**" Grappolo "**

*(synonymy: Frantoie a grappoli)* 

Areal distribution or origin area: **Toscana** Flesh/pit weight ratio: **medium (6,01 ± 0,47)**  Oil content (%): **medium (47,30 ± 1,64)** Purpose: **oil**

## *Morphological characters*

*Tree characters*  Vigour: **medium**  Growth habit: **spreading**  Canopy-density: **medium-dense** 

*Biochemical Characters*

146 Olive Germplasm – Italian Catalogue of Olive Varieties

Myristic acid **0,02 ± 0,01**  Palmitic acid **13,78 ± 1,53** Palmitoleic acid **1,10 ± 0,03** Stearic acid **2,20 ± 0,14** Oleic acid **63,94 ± 1,90**

*Fatty Acid Composition* 

*Organoleptic oil values* 

Sensory Analysis (*Panel test*)

*Molecular Markers* 

**References:** 

*Comment:* **fruity medium-high, with hints of almond and read sensations of grass and artichoke. Balanced taste sensation with a medium bitter and spicy. Medium fluidity.**

*Table 2.* Microsatellites (SSR) *loci* analyzed.

Linoleic acid (ǚ6) **16,40 ± 2,45** Linolenic acid (ǚ3) **0,95 ± 0,07** Arachic acid **0,24 ± 0,05** Eicosenoic acid **0,15 ± 0,22** Behenic acid **0,08 ± 0,02**

*DCA09 DCA18 GAPU59 GAPU71A GUPA71B GAPU103A*  **162 - 206 175 - 175 214 - 214 210 - 214 124 - 144 170 - 170** 

*UDO01 UDO03 UDO12 UDO28 UDO39*  **144 - 144 150 - 182 166 - 166 154 - 182 220 - 243** 

2 - Muzzalupo I., Salimonti A., Caravita M. A., *et al. Adv. Hort. Sci*. (2008), 22(2): pp. 129-135.

1 - Macrì T., Picone G., La Porta G. In : *Olivo e Olio,* (1988), 5: pp. 34-41.

For each *locus* the allele size (expressed in base pairs) is reported.

*Table 1.* Average values (express in % ± standard deviations) of the fatty acids methyl esters and nutritional ratios obtained from single cultivar olive oils.

Lignoceric acid **0,04 ± 0,03** 

Unsat./satured **5,14 ± 0,62** ǚ6/ǚ3 **17,40 ± 3,01**

> *Leaf characters* Blade length (cm): **medium (5,60 ± 0,46)** Blade width (cm): **broad (1,59 ± 0,12)** Shape (length/width): **elliptic**

*Inflorescence characters* Inflorescence length (cm): **medium (2,95 ± 0,35)** Number of flowers: **low (17,73 ± 1,82)**

*Fruit characters* Fresh weight of 100 fruits (g): **medium (2,59 ± 0,06)** Shape (length/width): **spherical**  Symmetry: **symmetric**  Position of maximum transverse diameter: **central**  Apex: **rounded**  Base: **truncate**  Niplle: **absent** Lenticels: **many and small** 

*Pit characters*  Weight of 100 pits (g): **medium (0,37 ± 0,02)** Shape (length/width): **ovoid**  Mucron: **obvious**  Symmetry: **slightly asymmetric**  Position of maximum transverse diameter: **towards apex**  Apex: **rounded**  Base: **rounded**  Surface: **smooth**  Number of grooves: **medium** 

### *Fatty Acid Composition*

**" Grignan "** *(synonymy: Brescian, Gargnano, Gregnara, etc.)*  Innocenzo Muzzalupo 149

Areal distribution or origin area: **Veneto** Flesh/pit weight ratio: **low (4,90 ± 1,25)**  Oil content (%): **medium (40,00 ± 0,62)**

*Morphological characters* 

Purpose: **oil**

*Tree characters*  Vigour: **medium** 

*Leaf characters*

*Fruit characters*

**central**  Apex: **rounded**  Base: **truncate**  Niplle: **tenuous**

*Inflorescence characters*

Growth habit: **erect-spreading**  Canopy-density: **medium** 

Blade length (cm): **medium (5,66 ± 0,59)** Blade width (cm): **medium (1,41 ± 0,19)** Shape (length/width): **elliptic-lanceolate** 

Number of flowers: **low (14,58 ± 0,67)**

Shape (length/width): **spherical**  Symmetry: **slightly asymmetric** 

Lenticels: **few and small** 

Shape (length/width): **ovoid** 

Symmetry: **slightly asymmetric** 

*Pit characters* 

Mucron: **obvious** 

**central**  Apex: **rounded**  Base: **rounded**  Surface: **scabrous**  Number of grooves: **high** 

Inflorescence length (cm): **medium (2,77 ± 0,21)**

Fresh weight of 100 fruits (g): **medium (3,31 ± 0,06)**

Position of maximum transverse diameter:

Weight of 100 pits (g): **high (0,60 ± 0,15)**

Position of maximum transverse diameter:

*Table 1.* Average values (express in % ± standard deviations) of the fatty acids methyl esters and nutritional ratios obtained from single cultivar olive oils.


### *Organoleptic oil values*

Sensory Analysis (*Panel test*)

*Comment:* **fruity medium - light, with hints of artichoke and almond. Balanced in flavours, with hints of bitter and spicy medium intensity, medium fluidity.**

## *Molecular Markers*

*Table 2.* Microsatellites (SSR) *loci* analyzed.

For each *locus* the allele size (expressed in base pairs) is reported.


#### **References:**

1 - Cimato A., Cantini C., Sani G., In: *L'olivo in Toscana: il germoplasma autoctono*, Ed. ARSIA (2001).

2 - Various authors. In: *Catalogo Nazionale delle Varietà di Olivo*, University of Bari (in press), ISBN 978-88-88793- 97-9.
