**2.1 Plant material and conduction of experiments**

In this study were used seeds from two different cultivars of soybean (*Glycine max* L.): Pintado, representative of the Brazilian Midwest region, characterized by the predominance of the Brazilian savanna (cerrado) features and BRS 133, representative of the South region, with features adapted to the soil and climate of this geography.

Three experiments were carried out in the Xenobiotic Lab from Department of Chemistry and Biochemistry, Institute of Biosciences, UNESP, Botucatu, in a germination chamber at 25°C in the dark.

Seeds were germinated on filter paper rolls moistened with distilled water or with different solutions. The volume of such solutions used in the treatments was 2.5 mL X g filter paper weight. The germination rolls were placed into plastic containers, each with a perforated lid. In the germination evaluations, seeds presenting root length equal to or greater than to 2 mm were considered germinated (Duran & Tortosa, 1985).

In the three experiments were adopted the experimental design completely randomized, with four replicates and twenty-five seeds per plot. The results were subjected to analysis of variance. The treatments were compared by Tukey test at 1% probability. The experiments were conducted in three phases.

#### **2.2 First experiment**

Seeds of two soybean cultivars were treated with the recommended level of Cruiser 350 FS - **D1** - (100 mL f.p./100Kg seed), with twice the recommended level of Cruiser 350 FS - **D2** - (200 mL f.p./100Kg seeds) and the control seeds were treated only with distilled water - **D0**. The counting of germinated seeds of the three treatments was performed at 24, 36, 48, 60 and 72 h of imbibition.

#### **2.3 Second experiment**

Seeds of two soybean cultivars were treated with the recommended level of Cruiser 350 FS - **D1** - (100 mL f.p./100Kg seed) and the control seeds were treated only with distilled water - **D0**.

#### **2.3.1 Presence of heavy metal – aluminum**

Followed by treatment with the levels D0 and D1 of Cruiser, germination paper leaves were moistened with solutions of aluminum sulphate at concentrations of 0; 5; 10 and 15 mmol L-1. Germination evaluations were performed at 24, 36, 48, 60 and 72 h of imbibition in the solutions of different concentrations of aluminum sulfate. At the end of the experiment (72 h) the embryo axis were removed and weighed.
