraman [89], extracellular secondary metabolites were produced from 35 different Actinobacterial isolates that showed high larvicidal activity against *Culex* and *Anopheles* mosquitoes. Dhanasekaran et al [20] reported that the isolates *Streptomyces* sp., *Streptosporangium* sp., and *Micropolyspora* sp. had high larvicidal activity against *Anopheles* mosquito larvae. Rajesh et al [90] synthesized silver nanoparticles from *Streptomyces* sp. GRD cell filtrate and observed larvicidal activity against *Aedes* and *Culex* vectors, which are responsible for the transmission of dengue and filariasis. Yet again, Rajesh et al [91] studied the larvicidal effect of Actinobacterial extracts against *Culex* larvae and found that 1000 ppm concentration of the isolate *Streptomyces* sp. KA13-3 showed 100% mortality and *Streptomyces* sp. KA25-A showed 90% mortality. Variety of other secondary metabolites from Actinobacteria, namely tetranectin [92], avermectins [93],

macrotetrolides [94], and flavonoids [95] were found to be toxic to mosquitoes.

Actinomycetes have long been associated with musty odors in water but their actual contri‐ bution to odor in freshwater was unknown. But in late 1960s, secondary metabolites, geosmin and 2-methylisoborneol (MIB), were identified from actinomycete cultures [96] after which actinomycetes have gained considerable importance throughout the water industry as major sources of drinking water taste and odor. Gaines and Collins [97] studied the metabolites of *Streptomyces odorifer* and concluded that the earthy odor might be due to the production of a combination of trivial compounds, such as acetic acid, acetaldehyde, ethyl alcohol, isobutyl alcohol, isobutyl acetate, and ammonia. They emphasized that the other constituents contri‐ buting to the odor might also be produced. A number of odor-producing compounds have been identified from Actinobacteria (Table 6). Earthy odors in adequately treated water supplies cause concern among consumers, who may think water with these odors is unsafe to drink. These odors are the second most common cause of odor problems recorded by water

**6.15. Odor and flavor compounds production**

utilities, behind chlorine.

of exogenous auxins (IAA), as well as gibberellins and cytokinins.

**6.14. Biolarvicides**

26 Actinobacteria - Basics and Biotechnological Applications

### **7.1. Actinobacterial plant diseases**

A number of significant plant diseases are caused by Actinobacteria. Actinobacteria currently assigned to the genus *Corynebacterium* [98] cause a variety of diseases (Table 7). Serodiagnosis has been used to detect and identify *Corynebacterium insidiosum* and *Corynebacterium sependo‐ nicum*, but most of these Corynebacteria are still identified by inoculation tests in host plants. Most phytopathogenic Corynebacteria are considered to produce their antagonistic effects by the production of hormones, polysaccharides, and toxins, whereas the potential of some strains to form biosurfactants may help in attachment to the host. The taxonomy of the phytopatho‐ genic Corynebacteria is unsettled, though some prefer to retain these organisms in the genus *Corynebacterium* [99]. Others accept that there is an overwhelming case for restricting *Coryne‐ bacterium* for animal pathogenic Corynebacteria and related strains and a consequent need to reclassify the plant pathogens. *Corynebacterium betae, Corynebacterium oortii,* and *Corynebacte‐ rium poinsettiae* are genetically identified with *Corynebacterium flaccumfaciens* [100] and have many properties in common with the genus *Curtobacterium* [100], which would serve as a suitable niche for them. *C. insidiosum* and *Corynebacterium michiganense*, also fell into a single genospecies, were recovered in a loose DNA homology group with *Corynebacterium nebras‐ kense* and *Corynebacterium sepedonicum* and together with the latter probably from the nucleus of a new genus.

