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**21** 

 *China* 

**A Preliminary In Vivo Study on the** 

*2Department of Macromolecule Science, Fudan University, Shanghai,* 

Lu Yan1, Zhao Xia1, Shao Zhengzhong2, Cao Zhengbing2 and Cai Lihui1 *1Department of Otorhinolaryngology, Huashan Hospital, Fudan University, Shanghai* 

Biomaterials used for tissue engineering should have the property of good histocompatibility, superb plasticity and desired degradability, so that it can be extensively applied for defect tissue repairing with excellent clinical outcome. In the past decade, silk fibroin has become one of the most favored biomaterials for its wide availability, superb performance and readiness to be shaped for different purposes in tissue engineering[1-14],. Porous scaffolds made by silk fibroin can be made into different pore size and porosity to serve for different needs of tissue repairing. The porous structure may contribute to the mass exchange in the scaffolds. However, the implanted protein scaffolds will degrade and can hardly be separated from host tissues. Therefore, little has been reported on

In this chapter, progress in study of silk fibroin scaffold in tissue engineering application and biocompatibility research will be introduced, then our histocompatibility experiment of porous scaffolds will be reported. In our experiment, porous scaffolds were made platy and buried in subcutaneous part of the back of SD rat. Tissue reaction was observed, and the

It is known to all that the application of silk production as un-absorb suture has many years of history. Along with the progress of tissue engineering techniques, scientists can extract natural polymeric materials- fibroin from silk and make it into different forms to fit various needs of tissue engineering[1]. Vitro studies show silk fibroin is biodegradable. The speed and degree of degradation can be adjusted through changing physicochemical property[2-4]. The product of degradation is mainly free amino acid and has no toxic side-

Silk fibroin can be easily made into different forms to serve for various needs such as membrane, gelatum, knitting scaffold, porous scaffold and electrospinning scaffold. Therefore, it has been gradually utilized in various medical field such as drug delivery[5,6], nerve regeneration[7-9], dermis healing[10], artificial ligament repair[11,12], bone or cartilage

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histocompatibility experiment in vivo for silk fibroin[15-22].

value of silk fibroin as tissue engineering scaffold material was discussed.

**2. The application of silk fibroin in tissue engineering** 

**1. Introduction** 

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**Histocompatibility of Silk Fibroin** 

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