**Acknowledgement**

The financial support from National Natural Science Foundation of China (Project number 21177156 and 20907073) for this work is hereby gratefully acknowledged.

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**Chapter 12** 

© 2013 Kaminski et al., licensee InTech. This is an open access chapter distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

© 2013 Kaminski et al., licensee InTech. This is a paper distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

**Cellulose Functionalysed with** 

Additional information is available at the end of the chapter

Justyna Fraczyk, Beata Kolesinska, Inga Relich and Zbigniew J. Kaminski

Advantages offered by immobilization of any component of the reacting system are rewarding all additional efforts and the cost of the support. The majority of the methods reported have been based on the principles of solid phase organic synthesis (SPOS) in which the substrate is attached to the polymer support and excesses of reactants and reagents used to drive each synthetic step to completion. Then simple filtration affords a polymer bound product. While this approach is undoubtedly effective, there are a number of drawbacks which include the requirement for additional chemical steps to attach starting material, to develop synthetic methodology for the solid phase and to cleave products. More recently, solution phase methods, which circumvent these difficulties, have been introduced as alternatives to SPOS. These allow the use of excess of reagents followed by sequestrating either the product or excess reagents and byproducts from the reaction mixture using an insoluble functionalized polymer. Isolation and purification can then be achieved by simple

Usually polystyrene and PEG based resins are commonly used as matrixes in SPOS, but nowadays there is observed also increased application of various beaded cellulose supports [1]. These show different solvent swelling profiles relative to those exhibited by the standard organic polymers and, being biomolecules, are biodegradable. Cellulose framework is attracting growing attention due to favorable biophysical properties, biocompatibility, low immunogenicity, relatively high resistance to temperature, inertness under broad range of reaction conditions and solvents and many other unique properties. Moreover, native cellulose microfribrils are abundant within slightly diversified properties dependent on the origin within relatively low cost. These properties make cellulose very useful for biochemical and biological investigations of interactions in aqueous as well as organic

**Grafted Oligopeptides** 

http://dx.doi.org/10.5772/52302

filtration and evaporation.

media.

**1. Introduction** 

