**Peptide and Protein Neurotoxin Toolbox in Research on Nicotinic Acetylcholine Receptors**

Victor Tsetlin and Igor Kasheverov

Additional information is available at the end of the chapter

http://dx.doi.org/10.5772/58240

## **1. Introduction**

The chapter briefly covers the history of protein and peptide neurotoxins in research on nicotinic acetylcholine receptors (nAChR). It all started with a great help of α-bungarotoxin and other similar α-neurotoxins from snake venoms in isolation from the *Torpedo* ray electric organ of the muscle-type nAChR as a first individual membrane receptor. The next contribu‐ tion of α-neurotoxins was the discovery with their aid of the first neuronal nAChR in the brain now known as homooligomeric α7 nAChR. An overview of various α-neurotoxins (so-called three-finger toxins) is presented below showing the structural differences between them, as well as the benefits of their current application for identification and quantification of different nAChR subtypes at normal state and at various pathologies such as Alzheimer's and Parkin‐ son's diseases, psychiatric diseases and nicotine addiction. A special emphasis is placed on the work at our institute, starting with the first detection of nAChRs as targets for the so-called weak or "non-conventional" neurotoxins. Recently, in proteomic studies of snake venoms, novel structural types have been discovered, such as covalently connected dimeric α-cobra‐ toxin or, on the contrary - azemiopsin, the first peptide from venoms which does not contain disulfide bonds but still blocks selectively the muscle-type nAChR.

A generous source for sophisticated tools in research on nAChRs is combinatorial peptide libraries from the venoms of *Conus* marine snails. In particular, they contain α-conotoxins which not only distinguish muscle nAChRs from neuronal ones, but some of them block specifically distinct neuronal nAChR subtypes. At present, combinations of snake and snail toxins are widely used in fundamental research and in pharmacological studies.

The chapter briefly summarizes information on the spatial organization and subunit compo‐ sition of different nAChR subtypes, but considers in more detail important contributions of peptide and protein neurotoxins into elucidation of the topography of the nAChR binding

sites. The information mainly came from the X-ray structures of their complexes with the acetylcholine-binding protein (AChBP), an excellent structural model of the ligand-binding domain of nAChRs. These complexes are considered as initial blocks for design of novel drugs.

nAChRs. Neuronal heteromeric nAChRs are composed of two types of subunits: α and β. At present there are 9 types of neuronal α-subunits (α2-α10) and three types of β subunits (β2 β4); α and β subunits in the muscle-type receptors presumed to be α1 and β1 ones. The characteristic feature of α-subunit is a vicinal disulfide between two neighboring Cys residues in the binding site (Cys192-Cys193 in the amino-acid sequence of the *Torpedo* α-subunit) which is not present in β or other "non-α" subunits. In recent years it became clear that diverse nAChRs are very much widespread and play different functional roles also outside the neuromuscular junctions or central nervous system. In fact, neuronal nAChR subunits were found on the immune system cells, skin, lung tissue and other. The respective receptors got the name of "non-neuronal" nAChRs thus making a third group of nicotinic acetylcholine receptors (see

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**Figure 1.** Spatial organization of nAChRs. А – Schematic presentation of *Torpedo* nAChR, consisting of 5 subunits with the ion channel along the central axis. Two binding sites of agonists (acetylcholine and others) and competitive antag‐ onist (α-neurotoxins from snakes, α-conotoxins from *Conus* mollusks and others) are located at the interfaces of the α1/γ and α1/δ-subunits and marked with asterisks. В – Spatial organization of *Torpedo marmorata* nAChR derived from its cryo-electron microscopy structure. Subunits α1, β1, γ and δ are colored in red, green, cyanic and blue, respec‐ tively. Three main domains of the receptor – extracellular (ECD), transmembrane (TMD), consisting of 4 α-helical frag‐ ments (M1-M4), and intracellular (ICD) are shown. C – Schematic presentation of two representatives of neuronal nAChRs – homooligomeric and heterooligomeric ones. The probable binding sites of agonists and competitive antag‐

Structurally, the *Torpedo* nAChR is a prototype for all members of the nAChR family. First of all, it is a pentamer (composed of 5 subunits) as follows from the cryo-electron microscopy structure of the *Torpedo marmorata* receptor (see Figure 1, B). There are no structural data of this sort for any other nAChR, but their pentameric composition was presumed from computer

reviews [7,8]).

onist are marked with black circles.

## **2. Muscle-type, neuronal and "non-neuronal" nAChRs — Brief overview**

Before considering in detail protein and peptide neurotoxins on which the Chapter is mostly focused, it is reasonable to give very shortly the information about various types of nAChR which will make easier later discussions of the specificity of one or another toxin to a particular nAChR subtype.

As mentioned in the Introduction, α-bungarotoxin made possible identification and isolation in a pure form of the nAChR from the *Torpedo* ray electric organ. Later it was found that this receptor is composed of 5 subunits arranged around the central axis along which an ion channel should be arranged (Figure 1, A). The subunits in the order of their increasing molecular masses (estimated from the SDS-gel electrophoresis) have been named α, β, γ and δ. The molecular mass of the receptor complex is around 250 kD and it should contain two α subunits and by one of the "non- α" subunits. When nucleotide sequences of the *Torpedo* nAChR subunits and of those from mammalian muscles were established it became clear that those receptors are highly homologous. In fact, the mammalian embryonic nAChR has the same subunit stoichi‐ ometry (2α, β, γ and δ), but in the mature form it has an ε subunit instead of γ. Although the relevant information at present is available in numerous biochemistry books and reviews [1-4], it should be mentioned here that nAChRs are ligand-gated ion channels: binding of a ligand (acetylcholine, nicotine or other specific agonists) will result in the channel opening and passing sodium or calcium ions will activate a variety of signaling cascades. On the other hand, binding at the same sites of competitive antagonists such as α-bungarotoxin will prevent both binding of agonists and subsequent channel opening; some so-called non-competitive antagonists, like phencyclidine, bind directly to the channel moiety but they are not discussed here.

Earlier it was thought that the ligand-binding sites of nAChRs lie within the α-subunits, hence there should be two binding sites on the muscle-type nAChRs. To-day we know that, indeed, the main contributions to binding of agonists or competitive antagonists are donated by the α-subunits. Moreover, even isolated α-subunit and its fragment in the amino-acid region 170-200 can bind α-bungarotoxin, although with lower affinity than the whole-size receptors [5,6]. However, now it is well established that the binding sites are situated at the interfaces of the α-subunits with their neighbors, and it is the variability of functional groups brought to the binding sites by less conservative "non-alpha" subunits which underlies the differences in specificity between individual nAChR subtypes [4].

What are the types and subtypes of nicotinic acetylcholine receptors? As mentioned above, binding of radioactive α-bungarotoxin to brain membranes finally brought to life the nAChR presently known as homopentameric α7 nAChR that is composed of five identical α7-subunits. Thus, we have an example of homooligomeric receptor belonging to the family of neuronal nAChRs. Neuronal heteromeric nAChRs are composed of two types of subunits: α and β. At present there are 9 types of neuronal α-subunits (α2-α10) and three types of β subunits (β2 β4); α and β subunits in the muscle-type receptors presumed to be α1 and β1 ones. The characteristic feature of α-subunit is a vicinal disulfide between two neighboring Cys residues in the binding site (Cys192-Cys193 in the amino-acid sequence of the *Torpedo* α-subunit) which is not present in β or other "non-α" subunits. In recent years it became clear that diverse nAChRs are very much widespread and play different functional roles also outside the neuromuscular junctions or central nervous system. In fact, neuronal nAChR subunits were found on the immune system cells, skin, lung tissue and other. The respective receptors got the name of "non-neuronal" nAChRs thus making a third group of nicotinic acetylcholine receptors (see reviews [7,8]).

sites. The information mainly came from the X-ray structures of their complexes with the acetylcholine-binding protein (AChBP), an excellent structural model of the ligand-binding domain of nAChRs. These complexes are considered as initial blocks for design of novel drugs.

**2. Muscle-type, neuronal and "non-neuronal" nAChRs — Brief overview**

Before considering in detail protein and peptide neurotoxins on which the Chapter is mostly focused, it is reasonable to give very shortly the information about various types of nAChR which will make easier later discussions of the specificity of one or another toxin to a particular

As mentioned in the Introduction, α-bungarotoxin made possible identification and isolation in a pure form of the nAChR from the *Torpedo* ray electric organ. Later it was found that this receptor is composed of 5 subunits arranged around the central axis along which an ion channel should be arranged (Figure 1, A). The subunits in the order of their increasing molecular masses (estimated from the SDS-gel electrophoresis) have been named α, β, γ and δ. The molecular mass of the receptor complex is around 250 kD and it should contain two α subunits and by one of the "non- α" subunits. When nucleotide sequences of the *Torpedo* nAChR subunits and of those from mammalian muscles were established it became clear that those receptors are highly homologous. In fact, the mammalian embryonic nAChR has the same subunit stoichi‐ ometry (2α, β, γ and δ), but in the mature form it has an ε subunit instead of γ. Although the relevant information at present is available in numerous biochemistry books and reviews [1-4], it should be mentioned here that nAChRs are ligand-gated ion channels: binding of a ligand (acetylcholine, nicotine or other specific agonists) will result in the channel opening and passing sodium or calcium ions will activate a variety of signaling cascades. On the other hand, binding at the same sites of competitive antagonists such as α-bungarotoxin will prevent both binding of agonists and subsequent channel opening; some so-called non-competitive antagonists, like phencyclidine, bind directly to the channel moiety but they are not discussed

Earlier it was thought that the ligand-binding sites of nAChRs lie within the α-subunits, hence there should be two binding sites on the muscle-type nAChRs. To-day we know that, indeed, the main contributions to binding of agonists or competitive antagonists are donated by the α-subunits. Moreover, even isolated α-subunit and its fragment in the amino-acid region 170-200 can bind α-bungarotoxin, although with lower affinity than the whole-size receptors [5,6]. However, now it is well established that the binding sites are situated at the interfaces of the α-subunits with their neighbors, and it is the variability of functional groups brought to the binding sites by less conservative "non-alpha" subunits which underlies the differences in

What are the types and subtypes of nicotinic acetylcholine receptors? As mentioned above, binding of radioactive α-bungarotoxin to brain membranes finally brought to life the nAChR presently known as homopentameric α7 nAChR that is composed of five identical α7-subunits. Thus, we have an example of homooligomeric receptor belonging to the family of neuronal

specificity between individual nAChR subtypes [4].

nAChR subtype.

114 Neurochemistry

here.

**Figure 1.** Spatial organization of nAChRs. А – Schematic presentation of *Torpedo* nAChR, consisting of 5 subunits with the ion channel along the central axis. Two binding sites of agonists (acetylcholine and others) and competitive antag‐ onist (α-neurotoxins from snakes, α-conotoxins from *Conus* mollusks and others) are located at the interfaces of the α1/γ and α1/δ-subunits and marked with asterisks. В – Spatial organization of *Torpedo marmorata* nAChR derived from its cryo-electron microscopy structure. Subunits α1, β1, γ and δ are colored in red, green, cyanic and blue, respec‐ tively. Three main domains of the receptor – extracellular (ECD), transmembrane (TMD), consisting of 4 α-helical frag‐ ments (M1-M4), and intracellular (ICD) are shown. C – Schematic presentation of two representatives of neuronal nAChRs – homooligomeric and heterooligomeric ones. The probable binding sites of agonists and competitive antag‐ onist are marked with black circles.

Structurally, the *Torpedo* nAChR is a prototype for all members of the nAChR family. First of all, it is a pentamer (composed of 5 subunits) as follows from the cryo-electron microscopy structure of the *Torpedo marmorata* receptor (see Figure 1, B). There are no structural data of this sort for any other nAChR, but their pentameric composition was presumed from computer modeling and from some indirect data like electrophysiology analysis. At present there are no doubts that all nAChRs are indeed either pentameric homooligomers (made exclusively of 5 α-type subunits, like α7 nAChR, α9 nAChR or α9/α10 nAChR) or pentameric heterooligomers (composed of α and other subunits) (Figure 1, C) – for example, one of the best presented in the brain is α4β2 nAChR [9]. As already mentioned, all nAChRs should be built similarly to *Torpedo* nAChR: namely, four transmembrane fragments M1-M4 in each subunit, the most inner ones M2 fragments lining the channel, the N-terminal extracellular fragments of each subunit together forming the ligand-binding domain excellently imitated by the X-ray structure of the acetylcholine-binding protein (AChBP) (see below). The long intracellular loops between transmembrane fragments M3 and M4 of each subunit together form the cytoplasmic (intracellular) domain.

namely for erabutoxins a and b [18,19] (see Figure 2, A). The molecule has three loops, with a predominant β-structure, fixed in the space by 4 disulfide bridges forming a sort of a knot. This folding gave the name of "three-finger proteins" to α-neurotoxins. Later spatial structures have been determined both by NMR and X-ray crystallography for different short- and longchain α-neurotoxins, including α-bungarotoxin [20,21]. Long-chain α-neurotoxins have the same three -finger folding as the short ones, but contain a longer C-terminal tail and an additional 5th disulfide in the central loop II (Figure 2, B). In the structures of some long-chain α-neurotoxins (α-bungarotoxin, α-cobratoxin [22] or neurotoxin I from *Naja oxiana* [23]) a short

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**Figure 2.** Spatial structures of snake 'three-finger' toxins interacting with nAChRs. The 'fingers' are marked with Ro‐ man numbers; N-termini are labeled as well. A - erabutoxin a (PDB ID: 5EBX). B – α-bungarotoxin (1KFH); the 5th disul‐ fide bridge in loop II is colored in magenta (contrary to all other disulfides in orange) and α-helix at tip of this loop is colored intentionally in contrast green. C – κ-bungarotoxin (1KBA); 5th disulfide bridges in loops II are colored in red. D – haditoxin (3HH7). E – dimeric α-cobratoxin (4AEA), where disulfide bridges between Cys3 from one monomer and Cys20 from the second monomer stabilize the dimeric molecule; two monomers are shown in blue and magenta, re‐ spectively. F - irditoxin (2H7Z); 'non-conventional' disulfides in loops I are colored in red and disulfide bond between

One of the characteristic features of α-neurotoxins is the stability of their three-dimensional structure fixed by 4 or 5 disulfide bridges. This conclusion is supported by high similarity of spatial structures determined by NMR at different conditions (varying pH and temperatures) and by X-ray crystallography. This may be one of the crucial factors explaining high efficiency of α-neurotoxin interactions with their targets, nicotinic acetylcholine receptors. As will be

the monomers is shown in blue. G - candoxin (1JGK); disulfide in loop I is shown in red.

α-helical fragment was found at the tip of the loop II (see Figure 2, B).

The first and the most direct structural evidence for a common three-dimensional organization of all nAChRs came from the crystal structure of AChBP [10]. Today even more convincing are the recently solved high-resolution X-ray structures of the whole-size prokaryotic mem‐ brane proteins belonging to the same superfamily of Cys-loop ligand-gated ion channels as nAChRs [11-13]. These proteins, each composed of 5 identical subunits, do not have large cytoplasmic domains (which apparently made their crystallization much more simple than of nAChRs or other mammalian Cys-loop receptors), but in the transmembrane and ligandbinding domains they are surprisingly similar to *Torpedo* nAChR. Moreover, the same type of structure was found for a Cys-loop receptor from *Caenorhabditis elegans*[14]. Now, after having these major facts about nicotinic acetylcholine receptors, we can open our toolbox and have a closer look on protein and peptide neurotoxins.

## **3. Snake venom neurotoxins utilized in research on nAChRs — Primary and three-dimensional structure**

The word "toolbox" in the chapter title in the first place is related to the snake venom proteins, at least historically. It was the component of *Bungarus multicinctus* venom which was found to block very efficiently the muscle-type nAChRs and could be considered as a good marker of those receptors. The history of the discovery of such a tool, namely protein neurotoxin αbungarotoxin, is presented in a recent review [15]. There Prof. Chang shares his memories about this discovery (exactly 50 years ago!) which played such a crucial role in understanding the structure and function of both snake neurotoxins and of one of their targets, namely nAChRs. Soon after the discovery of α-bungarotoxin, similar proteins were found in other snakes, in particular in cobra venoms and the whole family got the name of α-neurotoxins (see reviews [15-17]).

