**12. Potential application of HSM**

The HSM that we developed can be safely used for the elimination of hiPS cells because it does not contain hazardous reagents or introduce genetic material. Our results show that hiPS cells die after three days of culture in HSM. Prior to performing the experiments, we compared the hiPS cell viability in media containing crude or dialyzed KSR or combination of insulin (10 µM), dexamethasone (10 µM) and aprotitin (5000 U/ml) (IDA) Unexpectedly, the KSR dialysis and IDA had no effect on hiPS cell survival. As expected, primary human hepatocytes survived in HSM as well as in HCM, which is the recommended medium for their culture.

HSM can be used in clinical practices in situations when hepatocytes differentiated from human iPS cells are transplanted to patients suffering from liver failure.

**Figure 5.** Human iPS cells co-cultured with primary human hepatocytes in HSM. Scale bar, 50 μm; arrow, hepatocytes; arrowhead, 201B7 cells.
