**5. Soybean 7S globulin**

**2. Storage proteins**

Relationships

240

mately 44 and 34, respectively [1].

**3. β-conglycinin**

**4. Glycinin**

relative humidity at 20 o

tion of previously inconspicuous proteins in soybean seed [2].

changes the action of the drug in cholesterol homeostasis [3].

C, (iii) in the cold (4 o

ditions (i) in 84% relative humidity at 30 o

A combined proteomic approach was employed for the separation, identification, and comparison of two major storage proteins, beta-conglycinin and glycinin, from cultivat‐ ed (*Glycine max*) and wild (*Glycine soja*) soybean seeds. Two-dimensional polyacrylamide gel electrophoresis with three different immobilized pH gradient strips effectively re‐ solved many storage proteins. The pH range 3.0 - 10.0 was good for separating most of the beta-conglycinin subunits while pH ranges 4.0 - 7.0 and 6.0 - 11.0 were satisfactory for separating acidic and basic glycinin polypeptides, respectively. Although the distri‐ bution pattern of the protein spots was in general alike in both genotypes by employ‐ ing pH 3.0 - 10.0, variations in number and intensity of protein spots were better resolved when a combination of pH 4.0 - 7.0 and pH 6.0 - 11.0 was utilized. The total number of storage protein spots detected in wild and cultivated genotypes was approxi‐

A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen

Krishnan et al have developed a fast and simple fractionation technique using 10 mM Ca2+ to precipitate soybean seed storage globulins, glycinin and beta-conglycinin. This method eliminates over 80% of the highly abundant seed proteins from the extract, facilitating detec‐

Ferreira et al conducted a study to investigate the effect of daily oral administration of soy‐ bean β-conglycinin (7S protein) (300 mg/kg/day) alone and in conjunction with the hypolipi‐ demic drugs fenofibrate (30 mg/kg/day) and rosuvastatin (10 mg/kg/day) in hypercholesterolemic rats for 28 days. The data reveal that conglycinin exhibits effects simi‐ lar to those of fenofibrate and rosuvastatin in the control of plasma cholesterol, HDL-C and triglycerides, and suggests that the association of soybean β-conglycinin with rosuvastatin

Glycinin was isolated and characterized from soybeans stored under different ambient con‐

for 18 months.. Glycinin purified from soybean exposed to the adverse conditions possessed a significant amount of sugar and demonstrated a decrease in hydrophobic interactions after 3 months; the total free sulfhydryl content in glycinin dropped, but the intramolecular disul‐ fide bonds increased; the alpha-helix content of secondary structure underwent a slight rise, but the beta-sheet content fell. The structure of glycinin purified from and subjected to the other three aforementioned conditions remained unaltered after 18 months of storage when

C (adverse conditions) for 9 months and (ii) in 57%

C), and (iv) in an uncontrolled ambient garage

In rats fed a hypercholesterolemic diet soybean 7S globulin reduced plasma cholesterol and triglycerides and upregulated liver beta- very-low-density lipoprotein (VLDL) receptors [7].