#### **3.1. α-Neurotoxins**

There are two structural types of α-neurotoxins: short-chain α-neurotoxins (60-62 amino acid residues, 4 disulfide bridges) and long-chain ones (66-75 amino acid residues, 5 disulfide bonds). The first X-ray structures have been determined for the short-chain α-neurotoxins, namely for erabutoxins a and b [18,19] (see Figure 2, A). The molecule has three loops, with a predominant β-structure, fixed in the space by 4 disulfide bridges forming a sort of a knot. This folding gave the name of "three-finger proteins" to α-neurotoxins. Later spatial structures have been determined both by NMR and X-ray crystallography for different short- and longchain α-neurotoxins, including α-bungarotoxin [20,21]. Long-chain α-neurotoxins have the same three -finger folding as the short ones, but contain a longer C-terminal tail and an additional 5th disulfide in the central loop II (Figure 2, B). In the structures of some long-chain α-neurotoxins (α-bungarotoxin, α-cobratoxin [22] or neurotoxin I from *Naja oxiana* [23]) a short α-helical fragment was found at the tip of the loop II (see Figure 2, B).

modeling and from some indirect data like electrophysiology analysis. At present there are no doubts that all nAChRs are indeed either pentameric homooligomers (made exclusively of 5 α-type subunits, like α7 nAChR, α9 nAChR or α9/α10 nAChR) or pentameric heterooligomers (composed of α and other subunits) (Figure 1, C) – for example, one of the best presented in the brain is α4β2 nAChR [9]. As already mentioned, all nAChRs should be built similarly to *Torpedo* nAChR: namely, four transmembrane fragments M1-M4 in each subunit, the most inner ones M2 fragments lining the channel, the N-terminal extracellular fragments of each subunit together forming the ligand-binding domain excellently imitated by the X-ray structure of the acetylcholine-binding protein (AChBP) (see below). The long intracellular loops between transmembrane fragments M3 and M4 of each subunit together form the

The first and the most direct structural evidence for a common three-dimensional organization of all nAChRs came from the crystal structure of AChBP [10]. Today even more convincing are the recently solved high-resolution X-ray structures of the whole-size prokaryotic mem‐ brane proteins belonging to the same superfamily of Cys-loop ligand-gated ion channels as nAChRs [11-13]. These proteins, each composed of 5 identical subunits, do not have large cytoplasmic domains (which apparently made their crystallization much more simple than of nAChRs or other mammalian Cys-loop receptors), but in the transmembrane and ligandbinding domains they are surprisingly similar to *Torpedo* nAChR. Moreover, the same type of structure was found for a Cys-loop receptor from *Caenorhabditis elegans*[14]. Now, after having these major facts about nicotinic acetylcholine receptors, we can open our toolbox and have a

**3. Snake venom neurotoxins utilized in research on nAChRs — Primary**

The word "toolbox" in the chapter title in the first place is related to the snake venom proteins, at least historically. It was the component of *Bungarus multicinctus* venom which was found to block very efficiently the muscle-type nAChRs and could be considered as a good marker of those receptors. The history of the discovery of such a tool, namely protein neurotoxin αbungarotoxin, is presented in a recent review [15]. There Prof. Chang shares his memories about this discovery (exactly 50 years ago!) which played such a crucial role in understanding the structure and function of both snake neurotoxins and of one of their targets, namely nAChRs. Soon after the discovery of α-bungarotoxin, similar proteins were found in other snakes, in particular in cobra venoms and the whole family got the name of α-neurotoxins (see

There are two structural types of α-neurotoxins: short-chain α-neurotoxins (60-62 amino acid residues, 4 disulfide bridges) and long-chain ones (66-75 amino acid residues, 5 disulfide bonds). The first X-ray structures have been determined for the short-chain α-neurotoxins,

cytoplasmic (intracellular) domain.

116 Neurochemistry

closer look on protein and peptide neurotoxins.

**and three-dimensional structure**

reviews [15-17]).

**3.1. α-Neurotoxins**

**Figure 2.** Spatial structures of snake 'three-finger' toxins interacting with nAChRs. The 'fingers' are marked with Ro‐ man numbers; N-termini are labeled as well. A - erabutoxin a (PDB ID: 5EBX). B – α-bungarotoxin (1KFH); the 5th disul‐ fide bridge in loop II is colored in magenta (contrary to all other disulfides in orange) and α-helix at tip of this loop is colored intentionally in contrast green. C – κ-bungarotoxin (1KBA); 5th disulfide bridges in loops II are colored in red. D – haditoxin (3HH7). E – dimeric α-cobratoxin (4AEA), where disulfide bridges between Cys3 from one monomer and Cys20 from the second monomer stabilize the dimeric molecule; two monomers are shown in blue and magenta, re‐ spectively. F - irditoxin (2H7Z); 'non-conventional' disulfides in loops I are colored in red and disulfide bond between the monomers is shown in blue. G - candoxin (1JGK); disulfide in loop I is shown in red.

One of the characteristic features of α-neurotoxins is the stability of their three-dimensional structure fixed by 4 or 5 disulfide bridges. This conclusion is supported by high similarity of spatial structures determined by NMR at different conditions (varying pH and temperatures) and by X-ray crystallography. This may be one of the crucial factors explaining high efficiency of α-neurotoxin interactions with their targets, nicotinic acetylcholine receptors. As will be shown later, α-neurotoxins essentially preserve their conformation in complexes with the AChBP [24], with the ligand-binding domain of individual α1 subunit of nAChR [25] and with the chimera of AChBP and α7 nAChR extracellular domain [26].

translational modification found for the whole huge family of three-finger toxins. Dimeric αcobratoxin retained, although at a lower level, the capacity to block α7 and muscle-type nAChRs and in addition acquired the ability to block α3β2 nAChR - again, with lower potency than did κ-bungarotoxin [35]. Interestingly, selective reduction of the disulfides in the loop II of dimeric α-cobratoxin abolished its activity against α7 nAChR. It could be expected in view of earlier described similar modification of α-cobratoxin itself, but this chemical modification even increased the affinity for α3β2 nAChR [36]. Since dimeric α-cobratoxin is present in the *Naja kaouthia* cobra venom only in minute amounts (0.01% in crude venom, as compared to 10% for α-cobratoxin itself or to 0.1% for κ-bungarotoxin), unequivocal localization of inter‐ molecular disulfides by chemical means could not be done. Fortunately, dimeric α-cobratoxin has been recently crystallized (Figure 2, E) and the high-resolution X-ray structure revealed the disposition of the intermolecular disulfide bridges: the disulfide Cys3-Cys20 or Cys3'- Cys20' in each monomer is not formed, but Cys3 of one monomer finds Cys20' of another

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monomer, while Cys3' of the latter makes a disulfide with Cys20 of the former [36].

physiology experiments [36].

As will be shown later, the main contribution to binding of α-neurotoxins both to nAChRs and to their models comes from the tip of the central loop II of α-neurotoxins. In dimeric αcobratoxin the two tips are in close proximity and computer modeling showed impossibility of docking such a structure to AChBP, suggesting that some conformational changes should occur in the dimeric α-cobratoxin to ensure its binding observed in radioligand and electro‐

The discovery of dimeric α-cobratoxin was followed by finding another three-fingered toxin where monomers are connected by a disulfide bridge [37]. It was irditoxin isolated from Colubrid snake *Boiga irregularis*. In contrast to dimeric α-cobratoxin present in venom in minor amounts, irditoxin is a main component of boiga venom. Again, strictly speaking, irditoxin is neither a short- nor a long-chain α-neurotoxin: the monomers forming this toxin belong to nonconventional toxin type (see below) and each monomer contains an extra cysteine residue forming one disulfide bridge between two monomers (or protomers). None of these cysteines is present in classical α-neurotoxins. In the first protomer, the additional cysteine is located in loop I whereas in the second protomer it is in loop II. The three-dimensional structure of irditoxin [37] (see Figure 2, F) shows that the central loops II of the two protomers are oriented

A characteristic feature of this group of three-fingered toxins is the presence of additional disulfide bridge not in the central loop II, as in long-chain α-neurotoxins or in κ-bungarotoxins, but in the N- terminal loop I. Some representatives of this group were known long ago, but many of them did not have a strong toxicity (that is why their name was "weak toxins") and their targets were unknown. At present this group of toxins, consisting of 62-68 amino acid residues, is quite well investigated and has a more general name "non-conventional neuro‐ toxins" [38]. The toxicities for the most of group members are very low (5-80 mg/kg) in contrast to classical α-neurotoxin with toxicities in the range from 0.04 to 0.3 mg/kg. However, some very potent toxins (like γ-bungarotoxin with LD50 of 0.15 mg/kg) are also included in the

in a similar way as the central loops of dimeric α-cobratoxin (Figure 2, E).

**3.3. Weak (non-conventional) three-fingered neurotoxins**

#### **3.2. Dimeric three-finger neurotoxins**

First of all, we should mention here κ-bungarotoxins and several homologous neurotoxins which are dimers, but do not have covalent intermolecular bonds between monomers [27]. Each monomer is very similar to a typical long-chain α-neurotoxin: the same additional 5th disulfide at the tip of the central loop II, but a slightly shorter C-terminal tail (total number of amino acid residues 66 but not 75 as in α-bungarotoxin) (see Figure 2, C). The molecular targets of κ-bungarotoxins are neuronal nAChRs, but contrary to α-neurotoxins they have high affinity to neuronal α3β2 nAChR [28]. Interestingly, it was established about 20 years ago that there is one common property of α-neurotoxins and κ-neurotoxins, namely the additional disulfide in the loop II is essential for recognition of neuronal nAChRs. It was found that selective reduction of that disulfide and subsequent alkylation or removal of the respective cysteines in both types of toxins abolished their high affinity binding to α7 and α3β2 nAChRs, respectively (without decreasing the affinity of long-chain α-neurotoxins to muscle-type nAChRs [29,30]). On the other hand, introduction of additional disulfide into the central loop of short-chain α-neurotoxins considerably increased their affinity for α7 nAChR [31,32].

It is not yet absolutely clear why κ-bungarotoxins have preference for heteromeric nAChRs. There was a hypothesis that an important role in selectivity of κ-bungarotoxins towards α3β2 nAChRs belongs to the residue Lys26 [24]. However, its introduction to α-neurotoxin having a high affinity for α7 nAChRs only decreased considerably binding to this receptor but did not bring any affinity for α3β2 nAChRs [32]. Apparently, dimerization as such is important to force a protein, composed of two classical α-neurotoxins, to recognize a heteromeric neuronal nAChRs as can be seen on the example of other recently discovered dimeric neurotoxins.

One toxin, haditoxin from the King cobra venom [33] looks very similar to κ-bungarotoxin. Haditoxin is a non-covalent dimer composed of two short-chain α-neurotoxins, rather than of long-chain ones, and the monomers adopt a topological arrangement (Figure 2, D) reminiscent of that observed earlier for monomers in κ-bungarotoxin. Haditoxin can block not only muscletype nAChRs, as typically observed for short-chain α-neurotoxins, but surprisingly it also blocks homooligomeric α7 and heterooligomeric α3β2 nAChRs. This finding appears to be in contradiction with the earlier found necessity of the additional disulfide in the central loop for recognition of neuronal nAChRs. However, it should be kept in mind that blocking of neuronal nAChRs by haditoxin was observed only at very high toxin concentrations [33]. It should be also mentioned that, strictly speaking, haditoxin cannot be assigned to classical short-chain α-neurotoxins because its homology to erabutoxin is only 50%, whereas it is 75-80% with the muscarinic toxin-like proteins (MTLP) having different targets [34].

Novel types of dimeric α-neurotoxins were recently discovered: contrary to κ-bungarotoxin or haditoxin, these are covalently bound where two molecules of α-cobratoxin are connected by two intermolecular disulfide bonds [35]. Before describing a biological activity of this new tool, it should be mentioned that such intermolecular disulfide is the first case of this posttranslational modification found for the whole huge family of three-finger toxins. Dimeric αcobratoxin retained, although at a lower level, the capacity to block α7 and muscle-type nAChRs and in addition acquired the ability to block α3β2 nAChR - again, with lower potency than did κ-bungarotoxin [35]. Interestingly, selective reduction of the disulfides in the loop II of dimeric α-cobratoxin abolished its activity against α7 nAChR. It could be expected in view of earlier described similar modification of α-cobratoxin itself, but this chemical modification even increased the affinity for α3β2 nAChR [36]. Since dimeric α-cobratoxin is present in the *Naja kaouthia* cobra venom only in minute amounts (0.01% in crude venom, as compared to 10% for α-cobratoxin itself or to 0.1% for κ-bungarotoxin), unequivocal localization of inter‐ molecular disulfides by chemical means could not be done. Fortunately, dimeric α-cobratoxin has been recently crystallized (Figure 2, E) and the high-resolution X-ray structure revealed the disposition of the intermolecular disulfide bridges: the disulfide Cys3-Cys20 or Cys3'- Cys20' in each monomer is not formed, but Cys3 of one monomer finds Cys20' of another monomer, while Cys3' of the latter makes a disulfide with Cys20 of the former [36].

As will be shown later, the main contribution to binding of α-neurotoxins both to nAChRs and to their models comes from the tip of the central loop II of α-neurotoxins. In dimeric αcobratoxin the two tips are in close proximity and computer modeling showed impossibility of docking such a structure to AChBP, suggesting that some conformational changes should occur in the dimeric α-cobratoxin to ensure its binding observed in radioligand and electro‐ physiology experiments [36].

The discovery of dimeric α-cobratoxin was followed by finding another three-fingered toxin where monomers are connected by a disulfide bridge [37]. It was irditoxin isolated from Colubrid snake *Boiga irregularis*. In contrast to dimeric α-cobratoxin present in venom in minor amounts, irditoxin is a main component of boiga venom. Again, strictly speaking, irditoxin is neither a short- nor a long-chain α-neurotoxin: the monomers forming this toxin belong to nonconventional toxin type (see below) and each monomer contains an extra cysteine residue forming one disulfide bridge between two monomers (or protomers). None of these cysteines is present in classical α-neurotoxins. In the first protomer, the additional cysteine is located in loop I whereas in the second protomer it is in loop II. The three-dimensional structure of irditoxin [37] (see Figure 2, F) shows that the central loops II of the two protomers are oriented in a similar way as the central loops of dimeric α-cobratoxin (Figure 2, E).

#### **3.3. Weak (non-conventional) three-fingered neurotoxins**

shown later, α-neurotoxins essentially preserve their conformation in complexes with the AChBP [24], with the ligand-binding domain of individual α1 subunit of nAChR [25] and with

First of all, we should mention here κ-bungarotoxins and several homologous neurotoxins which are dimers, but do not have covalent intermolecular bonds between monomers [27]. Each monomer is very similar to a typical long-chain α-neurotoxin: the same additional 5th disulfide at the tip of the central loop II, but a slightly shorter C-terminal tail (total number of amino acid residues 66 but not 75 as in α-bungarotoxin) (see Figure 2, C). The molecular targets of κ-bungarotoxins are neuronal nAChRs, but contrary to α-neurotoxins they have high affinity to neuronal α3β2 nAChR [28]. Interestingly, it was established about 20 years ago that there is one common property of α-neurotoxins and κ-neurotoxins, namely the additional disulfide in the loop II is essential for recognition of neuronal nAChRs. It was found that selective reduction of that disulfide and subsequent alkylation or removal of the respective cysteines in both types of toxins abolished their high affinity binding to α7 and α3β2 nAChRs, respectively (without decreasing the affinity of long-chain α-neurotoxins to muscle-type nAChRs [29,30]). On the other hand, introduction of additional disulfide into the central loop of short-chain α-neurotoxins considerably increased their affinity for α7 nAChR [31,32].

It is not yet absolutely clear why κ-bungarotoxins have preference for heteromeric nAChRs. There was a hypothesis that an important role in selectivity of κ-bungarotoxins towards α3β2 nAChRs belongs to the residue Lys26 [24]. However, its introduction to α-neurotoxin having a high affinity for α7 nAChRs only decreased considerably binding to this receptor but did not bring any affinity for α3β2 nAChRs [32]. Apparently, dimerization as such is important to force a protein, composed of two classical α-neurotoxins, to recognize a heteromeric neuronal nAChRs as can be seen on the example of other recently discovered dimeric neurotoxins. One toxin, haditoxin from the King cobra venom [33] looks very similar to κ-bungarotoxin. Haditoxin is a non-covalent dimer composed of two short-chain α-neurotoxins, rather than of long-chain ones, and the monomers adopt a topological arrangement (Figure 2, D) reminiscent of that observed earlier for monomers in κ-bungarotoxin. Haditoxin can block not only muscletype nAChRs, as typically observed for short-chain α-neurotoxins, but surprisingly it also blocks homooligomeric α7 and heterooligomeric α3β2 nAChRs. This finding appears to be in contradiction with the earlier found necessity of the additional disulfide in the central loop for recognition of neuronal nAChRs. However, it should be kept in mind that blocking of neuronal nAChRs by haditoxin was observed only at very high toxin concentrations [33]. It should be also mentioned that, strictly speaking, haditoxin cannot be assigned to classical short-chain α-neurotoxins because its homology to erabutoxin is only 50%, whereas it is 75-80% with the

Novel types of dimeric α-neurotoxins were recently discovered: contrary to κ-bungarotoxin or haditoxin, these are covalently bound where two molecules of α-cobratoxin are connected by two intermolecular disulfide bonds [35]. Before describing a biological activity of this new tool, it should be mentioned that such intermolecular disulfide is the first case of this post-

the chimera of AChBP and α7 nAChR extracellular domain [26].

muscarinic toxin-like proteins (MTLP) having different targets [34].

