**2.3.2 Reverse transcription**

Total RNA (1 µg) was denatured for 5 min at 65°C and mRNAs were reverse-transcripted (60 min at 37°C) in 25 µL final volume containing 5 µL of M-MLV RT 5X buffer, 100 U moloney murine leukemia virus reverse transcriptase (Invitrogen, France), 15 nmoles of deoxynucleotide triphosphate and 1 µg oligo dT (PCR) or 100 ng random primer (qPCR, Invitrogen). After RT, inactivation step was 15 min at 70°C. RT products were frozen (-30°C).
