**3.5 Interspecies crosses**

360 Gene Duplication

Fig. 3. Log of Rm versus gel concentrations relationship (Ferguson's plot) for EST-4 and EST-5. A – *Drosophila mulleri*; B – *Drosophila aldrichi*; C – *Drosophila wheeleri*; D – *Drosophila navojoa*.

*cluster* Slope M.W. Slope M.W.  *D. mulleri* –10.52607 ± 0.44878 88.218 ± 4.748 –11.03429 ± 0.30178 93.595 ± 3.193  *D. aldrichi* –10.29768 ± 0.30168 85.802 ± 3.192 –10.34904 ± 0.28362 86.346 ± 3.000  *D. wheeleri* –10.08361 ± 0.33581 83.537 ± 3.553 –10.32114 ± 0.30252 86.050 ± 3.201

*1D. mojavensis* –10.45339 ± 0.27581 87.450 ± 2.918 –10.33036 ± 0.27437 86.148 ± 2.903 *1D. arizonae* –10.27775 ± 0.32146 85.591 ± 3.401 –10.16554 ± 0.30570 84.404 ± 3.234  *D. navojoa* –10.24157 ± 0.38515 85.209 ± 4.074 –10.05407 ± 0.29546 83.225 ± 3.126

Table 4. Slopes of Log Rm versus gel concentration relationships and MW estimates for EST-4 and EST-5 of the six cactophilic *Drosophila* species analyzed.1 Data obtained from Mateus et

*D. mulleri* 

*D. mojavensis cluster* 

al. (2009).

**EST-4 EST-5** 
