**16. Bowman-Birk Inhibitor (BBI)**

KQGQHQQEEEEE), corresponding to residues 202 to 213 and residues 214 to 225 of the gly‐ cinin G4 subunit, elevated the number of interleukin (IL)-12(+)CD11b(+) cells. Peptide 202-213 increased the number of CD49b(+), IL-2(+)CD4(+), and interferon-gamma(+)CD4(+) cells, and stimulated the cytotoxic activity of splenocytes toward K562 human erythroleuke‐ mia cells. The data suggest that the glutamine-rich region of the soybean glycinin G4 subu‐

A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen

Soy protein mitigates lipotoxicity in the liver and pancreas in various rodent models of obe‐ sity. Dietary soy protein can suppress cardiac cholesterol and triglyceride concentrations and reduce cardiacceramide concentration by downregulating the expression of serine pal‐ mitoyl transferase, a key enzyme in sphingolipid biosynthesis in the heart of obese rodents, and by reducing lipid accumulation. Thus, soy protein consumption may be a useful dietary

Cha and Park [16] produced a soy protein-derived ACE inhibitory hydrolysate and charac‐ terized its activity, physicochemical, and biochemical properties. The competitive ACE in‐

Two non-competitive ACE inhibitors, Ile-Phe-Leu (IC50, 44.8 M) and Trp-Leu (IC50, 29.9 M), were isolated from the tofuyo (fermented soybean food) extract. The first sequence is con‐ tained in the alpha- and beta-subunits of beta-conglycinin, while the second sequence exists in the B-, B1A- and BX-subunits of soybean glycinin. The inhibitory activity of Trp-Leu was unaffected by the gastrointestinal proteases pepsin, chymotrypsin and trypsin. About 30%

**15. Angiotensin I-converting enzyme inhibitory peptide derived from**

The pentapeptide Val-Leu-Ile-Val-Pro derived from glycinin has an IC50 of 1.69 +/- 0.17 M and a Ki of 4.5 +/- 0.25 x 10-6 M for ACE inhibition. This peptide was resistant to digestion by

hibitory activity remained stable after incubation with gastric enzymes for 2 hours

nit enhances the cellular immune system in murine splenocyte cultures [14].

therapeutic strategy for l prevention of lipotoxic cardiomyopathy [15].

**13. Soy protein-derived angiotensin I-converting enzyme**

**14. Angiotensin I-converting enzyme inhibitory peptides**

of the activity remained after successive digestion by these proteases [17].

**12. Soy protein**

Relationships

244

**glycinin**

gastrointestinal proteases [18].

**(ACE)inhibitory hydrolysate**

The low cancer incidence associated with soybean consumption has been related to the pres‐ ence of Bowman-Birk inhibitor (BBI) and lectin [19]. Two different strategies were used for the extraction of BBI and lectin. The first method entailed extraction of soybean proteins us‐ ing Tris-HCl buffer followed by isolation of BBI and lectin by isoelectric precipitation of oth‐ er soybean proteins. The second method involved direct extraction of BBI and lectin using an acetate buffer. The effect of previous delipidation on the extraction of BBI and lectin was examined. The possibility of using a high-intensity focalized ultrasonic probe for expediting the extraction was investigated and the extraction time and ultrasound amplitude were opti‐ mized. After evaluation of its analytical characteristics (linearity, precision, and recovery), the method was used for quantitating BBI and lectin in different varieties of soybean [19].
