**2.8. Lipids profile analysis**

Total cholesterol, triglyceride, high density lipoprotein cholesterol, low density lipoprotein cholesterol were assayed using the method of Zlakis *et al.* [28], Foster and Dunn [29], Burstein *et al.*[30] and Friedwald *et al.* [31] respectively.

#### **2.9. Determination of liver function indices**

The procedure described by Doumas *et al.* [32] and Sherlock [33] was used for the determina‐ tion of albumin and bilirubin concentrations respectively.

The method of Tietz *et al*. [34] was used to determine the levels of serum urea and creatinine.

#### **2.10. Determination of superoxide dismutase activity and malondialdehyde concentration**

The pancreatic superoxide dismutase activity was determined by the method of Misra and Fridovich [35] while malondialdehyde concentration was assayed using the method described by Varshney and Kale [36].

#### **2.11. Statistical analysis**

All data are expressed as the mean of sixth replicates ± standard error of mean (S.E.M). Statistical evaluation of data was performed by SPSS version 16 using one way analysis of variance (ANOVA), followed by Dunett's posthoc test for multiple comparism. Values were considered statistically significant at p<0.05 (confidence level = 95%).
