**8. Results**

112 Gastrointestinal Endoscopy

polymerase in PCR buffer 1X. Real-time PCR analysis was performed with an Rotor-Gene 6000 (Corbett Research, Australia). The conditions of PCR amplification were 95°C for 5 min and 45 cycles of 95°C for 30 s and 58°C for 40 s. All samples were repeated twice and positive and negative controls were enclosed in each assay. Data were analyzed with rotorgene 6000 software *ver1.7* (Corbett research). In order to test the specificity of the primers

In order to made standard serial dilution, standard density of bacteria was prepared. After DNA extraction, one 10-fold serial dilution of *H. pylori* DNA was made, with bacterial concentrations ranging from 5×102 to 5×107 bacteria per 1 µl. Bacterial quantification was

Statistical analyses were conducted with *chi-square* using the SPSS for Windows (version 17; SPSS, Inc., Chicago, Illinois, USA). *P*-values less than 0.05 were taken to indicate statistical

Target Sequence (5′→3′) Primer/Probe

AAGCTTATTTCTAACGC HP-2 *ureC* 

TTCCCGCTTAGATGCTTTCAG Cla-21 *23S rRNA* 

CTCCATAAGAGCCAAAGCCC HP23S-2 *23S rRNA*  Wild type Cy5-GGGGTCTTTCCGTCT-BHQ2 Pwt A2144G TAMRA-GGTCCTTCCGTCTTG-Dabcyl P44G A2143G TET-GGTCTCTCCGTCTTG-Dabcyl P43G A2143C HEX-GGTCTGTCCGTCTTG-Dabcyl P43C A2142G FAM-GGTCTTCCCGTCTTG-Dabcyl P42G

Table 4. Oligonucleotides sequence used in this study

 Vomiting Flatulence Acid Reflux Nausea Early Satiety Heaviness after Meal Anorexia Pain

Fig. 7. Prevalence of patients reported symptoms in analysis 1

AAGCTTTTAGGGGTGTTAGGGGTTT HP-1

AGTCGGGACCTAAGGCGAG Cla-18

CCACAGCGATGTGGTCTCAG HP23S-1

0 20 40 60 80

purified DNA of non-*H. pylori* strains was also used as a template for PCR.

performed by TaqMan probe technology of real-time PCR as described above.

**Quantification real-time PCR** 

**Statistical analysis** 

significance.
