**Acknowledgements**

**Deletion and Insertion Mutations of Titin**

DCM 91043 del a^ N30348fs FNIII121 A-band Unknown [6] DCM 93376-93377 del ag^ R31126fs Ig139 A-band Unknown [6] DCM 97824-97831 del agtgacca\* A32606fs Ig147 M-band Unknown [6] DCM 98964 del a\* K32987fs Ig150 M-band Unknown [6] **Insertion Mutations** DCM 38621 ins a^ A12873fs Ig85 I-band Unknown [6] DCM 28kb duplication of ex 72-124^ Ig50-PEVK I-band Unknown [6] DCM 53145 ins g^ E17715fs FNIII28 A-band Unknown [6] DCM 58880 insert a\* S19628fs FNIII42 A-band Truncation of titin [10] DCM 62986-62987 ins at ex. 326\* K20995fs Ig115 A-band Truncation of titin [20] DCM 72178 ins t^ Q24059fs FNIII75 A-band Unknown [6] DCM 78372 ins a\* G26124fs FNIII97 A-band Unknown [10] DCM 90493 ins cct^ T30165fs FNIII120 A-band Unknown [6] DCM 91537 ins a^ T30513fs FNIII122 A-band Unknown [6] **Indel (Insertion/Deletion) Mutations**

**Protein**

276 del g ins gt^ T92fs Ig1 Z-disc Unknown [5] 594-598 del actt ins a^ E198del Ig2 Z-disc Unknown [5] 1458 del c ins ct^ A486fs Z-repeat 2 Z-disc Unknown [5] 6131 del c ins ct^ E2044fs 3' to Ig9 I-band Unknown [5] 28509-28513 del ctct ins c^ E9503del Ig77 I-band Unknown [5] 30939 del gt ins g^ T10313fs PEVK I-band Unknown [5] 31566 del a ins ag^ A10522fs PEVK I-band Unknown [5] 31605 del tc ins t^ K10535fs PEVK I-band Unknown [5]

35760 del a ins ag^ T11920fs PEVK I-band Unknown [5] 36267-36271 del cagg ins c^ P12089del Ig80 I-band Unknown [5] 39252-39254 del ttc ins t^ E13084fs Ig86 I-band Unknown [5] 65988 del gc ins g^ G21996fs Ig115 A-band Unknown [5]

77025 del a ins ag^ L25675fs Ig125 A-band Unknown [5] 86694-86697 del ttaa ins t^ K28898del Ig133 A-band Unknown [5] 97995-97998 del actt ins t^ K32665del Ig144 M-band Unknown [5] 101133 del ga ins g^ S33711fs Ig148 M-band Unknown [5]

DCM 67057-67063 del gcatatg ins ta^ A22353fs Ig116 A-band Unknown [6] DCM 72723-72739 del a ins aga^ S24241fs Ig121 A-band Unknown [6]

**Table 3.** Listing of deletion and insertion mutations found in TTN that have been causally linked to the development

NM\_001256850.1^ for titin DCM: dilated cardiomyopathy, HCM: hypertrophic cardiomyopathy, del: deletion, ins:

of cardiomyopathies. Sequences correspond to the following accession numbers: NM\_133378.4\* and

I11443-E11449del PEVK I-band Unknown [5]

**Sarcomeric**

**Region Effect Reference**

**Disease Mutation Amino Acid Region on**

76 Cardiomyopathies

34329-34347 del tttcctcttcaggagcaa ins t^

insertion, fs: frameshift

Our research has been supported by grants to A.K.K. through the National Institutes of Health (R21 HL106197) and the American Heart Association (GRNT 3780035) and to M.A.A from the National Institutes of Health (F32 AR058079).
