**8. Conclusion**

The sex sorting process by flow cytometry is aggressive to the spermatozoa, compromising the viability of these cells. Thus, some adjustments in the mode of use the sexed semen in AI have to be done to improve the tangibility in reproductive programs at livestock level. Ad‐ justments in the AI moment in relation to the estrus beginning improve the conception rate. The results of the studies presented, indicates that beef and dairy heifers inseminated with sex-sorted sperm after estrus detection have satisfactory conception rate, and the AI has to be done between 16 to 24 h after the estrus commencement (6 to 14 h before ovulation). Al‐ so, when using synchronization of ovulation protocols for TAI in beef and dairy cows, ac‐ ceptable conception rate is obtained when TAI is done at 60 h after P4 device removal (10 h before ovulation). Additionally, there is a huge individual variation of fertility among se‐ men of sires submitted to the sexing process, and has to be considered at the moment of bull choosing in AI programs.
