**4. Results and discussion**

Table 2 shows the conductivity and parts per million of EDTA solution (5x10-4 M), measured with the conductivity meter, and the four different solutions of textured soya extract prepared.


Table 2. Conductivity y ppm of EDTA and textured soya extract solutions

Table 3 shows the resulting conductivity after mixing each of the CuSO4 solutions with the EDTA solution and the four textured soy extract solutions. These results are the differences between the measurement of the mixture of the CuSO4 solution with the soy chelating solution and the pure extraction solution.


Table 3. Conductivity of EDTA solution and textured soya extract solutions with the prepared CuSO4 solutions

Figure 4 shows graphically the results of the Table 3.

From these results, we can conclude that is necessary to prepare the textured soya extract solution by using 2 or 3 grams of textured soy in 100 ml of water, heating it to boiling point for 10 minutes and separating out the fiber by filtration.

Table 4 contains the conductivity and ppm of the aqueous EDTA solution and the aqueous textured soya extract using 3 grams of textured soya in 100 ml of deionizer water we prepared.


Table 4. conductivity and ppm of EDTA and textured soy extract solutions.

extract solution and we measured the conductivity of each one, using a conductivity meter. All measurements were made at room temperature and at average room pressure, and pH 7.

Table 2 shows the conductivity and parts per million of EDTA solution (5x10-4 M), measured with the conductivity meter, and the four different solutions of textured soya extract

(µs) 459.1 454.7 836.6 1191 63.6 ppm 308.5 303 564.6 820.3 40.51

Table 3 shows the resulting conductivity after mixing each of the CuSO4 solutions with the EDTA solution and the four textured soy extract solutions. These results are the differences between the measurement of the mixture of the CuSO4 solution with the soy chelating

0.025 481.8 402 401.4 443 820 0.05 958.9 645.3 853.4 835 1350.4 0.1 2157.9 1857.3 1619.4 1685 2172.4 0.15 2569.9 2969.3 2479.4 2614 3057.4 0.2 3195.9 3503.3 2876.4 3340 3682.4 0.25 3407.9 4310.3 3968.4 3952 4200.4 0.3 3780.9 4836.3 4590.4 4631 4919.4

2 grams textured soya (µs)

Table 3. Conductivity of EDTA solution and textured soya extract solutions with the

From these results, we can conclude that is necessary to prepare the textured soya extract solution by using 2 or 3 grams of textured soy in 100 ml of water, heating it to boiling point

Table 4 contains the conductivity and ppm of the aqueous EDTA solution and the aqueous textured soya extract using 3 grams of textured soya in 100 ml of deionizer water we

> Conductivity (µs) 1414 150.3 ppm 981 67.06

Table 4. conductivity and ppm of EDTA and textured soy extract solutions.

3 grams textured soya

> 3 grams textured soya (µs)

Textured soya extract EDTA (5x10-4M)

5 grams textured soya

> 5 grams textured soya (µs)

EDTA (5x10-4 M)

> EDTA (µs)

2 grams textured soya

Table 2. Conductivity y ppm of EDTA and textured soya extract solutions

**4. Results and discussion** 

Conductivity

Concentration CuSO4 (M)

prepared CuSO4 solutions

prepared.

solution and the pure extraction solution.

1 gram textured soya

1 gram textured soya (µs)

Figure 4 shows graphically the results of the Table 3.

for 10 minutes and separating out the fiber by filtration.

prepared.

Fig. 4. Conductivity of the mixture of different textured soya extracts solutions with EDTA solution with the CuSO4 solutions .

Table 5 shows the results of the conductivity and ppm of five aqueous Pb2+ solution prepared dissolved 0.01, 0.03, 0.05, 0.07 and 0.1 grams of Pb(NO3)2 in 10 ml of deionizer water, each one. In a similar process were prepared five aqueous solutions EDTA-Fe3+ dissolved 0.01, 0.03, 0.05, 0.07 and 0.1 grams of Pb(NO3)2 in 10 ml of aqueous EDTA solution and in the same way were prepared five aqueous solutions of textured soya extract-Pb2+ dissolved 0.01, 0.03, 0.05, 0.07 and 0.1 grams of Pb(NO3)2 in 10 ml of textured soya extract.


Table 5. Conductivity and ppm of aqueous solutions: Pb2+ , EDTA- Pb2+ and textured soy extract-Pb2+.

The conductivity and ppm of the mixture of EDTA and textured soya extract with aqueous Pb2+ solution, shown in the Table 5, are a result of subtracting the conductivity or ppm of the mixtures and conductivity and ppm from the EDTA and texture soya extract with Pb(NO3)2.

