**2.3. Methylation**

A solution of diazomethane (CH2N2) in ether was prepared and added (excess) in drops of the solutions of extracts, EF-1 and standard (1mg) CHCl3 or MeOH. The resulting solutions were allowed to stand for 12 hours and the ether removed by passing a stream of N2 [Leonard, Lygo, Procter, 1995].