**3.2. Dimeric three-finger neurotoxins**

118 Neurochemistry

A characteristic feature of this group of three-fingered toxins is the presence of additional disulfide bridge not in the central loop II, as in long-chain α-neurotoxins or in κ-bungarotoxins, but in the N- terminal loop I. Some representatives of this group were known long ago, but many of them did not have a strong toxicity (that is why their name was "weak toxins") and their targets were unknown. At present this group of toxins, consisting of 62-68 amino acid residues, is quite well investigated and has a more general name "non-conventional neuro‐ toxins" [38]. The toxicities for the most of group members are very low (5-80 mg/kg) in contrast to classical α-neurotoxin with toxicities in the range from 0.04 to 0.3 mg/kg. However, some very potent toxins (like γ-bungarotoxin with LD50 of 0.15 mg/kg) are also included in the group of non-conventional toxins. Since, as mentioned above, molecular targets of weak (nonconventional) toxins for a long time were unknown, an important step in this field was the work [39] where was discovered that weak toxin (WTX) from *Naja kaouthia* cobra venom interacted with micromolar affinity with the α7 and muscle-type nAChRs, the binding being practically irreversible. Later it was found [40] that candoxin (Figure 2, G), another nonconventional toxin, interacted both with α7 and muscle-type nAChR with high affinity. An interesting feature of candoxin is that its attachment to the muscle-type receptor was easily reversible. Even more impressing species specificity was reported for denmotoxin, a nonconventional toxin isolated from Colubrid snake *Boiga dendrophila*: it was able to interact irreversibly and with high affinity with chick muscle nAChR, but only with low affinity with mouse receptors [41].

in complex with acetylcholinesterases were the first examples presenting a three-finger toxin

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One of the most well-represented groups in the snake venoms are so-called cytotoxins (some of them were earlier called cardiotoxins) which apparently do not have a single well-defined target but disrupt the cell membranes thus inducing a multitude of effects (see reviews [51,52]). As a result of proteomic studies new three-finger proteins are being found in the snake venoms, and one of the minor components in the *Naja kaouthia* cobra venom was identified as a glycosylated cytotoxin I [53]. This post-translational modification, for the first time discovered for the family of three-finger toxins, considerably decreased the cytotoxicity of this protein, whereas enzymatic deglycosylation restored it to the level of cytotoxin I activity [53]. Another really a minor component of that venom (less than 0.01% in the crude venom) was a dimer of cytotoxin and α-cobratoxin connected by two intermolecular disulfide bridges which revealed

We also would like to mention here the recent discovery of three-finger neurotoxins which interact with another group of GPCR, namely with the adrenoreceptors [54,55]. These toxins are most similar to muscarinic toxins and were also isolated from the eastern green mamba *Dendroaspis angusticeps.* One such toxin (ρ-Da1a) has a very high affinity (0.35 nM) for the α1 adrenoreceptor, while another one (ρ-Da1b) has a lower affinity but is more selective towards α2 types [55]. Interestingly, these toxins are considered as possible drugs against prostate

Although it is not the topic of the present review, it is appropriate to mention here that there are three-finger proteins in nervous and immune system of mammals and insects belonging to the Ly6 family and some of them bind to nicotinic acetylcholine receptors and regulate their

Such peptides are not as numerous as α-neurotoxins or non-conventional toxins targeting different subtypes of nAChR. Until recently the only group was that of waglerins isolated from the venom of South Asian snake *Tropidolaemus wagleri* which consist of 22-24 amino acids and contain one disulfide bridge [64,65]. These toxins bind with high affinity to muscle-type nAChR [66]. Interestingly, waglerins can distinguish embryonic (α12β1 γδ) and "mature" (α12β1εδ) muscle-type nAChR: waglerin-1 efficiently blocks the ε-containing form, but not the γ-form of this receptor [67]. While snake venom α-neurotoxins bind with practically equal efficiency to the two binding sites (formed by two α-subunits with their non-α neighbors) in the muscle-type nAChRs, waglerin-1 binds 2100-fold more tightly to the α-ε than to the α-δ binding site of the mouse nAChR [68]. Several amino acid residues in the nAChR subunits participating in waglerin binding were identified by site directed mutagenesis [69], namely Asp59 and Asp173 were shown to be important for waglerin binding at both sites. On the other hand, the disulfide in waglerin was found to be essential for its activity, as well as several

functioning *in vivo* (see [56-59] and recent publications from our institute [60-63]).

**3.5. Peptides from snake venoms acting on nicotinic acetylcholine receptors**

residues in its N-terminal part of the amino acid sequence [70].

a weak activity against neuronal nicotinic acetylcholine receptors [35].

bound to its biological target [49,50].

hypertrophy.

#### **3.4. Three-finger snake neurotoxins having other targets than nicotinic acetylcholine receptors**

Before considering in detail the mechanisms of interactions between α-neurotoxins and nAChRs and describing their earlier and current roles of tools, it is appropriate to say a few words about the whole family of three-finger proteins from snake venoms (see reviews [16,17]). They all have the same "three-finger" fold but are decorated with quite different functionally active amino-acid residue and, as a result, attack distinct targets. For example, in the preceding paragraph we considered WTX from *Naja kaouthia* venom which blocked nicotinic acetylcho‐ line receptors. Its very low toxicity allowed testing of its behavioural activity on rats which suggested action on muscarinic acetylcholine receptors [42]. Indeed, subsequent radioligand analyses revealed the WTX interaction with the different subtypes of muscarinic acetylcholine receptors [43]. It should be noted here that we have a dualism of action for this group of the three-finger proteins from snake venom: namely, blocking of one acetylcholine receptor (the nicotinic one) belonging to the family of ligand-gated ion channels and another acetylcholine receptor, the muscarinic one which is a member of the superfamily of G-protein-coupled receptors (GPCR).

Much more strong effects on muscarinic acetylcholine receptors exert so-called muscarinic neurotoxins isolated from the green mamba *Dendroaspis angusticeps* [44-46]. Structurally these proteins are of the same type as short-chain α-neurotoxins. Interestingly, they can distinguish different subtypes (M1-M7) of muscarinic acetylcholine receptors and on some of them exert not the inhibitory, but the potentiating effects. There is not yet much information about how muscarinic toxins recognize their targets. A large series of mutations was performed both on the muscarinic toxin MT7 and on the M1 muscarinic receptor and the results of this pair-wise mutagenesis, analyzed by computer modelling, indicated that all three loops I-III should be involved in the interaction and the main binding site for this allosteric modulator is located in the extracellular loops of the receptor [46].

There are also several three-finger proteins from snake venoms (calciceptin, FS2) blocking Ca2+ channels [47,48]. We should also mention here fasciculin, a three-finger protein with 4 disulfides, targeting the acetylcholinesterase. Interestingly, the X-ray structures of fasciculin in complex with acetylcholinesterases were the first examples presenting a three-finger toxin bound to its biological target [49,50].

group of non-conventional toxins. Since, as mentioned above, molecular targets of weak (nonconventional) toxins for a long time were unknown, an important step in this field was the work [39] where was discovered that weak toxin (WTX) from *Naja kaouthia* cobra venom interacted with micromolar affinity with the α7 and muscle-type nAChRs, the binding being practically irreversible. Later it was found [40] that candoxin (Figure 2, G), another nonconventional toxin, interacted both with α7 and muscle-type nAChR with high affinity. An interesting feature of candoxin is that its attachment to the muscle-type receptor was easily reversible. Even more impressing species specificity was reported for denmotoxin, a nonconventional toxin isolated from Colubrid snake *Boiga dendrophila*: it was able to interact irreversibly and with high affinity with chick muscle nAChR, but only with low affinity with

**3.4. Three-finger snake neurotoxins having other targets than nicotinic acetylcholine**

Before considering in detail the mechanisms of interactions between α-neurotoxins and nAChRs and describing their earlier and current roles of tools, it is appropriate to say a few words about the whole family of three-finger proteins from snake venoms (see reviews [16,17]). They all have the same "three-finger" fold but are decorated with quite different functionally active amino-acid residue and, as a result, attack distinct targets. For example, in the preceding paragraph we considered WTX from *Naja kaouthia* venom which blocked nicotinic acetylcho‐ line receptors. Its very low toxicity allowed testing of its behavioural activity on rats which suggested action on muscarinic acetylcholine receptors [42]. Indeed, subsequent radioligand analyses revealed the WTX interaction with the different subtypes of muscarinic acetylcholine receptors [43]. It should be noted here that we have a dualism of action for this group of the three-finger proteins from snake venom: namely, blocking of one acetylcholine receptor (the nicotinic one) belonging to the family of ligand-gated ion channels and another acetylcholine receptor, the muscarinic one which is a member of the superfamily of G-protein-coupled

Much more strong effects on muscarinic acetylcholine receptors exert so-called muscarinic neurotoxins isolated from the green mamba *Dendroaspis angusticeps* [44-46]. Structurally these proteins are of the same type as short-chain α-neurotoxins. Interestingly, they can distinguish different subtypes (M1-M7) of muscarinic acetylcholine receptors and on some of them exert not the inhibitory, but the potentiating effects. There is not yet much information about how muscarinic toxins recognize their targets. A large series of mutations was performed both on the muscarinic toxin MT7 and on the M1 muscarinic receptor and the results of this pair-wise mutagenesis, analyzed by computer modelling, indicated that all three loops I-III should be involved in the interaction and the main binding site for this allosteric modulator is located in

There are also several three-finger proteins from snake venoms (calciceptin, FS2) blocking Ca2+ channels [47,48]. We should also mention here fasciculin, a three-finger protein with 4 disulfides, targeting the acetylcholinesterase. Interestingly, the X-ray structures of fasciculin

mouse receptors [41].

receptors (GPCR).

the extracellular loops of the receptor [46].

**receptors**

120 Neurochemistry

One of the most well-represented groups in the snake venoms are so-called cytotoxins (some of them were earlier called cardiotoxins) which apparently do not have a single well-defined target but disrupt the cell membranes thus inducing a multitude of effects (see reviews [51,52]). As a result of proteomic studies new three-finger proteins are being found in the snake venoms, and one of the minor components in the *Naja kaouthia* cobra venom was identified as a glycosylated cytotoxin I [53]. This post-translational modification, for the first time discovered for the family of three-finger toxins, considerably decreased the cytotoxicity of this protein, whereas enzymatic deglycosylation restored it to the level of cytotoxin I activity [53]. Another really a minor component of that venom (less than 0.01% in the crude venom) was a dimer of cytotoxin and α-cobratoxin connected by two intermolecular disulfide bridges which revealed a weak activity against neuronal nicotinic acetylcholine receptors [35].

We also would like to mention here the recent discovery of three-finger neurotoxins which interact with another group of GPCR, namely with the adrenoreceptors [54,55]. These toxins are most similar to muscarinic toxins and were also isolated from the eastern green mamba *Dendroaspis angusticeps.* One such toxin (ρ-Da1a) has a very high affinity (0.35 nM) for the α1 adrenoreceptor, while another one (ρ-Da1b) has a lower affinity but is more selective towards α2 types [55]. Interestingly, these toxins are considered as possible drugs against prostate hypertrophy.

Although it is not the topic of the present review, it is appropriate to mention here that there are three-finger proteins in nervous and immune system of mammals and insects belonging to the Ly6 family and some of them bind to nicotinic acetylcholine receptors and regulate their functioning *in vivo* (see [56-59] and recent publications from our institute [60-63]).

#### **3.5. Peptides from snake venoms acting on nicotinic acetylcholine receptors**

Such peptides are not as numerous as α-neurotoxins or non-conventional toxins targeting different subtypes of nAChR. Until recently the only group was that of waglerins isolated from the venom of South Asian snake *Tropidolaemus wagleri* which consist of 22-24 amino acids and contain one disulfide bridge [64,65]. These toxins bind with high affinity to muscle-type nAChR [66]. Interestingly, waglerins can distinguish embryonic (α12β1 γδ) and "mature" (α12β1εδ) muscle-type nAChR: waglerin-1 efficiently blocks the ε-containing form, but not the γ-form of this receptor [67]. While snake venom α-neurotoxins bind with practically equal efficiency to the two binding sites (formed by two α-subunits with their non-α neighbors) in the muscle-type nAChRs, waglerin-1 binds 2100-fold more tightly to the α-ε than to the α-δ binding site of the mouse nAChR [68]. Several amino acid residues in the nAChR subunits participating in waglerin binding were identified by site directed mutagenesis [69], namely Asp59 and Asp173 were shown to be important for waglerin binding at both sites. On the other hand, the disulfide in waglerin was found to be essential for its activity, as well as several residues in its N-terminal part of the amino acid sequence [70].

A new peptide was recently found in the snake venom possessing a capacity to block muscletype nAChR [71]. It is azemiopsin, isolated from the *Azemiops feae* viper venom, which consists of 21 amino acid residues. By the chain length azemiopsin is similar to waglerins and, moreover, shares with them a homologous C-terminal fragment. However, it possesses a unique structural feature: contrary to all earlier known proteins and peptides from the venoms of snakes or poisonous *Conus* mollusks (see below), whose structure is fixed by one or several S–S-bonds, azemiopsin contains no disulfides. It dose-dependently blocked acetylcholineinduced currents in *Xenopus* oocytes heterologously expressing human muscle nAChR, and was more potent against the adult (α12β1εδ) than the fetal (α12β1γδ) form. Ala-scanning and analysis of competition with α-bungarotoxin for binding to *Torpedo* nAChR resulted in identification of the azemiopsin residues essential for its activity which in general were found to be different from those responsible for the waglerin activity [71].

acting on nAChRs (ψ-, αA-, αAS-, αC-, αS- and αD), but they are not numerous, are not as

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widely used as α-conotoxins and will not be considered here.

3/5 α-conotoxins

4/3 α- conotoxins

4/4 α- conotoxins

4/6 α- conotoxins

4/7 α- conotoxins

identified in cDNA libraries.

acid residues between the C<sup>2</sup>

two or more nAChR subtypes (see Table).

**Toxin** *Conus species* **Amino acid sequence 1 Selectivity**

GI *C. geographus* ECCNPACGRHYSC\* α1β1γ/εδ MI *C. magus* GRCCHPACGKNYSC\* α1β1γ/εδ SIA *C. striatus* YCCHPACGKNFDC\* α1β1γ/εδ

*RgIA C. regius* GCCSDPRCRYRCR α9α10

AuIB *C. aulicus* GCCSYPPCFATNPDC\* α3β4

PnIA *C. pennaceus* GCCSLPPCAANNPDYC\* α3β2 PnIВ *C. pennaceus* GCCSLPPCALSNPDYC\* α7; α3β4

TxIA *C. textile* GCCSRPPCIANNPDLC\* α3β2

1 Scheme of disulfide closing for naturally-occurring α-conotoxins –

**Table 1.** Most studied members of naturally-occurring α-conotoxins.

–C3

and C<sup>3</sup>

–C4

ImI *C. imperialis* GCCSDPRCAWRC\* α7, α9α10; α3β2; α3β4

*BuIA C. bullatus* GCCSTPPCAVLYC\* α3(α6)β2, α3(α6)β4

MII *C. magus* GCCSNPVCHLEHSNLC\* α3β2(β3); α6-containing *Vc1.1 C. victoriae* GCCSDPRCNYDHPEIC\* α9α10; α3β4, α3(α5)β2

*ArIB* C. *arenatus* DECCSNPACRVNNPHVCRRR α7, α6α3β2β3, α3β2

\* indicates an amidated C-terminus; the names of α-conotoxins typed in italics mean that their structures were

α-Conotoxins are structurally subdivided into subgroups depending on the number of amino

loops, respectively. This structural feature affects the α-conotoxin specificity to particular nAChR subtypes. All at present known 3/5 α-conotoxins are potent blockers of muscle type nAChRs (and conventionally can be called 'muscle' α-conotoxins). The members of other subgroups (4/3, 4/4, 4/6, 4/7) act on various neuronal nAChR subtypes (and can be called 'neuronal' α-conotoxins). It is very rare when naturally occurring neuronal α-conotoxin blocks specifically only one neuronal nAChR subtype, usually neuronal α-conotoxins interact with

cysteines (see Table) forming the first and second

## **4. α-Conotoxins, peptides from poisonous marine snails** *Conus***, acting on nicotinic acetylcholine receptors**

Historically, snake venom α-neurotoxins were the first extremely important tools which made possible "digging out" in a purified form the first representative of the nAChR family, namely the muscle-type receptor from the *Torpedo* ray electric organ. Then, in the early 80s , the peptide toxins were discovered in the marine mollusk *Conus geographus* venom which caused postsy‐ naptic inhibition at the neuromuscular junction in frog and got the name of conotoxins [72]. The following studies brought to life a tremendous number of so-called conotoxins or cono‐ peptides from different species of *Conus* snails. The number of *Conus* species living in different seas and oceans is about 1000 and the available data show that the venom of each species should contain in excess of 1000 conopeptides. Thus, *Conus* mollusks provide researchers with huge combinatorial libraries of peptides. The main task of slowly moving *Conus* mollusks is to immobilize their preys (small fishes, worms etc.), that is why their venoms contain a variety of peptides paralyzing the nervous systems of their targets. Evolutionary each *Conus* species is adjusted to a particular area and a distinct food source, hence the individuality of each venom. There are several types of conotoxins differing in their targets: α-conotoxins block nAChRs, μ-conotoxins are acting on Na+ -channels, κ-conotoxins interact with K+ -channels, ωconotoxins block specifically certain Ca2+-channels and one of such ω-conotoxins became a very potent analgesic (trade name Ziconotide or Prialt; see more about these and many other conotoxins and conopeptides in recent reviews [73-75]). The number of discovered conotoxins is rapidly increasing because nowadays they appear not so much due to isolation from *Conus* venoms (usually available only in minute amounts) but due to deciphering mRNAs obtained from the venom glands.

