**3. Platelet immunofluorescence**

The platelet count in the PRP was adjusted to 300.000 μL-1 by dilution with homologous PPP. After 1 hour contact of PRP with the different specimens at 37 °C, samples were washed with PBS, followed by fixation with 3% (w/v) paraformaldehyde and incubated with 1% (w/v) BSA in PBS. Labelling of the platelets was performed with a mouse monoclonal antibody CD62P (anti-P-Selectin, Santa Cruz Biotechnology, USA) at dilution 1:100, followed by 1:200 diluted monoclonal goat anti-mouse IgG antibody, FITC conjugated (Sigma-Aldrich, USA).
