**4. Sample collection**

Among the 1500 patients visiting the Department of Medicine, Faculty of Medicine, Benghazi University hospital for medical check up, 650 subjects were taken for the study. 200 subjects taken as controls(100 –males; 100-females) and 250 (100-males; 150-females) subjects formed the obese group and 200 formed the diabetes group(100 males and 100 females).Fasting blood samples were collected in vacutainer tubes with a gel separator and in heparinized tubes for HbA1C measurements and were centrifugated at 2000 rpm for 15 min at 4°C after an incubation period of 30 min. All biochemical variables were measured on the same day of the blood collection. Remaining serum specimens were stored at −20°C until analysis of adiponectin, leptin and resistin levels.