Figure 5 is a graphic representation of Table 5 results.

Table 6 shows the results of the conductivity and ppm of five aqueous Fe3+ solution prepared dissolved 0.01, 0.03, 0.05, 0.07 and 0.1 grams of Fe(NO3)3 in 10 ml of deionizer water, each one. In a similar process were prepared five aqueous solutions EDTA-Fe3+ dissolved 0.01, 0.03, 0.05, 0.07 and 0.1 grams of Fe(NO3)3 in 10 ml of aqueous EDTA solution and in the same way were prepared five aqueous solutions of textured soya extract-Fe3+ dissolved 0.01, 0.03, 0.05, 0.07 and 0.1 grams of Fe(NO3)3 in 10 ml of textured soya extract.

Fig. 5. Conductivity of aqueous solutions: Pb2+, EDTA- Pb2+ and textured soya extract-Pb2+ .


Table 6. Conductivity and ppm of aqueous solutions: Fe3+ , EDTA- Fe3+ and textured soya extract-Fe3+.

The conductivity and ppm of the mixture of EDTA and textured soya extract with aqueous Fe3+ solution, shown in the Table 6, are a result of subtracting the conductivity or ppm of the mixtures and conductivity and ppm from the EDTA and texture soya extract with Fe(NO3)3. Figure 6 is a graphic representation of the Table 6 results.

Table 7 shows the results of the conductivity and ppm of five aqueous Cd2+ solution prepared dissolved 0.01, 0.03, 0.05, 0.07 and 0.1 grams of CdSO4 in 10 ml of deionizer water, each one. In a similar process were prepared five aqueous solutions EDTA-Cd2+ dissolved 0.01, 0.03, 0.05, 0.07 and 0.1 grams of CdSO4 in 10 ml of aqueous EDTA solution and in the same way were prepared five aqueous solutions of textured soya extract-Cd2+ dissolved 0.01, 0.03, 0.05, 0.07 and 0.1 grams of CdSO4 in 10 ml of textured soya extract.

and in the same way were prepared five aqueous solutions of textured soya extract-Fe3+ dissolved 0.01, 0.03, 0.05, 0.07 and 0.1 grams of Fe(NO3)3 in 10 ml of textured soya extract.

Lineal (soy + Pb2)

Lineal (solution Pb2+) Lineal (EDTA + Pb2+)

0 0.02 0.04 0.06 0.08 0.1 0.12

**grams of Pb(NO3)2**

Textured soya extract-Fe3+ Ppm (µs)

EDTA- Fe3+ (5x10-4M) ppm (µs)

Fig. 5. Conductivity of aqueous solutions: Pb2+, EDTA- Pb2+ and textured soya extract-Pb2+ .

0.01 932.64 1351.34 138 177 902.1 1286.7 0.03 2335.34 3159.34 995 1261 2097 2826.7 0.05 3910.34 5072.34 2785 3421 3573 4620.7 0.07 5078.34 6446.34 4431 5336 5008 6302 0.1 7329.34 8964.34 6065 7141 6900 8442

Table 6. Conductivity and ppm of aqueous solutions: Fe3+ , EDTA- Fe3+ and textured soya

The conductivity and ppm of the mixture of EDTA and textured soya extract with aqueous Fe3+ solution, shown in the Table 6, are a result of subtracting the conductivity or ppm of the mixtures and conductivity and ppm from the EDTA and texture soya extract with Fe(NO3)3.

Table 7 shows the results of the conductivity and ppm of five aqueous Cd2+ solution prepared dissolved 0.01, 0.03, 0.05, 0.07 and 0.1 grams of CdSO4 in 10 ml of deionizer water, each one. In a similar process were prepared five aqueous solutions EDTA-Cd2+ dissolved 0.01, 0.03, 0.05, 0.07 and 0.1 grams of CdSO4 in 10 ml of aqueous EDTA solution and in the same way were prepared five aqueous solutions of textured soya extract-Cd2+ dissolved

0.01, 0.03, 0.05, 0.07 and 0.1 grams of CdSO4 in 10 ml of textured soya extract.

0

Fe3+ aqueous solutions ppm (µs)

Figure 6 is a graphic representation of the Table 6 results.

1000

2000

3000

**Conductivity (microsiems)**

Grams Fe(NO3)3

extract-Fe3+.

4000

5000

6000

Fig. 6. Conductivity of the aqueous solution of: Fe3+, EDTA-Fe3+ and textured soya extract-Fe3+.


Table 7. Conductivity and ppm of aqueous solutions: Cd2+ , EDTA- Cd2+ and textured soya extract-Cd2+.