#### **7.2. Actinobacterial human and animal diseases**

Actinobacteria have proved to be the causal agents of many human and animal infections, which include a number of common and intensively studied diseases, such as diphtheria, tuberculosis, and leprosy. There is also a wide range of infections that are less well known;


#### **Table 7.** Plant diseases caused by Actinobacteria

some, like actinomycosis and nocardiosis, are proving to be more clinically significant than previously thought. In addition, it is becoming increasingly evident that *Actinomyces* play a role in the etiology of caries and periodontal disease. Following are the human and animal diseases caused by Actinobacteria (Table 8).



**Table 8.** Human and animal diseases caused by Actinobacteria

### **8. Conclusion**

some, like actinomycosis and nocardiosis, are proving to be more clinically significant than previously thought. In addition, it is becoming increasingly evident that *Actinomyces* play a role in the etiology of caries and periodontal disease. Following are the human and animal

*C. michiganense* Canker of tomato (*Lycopersicon esculentum*) and some other solanaceous plants

*Corynebacterium Poinselliae* Stem canker and leaf spot of poinsettia (*Euphorbia pulcherrima*)

Feet, legs, upper extremities, and other

Cervicofacial, thoracic, abdominal, and

sites

uterine regions

internal organs

Lymphatic system

**Disease Actinobacteria Site of infection**

*Nippostrongylus brasiliensis, N.*

*Arachnia propionica*

*otitidiscaviarum, Streptomyces somaliensis*

Bacterial kidney disease *Renibacterium salmoninarium* Kidney, liver, spleen, and other

Diphtheria *Corynebacterium diphtheriae* Throat, occasionally wounds

Endocarditis *Oerskovia turbata, Rothia dentocariosa* Endocardium

*Dermatophilis congolensis* Skin

diseases caused by Actinobacteria (Table 8).

**Table 7.** Plant diseases caused by Actinobacteria

*Arthrobacter ilicis* B light of holly (flex opaca)

28 Actinobacteria - Basics and Biotechnological Applications

*C. nebraskense* Wilt and blight of corn

*Corynebacterium rathayi* Gumming of cereals

*S. aureofaciens S. flaveolus. S. griseus* Common scab of potato *S. ipomoeae* Sweet potato scab

*C. oortii* Spot of tulip leaves and bulbs

*C. flaccumfaciens* Wilt of bean (*Phaseolus vulgaris*)

*C. betae* Wilt and leaf spot of red beet (*Beta vulgaris*)

*C. insidiosum* Wilt and stunting of alfalfa (*Medicago sativum*)

*C. sepedonicum* Wilt and tuber rot of potato (*Solanum tuberosum)*

*Rhodococcus fascians* Leaf gall in many plants, fasciation of sweet pea

*S. scabies, Streptomyces sp.* Common and russet scab of potatoes, sugar beet, etc

plants (*Vaccinium*)

*Nocardia vaccinii* Galls and bud proliferation in blueberry

Dermatophilosis & streptothricosis

Actinomycetoma *A. madurae, pellerieri, Nocardia asteroides,*

Actinomycosis *Actinomyces bovis, Actinomyces israelii,*

Bovine farcy *Mycobacterium farcinogenes, Mycobacterium senegalense*

Actinobacteria is one of the dominant groups of microorganisms that produce industrially important secondary metabolites. A wide range of antibiotics in the market is obtained from Actinobacteria. Products such as enzymes, herbicides, vitamins, pigments, larvicides, phyto‐ hormones, and surfactants are produced by these several genera of Actinobacteria, which are of great commercial value. They are capable of degrading a wide range of hydrocarbons, pesticides, and feather waste, and their metabolic potential offers a strong area for research. However, many of the rare genera of Actinobacteria have been neither discovered from unexplored locations nor employed for their biotechnological and industrial potential. Thus, studies on unique ecological environments could yield molecules that could become future harbingers of green technology.

### **Author details**

Ranjani Anandan, Dhanasekaran Dharumadurai\* and Gopinath Ponnusamy Manogaran

\*Address all correspondence to: dhansdd@gmail.com

Department of Microbiology, School of Life Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India

### **References**


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