*2.2.6. Rheological test*

Small amplitude oscillatory shear was used to follow the gelation process of AX1 and AX2 solutions at 10% (w/v). Solutions were mixed with laccase (1.675 nkat per mg AX) and immediately placed on the parallel plate geometry (4.0 cm in diameter) of a strain controlled rheometer (Discovery HR-3 rheometer; TA Instruments, New Castle, DE, US). Exposed edges were covered with silicone oil to prevent evaporation. The dynamic rheological parameters used to evaluate the gel network were the storage modulus (G'), loss modulus (G"), crossover point (G'>G"), and tan delta (tan δ, G"/G'). AX1 and AX2 gelation were monitored at 0.25 Hz and 5% strain. At the end of the network formation a frequency sweep (0.01–10 Hz) was carried out. Rheological measurements were performed in duplicate [4].
