**Author details**

**Bioactive compound Molecular structure Target protein**

N

O O

N

H

H

H HO

O O

N

O

O

O

O O O

**Table 6.** Selected examples of the target protein(s) isolated and identified through affinity chromatography [30]

Liquid chromatographic techniques have been widely used in pharmaceutical and clinical laboratories. Reversed phase, ion exchange, size exclusion and normal phase chromatogra‐

N

OH

O

O

O

N

NH2

N

NH2

OCH3

Dihydrofolate reductase Deoxycytidine kinase

Tif1 (yeast elF4A)

Splicing factor 3b

Cereblon

Polo-like kinase 1 Polo-

like kinase 3

N

N

HO

N

CH3O

O

<sup>O</sup> <sup>O</sup>

OH

HO

Methotrexate (anticancer drug)

94 Column Chromatography

Resvertatrol (health benefits including life span)

Spliceostatin A (splicing inhibitor)

Thalidomide (sedative, anticancer

Wortmannin

**4. Conclusion**

drug)

HO

O

AcO

(PI 3-kinase inhibitor) <sup>O</sup>

Özlem Bahadir Acikara, Gülçin Saltan Çitoğlu, Serkan Özbilgin and Burçin Ergene

Ankara University, Faculty of Pharmacy, Department of Pharmacognosy, Ankara, Turkey
