**5. Conclusions**

The HA-particles are essential in this protocol both for their adsorption properties for proteins of interest and their adjuvant properties. They constitute a tool which allows to purify tumor associated antigens in a fast and reproducible way.

The overall survival of the four dogs was much better than expected. These preliminary results nonetheless show that the technique is feasible in private veterinary medicine. They are consistant with other results obtained with canine osteosarcomas or with a series of stage Vb lymphomas treated by chemotherapy and this protocol.

The association of the HA-particles to tumor associated antigens and HSPs did not trigger any adverse effects, confirming the safe results obtained for other applications in human medicine.

This technique could also be combined with conventional chemo- or radiotherapy to increase the animal's overall survival.
