**4. Conclusion**

The in situ single cell cutting experiment was performed using these three nano knives. Figure 5(A) shows the initialposition ofthe nano knife anda single cell. Figure 5(B) shows the touching between the nano knife tip and the single cell. The deformation of the nano knife beam and the single cell during the cutting is shown in Figure 5(C). The deformation of the beam can be measured from the ESEM image directly using image analysis software. Therefore, the cutting force can be calculated based on Hooke's law. The separated single cell after cutting is shown in Figure 5(D). The sample slice angle can be measured from the ESEM image directly as well. Figure 5(E) shows the single cell cutting image using the 25o knife. Figure 5(F) shows the sample slice angle after cutting. The images of single cell cutting and sample slice angle after cutting

Micro-Nano Mechatronics — New Trends in Material, Measurement, Control, Manufacturing and Their Applications in

using 45o knife are shown in Figure 5 (G) and Figure 5 (H).

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**Figure 4.** Schematic drawing of the single cell cutting experiment using nano knife inside ESEM.

**Figure 5.** Single cell cutting using nano knife inside ESEM.

This chapter presents the single cell nanosurgery system based on nanomanipulation techni‐ ques.The micro‐nano tools have been proposed to investigate single cell analysis to manipulate and control the local environment in micro‐nano scale. The E‐SEM nanomanipulation system was constructed to realize the local stiffness evaluation, local cutting, and local extraction of biological organism in nano‐meter scale. The adhesion force measurement was presented by micro‐putter for single cells. The single cell cutting was also described using nano‐knife. As future direction, the multiple micro‐nanotools are used continuously depending on the purposes by exchanging machinery system (NTExS: Nanotool Exchanger System) [39]. We are investigating on the nanoinjection applications for the Caenorhabditis elegans (C. elegans) as one of the model organisms [40, 41]
