**6.1.4 Scalable top-down process for liposome production**

The scalable process described in Figure 11 was established based on these results and validated for the production of EPC/DOPE/DOTAP liposomes. Previous assays (data not shown) validated the changes in the purity of EPC from 99-100% (analytical grade) to 96- 98% (commercial grade) and the lipid concentration from 16 mM (used in the Bangham's method) to 64 mM.

The injection of ethanol was used modified for the higher lipid concentration. Lipid concentration in the ethanol phase was previously optimized with an aim to minimize the concentration of ethanol in the liposome formulation, as well as prevent obstructions of the microchannels in the microfluidizer.

Fig. 11. Schematic diagram of the scalable process for cationic liposome production.