Since this chapter is devoted to neurotoxic proteins and peptides interacting with nicotinic acetylcholine receptors, below we will consider only those conotoxins which target these receptors. The major group is α-conotoxins, competitive antagonists of nAChRs. They have 12-19 amino-acid residues, as a rule amidated C-terminus and two disulfide bonds between Cys residues C1 –C3 and C2 –C4 (see Table). There are also several other groups of conotoxins



1 Scheme of disulfide closing for naturally-occurring α-conotoxins –

A new peptide was recently found in the snake venom possessing a capacity to block muscletype nAChR [71]. It is azemiopsin, isolated from the *Azemiops feae* viper venom, which consists of 21 amino acid residues. By the chain length azemiopsin is similar to waglerins and, moreover, shares with them a homologous C-terminal fragment. However, it possesses a unique structural feature: contrary to all earlier known proteins and peptides from the venoms of snakes or poisonous *Conus* mollusks (see below), whose structure is fixed by one or several S–S-bonds, azemiopsin contains no disulfides. It dose-dependently blocked acetylcholineinduced currents in *Xenopus* oocytes heterologously expressing human muscle nAChR, and was more potent against the adult (α12β1εδ) than the fetal (α12β1γδ) form. Ala-scanning and analysis of competition with α-bungarotoxin for binding to *Torpedo* nAChR resulted in identification of the azemiopsin residues essential for its activity which in general were found

**4. α-Conotoxins, peptides from poisonous marine snails** *Conus***, acting on**

Historically, snake venom α-neurotoxins were the first extremely important tools which made possible "digging out" in a purified form the first representative of the nAChR family, namely

toxins were discovered in the marine mollusk *Conus geographus* venom which caused postsy‐ naptic inhibition at the neuromuscular junction in frog and got the name of conotoxins [72]. The following studies brought to life a tremendous number of so-called conotoxins or cono‐ peptides from different species of *Conus* snails. The number of *Conus* species living in different seas and oceans is about 1000 and the available data show that the venom of each species should contain in excess of 1000 conopeptides. Thus, *Conus* mollusks provide researchers with huge combinatorial libraries of peptides. The main task of slowly moving *Conus* mollusks is to immobilize their preys (small fishes, worms etc.), that is why their venoms contain a variety of peptides paralyzing the nervous systems of their targets. Evolutionary each *Conus* species is adjusted to a particular area and a distinct food source, hence the individuality of each venom. There are several types of conotoxins differing in their targets: α-conotoxins block

conotoxins block specifically certain Ca2+-channels and one of such ω-conotoxins became a very potent analgesic (trade name Ziconotide or Prialt; see more about these and many other conotoxins and conopeptides in recent reviews [73-75]). The number of discovered conotoxins is rapidly increasing because nowadays they appear not so much due to isolation from *Conus* venoms (usually available only in minute amounts) but due to deciphering mRNAs obtained

Since this chapter is devoted to neurotoxic proteins and peptides interacting with nicotinic acetylcholine receptors, below we will consider only those conotoxins which target these receptors. The major group is α-conotoxins, competitive antagonists of nAChRs. They have 12-19 amino-acid residues, as a rule amidated C-terminus and two disulfide bonds between


(see Table). There are also several other groups of conotoxins

, the peptide


the muscle-type receptor from the *Torpedo* ray electric organ. Then, in the early 80s

to be different from those responsible for the waglerin activity [71].

**nicotinic acetylcholine receptors**

122 Neurochemistry

nAChRs, μ-conotoxins are acting on Na+

from the venom glands.

–C3

and C2

–C4

Cys residues C1


\* indicates an amidated C-terminus; the names of α-conotoxins typed in italics mean that their structures were identified in cDNA libraries.

**Table 1.** Most studied members of naturally-occurring α-conotoxins.

α-Conotoxins are structurally subdivided into subgroups depending on the number of amino acid residues between the C<sup>2</sup> –C3 and C<sup>3</sup> –C4 cysteines (see Table) forming the first and second loops, respectively. This structural feature affects the α-conotoxin specificity to particular nAChR subtypes. All at present known 3/5 α-conotoxins are potent blockers of muscle type nAChRs (and conventionally can be called 'muscle' α-conotoxins). The members of other subgroups (4/3, 4/4, 4/6, 4/7) act on various neuronal nAChR subtypes (and can be called 'neuronal' α-conotoxins). It is very rare when naturally occurring neuronal α-conotoxin blocks specifically only one neuronal nAChR subtype, usually neuronal α-conotoxins interact with two or more nAChR subtypes (see Table).

Most of muscle 3/5 α-conotoxins can discriminate species-specifically two binding sites on muscle or *Torpedo* nAChRs. For example, α-conotoxins MI, GI or SIA have up to10000 times higher affinity for α1/δ- over α1/γ site in muscle nAChR [76,77]; in contrast to more effective binding of these peptides, although not with such a great difference, to α1/γ site in *Torpedo* receptor [78,79].

AChBP crystal structure revealed that such residues are all clustered in the middle of AChBP, at the interfaces between its subunits (or protomers). At present, the X-ray structures of several molluscan AChBPs are known (from *Lymnaea stagnalis*, *Aplysia californica*, *Bulinus truncatus*), as well of their complexes with a wide variety of agonists and antagonists which gave quite a detailed picture of the respective binding sites in these AChBPs and of their contacts with ligands. Biochemical data and computer modeling show convincingly that these structures shed light on the receptor binding sites *per se* and on the ligand disposition in the binding sites of muscle and neuronal nAChRs. The relevant information can be found in recent original papers [94-97] and reviews [98,99], and below we will consider in detail only the crystal

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Interestingly, the first AChBP crystal structure in complex with a competitive antagonist was that of *Lymanaea stagnalis* AChBP with bound α-cobratoxin [24]. (In parentheses it may be mentioned that later more structures were solved for the *Aplysia californica* AChBP complexes, but *L. stagnalis* AChBP has a much higher affinity for α-neurotoxins than AChBPs from other species). First of all, X-ray analysis revealed 5 α-cobratoxin molecules attached at the interfaces between 5 identical subunits (or protomers) of AChBP (Figure 3, A). The major role in the organization of the binding site is played by aromatic residues (so-called "aromatic box") of AChBP. Long before crystallographic studies, protein chemistry and mutagenesis revealed that these aromatic residues were important for binding different agonists and antagonists to diverse muscle-type and neuronal nAChRs. It was proposed that the binding sites are formed by three fragments (A, B, C) of polypeptide chain of one subunit and by three fragments (D, E, F) of the polypeptide chain of the other one on which these aromatic residues are located (see review [100]). The first three fragments in real receptors are on the α-subunits and form the main (principal) binding surface, while the last three are on non-α-subunits and compose the complementary binding surface. In the case of homopentameric receptors like α7 ones, the A-C loops are on the "front surface" of one α7-subunit and D-F on the "back surface" of the neighboring identical subunit. In general, the X-ray structure of the AChBP complex with αcobratoxin is in accord with the earlier ideas on the α-neurotoxin binding to nAChRs formu‐ lated on the basis of chemical modification of α-neurotoxins, their mutagenesis, photoaffinity labeling and mutagenesis of receptors (see reviews [101,102]). Indeed, there is a multipoint binding of α-cobratoxin and the major role, as earlier shown by "wet biochemistry" methods,

The comparison with the NMR and X-ray structures for α-neurotoxins revealed that αcobratoxin did not need to change its conformation dramatically to be accommodated in the binding region of AChBP. On the contrary, the AChBP loop C containing the disulfide between the neighboring cysteines (which is also a characteristic feature of all nAChR α-subunits) had to move to periphery up to 10 Å from the position which it occupied in the AChBP containing no bound ligand. (This movement should be supplemented with essential changes in confor‐ mation of loop F from complementary AChBP protomer.) Moreover, the earlier solved structure of AChBP with such agonist as nicotine revealed that, when agonist comes to the binding site, loop C embraces it and moves closer to the central axis of the molecule [94]. At present there are many crystal structures of various AChBPs in complexes with versatile

structures of complexes with protein and peptide neurotoxins.

is played by the toxin central loop II.

"Mutagenesis" studies of α-conotoxins (in fact not the mutagenesis as such, but substitutions of amino acid residues by solid-phase peptide synthesis) gave information about those residues which are the basis of the high affinity and selectivity to a particular receptor or receptor subgroup. For example, the crucial role of Arg9 in α-conotoxin GI, as well as of Pro6 and Tyr12 in α-conotoxin MI for discriminating the α1/γ- and α1/δ-sites was revealed [80-82]. Interestingly, Arg9 proved important for a neuronal 4/3 α-conotoxin RgIA for its α9α10 nAChR specificity [83]. Similar "mutagenesis" studies resulting in revelation of residues crucial for activity were done also for many other α-conotoxins (ImI, PnIA, MII, GID, Vc1.1, AuIB) [84-89].

Like in the analysis of interactions between different nAChR types and snake venom neuro‐ toxins, when much efforts has been spent by many laboratories to establish the topography of their binding, similar studies have been undertaken to elucidate the mechanism of nAChR recognition by α-conotoxins. Among them were above-mentioned multiple substitutions in the amino acid sequences of naturally occurring α-conotoxins, making their structures more rigid, syntheses of radioactive, fluorescent and photoactivatable derivatives. Combination with mutagenesis of the receptor subunits (pair-wise mutagenesis) gave information about possible contact points between α-neurotoxins and nAChRs, as well as between α-conotoxins and nAChRs. The relevant information can be found in numerous reviews (see, for example, [90-92]), but will not be considered in detail here, because this chapter contains a special section where crystal structures of α-neurotoxins and α-conotoxins in complexes with the relevant biological targets will be discussed.

## **5. Three-dimensional structures of peptide and protein neurotoxins in complexes with the nicotinic receptor models and fragments**

It was already mentioned that the crystal structure of the acetylcholine-binding protein (AChBP) provided an impressing jump in the structural analysis of not only nicotinic acetyl‐ choline receptors but of all other members of the Cys-loop receptor family. This water-soluble protein was found to modulated synaptic transmission in glia of *Lymnaea stagnalis* fresh-water mollusk and was purified using affinity chromatography on a column with the attached αbungarotoxin [93]. Sufficient amounts of AChBP were obtained by heterologous expression and the crystal structure was determined at 2.7 Å resolution [10]. This structure clearly showed that AChBP is an excellent structural model of N-terminal ligand-binding domains of all nAChRs: crystal AChBP was in a pentameric state, similarly to the whole-size nAChRs. In spite of low homology with the amino-acid sequences of extracellular domains of nAChR subunits (not more than 25%), AChBP contains all those amino acid residues which earlier in receptor studies were found essential for interacting with the cholinergic agonists and antagonist. The AChBP crystal structure revealed that such residues are all clustered in the middle of AChBP, at the interfaces between its subunits (or protomers). At present, the X-ray structures of several molluscan AChBPs are known (from *Lymnaea stagnalis*, *Aplysia californica*, *Bulinus truncatus*), as well of their complexes with a wide variety of agonists and antagonists which gave quite a detailed picture of the respective binding sites in these AChBPs and of their contacts with ligands. Biochemical data and computer modeling show convincingly that these structures shed light on the receptor binding sites *per se* and on the ligand disposition in the binding sites of muscle and neuronal nAChRs. The relevant information can be found in recent original papers [94-97] and reviews [98,99], and below we will consider in detail only the crystal structures of complexes with protein and peptide neurotoxins.

Most of muscle 3/5 α-conotoxins can discriminate species-specifically two binding sites on muscle or *Torpedo* nAChRs. For example, α-conotoxins MI, GI or SIA have up to10000 times higher affinity for α1/δ- over α1/γ site in muscle nAChR [76,77]; in contrast to more effective binding of these peptides, although not with such a great difference, to α1/γ site in *Torpedo*

"Mutagenesis" studies of α-conotoxins (in fact not the mutagenesis as such, but substitutions of amino acid residues by solid-phase peptide synthesis) gave information about those residues which are the basis of the high affinity and selectivity to a particular receptor or receptor subgroup. For example, the crucial role of Arg9 in α-conotoxin GI, as well as of Pro6 and Tyr12 in α-conotoxin MI for discriminating the α1/γ- and α1/δ-sites was revealed [80-82]. Interestingly, Arg9 proved important for a neuronal 4/3 α-conotoxin RgIA for its α9α10 nAChR specificity [83]. Similar "mutagenesis" studies resulting in revelation of residues crucial for activity were done also for many other α-conotoxins (ImI, PnIA, MII, GID, Vc1.1, AuIB) [84-89]. Like in the analysis of interactions between different nAChR types and snake venom neuro‐ toxins, when much efforts has been spent by many laboratories to establish the topography of their binding, similar studies have been undertaken to elucidate the mechanism of nAChR recognition by α-conotoxins. Among them were above-mentioned multiple substitutions in the amino acid sequences of naturally occurring α-conotoxins, making their structures more rigid, syntheses of radioactive, fluorescent and photoactivatable derivatives. Combination with mutagenesis of the receptor subunits (pair-wise mutagenesis) gave information about possible contact points between α-neurotoxins and nAChRs, as well as between α-conotoxins and nAChRs. The relevant information can be found in numerous reviews (see, for example, [90-92]), but will not be considered in detail here, because this chapter contains a special section where crystal structures of α-neurotoxins and α-conotoxins in complexes with the relevant

**5. Three-dimensional structures of peptide and protein neurotoxins in**

It was already mentioned that the crystal structure of the acetylcholine-binding protein (AChBP) provided an impressing jump in the structural analysis of not only nicotinic acetyl‐ choline receptors but of all other members of the Cys-loop receptor family. This water-soluble protein was found to modulated synaptic transmission in glia of *Lymnaea stagnalis* fresh-water mollusk and was purified using affinity chromatography on a column with the attached αbungarotoxin [93]. Sufficient amounts of AChBP were obtained by heterologous expression and the crystal structure was determined at 2.7 Å resolution [10]. This structure clearly showed that AChBP is an excellent structural model of N-terminal ligand-binding domains of all nAChRs: crystal AChBP was in a pentameric state, similarly to the whole-size nAChRs. In spite of low homology with the amino-acid sequences of extracellular domains of nAChR subunits (not more than 25%), AChBP contains all those amino acid residues which earlier in receptor studies were found essential for interacting with the cholinergic agonists and antagonist. The

**complexes with the nicotinic receptor models and fragments**

receptor [78,79].

124 Neurochemistry

biological targets will be discussed.

Interestingly, the first AChBP crystal structure in complex with a competitive antagonist was that of *Lymanaea stagnalis* AChBP with bound α-cobratoxin [24]. (In parentheses it may be mentioned that later more structures were solved for the *Aplysia californica* AChBP complexes, but *L. stagnalis* AChBP has a much higher affinity for α-neurotoxins than AChBPs from other species). First of all, X-ray analysis revealed 5 α-cobratoxin molecules attached at the interfaces between 5 identical subunits (or protomers) of AChBP (Figure 3, A). The major role in the organization of the binding site is played by aromatic residues (so-called "aromatic box") of AChBP. Long before crystallographic studies, protein chemistry and mutagenesis revealed that these aromatic residues were important for binding different agonists and antagonists to diverse muscle-type and neuronal nAChRs. It was proposed that the binding sites are formed by three fragments (A, B, C) of polypeptide chain of one subunit and by three fragments (D, E, F) of the polypeptide chain of the other one on which these aromatic residues are located (see review [100]). The first three fragments in real receptors are on the α-subunits and form the main (principal) binding surface, while the last three are on non-α-subunits and compose the complementary binding surface. In the case of homopentameric receptors like α7 ones, the A-C loops are on the "front surface" of one α7-subunit and D-F on the "back surface" of the neighboring identical subunit. In general, the X-ray structure of the AChBP complex with αcobratoxin is in accord with the earlier ideas on the α-neurotoxin binding to nAChRs formu‐ lated on the basis of chemical modification of α-neurotoxins, their mutagenesis, photoaffinity labeling and mutagenesis of receptors (see reviews [101,102]). Indeed, there is a multipoint binding of α-cobratoxin and the major role, as earlier shown by "wet biochemistry" methods, is played by the toxin central loop II.

