**7. Hematoxylin and eosin staining of scalp biopsy**

The histological findings in many forms of hair loss may be similar, and an accurate diagnosis of hair loss depends on distinguishing abnormal from normal follicular architecture. It is important to identify the normal hair follicle structure, the number, size and distribution of hair follicles within a biopsy specimen. Hematoxylin and eosin staining of the scalp biopsies is the usual satin in most of the cases of hair loss, but in some of the alopecias (such as DLE), immunofluorescence staining may be needed to add in diagnosis. In addition, the pathologist may use additional special stains to narrow a differential diagnosis or confirm an initial impression and one of these is immunohistochemistry which is dependent on the localization of antigens in tissue sections by the use of labeled antibody as specific reagents through antigen-antibody interactions that are visualized by a marker such as peroxidase.
