**4. Conclusion**

Polyphenolic polymers of rutin and esculin were synthesized using a laccase from *Trametes versicolor*. These polymers were fractioned by diafiltration process.

The analyses of rutin polymers by FTIR showed the presence of new C-C and C-O bonds and the desperation of a C-H bond on monomer. These results suggested that polyrutin were synthesized through phenylene and oxyphenylene units. For polyesculin fraction, FTIR analyses indicated the presence of only C-C bond.

Free radical scavenging activity of rutin was decreased by the enzymatic polymerization while polyesculin fractions showed a high antiradical activity compared to monomeric esculin. This behaviour suggested that the antioxidant activity depend on the position of linkage through the polymerization reaction. For esculin, it seemed that the polymerization didn't affect groups implicated in the antioxidant activity. This could explain the high antioxidant activity values observed for polyesculin.

Both polyrutin and polyesculin fractions exhibited a high XO inhibition activity, iron chelating and cupric reducing antioxidant capacities.
