**5. Biochemical methods**

Obesity is a major public health concern of Libya with higher prevalence of obesity related diseases hypertension, type 2 diabetes (T2DM) and coronary vascular disease.[7] Therefore, the present preliminary study was undertaken in Libyan subjects with their unique ethnicity, life style and cultural habits to demonstrate whether such differences occur in serum adipo‐ nectin, leptin and resistin levels in relation to adiposity particularly visceral adiposity. It was shown that BMI and Waist circumference could be taken as markers of subcutaneous obesity and abdominal obesity respectively. Along with these anthropometric measurements glycemic status, lipid profile were studied and correlated with adiponectin, leptin and resistin levels in

The subjects included in the study were local Libyan subjects, Benghazi. Informed consent was taken from each participant and approval for the study taken from Institute Ethics Review

Height, weight, waist circumference, were measured. Their age, duration of diabetes and medicine usage as well as personal and family history were noted.The measurements and

BMI was calculated by the formula: Weight (kg)/Height2 (m2). Normal BMI was taken as 18-25 kg/m2. Patients with BMI values of 25-30 kg/m2 were classified as overweight; and those with

Waist circumference (cm) was measured parallel to the midpoint between the lower limit of the 12th costa and the ischial spine. The limits were accepted as >102 cm in men, and >88 cm in

Among the 1500 patients visiting the Department of Medicine, Faculty of Medicine, Benghazi University hospital for medical check up, 650 subjects were taken for the study. 200 subjects taken as controls(100 –males; 100-females) and 250 (100-males; 150-females) subjects formed the obese group and 200 formed the diabetes group(100 males and 100 females).Fasting blood samples were collected in vacutainer tubes with a gel separator and in heparinized tubes for HbA1C measurements and were centrifugated at 2000 rpm for 15 min at 4°C after an incubation period of 30 min. All biochemical variables were measured on the same day of the blood collection. Remaining serum specimens were stored at −20°C until analysis of adiponectin,

obese and type 2 diabetes (T2DM) subjects.

Board(Faculty of Medicine, Benghazi University, Benghazi).

examinations were carried out by the same physician.

**2. Subjects taken for the study**

106 Treatment of Type 2 Diabetes

**3. Anthropometric measurements**

BMI ≥30 kg/m2 were considered obese.

**4. Sample collection**

leptin and resistin levels.

women (ATP: adult treatment panel III criteria).

Serum glucose, total cholesterol (TC), HDL-cholesterol (HDL-C), triglyceride (TG) were measured by an enzymatic colorimetric method.Serum adiponectin levels were measured by enzyme linked immunosorbent assay (ELISA) with a sensitivity of 3 ng/ml. Serum leptin levels were measured by an active human leptin ELISA (DSL, Diagnostic System Laboratories, USA) with a sensitivity of 0.05 ng/ml. Serum resistin levels were measured by ELISA (Linco Research, USA) with a sensitivity of 0.16 ng/ml.
