**5. Conclusion**

232 Cellulose – Medical, Pharmaceutical and Electronic Applications

colum

Lux Cellulose-1 Chiralcel OD-H

75 triadimenol Chiralcel OD-H hexane/IPA(100/3); 1.0

76 trichlorfon CDMPC ACN/water(70/30);

78 uniconazole CDMPC hexane/ n-butyl

79 vinclozolin CDMPC hexane/IPA(99/1);

respectively.

Chromatographic condition\*1

mL/min; UV 225nm

ACN/water(70/30), MET/water(90/10); 1.0 mL/min; UV 220nm

mL/min; UV 225nm

mL/min; UV 225nm

mL/min; UV 225nm

(89/11); 0.5mL/min; UV

0.5mL/min; UV 278nm

0.8mL/min; UV 210nm

(90/5/5); 1.0 mL/min

77 trichloronate Chiralcel OD heptane; 1.0 mL/min Rs: 1.1 [55]

alcohol(89/11); 0.5mL/min; UV 268.6nm

1.0 mL/min; 2.0 UV 210nm

\*3 CD and OR means signals obtained from circular dichrism detector and optical rotation detector

mL/min; UV 220nm

0.8mL/min; UV 278nm

Chiralcel OJ hexane/IPA(100/5); 1.0

Chiralcel OD-H hexane/IPA(100/2); 1.0

CDMPC hexane/ethanol(99.2/0.8);

Chiralcel OJ-H hexane/IPA(100/3); 1.0

CTMB hexane/n-butyl alcohol

278nm

Lux Cellulose-1 MET/water(60/40); 0.5

Chiralcel OJ heptane; 1.0 mL/min; UV 205nm

Chiralcel OJ hexane/heptane/ethanol

CTPC hexane/ ethanol (93/7); 0.5mL/min; UV 269.8nm

\*1 ACN, MET and IPA means acetronitrile, methanol and isopropanol respectively. \*2 and Rs means the separation factor and the resolution facotr respectively.

**Table 2.** Summary of resolution results of chiral pesticides on cellulose-based CSPs

CTPC hexane/IPA(91/9);

Separation effect\*2

Rs: 2.43, 2.73

Rs: 0.64/2.87/0.3

7

Rs: 0.18/ 0.69/ 0.52

Rs: 1.53/ 0.88

Rs: 1.45/2.73/2.1

6

Elution order\*3

: 1.17 R(-)/S(+) [73]

: 1.81 1R,2S(+)/1S, 2R(-)

: 1.16 1R,2R(+)/1S ,2S(-)

)/1R,2R(+)

[61]

[61]

[61]

OR: (-)- A,/(+)-A/(-)- B/ (+)-B

: 1.0 [81]

Rs: 1.40 [55]

Rs: 4.03 OR: +/- [111]

Rs: 1.45 [61]

Rs: 2.16 [61]

Rs: 1.46 OR: +/- [65]

: 1.03 1S,2S(-

Reference

OR: -/+ [75, 66, 89]

[73]

[73]

[73]

[40]

No. Pesticide CSP or Chiral

Cellulose derivatives have high chiral recognition abilities for racemates and have already become a very popular and useful source material for CSPs. Cellulose-based CSPs can be prepared by coating or bonding cellulose derivatives on decorative silica gel or other supports with various preparation methods. The coated CSPs exhibite higher discrimination abilities for chiral pesticides and are more popular than the bonded CSPs. However, the bonded CSPs can tolerate broader solvent ranges, including THF and CHCl3, which cannot be used on coated CSPs as mobile phases because they have strong dissolution abilities that can dammage or destroy them. Coated CDMPC and CTMB had the broadest application in the stereoselective separations of chiral pesticides. For most pesticides, better separations were obtained on NP-HPLC than on RP-HPLC. However, RP-HPLC can improve the amount of racemates loaded on CSPs as it allows the use of more polar solvents to enhance the solubility of racemates in mobile phases. Additionally, it can be easily connected in tandem with MS, allowing for the development of more sensitive methods for analysis of enantiomers/stereoisomers. The cellulose-based CSPs on NP-HPLC and RP-HPLC provide very powerful tools to prepare individual enantiomers and study the activity, toxicity and environmental fates of chiral pesticides.
