**Acknowledgements**

unwanted cell-cueing signals, mechanical properties, etc. thus making 3D culturing, along with regenerative medicine multidisciplinary fields. However, as science advances, more and more questions emerge and await answers in order to confirm the long term safety of the

**Figure 2.** General strategy for design of a "perfect" 3D system. The specific set of ECM characteristics, typical for se‐ lected normal tissue can be determined. Based on these studies, a scaffold with matching parameters and suitable cells can be identified and combined in vitro for development of particular, tailored made 3D culture, meeting the

During the resolution of the moral issues concerning the use of human embryonic stem cells or donor organs the remaining fields continue to advance rapidly. It appears that isolation of autologus cells, their expansion in vitro and probably stimulation to produce extracellular matrix with the required dimensionality, biochemical and mechanical properties would represent the optimal tissue engineered scaffold for tissue and organ reconstruction. Further advance in the field of regenerative medicine and tissue engineering would possibly see the development of 4D model systems, incorporating time as the forth dimension. Such models would represent not just single stages of organ development or disease, but the chronology of

applied methods for human therapy.

236 Regenerative Medicine and Tissue Engineering

needs of a specific research or specific therapy.

This publication is supported with funding form Grant № ДДВУ02/9 by the National Science Fund
