**5. Conclusion**

confluency and stimulated to synthesize ECM by culture medium supplemented ascorbic acid. Matrices are denuded of cells and cellular remnants are removed by using Triton X-100,

Then, ECM coated culture surfaces are tested by staining with PI to access DNA remnant (see Figure 9), with H&E and PAS in order to characterize component of ECM. The results suggest that fibroblast-derived three-dimensional matrix was determined to be free of cellular constituents and still remain attached to the culture surface. The conducted matrices were

(for example, induction of cell attachment, proliferation…) was reported to be well-preserved

In order to evaluate the quality of the ECM, the test of cell rapid attachment ability is per‐ formed. Cell proliferation on ECM is also assessed. Results showed that the cultured cells attached and proliferated on ECM coated surfaces faster than on ECM non-coated surfaces.

C. Under these conditions, biological activity

NH4OH and DNase.

268 Regenerative Medicine and Tissue Engineering

for up to 6 months.

washed and covered with PBS; and stored at 40

a b

a b

**Figure 9.** ECM stained PI before (a) and after (b) using DNase

**Figure 8.** Fibroblasts are in cultured surfaces (a) and are stimulated to synthesize ECM (b)

Inmodernsociety,manydiseaseshavebeenincreasinginhumanbecauseofpollution, accident, lifestyle... The mutilation in human body leads to expand the needs of replacing tissues/ organs. However, the available source of tissues/organs is limited. Creating artificial tissues/ organsforreplacingdamaged,dysfunctionaltissues/organsbecomesabigdisciplineonmaterial science. Naturally derived biomaterials have been studied and applied in clinical applications as artificial tissues/organs because they are capable of supporting cell proliferation, biodegrad‐ ability and remodeling tissues. Although the current results have not completely satisfy the clinical demand, the potential applications of naturally derived biomaterials are still highly considered, therefore, research on this field have now being taken place all over the world.
