**1. Introduction**

Chromatography is the separation of a mixture of compounds into its individual components based on their relative interactions with an inert matrix. However, chromatography is more than a simple technique, it is an important part of science encompassing chemistry, physical chemistry, chemical engineering, biochemistry and cutting through different fields. It is worth to be mentioned here that the IUPAC definition of chromatography is "separation of sample components after their distribution between two phases".