The comparison with the NMR and X-ray structures for α-neurotoxins revealed that αcobratoxin did not need to change its conformation dramatically to be accommodated in the binding region of AChBP. On the contrary, the AChBP loop C containing the disulfide between the neighboring cysteines (which is also a characteristic feature of all nAChR α-subunits) had to move to periphery up to 10 Å from the position which it occupied in the AChBP containing no bound ligand. (This movement should be supplemented with essential changes in confor‐ mation of loop F from complementary AChBP protomer.) Moreover, the earlier solved structure of AChBP with such agonist as nicotine revealed that, when agonist comes to the binding site, loop C embraces it and moves closer to the central axis of the molecule [94]. At present there are many crystal structures of various AChBPs in complexes with versatile specific or nonselective agonists and antagonists of the muscle-type and neuronal nAChRs and it appears to be a general rule: antagonists versus agonists induce movements of the loop C in the opposite directions.

#### **5.1. X-ray structure of the extracellular domain of muscle nAChR α1 subunit in complex with α-bungarotoxin**

Until now we were considering the X-ray and Electron microscopy structures of closely related but independent objects of studies: acetylcholine binding proteins and *Torpedo* nAChR. It should be emphasized that the structures of bound cholinergic agonists and antagonists until recently were available only for their complexes with AChBPs. That is why when researchers wished to analyze in three-dimensions the interactions of agonists or antagonists with the muscle-type or neuronal nAChRs, they had to rely on computer modeling. Fortunately, one of the bridges between the AChBPs and nAChRs spatial structures has been recently open: the X-ray structure has been determined for the α-bungarotoxin complex with heterologously expressed ligand-binding domain of mouse muscle nAChR α1 subunit [25]. Many laboratories have earlier tried, with the aid of heterologous expression, to obtain ligand-binding domains of α1 or α7 subunits as individual proteins and to determine their three-dimensional structure. Although in certain cases those proteins could bind α-bungarotoxin with relatively high affinity (but not with the nanomolar constants as intact receptors) [103-106], in no case the proteins could be crystallized. In view of the above-said, the work [25] is clearly a break‐ through. Using random mutagenesis, the authors have chosen a protein with a low tendency to aggregation. In spite of its having the mutation of Trp149 (localized in loop B and known to be important for binding agonists and antagonists), the protein could bind α-bungarotoxin. It was namely the complex of α-bungarotoxin rather than the free domain which was success‐ fully crystallized. (Thus, in addition to helping isolate the *Torpedo* nAChR and *L.stagnalis* AChBP, α-neurotoxins played again an important role, this time in crystallization of the nAChR subunit ligand-binding domain.) The structure of the complex has been solved at a very high resolution (1.94 Å) (see Figure 3, B).

70% of the amino-acid residues in *L. stagnalis*AChBP (not touching the less hydrophobic Cys-loop) for residues of the α7 subunit and crystallized this protein in free form and in complex with epibatidine, an potent but nonselective nAChR agonist[108]. The observed pentaoligomeric structure can be considered as the closest proximation to the 3D struc‐ ture of the ligand-binding domain of the true α7 nAChR. Practically the same α7/AChBP chimera has been used to crystallize a complex with α-bungarotoxin [26]. Again, it was a pentaoligomer with 5 attached α-bungarotoxin molecules (see Figure 3, C). In general, disposition of α-bungarotoxin is very close to what was observed for α-bungarotoxin in complex with the α1 domain or for α-cobratoxin complex with the *L. stagnalis* AChBP (compare with Figure 3, B and A). Basing on the high-resolution structure of the α7/AChBPα-bungarotoxin chimera, the authors designed a series of α7 nAChR mutants and from the analysis of their activities and efficiency of α-bungarotoxin binding collected a very detailed information about the intermolecular interactions which ensure the high affinity for αbungarotoxin binding [26]. In particular, they not only confirmed the role of the "aromat‐ ic box", but also revealed the importance of amino-acid residues which in the amino acid

**Figure 3.** Crystal structures of the AChBP/nAChR domain-toxin complexes. Top and side views are in upper and lower lines, respectively. A - α-Cobratoxin bound to *L. stagnalis* AChBP (PDB ID: 1YI5); toxins and proteins are shown in blue and green. B – α-Bungarotoxin bound to the N-terminal domain of nAChR α1 subunit (2QC1); toxin and subunit are shown in blue and red; the sugar moiety presented in this complex were excluded for clarity. C - α-Bungarotoxin bound to the chimeric protein composed of N-terminal domain of nAChR α7 subunit and *L. stagnalis* AChBP (4HQP);

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sequence are direct neighbors of those aromatic residues.

toxins and chimeras are shown in blue and magenta.

**5.3. X-ray structure structures of AChBP complexes with α-conotoxins**

The first X-ray structure of the AChBP complex with α-conotoxin [109] has been solved soon after elucidation of the X-ray structure of the *L. stagnalis* AChBP complex with α-cobratoxin.

Although this domain is a monomer, its spatial structure is very similar to an AChBP protomer in a pentameric complex. A molecule of bound α-bungarotoxin occupies the position similar to that of α-cobratoxin in complex with *L. stagnalis* AChBP (compare Figure 3, A and B). It should be emphasized that in the complex with α1 domain, α-bungarotoxin utilized for interaction only the principal side, while α-cobratoxin in complex with pentameric AChBP has contacts with both principal and complementary sides at the subunit interface. However, instead of this, α-bungarotoxin forms contacts with the sugar moiety present in the nAChR domain but absent in AChBPs.

#### **5.2. X-ray structure of α-bungarotoxin with a chimera of L. stagnalis AChBP/ligand-binding domain of the human α7 subunit**

This work can be considered as a further development of the recent breakthrough in the analysis of ligand binding domains of nAChRs when an important step was done in ascending from models to true receptors. The authors of [107] managed to substitute about

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specific or nonselective agonists and antagonists of the muscle-type and neuronal nAChRs and it appears to be a general rule: antagonists versus agonists induce movements of the loop

**5.1. X-ray structure of the extracellular domain of muscle nAChR α1 subunit in complex**

Until now we were considering the X-ray and Electron microscopy structures of closely related but independent objects of studies: acetylcholine binding proteins and *Torpedo* nAChR. It should be emphasized that the structures of bound cholinergic agonists and antagonists until recently were available only for their complexes with AChBPs. That is why when researchers wished to analyze in three-dimensions the interactions of agonists or antagonists with the muscle-type or neuronal nAChRs, they had to rely on computer modeling. Fortunately, one of the bridges between the AChBPs and nAChRs spatial structures has been recently open: the X-ray structure has been determined for the α-bungarotoxin complex with heterologously expressed ligand-binding domain of mouse muscle nAChR α1 subunit [25]. Many laboratories have earlier tried, with the aid of heterologous expression, to obtain ligand-binding domains of α1 or α7 subunits as individual proteins and to determine their three-dimensional structure. Although in certain cases those proteins could bind α-bungarotoxin with relatively high affinity (but not with the nanomolar constants as intact receptors) [103-106], in no case the proteins could be crystallized. In view of the above-said, the work [25] is clearly a break‐ through. Using random mutagenesis, the authors have chosen a protein with a low tendency to aggregation. In spite of its having the mutation of Trp149 (localized in loop B and known to be important for binding agonists and antagonists), the protein could bind α-bungarotoxin. It was namely the complex of α-bungarotoxin rather than the free domain which was success‐ fully crystallized. (Thus, in addition to helping isolate the *Torpedo* nAChR and *L.stagnalis* AChBP, α-neurotoxins played again an important role, this time in crystallization of the nAChR subunit ligand-binding domain.) The structure of the complex has been solved at a

Although this domain is a monomer, its spatial structure is very similar to an AChBP protomer in a pentameric complex. A molecule of bound α-bungarotoxin occupies the position similar to that of α-cobratoxin in complex with *L. stagnalis* AChBP (compare Figure 3, A and B). It should be emphasized that in the complex with α1 domain, α-bungarotoxin utilized for interaction only the principal side, while α-cobratoxin in complex with pentameric AChBP has contacts with both principal and complementary sides at the subunit interface. However, instead of this, α-bungarotoxin forms contacts with the sugar moiety present in the nAChR

**5.2. X-ray structure of α-bungarotoxin with a chimera of L. stagnalis AChBP/ligand-binding**

This work can be considered as a further development of the recent breakthrough in the analysis of ligand binding domains of nAChRs when an important step was done in ascending from models to true receptors. The authors of [107] managed to substitute about

C in the opposite directions.

very high resolution (1.94 Å) (see Figure 3, B).

domain but absent in AChBPs.

**domain of the human α7 subunit**

**with α-bungarotoxin**

126 Neurochemistry

**Figure 3.** Crystal structures of the AChBP/nAChR domain-toxin complexes. Top and side views are in upper and lower lines, respectively. A - α-Cobratoxin bound to *L. stagnalis* AChBP (PDB ID: 1YI5); toxins and proteins are shown in blue and green. B – α-Bungarotoxin bound to the N-terminal domain of nAChR α1 subunit (2QC1); toxin and subunit are shown in blue and red; the sugar moiety presented in this complex were excluded for clarity. C - α-Bungarotoxin bound to the chimeric protein composed of N-terminal domain of nAChR α7 subunit and *L. stagnalis* AChBP (4HQP); toxins and chimeras are shown in blue and magenta.

70% of the amino-acid residues in *L. stagnalis*AChBP (not touching the less hydrophobic Cys-loop) for residues of the α7 subunit and crystallized this protein in free form and in complex with epibatidine, an potent but nonselective nAChR agonist[108]. The observed pentaoligomeric structure can be considered as the closest proximation to the 3D struc‐ ture of the ligand-binding domain of the true α7 nAChR. Practically the same α7/AChBP chimera has been used to crystallize a complex with α-bungarotoxin [26]. Again, it was a pentaoligomer with 5 attached α-bungarotoxin molecules (see Figure 3, C). In general, disposition of α-bungarotoxin is very close to what was observed for α-bungarotoxin in complex with the α1 domain or for α-cobratoxin complex with the *L. stagnalis* AChBP (compare with Figure 3, B and A). Basing on the high-resolution structure of the α7/AChBPα-bungarotoxin chimera, the authors designed a series of α7 nAChR mutants and from the analysis of their activities and efficiency of α-bungarotoxin binding collected a very detailed information about the intermolecular interactions which ensure the high affinity for αbungarotoxin binding [26]. In particular, they not only confirmed the role of the "aromat‐ ic box", but also revealed the importance of amino-acid residues which in the amino acid sequence are direct neighbors of those aromatic residues.

#### **5.3. X-ray structure structures of AChBP complexes with α-conotoxins**

The first X-ray structure of the AChBP complex with α-conotoxin [109] has been solved soon after elucidation of the X-ray structure of the *L. stagnalis* AChBP complex with α-cobratoxin.

had different orientations [96]. Such multiplicity was first thought to be inherent only in partial agonists, but later altering dispositions in the 5 AChBP binding sites were ob‐ served for the complexes of such alkaloid antagonists as strychnine and d-tubocurarine [113]. Moreover, in several binding sites two alkaloid molecules managed to be accommo‐

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Variations of the ligand orientation in the binding sites of AChBPs and nAChRs are of undoubted interest. In the *A. californica* AChBP complex, all 5 bound α-conotoxin PnIA[A10L, D14K] molecules had the same conformation and orientation. This was also true for the later solved structures of α-conotoxin ImI complexes [114,115] (see Figure 4, B). These structures (very similar to those of α-conotoxin PnIA[A10L, D14K]) confirmed that, although bound αconotoxin PnIA analog had two substitutions and was in this respect "unnatural α-conotoxin", the X-ray structure of its complex correctly revealed the structural principles of the α-cono‐ toxin-AChBP recognition. Fine adjustments of such a recognition were brought to light by the structure of *A. californica* AChBP complex with the α-conotoxin TxIA[A10L] [116]. In general, the structure of this complex was very similar to those of α-conotoxin PnIA[A10L, D14K] or α-conotoxin ImI, but with a noticeable difference: this α-conotoxin derivative occupied exactly the same region as the two above-mentioned α-conotoxins, but it was turned around the central axis by about 20 degrees. The authors proposed that such rotation reflects certain differences in the selectivity of this particular α-conotoxin [116]. The latest published structure of the AChBP complex with α-conotoxin (November 2013) is announced by the Protein Data Bank (PDB) the structure with ID - 4EZ1. This is a complex of *A. californica* AChBP with α-conotoxin BuIA [117]. Despite the fact that α-conotoxin BuIA is a member of other subgroup of αconotoxins (4/4) its position and orientation in the complex with AChBP (Figure 4, C) very close to that of both α-conotoxin PnIA analog (4/7 subgroup) and α-conotoxin ImI (4/3 subgroup) (compare Figure 4, A, B and C). In any case, from the four solved X-ray structures for AChBP complexes with α-conotoxins it followed that some variations in their attachment are possible. It might be expected that variations may be even more pronounced when αconotoxins interact with true nAChRs, especially with heteroligomeric ones having different

Indeed, interpretation of the cross-linking of photoactivatable derivative of α-conotoxin GI to *Torpedo californica* nAChR in terms of the model built on the basis of the X-ray structure of the AChBP complex with α-conotoxin PnIA[A10L, D14K], suggested that for bound α-conotoxin two orientations are possible where the disposition of photoactivatable group differs by about 90 degrees [118]. Later a similar situation was demonstrated for an agonist, namely for the photoactivatable derivative of epibatidine [119]. This compound was shown to bind to only one site in the *T. californica* nAChR, but to 2 sites in the neuronal α4β2 nAChR which presumes two different dispositions of the bound ligand [119]. Naturally, cross-linking is not such a direct evidence as the X-ray structure, but the latter are available only for the AChBP complexes and the multiplicity of alkaloid antagonist orientations in the frames of one AChBP molecule [113]

dated simultaneously [113].

subunit interfaces.

has been already mentioned.

**Figure 4.** Spatial organization of complexes of α-conotoxins from different groups and *A. californica* AChBP derived from their crystal structures. Only two adjacent monomers of AChBP colored in cyan and yellow for clarity in side views are presented. All α-conotoxins are shown in magenta. A – complex with α-conotoxin PnIA[A10L, D14K] variant from 4/7 α-conotoxin group (PDB ID: 2BR8). B – complex with α-conotoxin ImI from 4/3 α-conotoxin group (2C9T). C - com‐ plex with α-conotoxin BuIA from 4/4 α-conotoxin group (4EZ1).

In this case it was another AChBP, namely the protein from the marine mollusk *Aplysia californica* [110]. First of all, contrary to the α-cobratoxin complex, it was a high-resolution (2.4 Å) structure and, secondly, it was the first X-ray structure for a representative of the huge conotoxin library in complex with a biological target. The crystals were raised for the complex of α-conotoxin PnIA analog having two substitutions ([A10L] and [D14K]) which had high affinity both for *L. stagnalis* and *A. californica* AChBPs and potently inhibited acetylcholineinduced currents in α7 nAChRs expressed in oocytes [109].

Hydrophobic contacts were found to play the major role in the interaction of α-conotoxin PnIA[A10L, D14K] with *A. californica* AChBP (Figure 4, A). As in other AChBP complexes with agonists or antagonists, at the principal side the contacts are formed mainly by highly conserved aromatic amino acid residues - Trp145, Tyr186, Tyr193. At the complementary side the contributions are from aliphatic residues (Val106, Met114, Ile116). It should be stressed again that loop C in the complex with α-conotoxin moves to the periphery of the AChBP molecule by more than 10 Å, as compared with its disposition in the "apo" form of *A. californica* AChBP. A similar shift was also observed, as mentioned above, for the αcobratoxin complex [24], as well as for the majority of AChBP complexes with other antagonists (see reviews [98,111,112]). Thus, the conclusion that the most obvious distinc‐ tion between the first steps in the binding modes of agonists versus antagonists is the induced movement of the loop C (to the central axis for the former and outwards for the latter) appears to be correct. However, there are some deviations from this trend: for example, strychnine is an antagonist both of the nAChRs and glycine receptors, but in the case of its complex with the *A. californica* AChBP, the loop C shift to the periphery was only very slight [113]. The changes in the disposition of the loop C were not pronounced also for AChBP complexes with partial agonists [96].

