**Author details**

Jean-Michel Bourget1,2,3, Maxime Guillemette4 , Teodor Veres5 , François A. Auger1,2 and Lucie Germain1,2

1 Laval University LOEX center, Tissue Engineering And Regenerative Medecine : LOEX – FRQS Research Center of the "Centre Hospitalier Affilié Universitaire de Québec", Canada

2 Department of Surgery, Faculty of Medicine, Laval University, Quebec, Canada

3 National Research Council Canada, Boucherville, PQ, Canada

4 Physics Department, Faculty of Science and Engineering, Laval University, and Medical Physics Unit, "Centre Hospitalier Universitaire de Québec", Québec, QC, Canada

5 Life Sciences Division, National Research Council Canada, Biomedical Engineering, McGill University, National Research Council Canada, Boucherville, PQ, Canada

#### **References**


[15] Lynch HA, Johannessen W, Wu JP, Jawa A, Elliott DM. Effect of fiber orientation and strain rate on the nonlinear uniaxial tensile material properties of tendon. J Biomech Eng. 2003;125:726-31.

**References**

2007;19:43-50.

380 Advances in Biomaterials Science and Biomedical Applications

2005;62:1081-99.

2005;289:H2048-58.

2001;49:353-63.

2009;15:378-85.

2011;52:1243-51.

[1] Alberts B. Molecular biology of the cell. 5th ed. New York: Garland Science; 2008.

[2] Delon I, Brown NH. Integrins and the actin cytoskeleton. Curr Opin Cell Biol.

[3] Arnaout MA, Mahalingam B, Xiong JP. Integrin structure, allostery, and bidirectional

[4] Wiesner S, Legate KR, Fassler R. Integrin-actin interactions. Cell Mol Life Sci.

[6] Koubassova NA, Tsaturyan AK. Molecular mechanism of actin-myosin motor in

[7] Holzapfel GA, Sommer G, Gasser CT, Regitnig P. Determination of layer-specific me‐ chanical properties of human coronary arteries with nonatherosclerotic intimal thick‐ ening and related constitutive modeling. Am J Physiol Heart Circ Physiol.

[8] Wolinsky H, Glagov S. Structural Basis for the Static Mechanical Properties of the

[9] Robert L, Legeais JM, Robert AM, Renard G. Corneal collagens. Pathol Biol (Paris).

[10] Pinsky PM, van der Heide D, Chernyak D. Computational modeling of mechanical anisotropy in the cornea and sclera. J Cataract Refract Surg. 2005;31:136-45.

[11] Kamma-Lorger CS, Hayes S, Boote C, Burghammer M, Boulton ME, Meek KM. Ef‐ fects on collagen orientation in the cornea after trephine injury. Mol Vis.

[12] Boote C, Elsheikh A, Kassem W, Kamma-Lorger CS, Hocking PM, White N, et al. The influence of lamellar orientation on corneal material behavior: biomechanical and structural changes in an avian corneal disorder. Invest Ophthalmol Vis Sci.

[13] Boote C, Kamma-Lorger CS, Hayes S, Harris J, Burghammer M, Hiller J, et al. Quan‐ tification of collagen organization in the peripheral human cornea at micron-scale

[14] Lewis G, Shaw KM. Modeling the tensile behavior of human Achilles tendon. Bi‐

signaling. Annu Rev Cell Dev Biol. 2005;21:381-410.

muscle. Biochemistry (Mosc). 2011;76:1484-506.

Aortic Media. Circ Res. 1964;14:400-13.

resolution. Biophys J. 2011;101:33-42.

omed Mater Eng. 1997;7:231-44.

[5] Langer R, Vacanti JP. Tissue engineering. Science. 1993;260:920-6.


[44] Grinnell F, Lamke CR. Reorganization of hydrated collagen lattices by human skin fibroblasts. J Cell Sci. 1984;66:51-63.

[29] Parenteau-Bareil R, Gauvin R, Berthod F. Collagen-Based Biomaterials for Tissue En‐

[30] Yarlagadda PK, Chandrasekharan M, Shyan JY. Recent advances and current devel‐

[31] Shoulders MD, Raines RT. Collagen structure and stability. Annu Rev Biochem.

[32] Cliche S, Amiot J, Avezard C, Gariepy C. Extraction and characterization of collagen with or without telopeptides from chicken skin. Poultry Science. 2003;82:503-9.

