**Preface XIII**


X Contents


Contents VII

Chapter 21 **PAMAM Dendrimer as Potential Delivery System for Combined Chemotherapeutic and MicroRNA-21 Gene Therapy 499** Xuan Zhou, Yu Ren, Xubo Yuan, Peiyu Pu and Chunsheng Kang

**Cancer Diagonsis and Therapy 515**

**Gene Delivery Nanosystems 547** Yadollah Omidi, Vala Kafil and Jaleh Barar

**as a Gene Delivery System 577** Babak Mostaghaci, Arash Hanifi, Brigitta Loretz and Claus-Michael Lehr

**Vectors for Gene Therapy 597**

**Vectors for Gene Therapy 615** Wei Yang Seow and Andrew JT George

**Entities onto Synthetic Vesicles 645**  Federica De Persiis, Ramon Pons, Carlotta Pucci,

Cristiana Lavazza and Alessandro M. Gianni

Chapter 29 **Pyrrole-Imidazole Polyamides for Gene Therapy:** 

**Engineered CD34+ Hematopoietic Stem Cells 661**  Carmelo Carlo-Stella, Arianna Giacomini, Silvia L. Locatelli,

**Bioanalytical Methods and Pharmacokinetics 679**  Tomonori Kamei, Takahiko Aoyama, Takahiro Ueno, Noboru Fukuda, Hiroki Nagase and Yoshiaki Matsumoto

Franco Tardani and Camillo La Mesa

Chapter 28 **Targeting TRAIL Receptors with Genetically-**

Roderick A. Slavcev, Shawn Wettig and Tranum Kaur

Chapter 22 **Nanomedicine Based Approaches to** 

Chapter 23 **Toxicogenomics of Nonviral Cationic**

Chapter 24 **Nano-Particulate Calcium Phosphate** 

Chapter 25 **Modular Multifunctional Protein**

Chapter 26 **Peptides as Promising Non-Viral** 

Chapter 27 **Binding of Protein-Functionalized** 

Hugo Peluffo



VI Contents

Chapter 9 **Gene Delivery with Ultrasound and Microbubbles 199** 

Evan Unger and Terry Matsunaga

Chapter 10 **Ultrasound-Mediated Gene Delivery 213**  Carol H. Miao and Andrew A. Brayman

Chapter 11 **Polyamine – Lipid Conjugates as Effective Gene Carriers: Chemical Structure, Morphology,**

Chapter 12 **Technological Aspects of Scalable Processes for the** 

**Structural Levels for Gene Delivery 293** Yinan Zhao, Defu Zhi and Shubiao Zhang

Michela Pisani, Giovanna Mobbili and Paolo Bruni

**Cationic Lipids: Structure-Activity Relationships 349** 

**Physicochemical Aspects Governing the Process 381**  Manuel Alatorre-Meda, Eustolia Rodríguez-Velázquez

Chapter 15 **Non-Viral Gene Delivery Systems Based on Cholesterol** 

Hu-Lin Jiang, You-Kyoung Kim, Chong-Su Cho

**Possible Vehicles for Gene Therapy? 439** Ureporn Kedjarune-Leggat and Peter A. Leggat

Chapter 19 **Chitosan-DNA/siRNA Nanoparticles for Gene Therapy 455** Qin Shi, Marcio J. Tiera, Xiaoling Zhang, Kerong Dai, Mohamed Benderdour and Julio C. Fernandes

> **Systems for Pulmonary siRNA Application 481**  Andrea Beyerle, Thomas Kissel and Tobias Stoeger

Michael A. Maslov and Marina A. Zenkova

Chapter 16 **Polycation-Mediated Gene Delivery: The** 

Chapter 17 **Medical Polymer-Based Gene Therapy 417**

Chapter 18 **Chitosan and Its Modifications: Are They**

Chapter 20 **Toxicity of Polymeric-Based Non-Viral Vector** 

and Julio R. Rodríguez

and Myung-Haing Cho

Chapter 14 **Neutral Liposomes and DNA Transfection 319**

**Production of Functional Liposomes for Gene Therapy 267**  Julia E. Trevisan, Leide P. Cavalcanti, Cristiano L. P. Oliveira, Lucimara G. de La Torre and Maria Helena A. Santana

**and Gene Transfer Activity 243** Takehisa Dewa, Tomohiro Asai, Naoto Oku

and Mamoru Nango

Chapter 13 **Cationic Liposomes in Different** 


Preface

photomicrographs.

trials.

Gene therapy provides great promises for cancer treatment. Two essential components are absolutely necessary in current gene therapy: an effective therapeutic gene that can

This book aims to provide an up-to-date report in gene delivery research. With the multidisciplinary contribution in gene delivery, the book covers: (1) various gene delivery systems, like cationic lipids, cationic polymers and silica nanoparticles; (2) methods to enhance delivery, such as ultrasound and microbubble; (3) materials with

The book provides an introductory text for nonspecialists in gene delivery, and prepares readers to perform well-controlled experiments with appropriate controls. It illustrates ideas and models with more than 100 figures, including 64

Many specialists are not familiar with both drug delivery and the molecular biology of DNA vectors. In this book, molecular biologists will gain a basic knowledge of lipids, liposome, and other gene delivery vehicles and lipids, while drug delivery scientists

We acknowledge our contributors and section editors for generously sharing their expertise and scientific skills. We hope this book can help the researchers come up with new ideas and finally bring the gene therapy approaches to the clinical

> **Xu-bo Yuan** Tianjin University

> > China

will better understand DNA, molecular biology, and DNA manipulation.

be expressed at a target site, and an efficient and safe delivery system.

modification and multifunction for the tumor or tissue targeting.