The conductivity and ppm of the mixture of EDTA and textured soya extract with aqueous Cd2+ solution, shown in the Table 7, are a result of subtracting the conductivity or ppm of the mixtures and conductivity and ppm from the EDTA and textured soya extract solutions with CdSO4.

Figure 7 is a graphic representation of Table 7 results. Table 8 shows the results of the conductivity and ppm of five aqueous Hg22+ solution prepared dissolved 0.01, 0.03, 0.05, 0.07 and 0.1 grams of HgCl2 in 10 ml of deionizer water, each one. In a similar process were prepared five aqueous solutions EDTA-Hg22+ dissolved 0.01, 0.03, 0.05, 0.07 and 0.1 grams of HgCl2 in 10 ml of aqueous EDTA solution and in the same way were prepared five textured soya extract-Hg22+ dissolved 0.01, 0.03, 0.05, 0.07 and 0.1 grams of HgCl2 in 10 ml of textured soya extract.

The conductivity and ppm of the mixture of EDTA and extract of soybeans with aqueous Hg2 2+ solution, shown in Table VII, is a result of subtracting the conductivity or ppm of the mixtures from the EDTA and textured soya extract with HgCl2.

Fig. 7. Conductivity of the aqueous solution of: Cd2+, EDTA-Cd2+ and textured soya extract-Cd2+.


Table 8. Conductivity and ppm of aqueous solutions: Hg2 2+ , EDTA- Hg2 2+ and textured soya extract-Hg22+.

Figure 8 is a graphic representation of Table 8 results.

Table 9 shows the results of the conductivity and ppm of five aqueous Ni2+ solution prepared dissolved 0.01, 0.03, 0.05, 0.07 and 0.1 grams of NiCl2 in 10 ml of deionizer water, each one. In a similar process were prepared five aqueous solutions EDTA-Ni2+ dissolved 0.01, 0.03, 0.05, 0.07 and 0.1 grams of NiCl2 in 10 ml of aqueous EDTA solution and in the same way were prepared five aqueous textured soya extract-Ni2+ dissolved 0.01, 0.03, 0.05, 0.07 and 0.1 grams of NiCl2 in 10 ml of textured soya extract.


Table 9. Conductivity and ppm of aqueous solutions: Ni2+ , EDTA- Ni2+ and textured soya extract-Ni2+.

Lineal (EDTA + Cd2+) Lineal (Solution Cd2+)

Lineal (Soy + Cd2+)

0 0.02 0.04 0.06 0.08 0.1 0.12 **grams of CdSO4**

> Textured soya extract-Hg22+ Ppm (µs)

EDTA-Hg22+ (5x10-4M) Ppm (µs)

> EDTA-Ni2+ (5x10-4M) ppm (µs)

Fig. 7. Conductivity of the aqueous solution of: Cd2+, EDTA-Cd2+ and textured soya extract-

0.01 25 39.41 46 5 113 171 0.03 26.92 42.28 58 74 107.2 163.7 0.05 33.82 53.24 78 94 100 151.7 0.07 37.56 59.09 79 100 107 160.7 0.1 48.58 76.74 100 134 113.7 173

Table 8. Conductivity and ppm of aqueous solutions: Hg22+ , EDTA- Hg22+ and textured

Table 9 shows the results of the conductivity and ppm of five aqueous Ni2+ solution prepared dissolved 0.01, 0.03, 0.05, 0.07 and 0.1 grams of NiCl2 in 10 ml of deionizer water, each one. In a similar process were prepared five aqueous solutions EDTA-Ni2+ dissolved 0.01, 0.03, 0.05, 0.07 and 0.1 grams of NiCl2 in 10 ml of aqueous EDTA solution and in the same way were prepared five aqueous textured soya extract-Ni2+ dissolved 0.01, 0.03, 0.05,

0.01 720.25 1052.34 210 273 450.5 716.7 0.03 1849.35 2553.34 1108 1404 997 1413.7 0.05 2677.35 3585.34 1845 2306 2653 3519.7 0.07 4093.35 5287.34 3225 3940 4088 5194.7 0.1 6069.35 7575.34 4658 5599 5607 7006.7

Table 9. Conductivity and ppm of aqueous solutions: Ni2+ , EDTA- Ni2+ and textured soya

Textured soya extract-Ni2+solution Ppm (µs)

> Hg22+ aqueous Solutions ppm (µs)

Figure 8 is a graphic representation of Table 8 results.

0.07 and 0.1 grams of NiCl2 in 10 ml of textured soya extract.

Ni2+ aqueous solution ppm (µs)

**Conductivity (microsiems)**

Cd2+.