[33] Parenteau-Bareil R, Gauvin R, Cliche S, Gariepy C, Germain L, Berthod F. Compara‐ tive study of bovine, porcine and avian collagens for the production of a tissue engi‐

[34] L'Heureux N, Germain L, Labbe R, Auger FA. In vitro construction of a human blood vessel from cultured vascular cells: a morphologic study. J Vasc Surg.

[35] Tranquillo RT, Durrani MA, Moon AG. Tissue engineering science: consequences of

[36] Lopez Valle CA, Auger FA, Rompre P, Bouvard V, Germain L. Peripheral anchorage

[37] Barocas VH, Tranquillo RT. An anisotropic biphasic theory of tissue-equivalent me‐ chanics: the interplay among cell traction, fibrillar network deformation, fibril align‐

[38] Eastwood M, Porter R, Khan U, McGrouther G, Brown R. Quantitative analysis of collagen gel contractile forces generated by dermal fibroblasts and the relationship to

[39] Thomopoulos S, Fomovsky GM, Chandran PL, Holmes JW. Collagen fiber alignment does not explain mechanical anisotropy in fibroblast populated collagen gels. J Bio‐

[40] Chandran PL, Barocas VH. Affine versus non-affine fibril kinematics in collagen net‐ works: theoretical studies of network behavior. J Biomech Eng. 2006;128:259-70.

[41] Chandran PL, Barocas VH. Deterministic material-based averaging theory model of

[42] Costa KD, Lee EJ, Holmes JW. Creating alignment and anisotropy in engineered heart tissue: role of boundary conditions in a model three-dimensional culture sys‐

[43] Klebe RJ, Caldwell H, Milam S. Cells transmit spatial information by orienting colla‐

opments in tissue scaffolding. Biomed Mater Eng. 2005;15:159-77.

gineering Applications. Materials. 2010;3:1863-87.

neered dermis. Acta Biomater. 2011;7:3757-65.

cell traction force. Cytotechnology. 1992;10:225-50.

cell morphology. J Cell Physiol. 1996;166:33-42.

mech Eng. 2007;129:642-50.

tem. Tissue Eng. 2003;9:567-77.

gen fibers. Matrix. 1989;9:451-8.

of dermal equivalents. Br J Dermatol. 1992;127:365-71.

ment, and cell contact guidance. J Biomech Eng. 1997;119:137-45.

collagen gel micromechanics. J Biomech Eng. 2007;129:137-47.

2009;78:929-58.

382 Advances in Biomaterials Science and Biomedical Applications

1993;17:499-509.


calcium: preferential response of poorly differentiated cells. J Cell Physiol. 2012;227:2660-7.


[71] Torbet J, Freyssinet JM, Hudry-Clergeon G. Oriented fibrin gels formed by polymeri‐ zation in strong magnetic fields. Nature. 1981;289:91-3.

calcium: preferential response of poorly differentiated cells. J Cell Physiol.

[58] Sun LY, Hsieh DK, Yu TC, Chiu HT, Lu SF, Luo GH, et al. Effect of pulsed electro‐ magnetic field on the proliferation and differentiation potential of human bone mar‐

[59] Tranquillo RT, Girton TS, Bromberek BA, Triebes TG, Mooradian DL. Magnetically orientated tissue-equivalent tubes: application to a circumferentially orientated me‐

[60] Tsai MT, Chang WH, Chang K, Hou RJ, Wu TW. Pulsed electromagnetic fields affect osteoblast proliferation and differentiation in bone tissue engineering. Bioelectro‐

[61] Kotani H, Kawaguchi H, Shimoaka T, Iwasaka M, Ueno S, Ozawa H, et al. Strong static magnetic field stimulates bone formation to a definite orientation in vitro and

[62] Builles N, Janin-Manificat H, Malbouyres M, Justin V, Rovere MR, Pellegrini G, et al. Use of magnetically oriented orthogonal collagen scaffolds for hemi-corneal recon‐

[63] Morin KT, Tranquillo RT. Guided sprouting from endothelial spheroids in fibrin gels aligned by magnetic fields and cell-induced gel compaction. Biomaterials.