Another interesting feature of AChBP complexes was for the first time observed with partial agonists: in distinct binding sites within a pentameric AChBP molecule these compounds had different orientations [96]. Such multiplicity was first thought to be inherent only in partial agonists, but later altering dispositions in the 5 AChBP binding sites were ob‐ served for the complexes of such alkaloid antagonists as strychnine and d-tubocurarine [113]. Moreover, in several binding sites two alkaloid molecules managed to be accommo‐ dated simultaneously [113].

Variations of the ligand orientation in the binding sites of AChBPs and nAChRs are of undoubted interest. In the *A. californica* AChBP complex, all 5 bound α-conotoxin PnIA[A10L, D14K] molecules had the same conformation and orientation. This was also true for the later solved structures of α-conotoxin ImI complexes [114,115] (see Figure 4, B). These structures (very similar to those of α-conotoxin PnIA[A10L, D14K]) confirmed that, although bound αconotoxin PnIA analog had two substitutions and was in this respect "unnatural α-conotoxin", the X-ray structure of its complex correctly revealed the structural principles of the α-cono‐ toxin-AChBP recognition. Fine adjustments of such a recognition were brought to light by the structure of *A. californica* AChBP complex with the α-conotoxin TxIA[A10L] [116]. In general, the structure of this complex was very similar to those of α-conotoxin PnIA[A10L, D14K] or α-conotoxin ImI, but with a noticeable difference: this α-conotoxin derivative occupied exactly the same region as the two above-mentioned α-conotoxins, but it was turned around the central axis by about 20 degrees. The authors proposed that such rotation reflects certain differences in the selectivity of this particular α-conotoxin [116]. The latest published structure of the AChBP complex with α-conotoxin (November 2013) is announced by the Protein Data Bank (PDB) the structure with ID - 4EZ1. This is a complex of *A. californica* AChBP with α-conotoxin BuIA [117]. Despite the fact that α-conotoxin BuIA is a member of other subgroup of αconotoxins (4/4) its position and orientation in the complex with AChBP (Figure 4, C) very close to that of both α-conotoxin PnIA analog (4/7 subgroup) and α-conotoxin ImI (4/3 subgroup) (compare Figure 4, A, B and C). In any case, from the four solved X-ray structures for AChBP complexes with α-conotoxins it followed that some variations in their attachment are possible. It might be expected that variations may be even more pronounced when αconotoxins interact with true nAChRs, especially with heteroligomeric ones having different subunit interfaces.

In this case it was another AChBP, namely the protein from the marine mollusk *Aplysia californica* [110]. First of all, contrary to the α-cobratoxin complex, it was a high-resolution (2.4 Å) structure and, secondly, it was the first X-ray structure for a representative of the huge conotoxin library in complex with a biological target. The crystals were raised for the complex of α-conotoxin PnIA analog having two substitutions ([A10L] and [D14K]) which had high affinity both for *L. stagnalis* and *A. californica* AChBPs and potently inhibited acetylcholine-

**Figure 4.** Spatial organization of complexes of α-conotoxins from different groups and *A. californica* AChBP derived from their crystal structures. Only two adjacent monomers of AChBP colored in cyan and yellow for clarity in side views are presented. All α-conotoxins are shown in magenta. A – complex with α-conotoxin PnIA[A10L, D14K] variant from 4/7 α-conotoxin group (PDB ID: 2BR8). B – complex with α-conotoxin ImI from 4/3 α-conotoxin group (2C9T). C - com‐

Hydrophobic contacts were found to play the major role in the interaction of α-conotoxin PnIA[A10L, D14K] with *A. californica* AChBP (Figure 4, A). As in other AChBP complexes with agonists or antagonists, at the principal side the contacts are formed mainly by highly conserved aromatic amino acid residues - Trp145, Tyr186, Tyr193. At the complementary side the contributions are from aliphatic residues (Val106, Met114, Ile116). It should be stressed again that loop C in the complex with α-conotoxin moves to the periphery of the AChBP molecule by more than 10 Å, as compared with its disposition in the "apo" form of *A. californica* AChBP. A similar shift was also observed, as mentioned above, for the αcobratoxin complex [24], as well as for the majority of AChBP complexes with other antagonists (see reviews [98,111,112]). Thus, the conclusion that the most obvious distinc‐ tion between the first steps in the binding modes of agonists versus antagonists is the induced movement of the loop C (to the central axis for the former and outwards for the latter) appears to be correct. However, there are some deviations from this trend: for example, strychnine is an antagonist both of the nAChRs and glycine receptors, but in the case of its complex with the *A. californica* AChBP, the loop C shift to the periphery was only very slight [113]. The changes in the disposition of the loop C were not pronounced

Another interesting feature of AChBP complexes was for the first time observed with partial agonists: in distinct binding sites within a pentameric AChBP molecule these compounds

induced currents in α7 nAChRs expressed in oocytes [109].

plex with α-conotoxin BuIA from 4/4 α-conotoxin group (4EZ1).

128 Neurochemistry

also for AChBP complexes with partial agonists [96].

Indeed, interpretation of the cross-linking of photoactivatable derivative of α-conotoxin GI to *Torpedo californica* nAChR in terms of the model built on the basis of the X-ray structure of the AChBP complex with α-conotoxin PnIA[A10L, D14K], suggested that for bound α-conotoxin two orientations are possible where the disposition of photoactivatable group differs by about 90 degrees [118]. Later a similar situation was demonstrated for an agonist, namely for the photoactivatable derivative of epibatidine [119]. This compound was shown to bind to only one site in the *T. californica* nAChR, but to 2 sites in the neuronal α4β2 nAChR which presumes two different dispositions of the bound ligand [119]. Naturally, cross-linking is not such a direct evidence as the X-ray structure, but the latter are available only for the AChBP complexes and the multiplicity of alkaloid antagonist orientations in the frames of one AChBP molecule [113] has been already mentioned.

#### **6. Summary**

In this chapter we tried to briefly present almost a 50-year history of using protein and peptide neurotoxins in fundamental and practical studies of nicotinic acetylcholine receptors (nAChRs). It was shown that the discovery of α-neurotoxins in the snake venoms was an extremely important step which made possible identification and isolation in individual form of the first nAChR from the *Torpedo* ray electric organ. Many laboratories comprehensively analyzed this receptor and it soon became clear that it is an appropriate model for nAChRs of all classes, namely muscle, neuronal and the so-called "non-neuronal" ones. Later, in addition to the three-finger α-neurotoxins, new shorter and smaller but not less efficient tools were found: namely, among a huge family of various peptides in the venoms of marine *Conus* mollusks, one particular group happened to be invaluable for research on nAChRs. Here we speak about α-conotoxins which not only discriminate the muscle-type from neuronal nAChRs, but some of them even are selective towards a particular neuronal nAChR subtype. One should not think that the discovery of α-conotoxins put the α-neurotoxins into archives. First of all, even to-day α-bungarotoxin and its radioactive and fluorescent derivatives are the most reliable tools for identification and measuring the levels of the functional α7 nAChRs. Secondly, α-neurotoxins played another leading role a decade ago helping to purify the acetylcholine-binding protein (AChBP). The discovery and the X-ray structure of this protein, an ideal model for the ligand-binding domains of all nAChRs, was the major breakthrough in elucidating the three-dimensional structure of nAChRs and especially of their ligand-binding site topography. Our chapter also presented the data on the crystal structures of AChBP complexes both with α-neurotoxins and α-conotoxins that gave information about the topography of their interactions with the key residues in the binding site, thus providing a basis for new drug design. The next step was the establishment of the crystal structures of αneurotoxins with chimera of AChBP and α7 nAChR ligand-binding domain, which can be considered as a good mimic of the true α7 receptor, as well as the X-ray structure of the αbungarotoxin complex with a mutated nAChR α1 subunit extracellular domain. In this chapter we were not discussing the bacterial pentameric ligand-gated ion channels (belonging to the same family as nAChRs), but at present not only high-resolution X-ray structures are available for them, but also for their complexes with different ligands. In particular, one of such receptors (ELIC) happened to be a close analog of the mammalian GABA-A receptors. We might hope that one day high resolution structures become available for nAChRs or their homologs in complexes with α-neurotoxins and/or α-conotoxins, to which the chapter is devoted. It will give new life to these still invaluable tools in fundamental research on nAChRs and in numerous practical applications.

**Author details**

Moscow, Russian Federation

2012;287(48) 40207-40215.

Nature 2006;440(7083) 448-455.

Pharmacology 1988;34(5) 643-650.

ical Sciences 2008;106(2) 186-192.

10.1101/cshperspect.a012112.

Opinion in Pharmacology 2009;9(3) 306-310.

Academy of Sciences of the USA 1987;84(12) 4318-4321.

and Igor Kasheverov

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences,

Peptide and Protein Neurotoxin Toolbox in Research on Nicotinic Acetylcholine Receptors

http://dx.doi.org/10.5772/58240

131

[1] Changeux JP. The nicotinic acetylcholine receptor: the founding father of the pen‐ tameric ligand-gated ion channel superfamily. Journal of Biological Chemistry

[2] Kalamida D, Poulas K, Avramopoulou V, Fostieri E, Lagoumintzis G, Lazaridis K, Sideri A, Zouridakis M, Tzartos SJ. Muscle and neuronal nicotinic acetylcholine re‐ ceptors. Structure, function and pathogenicity. FEBS Journal 2007;274(15) 3799-3845.

[3] Tsetlin V, Hucho F. Nicotinic acetylcholine receptors at atomic resolution. Current

[4] Sine SM, Engel AG. Recent advances in Cys-loop receptor structure and function.

[5] Gershoni JM. Expression of the alpha-bungarotoxin binding site of the nicotinic ace‐ tylcholine receptor by Escherichia coli transformants. Proceedings of the National

[6] Wilson PT, Hawrot E, Lentz TL. Distribution of alpha-bungarotoxin binding sites over residues 173-204 of the alpha subunit of the acetylcholine receptor. Molecular

[7] Wessler I, Kirkpatrick CJ. Acetylcholine beyond neurons: the non-neuronal choliner‐ gic system in humans. British Journal of Pharmacology 2008;154(8) 1558-1571.

[8] Fujii T, Takada-Takatori Y, Kawashima K. Basic and clinical aspects of non-neuronal acetylcholine: expression of an independent, non-neuronal cholinergic system in lymphocytes and its clinical significance in immunotherapy. Journal of Pharmacolog‐

[9] Picciotto MR, Kenny PJ. Molecular mechanisms underlying behaviors related to nico‐ tine addiction. Cold Spring Harbor Perspectives in Medicine 2013;3(1) a012112. doi:

\*Address all correspondence to: vits@mx.ibch.ru

Victor Tsetlin\*

**References**

#### **Acknowledgements**

The research was supported by RFBR grants and the grants of Presidium of RAS "Molecular and cellular biology" and "Fundamental principles of medicine".

## **Author details**

**6. Summary**

130 Neurochemistry

numerous practical applications.

**Acknowledgements**

In this chapter we tried to briefly present almost a 50-year history of using protein and peptide neurotoxins in fundamental and practical studies of nicotinic acetylcholine receptors (nAChRs). It was shown that the discovery of α-neurotoxins in the snake venoms was an extremely important step which made possible identification and isolation in individual form of the first nAChR from the *Torpedo* ray electric organ. Many laboratories comprehensively analyzed this receptor and it soon became clear that it is an appropriate model for nAChRs of all classes, namely muscle, neuronal and the so-called "non-neuronal" ones. Later, in addition to the three-finger α-neurotoxins, new shorter and smaller but not less efficient tools were found: namely, among a huge family of various peptides in the venoms of marine *Conus* mollusks, one particular group happened to be invaluable for research on nAChRs. Here we speak about α-conotoxins which not only discriminate the muscle-type from neuronal nAChRs, but some of them even are selective towards a particular neuronal nAChR subtype. One should not think that the discovery of α-conotoxins put the α-neurotoxins into archives. First of all, even to-day α-bungarotoxin and its radioactive and fluorescent derivatives are the most reliable tools for identification and measuring the levels of the functional α7 nAChRs. Secondly, α-neurotoxins played another leading role a decade ago helping to purify the acetylcholine-binding protein (AChBP). The discovery and the X-ray structure of this protein, an ideal model for the ligand-binding domains of all nAChRs, was the major breakthrough in elucidating the three-dimensional structure of nAChRs and especially of their ligand-binding site topography. Our chapter also presented the data on the crystal structures of AChBP complexes both with α-neurotoxins and α-conotoxins that gave information about the topography of their interactions with the key residues in the binding site, thus providing a basis for new drug design. The next step was the establishment of the crystal structures of αneurotoxins with chimera of AChBP and α7 nAChR ligand-binding domain, which can be considered as a good mimic of the true α7 receptor, as well as the X-ray structure of the αbungarotoxin complex with a mutated nAChR α1 subunit extracellular domain. In this chapter we were not discussing the bacterial pentameric ligand-gated ion channels (belonging to the same family as nAChRs), but at present not only high-resolution X-ray structures are available for them, but also for their complexes with different ligands. In particular, one of such receptors (ELIC) happened to be a close analog of the mammalian GABA-A receptors. We might hope that one day high resolution structures become available for nAChRs or their homologs in complexes with α-neurotoxins and/or α-conotoxins, to which the chapter is devoted. It will give new life to these still invaluable tools in fundamental research on nAChRs and in

The research was supported by RFBR grants and the grants of Presidium of RAS "Molecular

and cellular biology" and "Fundamental principles of medicine".

Victor Tsetlin\* and Igor Kasheverov

\*Address all correspondence to: vits@mx.ibch.ru

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation

## **References**


[10] Brejc K, van Dijk WJ, Klaassen RV, Schuurmans M, van Der Oost J, Smit AB, Sixma TK. Crystal structure of an ACh-binding protein reveals the ligand-binding domain of nicotinic receptors. Nature 2001;411(6835) 269-276.

[23] Nickitenko AV, Michailov AM, Betzel C, Wilson KS. Three-dimensional structure of neurotoxin-1 from Naja naja oxiana venom at 1.9 Å resolution. FEBS Letters

Peptide and Protein Neurotoxin Toolbox in Research on Nicotinic Acetylcholine Receptors

http://dx.doi.org/10.5772/58240

133

[24] Bourne Y, Talley TT, Hansen SB, Taylor P, Marchot P.Crystal structure of a Cbtx-AChBP complex reveals essential interactions between snake alpha-neurotoxins and

[25] Dellisanti CD, Yao Y, Stroud JC, Wang ZZ, Chen L. Crystal structure of the extracel‐ lular domain of nAChR alpha1 bound to alpha-bungarotoxin at 1.94 Å resolution.

[26] Huang S, Li SX, Bren N, Cheng K, Gomoto R, Chen L, Sine SM. Complex between αbungarotoxin and an α7 nicotinic receptor ligand-binding domain chimaera. Bio‐

[27] Dewan JC, Grant GA, Sacchettini JC. Crystal structure of κ-bungarotoxin at 2.3-A res‐

[28] Chiappinelli VA, Weaver WR, McLane KE, Conti-Fine BM, Fiordalisi JJ, Grant GA. Binding of native κ-neurotoxins and site-directed mutants to nicotinic acetylcholine

[29] Servent D, Winckler-Dietrich V, Hu HY, Kessler P, Drevet P, Bertrand D, Ménez A. Only snake curaremimetic toxins with a fifth disulfide bond have high affinity for the neuronal α7 nicotinic receptor. Journal of Biological Chemistry 1997;272(39)

[30] Grant GA, Luetje CW, Summers R, Xu XL. Differential roles for disulfide bonds in the structural integrity and biological activity of κ-bungarotoxin, a neuronal nicotinic

[31] Mourier G, Servent D, Zinn-Justin S, Ménez A. Chemical engineering of a three-fin‐ gered toxin with anti-α7 neuronal acetylcholine receptor activity. Protein Engineer‐

[32] Lyukmanova EN, Shenkarev ZO, Schulga AA, Ermolyuk YS, Mordvintsev DY, Utkin YN, Shoulepko MA, Hogg RC, Bertrand D, Dolgikh DA, Tsetlin VI, Kirpichnikov MP. Bacterial expression, NMR, and electrophysiology analysis of chimeric short/ long-chain α-neurotoxins acting on neuronal nicotinic receptors. Journal of Biological

[33] Roy A, Zhou X, Chong MZ, D'hoedt D, Foo CS, Rajagopalan N, Nirthanan S, Ber‐ trand D, Sivaraman J, Kini RM. Structural and functional characterization of a novel homodimeric three-finger neurotoxin from the venom of Ophiophagus hannah (king

cobra). Journal of Biological Chemistry 2010;285(11) 8302-8315.

acetylcholine receptor antagonist. Biochemistry 1998;37(35) 12166-12171.

nicotinic receptors. EMBO Journal 2005;24(8) 1512-1522.

