**4.2.1.3 Evaluation of blood compatibility**

In order to investigate the complement and immunoglobin activation, complement C3, C4 and immunoglobin G, A, M and E were determined by enzyme-linked immunosorbent assays (ELISA)

Blood cells including red blood cell (RBC) and white blood cell (WBC), and blood components including hemoglobin (HGB) and platelet were determined using a blood cell analyzer (BC-3000peus, Shenzhen Mairui Biomedical Device Co. Ltd., China). Blood gas was determined by a blood gas analyzer (CORNing 238, US).