Grams HgCl2

soya extract-Hg22+.

Grams NiCl2

extract-Ni2+.

Fig. 8. Conductivity of the aqueous solution of: Hg22+, EDTA-Hg2+ and textured soya extract-Hg2+.

The conductivity and ppm of the mixture of EDTA and textured soya extract with aqueous Ni2+ solution, shown in the Table VIII, is a result of subtracting the conductivity or ppm of the mixtures from the EDTA and textured soya extract with NiCl2. Figure 9 is a graphic representation of Table 9 results.

Fig. 9. Conductivity of the aqueous solution of: Ni2+, EDTA-Ni2+ and textured soya extract-Ni2+.

In Table 10 we can see the amount of metal ion sequestering for the textured soya extract in five different amounts of each salt: 0.01, 0.03, 0.05, 0.07 and 0.1 grams.


Table 10. Amount of metal ion sequestering using textured soya extract

Figure 10 is a graphic representation of Table 10 results.

Fig. 10. Amount of metal ion sequestering using textured soya extract.

In Table 11 we can see the amount of metal ion sequestering for the EDTA in five different amounts of each salt: 0.01, 0.03, 0.05, 0.07 and 0.1 grams.


Table 11. Amount of metal ion sequestering using EDTA

Figure 11 is a graphic representation of Table 11 results

From the results obtained in Table 10 and Table 11, we can say that the amount of metal ion chelating increases with the increase of the concentration but the amount of salt chelated with textured soya extract is considerable major in comparison to the EDTA. In the case of Hg22+ ions, the textured soya extract and EDTA is not effective as a chelating agent. Another

 0.01 grams 0.03 grams 0.05 grams 0.07 grams 0.1 grams Pb2+ 0.0076 0.008 0.019 0.018 0.027 Fe3+ 0.0087 0.017 0.016 0.0089 0.021 Cd2+ 0.0082 0.013 0.020 0.017 0.025 Hg2+ ----- ----- ----- ----- ----- Ni2+ 0.0075 0.012 0.018 0.014 0.027

Pb2+ Fe3+ Cd2+ Ni2+

0.01 0.03 0.05 0.07 0.1 **ions grams**

In Table 11 we can see the amount of metal ion sequestering for the EDTA in five different

 0.01 grams 0.03 grams 0.05 grams 0.07 grams 0.1 grams Pb2+ 0.0025 0.013 0.008 0.006 0.004 Fe3+ 0.0005 0.003 0.045 0.001 0.006 Cd2+ 0.0026 0.013 ---- ---- ---- Hg2+ ---- ---- ---- ---- ---- Ni2+ 0.0032 0.013 0.001 0.0085 0.008

From the results obtained in Table 10 and Table 11, we can say that the amount of metal ion chelating increases with the increase of the concentration but the amount of salt chelated with textured soya extract is considerable major in comparison to the EDTA. In the case of Hg22+ ions, the textured soya extract and EDTA is not effective as a chelating agent. Another

Table 10. Amount of metal ion sequestering using textured soya extract

Fig. 10. Amount of metal ion sequestering using textured soya extract.

amounts of each salt: 0.01, 0.03, 0.05, 0.07 and 0.1 grams.

Table 11. Amount of metal ion sequestering using EDTA Figure 11 is a graphic representation of Table 11 results

Figure 10 is a graphic representation of Table 10 results.

0

0.005

0.01

0.015

**amount of sequestering ion**

0.02

0.025

0.03

difference is that EDTA is effective as chelating agent of Cd2+ only with low concentrations (less to 0.04 g of CdSO4 in 10 ml of EDTA solution 5x10-4M) but without exception the textured soya extract is a good chelating agent.

Fig. 11. Amount of metal ion sequestering using EDTA

In the case of HgCl2 it is a weak electrolyte, the ionization is partial, as showing in the reaction 7.

$$\text{HgCl}\_2\text{(s)} = \text{HgCl}^\*\text{ (aq)} + \text{Cl}^\cdot\text{(aq)}\tag{7}$$

Maybe for this reason the EDTA and textured soya extract are not effective as chelating agents. But the textured soya extract is effective as chelating agent at low concentrations; nevertheless, the EDTA is not an effective chelating agent even in low concentrations.

From the results of Table 11, we are able to calculate the equilibrium constant for the textured soya extract, using the equation 6, and the results being shown in Tables 12.


Table 12. Equilibrium constant of metal ion sequestering


From the results of Table 11, we are able to calculate the equilibrium constant for the EDTA, using the equation 6, and the results being shown in Tables 13.

Table 13. Equilibrium constant of metal ion sequestering
