**5. Conclusion**

PI polyamides show a remarkable potential for use in non viral gene therapy as many attractive results were obtained. The novel compounds could provide a promising impact on gene therapy for diseases not treatable by current remedies. To obtain the maximum therapeutic effect of the PI polyamide, it is crucial to evaluate the pharmacokinetics of the compounds for designing appropriate dosage regimens. Bioanalytical procedures for PI polyamides A and B were successfully developed and validated by HPLC or LC-MS/MS, and applied to sample assay. The pharmacokinetic profiles of PI polyamides show interesting results, which are thought to be related to their molecular weights (Brown et al., 1981). It was suggested that the features of various compositions of Py and Im were related to their unique pharmacokinetic profiles. Further examination will be conducted using other PI polyamides that have unique Py and Im combinations for gene therapy.
