**9. Conclusion**

The initial hypothesis formulated that the genetic modification itself is stressing the soy‐ bean, is apparently right, once the plant is searching a new equilibrium as living organism. The results presented in this chapter demonstrate that not only is the proteomic map changed with some proteins increasing and others decreasing, but also chromatographic separations are altered when transgenic and non-transgenic soybeans are compared. Exam‐ ples are activities of some enzymes (as CAT, SOD, GPx, among others) involved in neutrali‐ zation of ROS, as well as the possible capacity in taking metals from the soil (mainly for Fe and Cu). Because of these modifications that occur when both transgenic and non-transgenic organisms are compared, the theme of genetic modification could be even better explained with some alternative strategies, such as quantitative proteomics, image analysis, tracer ex‐ periments with stable isotopes, and other possibilities.

Finally, in our point of view, one of the key points for the success of studies involving trans‐ genic organisms is not only to involve good technology, but also a transdisciplinary view, involving different areas of expertise. With this strategy, it will be easier to understand this area of investigation, making possible the demystification of the genetic modification that have occurred, and allowing answers for some questions that still remain unknown.

### **10. Nomenclature and acronyms**

2-D-HPLC Two-Dimensional High Performance Liquid Chromatography

2-D PAGE Two-Dimensional Gel Electrophoresis

2-D DIGE Two-Dimensional Difference Gel Electrophoresis

ICP-MS Inductively Coupled Plasma Mass Spectrometry

HR-SF-ICP-MS High Resolution Sector Field Inductively Coupled Plasma Mass Spectrometry

LA-ICP-MS Laser Ablation Inductively Coupled Plasma Mass Spectrometry

MALDI-QTOF-MS Matrix-Assisted Laser Desorption Ionisation Quadrupole-Time-of-Flight Mass Spectrometry

ESI-LC-MS-MS ElectroSpray Ionization Liquid Chromatography Mass Spectrometry-Mass Spectrometry

2-D-HPLC-ICP-MS Two-Dimensional High Performance Liquid Chromatography Induc‐ tively Coupled Plasma Mass Spectrometry

SDS-PAGE Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis

ESI-QTOF MS-MS ElectroSpray Ionization-Time-of-Flight Mass Spectrometry-Mass Spectrome‐ try

SEC-ICP-MS Size Exclusion Chromatography-Inductively Coupled Plasma Mass Spectrometry
