**Author details**

bined with VP64 activator (derived from the herpex simplex virus activation domain). VP64 binds with RNA polymerase and causes transcriptional activation of the gene of interest [99]. For making transgenic plants, the TALEs technique can be combined with the *Agrobac‐ terium*-mediated transformation method and it is assumed that gene targeting knock-out

A Comprehensive Survey of International Soybean Research - Genetics, Physiology, Agronomy and Nitrogen

will be applied in soybean research.

Relationships

498

**Figure 2.** Summary of Transcription activator-like effectors (TALEs) nuclease.

Acetosyringone: 4'-Hydroxy-3',5'-dimethoxyacetophenone

**6. Abbreviation**

*bar*: bialaphos resistance

BT: *Bacillus thuringiensis*

DSBs: double-strand breaks

BAP: 6-Benzylaminopurine

BAC: bacterial artificial chromosome

2,4-D: 2,4-Dichlorophenoxyacetic acid

HPT II: Hygromycin phosphotransferase

BIBAC: binary bacterial artificial chromosome

Hyeyoung Lee, So-Yon Park and Zhanyuan J. Zhang\*

\*Address all correspondence to: zhangzh@missouri.edu

Plant Transformation Core Facility, University of Missouri, Columbia, USA
