**18. Kunitz trypsininhibitor**

Duranti et al described a method for the extraction and purification of Kunitz trypsin inhibi‐ tor from soybean seeds. The first step involved heat treatment of whole soybean seeds in water at 60 o C for 90 min. It was found that 8.4% of total trypsin inhibitory activity of the seeds was secreted during heat treatment. The aqueous medium was applied on an affinity chromatography column with immobilized trypsin. The method is readily scalable to pilot plant or industrial preparations [21].