[64] Worcester DL. Structural origins of diamagnetic anisotropy in proteins. Proc Natl

[65] Méthot S, Moulin V, Rancourt D, Bourdages MG, D., Plante M, Auger FA, et al. Mor‐ phological changes of human skin cells exposed to a DC electric field in vitro using a

[66] Messerli MA, Graham DM. Extracellular electrical fields direct wound healing and

[67] Zhao M, McCaig CD, Agius-Fernandez A, Forrester JV, Araki-Sasaki K. Human cor‐ neal epithelial cells reorient and migrate cathodally in a small applied electric field.

[68] Sunkari VG, Aranovitch B, Portwood N, Nikoshkov A. Effects of a low-intensity elec‐ tromagnetic field on fibroblast migration and proliferation. Electromagn Biol Med.

[69] Higashi T, Yamagishi A, Takeuchi T, Kawaguchi N, Sagawa S, Onishi S, et al. Orien‐ tation of erythrocytes in a strong static magnetic field. Blood. 1993;82:1328-34.

[70] Kotani H, Iwasaka M, Ueno S, Curtis A. Magnetic orientation of collagen and bone

row mesenchymal stem cells. Bioelectromagnetics. 2009;30:251-60.

dia-equivalent. Biomaterials. 1996;17:349-57.

in vivo. J Bone Miner Res. 2002;17:1814-21.

struction and regeneration. Biomaterials. 2010;31:8313-22.

new exposure system. Can J Chem Eng. 2001;79:668-77.

mixture. Journal of Applied Physics. 2000;87:6191-3.

magnetics. 2007;28:519-28.

2011;32:6111-8.

Acad Sci U S A. 1978;75:5475-7.

regeneration. Biol Bull. 2011;221:79-92.

Curr Eye Res. 1997;16:973-84.

2011;30:80-5.

2012;227:2660-7.

384 Advances in Biomaterials Science and Biomedical Applications


[101] Boland ED, Bowlin GL, Simpson DG, Wnek GE. Electrospinning of tissue engineer‐ ing scaffolds. Abstracts of Papers of the American Chemical Society. 2001;222:U344- U.

[86] Nisbet DR, Forsythe JS, Shen W, Finkelstein DI, Horne MK. Review paper: a review of the cellular response on electrospun nanofibers for tissue engineering. J Biomater

[87] Sill TJ, von Recum HA. Electrospinning: applications in drug delivery and tissue en‐

[88] Murugan R, Ramakrishna S. Design strategies of tissue engineering scaffolds with

[89] Teo WE, Ramakrishna S. A review on electrospinning design and nanofibre assem‐

[90] Liao S, Li B, Ma Z, Wei H, Chan C, Ramakrishna S. Biomimetic electrospun nanofib‐

[91] Formhals A. Process and apparatus for preparing artificial threads. In: Patent U, edi‐

[92] Li WJ, Mauck RL, Cooper JA, Yuan X, Tuan RS. Engineering controllable anisotropy in electrospun biodegradable nanofibrous scaffolds for musculoskeletal tissue engi‐

[93] Xu CY, Inai R, Kotaki M, Ramakrishna S. Aligned biodegradable nanofibrous struc‐ ture: a potential scaffold for blood vessel engineering. Biomaterials. 2004;25:877-86.

[94] Pham QP, Sharma U, Mikos AG. Electrospinning of polymeric nanofibers for tissue

[95] Subbiah T, Bhat GS, Tock RW, Pararneswaran S, Ramkumar SS. Electrospinning of

[96] Li WJ, Laurencin CT, Caterson EJ, Tuan RS, Ko FK. Electrospun nanofibrous struc‐ ture: a novel scaffold for tissue engineering. J Biomed Mater Res. 2002;60:613-21. [97] Li WJ, Danielson KG, Alexander PG, Tuan RS. Biological response of chondrocytes cultured in three-dimensional nanofibrous poly(epsilon-caprolactone) scaffolds. J Bi‐

[98] Geng X, Kwon OH, Jang J. Electrospinning of chitosan dissolved in concentrated ace‐

[99] Venugopal J, Ramakrishna S. Biocompatible nanofiber matrices for the engineering

[100] Peretti GM, Randolph MA, Zaporojan V, Bonassar LJ, Xu JW, Fellers JC, et al. A bio‐ mechanical analysis of an engineered cell-scaffold implant for cartilage repair. An‐

of a dermal substitute for skin regeneration. Tissue Eng. 2005;11:847-54.

engineering applications: a review. Tissue Eng. 2006;12:1197-211.

nanofibers. Journal of Applied Polymer Science. 2005;96:557-69.

