**Meet the editor**

Dr. Xu-bo Yuan, Ph.D., is a nanoparticles (drug and gene delivery) researcher in Tianjin University, China. Dr. Yuan graduated from the Beijing Institute of Science & Technology in 1989 with a degree in Chemical Engineering. He received his M.S. and Ph.D. from Tianjin University in 1995 and 2001, respectively. After doing his poster doctoral work at Institute of Polymer Chemistry,

Nankai University, he was an associate professor at Tianjin University, and a visiting scientist in the Department of Chemistry and Biomolecular Engineering at the University of California, Los Angeles. He is currently the professor working on the co-delivery of chemotherapeutant and miRNA in Tianjin University.

Contents

**Preface IX** 

Chapter 3 **Gene Delivery Systems:** 

Chapter 1 **Non-Viral Gene Therapy Vectors** 

Chapter 2 **Non-Viral Delivery Systems in Gene** 

Azam Bolhassani and Sima Rafati

Stefanie Häfele and Regine Süss

Chapter 5 **The Mechanical Agitation Method of Gene** 

**and Stem Cells from Patients 105** 

Chapter 8 **DNA Vaccination by Electrogene Transfer 169** 

Pieranna Chiarella, Vito Michele Fazio and Emanuela Signori

Chapter 7 **Non Viral Gene Transfer Approaches for Lysosomal Storage Disorders 147**  Ursula Matte, Guilherme Baldo

and Roberto Giugliani

and Seong-Tshool Hong

Liting Song

**Transfer for** *Ex-Vivo* **Gene Therapy 91**  Hea-Jong Chung, Hyun-Seo Lee, Hyeon-Jin Kim

Chapter 6 **Gene Therapy of Some Genetic Diseases by Transferring Normal Human Genomic DNA into Somatic Cells** 

**Carrying Genomic Constructs 1**  George Kotzamanis, Hassan Abdulrazzak, Athanassios Kotsinas and Vassilis G. Gorgoulis

**Therapy and Vaccine Development 27** 

**Tailoring Vectors to Reach Specific Tissues 51**  Carlos J. Alméciga-Díaz, Rocio Cuaspa and Luis A. Barrera

Chapter 4 **Investigation of Transfection Barriers Involved in Non-Viral** 

**Nanoparticulate Gene Delivery in Different Cell Lines 77** 