Nature Neuroscience 2007;10(8) 953-962.

chemical Journal 2013;454(2) 303-310.

olution. Biochemistry 1994;33(44) 13147-13154.

receptors. Toxicon 1996;34(11-12) 1243-1256.

1993;320(2) 111-117.

24279-24286.

ing 2000;13(3) 217-225.

Chemistry 2007;282(34) 24784-24791.


[23] Nickitenko AV, Michailov AM, Betzel C, Wilson KS. Three-dimensional structure of neurotoxin-1 from Naja naja oxiana venom at 1.9 Å resolution. FEBS Letters 1993;320(2) 111-117.

[10] Brejc K, van Dijk WJ, Klaassen RV, Schuurmans M, van Der Oost J, Smit AB, Sixma TK. Crystal structure of an ACh-binding protein reveals the ligand-binding domain

[11] Hilf RJ, Dutzler R. X-ray structure of a prokaryotic pentameric ligand-gated ion chan‐

[12] Hilf RJ, Dutzler R. Structure of a potentially open state of a proton-activated pen‐

[13] Bocquet N, Nury H, Baaden M, Le Poupon C, Changeux JP, Delarue M, Corringer PJ. X-ray structure of a pentameric ligand-gated ion channel in an apparently open con‐

[14] Hibbs RE, Gouaux E. Principles of activation and permeation in an anion-selective

[15] Chang CC. Looking back on the discovery of α-bungarotoxin. Journal of Biomedical

[16] Tsetlin V. Snake venom α-neurotoxins and other 'three-finger' proteins. European

[17] Kini RM, Doley R. Structure, function and evolution of three-finger toxins: mini pro‐

[18] Low BW, Preston HS, Sato A, Rosen LS, Searl JE, Rudko AD, Richardson JS. Three dimensional structure of erabutoxin b neurotoxic protein: inhibitor of acetylcholine receptor. Proceedings of the National Academy of Sciences of the USA 1976;73(9)

[19] Tsernoglou D, Petsko GA. Three-dimensional structure of neurotoxin a from venom of the Philippines sea snake. Proceedings of the National Academy of Sciences of the

[20] Love R, Stroud R. The crystal structure of α-bungarotoxin at 2.5 Å resolution: relation to solution structure and binding to acetylcholine receptor. Protein Engineering

[21] Moise L, Piserchio A, Basus VJ, Hawrot E. NMR structural analysis of α-bungarotox‐ in and its complex with the principal α-neurotoxin-binding sequence on the α7 subu‐ nit of a neuronal nicotinic acetylcholine receptor. Journal of Biological Chemistry

[22] Betzel C, Lange G, Pal GP, Wilson KS, Maelicke A, Saenger W. The refined crystal structure of alpha-cobratoxin from Naja naja siamensis at 2.4-Å resolution. Journal of

tameric ligand-gated ion channel. Nature 2009;457(7225) 115-118.

of nicotinic receptors. Nature 2001;411(6835) 269-276.

nel. Nature 2008;452(7185) 375-379.

132 Neurochemistry

formation. Nature 2009;457(7225) 111-114.

Sciences 1999;6(6) 368-375.

2991-2994.

USA 1977;74(3) 971-974.

2002;277(14) 12406-12417.

Biological Chemistry 1991;266(32) 21530-21536.

1986;1(1) 37-46.

Cys-loop receptor. Nature 2011;474(7349) 54-60.

Journal of Biochemistry 1999;264(2) 281-286.

teins with multiple targets. Toxicon 2010;56(6) 855-867.


[34] Kukhtina VV, Weise C, Muranova TA, Starkov VG, Franke P, Hucho F, Wnendt S, Gillen C, Tsetlin VI, Utkin YN. Muscarinic toxin-like proteins from cobra venom. Eu‐ ropean Journal of Biochemistry 2000;267(23) 6784-6789.

[44] Ménez A. Functional architectures of animal toxins: a clue to drug design? Toxicon

Peptide and Protein Neurotoxin Toolbox in Research on Nicotinic Acetylcholine Receptors

http://dx.doi.org/10.5772/58240

135

[45] Olianas MC, Adem A, Karlsson E, Onali P. Action of the muscarinic toxin MT7 on agonist-bound muscarinic M1 receptors. European Journal of Pharmacology

[46] Marquer C, Fruchart-Gaillard C, Letellier G, Marcon E, Mourier G, Zinn-Justin S, Mé‐ nez A, Servent D, Gilquin B. Structural model of ligand-G protein-coupled receptor (GPCR) complex based on experimental double mutant cycle data: MT7 snake toxin bound to dimeric hM1 muscarinic receptor. Journal of Biological Chemistry

[47] de Weille JR, Schweitz H, Maes P, Tartar A, Lazdunski M. Calciseptine, a peptide iso‐ lated from black mamba venom, is a specific blocker of the L-type calcium channel. Proceedings of the National Academy of Sciences of the USA 1991;88(6) 2437-2440.

[48] Albrand JP, Blackledge MJ, Pascaud F, Hollecker M, Marion D. NMR and restrained molecular dynamics study of the three-dimensional solution structure of toxin FS2, a specific blocker of the L-type calcium channel, isolated from black mamba venom. Bi‐

[49] Bourne Y, Taylor P, Marchot P. Acetylcholinesterase inhibition by fasciculin: crystal

[50] Harel M, Kleywegt GJ, Ravelli RB, Silman I, Sussman JL. Crystal structure of an ace‐ tylcholinesterase-fasciculin complex: interaction of a three-fingered toxin from snake

[51] Kumar TK, Jayaraman G, Lee CS, Arunkumar AI, Sivaraman T, Samuel D, Yu C. Snake venom cardiotoxins-structure, dynamics, function and folding. Journal of Bio‐

[52] Konshina AG, Dubovskii PV, Efremov RG. Structure and dynamics of cardiotoxins.

[53] Osipov AV, Astapova MV, Tsetlin VI, Utkin YN. The first representative of glycosy‐ lated three-fingered toxins. Cytotoxin from the Naja kaouthia cobra venom. Europe‐

[54] Quinton L, Girard E, Maiga A, Rekik M, Lluel P, Masuyer G, Larregola M, Marquer C, Ciolek J, Magnin T, Wagner R, Molgó J, Thai R, Fruchart-Gaillard C, Mourier G, Chamot-Rooke J, Ménez A, Palea S, Servent D, Gilles N. Isolation and pharmacologi‐ cal characterization of AdTx1, a natural peptide displaying specific insurmountable antagonism of the alpha1A-adrenoceptor. British Journal of Pharmacology

[55] Maïga A, Mourier G, Quinton L, Rouget C, Gales C, Denis C, Lluel P, Sénard JM, Pa‐ lea S, Servent D, Gilles N. G protein-coupled receptors, an unexploited animal toxin

1998;36(11) 1557-1572.

2004;487(1-3) 65-72.

2011;286(36) 31661-31675.

ochemistry 1995;34(17) 5923-5937.

structure of the complex. Cell 1995;83(3) 503-512.

venom with its target. Structure 1995;3(12) 1355-1366.

molecular Structure and Dynamics 1997;15(3) 431-463.

Current Protein and Peptide Science 2012;13(6) 570-584.

an Journal of Biochemistry 2004;271(10) 2018-2027.

2010;159(2) 316-325.


[44] Ménez A. Functional architectures of animal toxins: a clue to drug design? Toxicon 1998;36(11) 1557-1572.

[34] Kukhtina VV, Weise C, Muranova TA, Starkov VG, Franke P, Hucho F, Wnendt S, Gillen C, Tsetlin VI, Utkin YN. Muscarinic toxin-like proteins from cobra venom. Eu‐

[35] Osipov AV, Kasheverov IE, Makarova YV, Starkov VG, Vorontsova OV, Ziganshin RK, Andreeva TV, Serebryakova MV, Benoit A, Hogg RC, Bertrand D, Tsetlin VI, Ut‐ kin YN. Naturally occurring disulfide-bound dimers of three-fingered toxins: a para‐ digm for biological activity diversification. Journal of Biological Chemistry

[36] Osipov AV, Rucktooa P, Kasheverov IE, Filkin SY, Starkov VG, Andreeva TV, Sixma TK, Bertrand D, Utkin YN, Tsetlin VI. Dimeric α-cobratoxin X-ray structure: localiza‐ tion of intermolecular disulfides and possible mode of binding to nicotinic acetylcho‐

[37] Pawlak J, Mackessy SP, Sixberry NM, Stura EA, Le Du MH, Ménez R, Foo CS, Ménez A, Nirthanan S, Kini RM. Irditoxin, a novel covalently linked heterodimeric three-fin‐ ger toxin with high taxon-specific neurotoxicity. FASEB Journal 2009;23(2) 534-545.

[38] Nirthanan S, Gopalakrishnakone P, Gwee MC, Khoo HE, Kini RM. Non-conventional toxins from Elapid venoms. British Journal of Pharmacology 2003;139(4) 832-844.

[39] Utkin YN, Kukhtina VV, Kryukova EV, Chiodini F, Bertrand D, Methfessel C, Tsetlin VI. "Weak toxin" from Naja kaouthia is a nontoxic antagonist of α7 and muscle-type nicotinic acetylcholine receptors. Journal of Biological Chemistry 2001;276(19)

[40] Nirthanan S, Charpantier E, Gopalakrishnakone P, Gwee MC, Khoo HE, Cheah LS, Bertrand D, Kini RM. Candoxin, a novel toxin from Bungarus candidus, is a reversi‐ ble antagonist of muscle (αβγδ) but a poorly reversible antagonist of neuronal α7 nicotinic acetylcholine receptors. Journal of Biological Chemistry 2002;277(20)

[41] Pawlak J, Mackessy SP, Fry BG, Bhatia M, Mourier G, Fruchart-Gaillard C, Servent D, Ménez R, Stura E, Ménez A, Kini RM. Denmotoxin, a three-finger toxin from the co‐ lubrid snake Boiga dendrophila (Mangrove Catsnake) with bird-specific activity.

[42] Mordvintsev DY, Rodionov DI, Makarova MV, Kamensky AA, Levitskaya NG, Ogay AY, Rzhevsky DI, Murashev AN, Tsetlin VI, Utkin YN. Behavioural effects in mice and intoxication symptomatology of weak neurotoxin from cobra Naja kaouthia. Ba‐

[43] Mordvintsev DY, Polyak YL, Rodionov DI, Jakubik J, Dolezal V, Karlsson E, Tsetlin VI, Utkin YN. Weak toxin WTX from Naja kaouthia cobra venom interacts with both nicotinic and muscarinic acetylcholine receptors. FEBS Journal 2009;276(18)

Journal of Biological Chemistry 2006;281(39) 29030-29041.

sic and Clinical Pharmacology and Toxicology 2007;100(4) 273-278.

line receptors. Journal of Biological Chemistry 2012;287(9) 6725-6734.

ropean Journal of Biochemistry 2000;267(23) 6784-6789.

2008;283(21) 14571-14580.

134 Neurochemistry

15810-15815.

17811-17820.

5065-5075.


targets: Exploration of green mamba venom for novel drug candidates active against adrenoceptors. Toxicon 2012;59(4) 487-496.

[65] Schmidt JJ, Weinstein SA, Smith LA. Molecular properties and structure-function re‐ lationships of lethal peptides from venom of Wagler's pit viper, Trimeresurus wa‐

Peptide and Protein Neurotoxin Toolbox in Research on Nicotinic Acetylcholine Receptors

http://dx.doi.org/10.5772/58240

137

[66] Tsai MC, Hsieh WH, Smith LA, Lee CY. Effects of waglerin-I on neuromuscular transmission of mouse nerve-muscle preparations. Toxicon 1995;33(3) 363-371.

[67] McArdle JJ, Lentz TL, Witzemann V, Schwarz H, Weinstein SA, Schmidt JJ. Wagler‐ in-1 selectively blocks the epsilon form of the muscle nicotinic acetylcholine receptor.

[68] Molles BE, Rezai P, Kline EF, McArdle JJ, Sine SM, Taylor P. Identification of resi‐ dues at the α and ε subunit interfaces mediating species selectivity of Waglerin-1 for nicotinic acetylcholine receptors. Journal of Biological Chemistry 2002;277(7)

[69] Molles BE, Tsigelny I, Nguyen PD, Gao SX, Sine SM, Taylor P. Residues in the ε sub‐ unit of the nicotinic acetylcholine receptor interact to confer selectivity of waglerin-1

[70] Schmidt JJ, Weinstein SA. Structure-function studies of waglerin I, a lethal peptide from the venom of Wagler's pit viper, Trimeresurus wagleri. Toxicon 1995;33(8)

[71] Utkin YN, Weise C, Kasheverov IE, Andreeva TV, Kryukova EV, Zhmak MN, Star‐ kov VG, Hoang NA, Bertrand D, Ramerstorfer J, Sieghart W, Thompson AJ, Lummis SC, Tsetlin VI. Azemiopsin from Azemiops feae viper venom, a novel polypeptide li‐ gand of nicotinic acetylcholine receptor. Journal of Biological Chemistry 2012;287(32)

[72] Gray WR, Luque A, Olivera BM, Barrett J, Cruz LJ. Peptide toxins from Conus geo‐

[73] Halai R, Craik DJ. Conotoxins: natural product drug leads. Natural Product Reports

[74] Teichert RW, Olivera BM. Natural products and ion channel pharmacology. Future

[75] Lewis RJ, Dutertre S, Vetter I, Christie MJ. Conus venom peptide pharmacology.

[76] Kreienkamp HJ, Sine SM, Maeda RK, Taylor P. Glycosylation sites selectively inter‐ fere with α-toxin binding to the nicotinic acetylcholine receptor. Journal of Biological

[77] Groebe DR, Dumm JM, Levitan ES, Abramson SN. α-Conotoxins selectively inhibit one of the two acetylcholine binding sites of nicotinic receptors. Molecular Pharma‐

graphus venom. Journal of Biological Chemistry 1981;256(10) 4734-4740.

for the α-ε subunit interface site. Biochemistry 2002;41(25) 7895-7906.

Journal of Pharmacology and Experimental Therapeutics 1999;289(1) 543-550.

gleri. Toxicon 1992;30(9) 1027-1036.

5433-5440.

1043-1049.

27079-27086.

2009;26(4) 526-536.

Medicinal Chemistry 2010;2(5) 731-444.

Chemistry 1994;269(11) 8108-8114.

cology 1995;48(1) 105-111.

Pharmacological Reviews 2012;64(2) 259-298.


[65] Schmidt JJ, Weinstein SA, Smith LA. Molecular properties and structure-function re‐ lationships of lethal peptides from venom of Wagler's pit viper, Trimeresurus wa‐ gleri. Toxicon 1992;30(9) 1027-1036.

targets: Exploration of green mamba venom for novel drug candidates active against

[56] Miwa JM, Ibanez-Tallon I, Crabtree GW, Sánchez R, Sali A, Role LW, Heintz N. Lynx1, an endogenous toxin-like modulator of nicotinic acetylcholine receptors in

[57] Adermann K, Wattler F, Wattler S, Heine G, Meyer M, Forssmann WG, Nehls M. Structural and phylogenetic characterization of human SLURP-1, the first secreted mammalian member of the Ly-6/uPAR protein superfamily. Protein Science 1999;8(4)

[58] Miwa JM, Stevens TR, King SL, Caldarone BJ, Ibanez-Tallon I, Xiao C, Fitzsimonds RM, Pavlides C, Lester HA, Picciotto MR, Heintz N. The prototoxin lynx1 acts on nic‐ otinic acetylcholine receptors to balance neuronal activity and survival in vivo. Neu‐

[59] Tekinay AB, Nong Y, Miwa JM, Lieberam I, Ibanez-Tallon I, Greengard P, Heintz N. A role for LYNX2 in anxiety-related behavior. Proc Natl Acad Sci U S A. 2009 Mar

[60] Lyukmanova EN, Shenkarev ZO, Shulepko MA, Mineev KS, D'Hoedt D, Kasheverov IE, Filkin SY, Krivolapova AP, Janickova H, Dolezal V, Dolgikh DA, Arseniev AS, Bertrand D, Tsetlin VI, Kirpichnikov MP. NMR structure and action on nicotinic ace‐ tylcholine receptors of water-soluble domain of human LYNX1. Journal of Biological

[61] Shulepko MA, Lyukmanova EN, Paramonov AS, Lobas AA, Shenkarev ZO, Kashe‐ verov IE, Dolgikh DA, Tsetlin VI, Arseniev AS, Kirpichnikov MP. Human neuromo‐ dulator SLURP-1: bacterial expression, binding to muscle-type nicotinic acetylcholine receptor, secondary structure, and conformational heterogeneity in solution. Bio‐

[62] Lyukmanova EN, Shulepko MA, Buldakova SL, Kasheverov IE, Shenkarev ZO, Re‐ shetnikov RV, Filkin SY, Kudryavtsev DS, Ojomoko LO, Kryukova EV, Dolgikh DA, Kirpichnikov MP, Bregestovski PD, Tsetlin VI. Water-soluble LYNX1 residues im‐ portant for interaction with muscle-type and/or neuronal nicotinic receptors. Journal

[63] Thomsen MS, Cinar B, Jensen MM, Lyukmanova EN, Shulepko MA, Tsetlin V, Klein AB, Mikkelsen JD. Expression of the Ly-6 family proteins Lynx1 and Ly6H in the rat brain is compartmentalized, cell-type specific, and developmentally regulated. Brain

[64] Weinstein SA, Schmidt JJ, Bernheimer AW, Smith LA. Characterization and amino acid sequences of two lethal peptides isolated from venom of Wagler's pit viper, Tri‐

adrenoceptors. Toxicon 2012;59(4) 487-496.