Appl. 2009;24:7-29.

386 Advances in Biomaterials Science and Biomedical Applications

tor. USA1934.

gineering. Biomaterials. 2008;29:1989-2006.

blies. Nanotechnology. 2006;17:R89-R106.

neering. J Biomech. 2007;40:1686-93.

omed Mater Res A. 2003;67:1105-14.

nals of Plastic Surgery. 2001;46:533-7.

tic acid solution. Biomaterials. 2005;26:5427-32.

controlled fiber orientation. Tissue Eng. 2007;13:1845-66.

ers for tissue regeneration. Biomed Mater. 2006;1:R45-53.


[128] Seliktar D, Nerem RM, Galis ZS. The role of matrix metalloproteinase-2 in the remod‐ eling of cell-seeded vascular constructs subjected to cyclic strain. Ann Biomed Eng. 2001;29:923-34.

[114] Teixeira AI, Abrams GA, Bertics PJ, Murphy CJ, Nealey PF. Epithelial contact guid‐ ance on well-defined micro- and nanostructured substrates. J Cell Sci.

[115] Teixeira AI, Nealey PF, Murphy CJ. Responses of human keratocytes to micro- and

[116] Isenberg BC, Tsuda Y, Williams C, Shimizu T, Yamato M, Okano T, et al. A thermor‐ esponsive, microtextured substrate for cell sheet engineering with defined structural

[117] Sarkar S, Dadhania M, Rourke P, Desai TA, Wong JY. Vascular tissue engineering: microtextured scaffold templates to control organization of vascular smooth muscle

[118] Okano T, Yamada N, Okuhara M, Sakai H, Sakurai Y. Mechanism of cell detachment from temperature-modulated, hydrophilic-hydrophobic polymer surfaces. Biomate‐

[119] Isenberg BC, Backman DE, Kinahan ME, Jesudason R, Suki B, Stone PJ, et al. Micro‐ patterned cell sheets with defined cell and extracellular matrix orientation exhibit

[120] Guillemette MD, Roy E, Auger FA, Veres T. Rapid isothermal substrate microfabrica‐ tion of a biocompatible thermoplastic elastomer for cellular contact guidance. Acta

[121] L'Heureux N, Paquet S, Labbe R, Germain L, Auger FA. A completely biological tis‐

[122] Auger FA, Rémy-Zolghadri M, Grenier G, Germain L. The Self-Assembly Approach for Organ Reconstruction by Tissue Engineering. e-biomed: The Journal of Regenera‐

[123] Guillemette MD, Park H, Hsiao JC, Jain SR, Larson BL, Langer R, et al. Combined technologies for microfabricating elastomeric cardiac tissue engineering scaffolds.

[125] Kanda K, Matsuda T. Mechanical stress-induced orientation and ultrastructural change of smooth muscle cells cultured in three-dimensional collagen lattices. Cell

[126] Niklason LE, Gao J, Abbott WM, Hirschi KK, Houser S, Marini R, et al. Functional

[127] Seliktar D, Black RA, Vito RP, Nerem RM. Dynamic mechanical conditioning of col‐ lagen-gel blood vessel constructs induces remodeling in vitro. Ann Biomed Eng.

[124] Risau W, Flamme I. Vasculogenesis. Annu Rev Cell Dev Biol. 1995;11:73-91.

nanostructured substrates. J Biomed Mater Res A. 2004;71:369-76.

cells and extracellular matrix. Acta Biomater. 2005;1:93-100.

anisotropic mechanical properties. J Biomech. 2012;45:756-61.

sue-engineered human blood vessel. Faseb J. 1998;12:47-56.

organization. Biomaterials. 2008;29:2565-72.

2003;116:1881-92.

388 Advances in Biomaterials Science and Biomedical Applications

rials. 1995;16:297-303.

Biomater. 2011;7:2492-8.

tive Medicine. 2000;1:75-86.

Transplant. 1994;3:481-92.

2000;28:351-62.

Macromol Biosci. 2010;10:1330-7.

arteries grown in vitro. Science. 1999;284:489-93.

