**Acknowledgement**

The authors highly appreciate the financial support of Key project of National Basic Science and Development (G1999064707), the Project of 863(N0.2002AA326060)and Tianjin-Nankai University Co-Construction Foundation.

#### **6. References**

210 Cellulose – Medical, Pharmaceutical and Electronic Applications

NOTE: a and b stand for the results before and after WBIA (From Li X H et al, Artif. Cell Blood Sub (2010), 38:186-191)

neuromuscular junction and antibody titer.

prolonging their survival rates.

**Author details** 

Shenqi Wang

Yaoting Yu

*P.R. China* 

**Acknowledgement** 

**5. Conclusion and future perspectives** 

Nankai University Co-Construction Foundation.

day(200)

**Figure 11.** Neuromuscular junctions of the therapeutic rabbit group before and after WBIA on the 3rd

In conclusion, extracorporeal whole blood sorbent-adsorption is an effective and safe approach in treating the passive experimental autoimmune myasthenia gravis by improving clinical manifestation, neuromuscular transmission function, enhancing the quantity of

Intensive research and development of cellulosic bead type adsorbents by sorbent-perfusion in blood purification have paved a path for the treatment of patients with autoimmune diseases, hyperlipidemia and inflammatory disorders. Animal experiments and clinical trials have proved that it is safe, efficient and cost effective. Therefore, it is highly potential to be used clinically on patients for upgrading the quality of their living standard and

*College of Life Science and Technology & Advanced Biomaterials and Tissue Engineering Center,* 

*The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin,* 

The authors highly appreciate the financial support of Key project of National Basic Science and Development (G1999064707), the Project of 863(N0.2002AA326060)and Tianjin-

*Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan, P.R. China* 

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**Chapter 11** 

© 2013 Qiu et al., licensee InTech. This is an open access chapter distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

© 2013 Qiu et al., licensee InTech. This is a paper distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

**The Development and Application of Cellulose-**

In the 1980s, polysaccharide-based chiral stationary phases (CSPs) were identified as versatile and useful chiral sorbents for separation of enantiomers/stereoisomers in high performance liquid chromatography (HPLC). Chiral discrimination abilities of these CSPs can be derived from the highly organized structure of the left-handed 3/2 helical chain conformations [1]. Some chiral cavities with specific configuration can be formed on the CSPs, which provide the suitable site for a particular enantiomer and make it easier to interact with CSPs by hydrogen bonding and - interactions. This leads to enantioseparation of chiral compounds by different retention and elution on CSPs between their enantiomers [2]. Okamoto et al. reported that the introduction of various kinds of substituents on the hydroxyl group of polysaccharides can improve their stereoslectivity [3].

Cellulose is an important polysaccharide, it is also a highly crystalline polymer which occurs with various crystal structures. In the 1970s, Hesse and Hagel first synthesized microcrystalline cellulose triacetate (MCTA), and thought its chiral recognition ability might originate from secondary structures creating chiral cavities upon swelling, which can clamp stereoselectively compounds with aromatic residues [4]. In recent years, different cellulose derivatives have been synthesized, coated or covalently bonded on decorative silica gel, and broadly used as CSPs in enantiomeric separation of chiral compounds especially on pesticides and pharmaceuticals. These derivatives exhibit powerful chiral recognition ability towards a wide number of different racemic compounds. More and more commercial cellulose-based CSPs including cellulose acetate, benzoate and phenylcarbamates are being

**Based Stationary Phases in Stereoselective** 

**Separation of Chiral Pesticides** 

Additional information is available at the end of the chapter

developed and applied in enantioseparation [2,3].

Shuming Yang and Hualin Zhao

http://dx.doi.org/10.5772/56575

**1. Introduction** 

Jing Qiu, Shouhui Dai, Tingting Chai, Wenwen Yang,