810-819.

136 Neurochemistry

ron 2006;51(5) 587-600.

17;106(11):4477-82.

Chemistry 2011;286(12) 10618-10627.

chemistry (Moscow) 2013;78(2) 204-211.

of Biological Chemistry 2013;288(22) 15888-15899.

meresurus wagleri. Toxicon 1991;29(2) 227-236.

Structure and Function 2013. doi 10.1007/s00429-013-0611-x.

the mammalian CNS. Neuron 1999;23(1) 105-114.


[78] Utkin YN, Kobayashi Y, Hucho F, Tsetlin VI. Relationship between the binding sites for an α-conotoxin and snake venom neurotoxins in the nicotinic acetylcholine recep‐ tor from Torpedo californica. Toxicon 1994;32(9) 1153-1157.

[90] Kasheverov IE, Utkin YN, Tsetlin VI. Naturally occurring and synthetic peptides act‐ ing on nicotinic acetylcholine receptors. Current Pharmaceutical Design 2009;15(21)

Peptide and Protein Neurotoxin Toolbox in Research on Nicotinic Acetylcholine Receptors

http://dx.doi.org/10.5772/58240

139

[91] Armishaw CJ. Synthetic α-conotoxin mutants as probes for studying nicotinic acetyl‐ choline receptors and in the development of novel drug leads. Toxins (Basel)

[92] Muttenthaler M, Akondi KB, Alewood PF. Structure-activity studies on α-conotox‐

[93] Smit AB, Syed NI, Schaap D, van Minnen J, Klumperman J, Kits KS, Lodder H, van der Schors RC, van Elk R, Sorgedrager B, Brejc K, Sixma TK, Geraerts WP. A gliaderived acetylcholine-binding protein that modulates synaptic transmission. Nature

[94] Celie PH, van Rossum-Fikkert SE, van Dijk WJ, Brejc K, Smit AB, Sixma TK. Nicotine and carbamylcholine binding to nicotinic acetylcholine receptors as studied in

[95] Ulens C, Akdemir A, Jongejan A, van Elk R, Bertrand S, Perrakis A, Leurs R, Smit AB, Sixma TK, Bertrand D, de Esch IJ. Use of acetylcholine binding protein in the search for novel α7 nicotinic receptor ligands. In silico docking, pharmacological screening, and X-ray analysis. Journal of Medicinal Chemistry 2009;52(8) 2372-2383.

[96] Hibbs RE, Sulzenbacher G, Shi J, Talley TT, Conrod S, Kem WR, Taylor P, Marchot P, Bourne Y. Structural determinants for interaction of partial agonists with acetylcho‐ line binding protein and neuronal α7 nicotinic acetylcholine receptor. EMBO Journal

[97] Akdemir A, Rucktooa P, Jongejan A, Elk Rv, Bertrand S, Sixma TK, Bertrand D, Smit AB, Leurs R, de Graaf C, de Esch IJ. Acetylcholine binding protein (AChBP) as tem‐ plate for hierarchical in silico screening procedures to identify structurally novel li‐ gands for the nicotinic receptors. Bioorganic and Medicinal Chemistry 2011;19(20)

[98] Rucktooa P, Smit AB, Sixma TK. Insight in nAChR subtype selectivity from AChBP

[99] Yakel JL. Gating of nicotinic ACh receptors: latest insights into ligand binding and

[100] [100] Hucho F, Tsetlin VI, Machold J. The emerging three-dimensional structure of a receptor. The nicotinic acetylcholine receptor. European Journal of Biochemistry

[101] Fruchart-Gaillard C, Gilquin B, Antil-Delbeke S, Le Novere N, Tamiya T, Corringer PJ, Changeux JP, Menez A, Servent D. Experimentally based model of a complex be‐

crystal structures. Biochemical Pharmacology 2009;78(7) 777-787.

function. Journal of Physiology 2010;588(Pt 4) 597-602.

ins. Current Pharmaceutical Design 2011;17(38) 4226-4241.

AChBP crystal structures. Neuron 2004;41(6) 907-914.

2430-2452.

2010;2(6) 1471-1499.

2001;411(6835) 261-268.

2009;28(19) 3040-3051.

1996;239(3) 539-557.

6107-6119.


[90] Kasheverov IE, Utkin YN, Tsetlin VI. Naturally occurring and synthetic peptides act‐ ing on nicotinic acetylcholine receptors. Current Pharmaceutical Design 2009;15(21) 2430-2452.

[78] Utkin YN, Kobayashi Y, Hucho F, Tsetlin VI. Relationship between the binding sites for an α-conotoxin and snake venom neurotoxins in the nicotinic acetylcholine recep‐

[79] Hann RM, Pagán OR, Eterović VA. The α-conotoxins GI and MI distinguish between the nicotinic acetylcholine receptor agonist sites while SI does not. Biochemistry

[80] Groebe DR, Gray WR, Abramson SN. Determinants involved in the affinity of α-con‐ otoxins GI and SI for the muscle subtype of nicotinic acetylcholine receptors. Bio‐

[81] Hann RM, Pagan OR, Gregory LM, Jacome T, Eterovic VA. The 9-arginine residue of α-conotoxin GI is responsible for its selective high affinity for the αγ agonist site on

[82] Jacobsen RB, DelaCruz RG, Grose JH, McIntosh JM, Yoshikami D, Olivera BM. Criti‐ cal residues influence the affinity and selectivity of α-conotoxin MI for nicotinic ace‐

[83] Ellison M, Feng ZP, Park AJ, Zhang X, Olivera BM, McIntosh JM, Norton RS. α-RgIA, a novel conotoxin that blocks the α9α10 nAChR: structure and identification of key receptor-binding residues. Journal of Molecular Biology 2008;377(4) 1216-1227.

[84] Servent D, Thanh HL, Antil S, Bertrand D, Corringer PJ, Changeux JP, Ménez A. Functional determinants by which snake and cone snail toxins block the α7 neuronal nicotinic acetylcholine receptors. Journal of Physiology-Paris 1998;92(2) 107-111. [85] Hogg RC, Hopping G, Alewood PF, Adams DJ, Bertrand D. α-Conotoxins PnIA and [A10L]PnIA stabilize different states of the α7-L247T nicotinic acetylcholine receptor.

[86] Everhart D, Cartier GE, Malhotra A, Gomes AV, McIntosh JM, Luetje CW. Determi‐ nants of potency on α-conotoxin MII, a peptide antagonist of neuronal nicotinic re‐

[87] Millard EL, Nevin ST, Loughnan ML, Nicke A, Clark RJ, Alewood PF, Lewis RJ, Adams DJ, Craik DJ, Daly NL. Inhibition of neuronal nicotinic acetylcholine receptor subtypes by α-conotoxin GID and analogues. Journal of Biological Chemistry

[88] Halai R, Clark RJ, Nevin ST, Jensen JE, Adams DJ, Craik DJ. Scanning mutagenesis of α-conotoxin Vc1.1 reveals residues crucial for activity at the α9α10 nicotinic acetyl‐

[89] Grishin AA, Cuny H, Hung A, Clark RJ, Brust A, Akondi K, Alewood PF, Craik DJ, Adams DJ. Identifying key amino acid residues that affect α-conotoxin AuIB inhibi‐ tion of α3β4 nicotinic acetylcholine receptors. Journal of Biological Chemistry 2013.

choline receptor. Journal of Biological Chemistry 2009;284(30) 20275-20284.

the electric organ acetylcholine receptor. Biochemistry 1997;36(29) 9051-9056.

tor from Torpedo californica. Toxicon 1994;32(9) 1153-1157.

tylcholine receptors. Biochemistry 1999;38(40) 13310-13315.

Journal of Biological Chemistry 2003;278(29) 26908-26914.

ceptors. Biochemistry 2004;43(10) 2732-2737.

2009;284(8) 4944-4951.

doi: 10.1074/jbc.M113.512582.

1994;33(47) 14058-14063.

138 Neurochemistry

chemistry 1997;36(21) 6469-6474.


tween a snake toxin and the α7 nicotinic receptor. Proceedings of the National Acad‐ emy of Sciences of the USA 2002;99(5) 3216-3221.

[113] Brams M, Pandya A, Kuzmin D, van Elk R, Krijnen L, Yakel JL, Tsetlin V, Smit AB, Ulens C. A structural and mutagenic blueprint for molecular recognition of strych‐ nine and d-tubocurarine by different cys-loop receptors. PLoS Biology 2011;9(3)

Peptide and Protein Neurotoxin Toolbox in Research on Nicotinic Acetylcholine Receptors

http://dx.doi.org/10.5772/58240

141

[114] Hansen SB, Sulzenbacher G, Huxford T, Marchot P, Taylor P, Bourne Y. Structures of Aplysia AChBP complexes with nicotinic agonists and antagonists reveal distinctive

[115] Ulens C, Hogg RC, Celie PH, Bertrand D, Tsetlin V, Smit AB, Sixma TK. Structural determinants of selective α-conotoxin binding to a nicotinic acetylcholine receptor homolog AChBP. Proceedings of the National Academy of Sciences of the USA

[116] Dutertre S, Ulens C, Büttner R, Fish A, van Elk R, Kendel Y, Hopping G, Alewood PF, Schroeder C, Nicke A, Smit AB, Sixma TK, Lewis RJ. AChBP-targeted α-conotox‐ in correlates distinct binding orientations with nAChR subtype selectivity. EMBO

[117] Talley TT, Reger AS, Kim C, Sankaran B, Ho K, Taylor P, McIntosh J.M. Crystal struc‐ ture of acetylcholine binding protein (AChBP) from Aplysia Californica in complex

[118] Kasheverov IE, Chiara DC, Zhmak MN, Maslennikov IV, Pashkov VS, Arseniev AS, Utkin YN, Cohen JB, Tsetlin VI. α-Conotoxin GI benzoylphenylalanine derivatives.

[119] Srivastava S, Hamouda AK, Pandhare A, Duddempudi PK, Sanghvi M, Cohen JB, Blanton MP. [(3)H]Epibatidine photolabels non-equivalent amino acids in the agonist binding site of Torpedo and α4β2 nicotinic acetylcholine receptors. Journal of Biolog‐

H-NMR structures and photoaffinity labeling of the Torpedo californica nicotinic

with α-conotoxin BuIA. 2013. doi: 10.2210/pdb4ez1/pdb.

acetylcholine receptor. FEBS Journal 2006;273(7) 1373-1388.

ical Chemistry 2009;284(37) 24939-24947.

binding interfaces and conformations. EMBO Journal 2005;24(20) 3635-46.

e1001034.

1

2006;103(10) 3615-3620.

Journal 2007;26(16) 3858-3867.


[113] Brams M, Pandya A, Kuzmin D, van Elk R, Krijnen L, Yakel JL, Tsetlin V, Smit AB, Ulens C. A structural and mutagenic blueprint for molecular recognition of strych‐ nine and d-tubocurarine by different cys-loop receptors. PLoS Biology 2011;9(3) e1001034.

tween a snake toxin and the α7 nicotinic receptor. Proceedings of the National Acad‐

[102] Tsetlin VI, Hucho F. Snake and snail toxins acting on nicotinic acetylcholine recep‐ tors: fundamental aspects and medical applications. FEBS Letters 2004;557(1-3) 9-13.

[103] Gershoni JM, Hawrot E, Lentz TL. Binding of α-bungarotoxin to isolated α subunit of the acetylcholine receptor of Torpedo californica: quantitative analysis with protein blots. Proceedings of the National Academy of Sciences of the USA 1983;80(16)

[104] Schrattenholz A, Pfeiffer S, Pejovic V, Rudolph R, Godovac-Zimmermann J, Maelicke A. Expression and renaturation of the N-terminal extracellular domain of Torpedo nicotinic acetylcholine receptor α-subunit. Journal of Biological Chemistry

[105] Tsetlin VI, Dergousova NI, Azeeva EA, Kryukova EV, Kudelina IA, Shibanova ED, Kasheverov IE, Methfessel C. Refolding of the Escherichia coli expressed extracellu‐ lar domain of α7 nicotinic acetylcholine receptor (Cys116 mutation diminishes aggre‐ gation and stabilizes the β structure). European Journal of Biochemistry 2002;269(11)

[106] Zouridakis M, Zisimopoulou P, Eliopoulos E, Poulas K, Tzartos SJ. Design and ex‐ pression of human α7 nicotinic acetylcholine receptor extracellular domain mutants with enhanced solubility and ligand-binding properties. Biochimica et Biophysica

[107] Li SX, Huang S, Bren N, Noridomi K, Dellisanti CD, Sine SM, Chen L. Ligand-bind‐ ing domain of an α7-nicotinic receptor chimera and its complex with agonist. Nature

[108] Dukat M, Glennon RA. Epibatidine: impact on nicotinic receptor research. Cellular

[109] Celie PH, Kasheverov IE, Mordvintsev DY, Hogg RC, van Nierop P, van Elk R, van Rossum-Fikkert SE, Zhmak MN, Bertrand D, Tsetlin V, Sixma TK, Smit AB. Crystal structure of nicotinic acetylcholine receptor homolog AChBP in complex with an αconotoxin PnIA variant. Nature Structural and Molecular Biology 2005;12(7) 582-588.

[110] Hansen SB, Talley TT, Radic Z, Taylor P. Structural and ligand recognition character‐ istics of an acetylcholine-binding protein from Aplysia californica. Journal of Biologi‐

[111] Dutertre S, Lewis RJ. Toxin insights into nicotinic acetylcholine receptors. Biochemi‐

[112] Tsetlin V, Utkin Y, Kasheverov I. Polypeptide and peptide toxins, magnifying lenses for binding sites in nicotinic acetylcholine receptors. Biochemical Pharmacology

emy of Sciences of the USA 2002;99(5) 3216-3221.

4973-4977.

140 Neurochemistry

2801-2809.

1998;273(49) 32393-32399.

Acta 2009;1794(2) 355-366.

Neuroscience 2011;14(10) 1253-1259.

cal Chemistry 2004;279(23) 24197-24202.

cal Pharmacology 2006;72(6) 661-670.

2009;78(7) 720-731.

and Molecular Neurobiology 2003;23(3) 365-378.


**Chapter 5**

**Synaptic Soluble and Membrane-Bound Choline**

**Vitro and In Vivo**

http://dx.doi.org/10.5772/58307

**1. Introduction**

E.I. Zakharova and A.M. Dudchenko

Additional information is available at the end of the chapter

**1.1. Synaptosomes — Definition, a bit of history**

first isolated and identified nerve endings in nervous tissue [2].

**Acetyltransferase as a Marker of Cholinergic Function In**

The synaptosome is the presynaptic part of the nerve ending with, as a rulе, a postsynaptic membrane in the region of the junction of the pre- and postsynaptic membranes that remains with the presynapse during homogenization and centrifugation. The presynaptic part of the synaptosome is a membrane-bound structure with a preserved cytoplasm (synaptoplasm), synaptic vesicles, mitochondria and some other cellular components. The term *synaptosome* was adopted by V.P. Whittaker and coworkers [1]. Together, V.P. Whittaker and C.O. Hebb

Subcellular fractionation emerged in the 1930s and 1940s and has since established itself as a major technique in experimental biology. The first attempts at the fractionation of nervous tissue were made in the early 1950s. A few years later, the fraction of synaptosomes was successful, using discontinuous sucrose-density gradient centrifugation [2-4]. After this, researchers achieved the preparation of the synaptic components, including the synaptic membranes, synaptoplasm, synaptic vesicles [1, 5, 6] (Figure 1) and membrane junction complex [7, 8]. These studies were a powerful impetus for investigations into the biochemistry of synapses and in the development of new methods of synaptic fractionation. Synaptosomes, as nerve endings, are heterogeneous in density, size and mediator specificity. Therefore, a number of the methods were developed for separating the synaptosomal fraction into two fractions [4], as well as into many fractions using a continuous sucrose-density linear gradient [9-11]. Among the many publications at this time, two books stand out. In the first one, D.J. Jones recounts the story of subcellular fractionation techniques, and presents the entire set of

> © 2014 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
