**4.1.1.1 Hemodialysis procedure**

Adult hybrid goats (about 20 kg) were used in the experiment. All the animals underwent local anesthesia with 1.0% procaine hydrochloride by injection into the neck muscle. The hair on the neck was cleared away carefully. The animal was laid on its back and fixed on the experimental table.

Extracorporeal circuits were primed with 500ml normal saline solution to remove the bubbles in the circuits and in the dialyzer, then primed with 500ml saline solution containing 10000 IU heparin. 150mg urea and 50mg creatinine were injected to the animal blood before the treatment. At the initiation of the treatment goats received a loading dose (3000 IU) of heparin, and followed by continuous infusion (3000 IU/h). The infusion was terminated at 30 min prior to the end of the dialysis.
