**3. Results and discussion**

12 examples of *trans*-type Schiff base complexes investigated are mentioned below, molecu‐ lar structures [top], crystal structures [middle], and optimized structures [bottom] as spacefilling models with comments.

**Figure 2.** CCDC MIMTOS01 [16].The compound has a formula C34H52CuN4O4

(about 45 degree).

that attaching dialkylaminomethyl arms to commercial phenolic oxime copper extractants yields reagents which transport base metal salt vary efficiently by forming neutral 1:1 or 1:2 complexes with zwitterionic forms of the li‐ gands. Apparently conformational changes were from a square planar geometry to an umbrella form and twist form

2+, 2(NO3 -

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). Novel feature mentioned is

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tionally, we have successfully observed size-dependence of wavelengths of induced CD peaks from chiral Schiff base Zn(II) complexes involving azo-groups at surface plasmon re‐

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As for the induced CD between chiral Schiff base Ni(II) or Zn(II) complexes and Cu-clusters prepared in PAMAM, we have also investigated the role of chiral ligands for molecular rec‐ ognition. For example, naphtylgroups are appropriate for induced CD, while more flexible groups are not [14] (Figure 1). Therefore, several examples indicated that supramolecular or molecular recognition must be a key reason for specific intermolecular interactions. In this review article, we have summarized several examples of crystal structures and optimized structures (as a model of them in solutions) of *trans*-type chiral Schiff base Ni(II), Cu(II), and Zn(II) complexes. In order to derive important steric factors for molecular recognition, we will point out characteristic features of molecular shapes or their conformational changes *in*

**Figure 1.** Examples of suitable [left] and unsuitable [center] ligands for induced CD based on experiments [9, 10, 14].

According to a CCDC database [15], we selected some crystal structures of *trans*-type Schiff base metal complexes. As modeling conformational changes in solutions, we obtain opti‐ mized structures and their heat of formation by using MM2. We will search appropriate fea‐

12 examples of *trans*-type Schiff base complexes investigated are mentioned below, molecu‐ lar structures [top], crystal structures [middle], and optimized structures [bottom] as space-

[Right]Important (bold circles) and unimportant (broken circle)moieties of ligands for induced CD.

gion on colloidal gold particles [13].

*silico*.

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**2. Computation**

tures of molecular shapesfor induced CD.

**3. Results and discussion**

filling models with comments.

**Figure 2.** CCDC MIMTOS01 [16].The compound has a formula C34H52CuN4O4 2+, 2(NO3 - ). Novel feature mentioned is that attaching dialkylaminomethyl arms to commercial phenolic oxime copper extractants yields reagents which transport base metal salt vary efficiently by forming neutral 1:1 or 1:2 complexes with zwitterionic forms of the li‐ gands. Apparently conformational changes were from a square planar geometry to an umbrella form and twist form (about 45 degree).

**Figure 3.** CCDC **MAHYEA** [17].The compound has a formula C30H28CuN2O2. Novel feature mentioned is that it adopts a stepped conformation and displays a square-planar *trans*-[CuN2O2]coordination geometry. The asymmetric unit con‐ tains two independent half molecules and each Cu atom is located on acenter of symmetry.

**Figure 4.** CCDC **MAJNIV** [18].The compound has a formula C34H36CuN2O4. Novel feature mentioned is that com‐ pressed tetrahedral coordination geometry with an *(R,R)*-absolute configuration. These complexes differ from one an‐ other with respect to the 1-phenylethylamine moieties, the direction of the benzene rings being inside and outside of the molecules. Apparently conformational changes were from an umbrella and twist (about 45 degree) form to same and twist (about 90 degree) form. The extended conformation of the phenethylimine pendant groups results in crys‐ tal packing formed by weakly aggregated planar molecules. Apparently conformational changes were from a relative‐

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ly flat step form to a significantly sharp step form.

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**Figure 4.** CCDC **MAJNIV** [18].The compound has a formula C34H36CuN2O4. Novel feature mentioned is that com‐ pressed tetrahedral coordination geometry with an *(R,R)*-absolute configuration. These complexes differ from one an‐ other with respect to the 1-phenylethylamine moieties, the direction of the benzene rings being inside and outside of the molecules. Apparently conformational changes were from an umbrella and twist (about 45 degree) form to same and twist (about 90 degree) form. The extended conformation of the phenethylimine pendant groups results in crys‐ tal packing formed by weakly aggregated planar molecules. Apparently conformational changes were from a relative‐ ly flat step form to a significantly sharp step form.

**Figure 3.** CCDC **MAHYEA** [17].The compound has a formula C30H28CuN2O2. Novel feature mentioned is that it adopts a stepped conformation and displays a square-planar *trans*-[CuN2O2]coordination geometry. The asymmetric unit con‐

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tains two independent half molecules and each Cu atom is located on acenter of symmetry.

**Figure 5.** CCDC **MIZGIM** [19].The compound has a formula C30H24CuN2O4. Novel feature mentioned is that the coordi‐ nation geometry around the copper atom in the complex is intermediate between square-planar and tetrahedral with two salicylaldimine ligands in trans arrangement. The molecular chains are linked via additional C-H⋅⋅⋅⋅O hydrogen bonds to form a three-dimensional supramolecular network. Apparently conformational changes were from a moder‐ ately umbrella and slightly twist form to a twist (about 90 degree) form.

**Figure 6.** CCDC **IBHBCU01** [20].The compound has a formulaC34H34Cl2CuN2O2. Novel feature mentioned is that the isobutyl complex exists in two distinct crystalline forms, green and red. The green isomerhas the isobutyl groups point‐ ing to the same side of the approximate [CuO2N2] plane. The red isomer of the isobutyl complex contains two crystal‐ lographically independent molecules having the isobutyl groups. Apparently conformational changes was from a step

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form to an umbrella and twist (about 90 degree) form.

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**Figure 6.** CCDC **IBHBCU01** [20].The compound has a formulaC34H34Cl2CuN2O2. Novel feature mentioned is that the isobutyl complex exists in two distinct crystalline forms, green and red. The green isomerhas the isobutyl groups point‐ ing to the same side of the approximate [CuO2N2] plane. The red isomer of the isobutyl complex contains two crystal‐ lographically independent molecules having the isobutyl groups. Apparently conformational changes was from a step form to an umbrella and twist (about 90 degree) form.

**Figure 5.** CCDC **MIZGIM** [19].The compound has a formula C30H24CuN2O4. Novel feature mentioned is that the coordi‐ nation geometry around the copper atom in the complex is intermediate between square-planar and tetrahedral with two salicylaldimine ligands in trans arrangement. The molecular chains are linked via additional C-H⋅⋅⋅⋅O hydrogen bonds to form a three-dimensional supramolecular network. Apparently conformational changes were from a moder‐

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ately umbrella and slightly twist form to a twist (about 90 degree) form.

**Figure 7.** CCDC **DPESCU11** [21].The compound has a formula C30H28CuN2O2. Novel feature mentioned is that cop‐ per(II) complexes of three chiral enantiomeric pairs of o-hydroxy Schiff bases derived from (R)-(+)-1-phenylethylami‐ neand/or (S)-(-)-1-phenylethylamine, were prepared and characterized.The geometry around the metal atom is distorted square planar. Apparently conformational change was from a twist (about 45 degree) form to a twist (about 90 degree) form.

**Figure 8.** CCDC **MSACOP12** [22].The compound has a formula C16H16CuN2O2. Novel feature mentioned is that a di‐ meric molecule in which monomeric halves is joined by two Cu-O bondsto complete a square-pyramidal configuration about each copper atom. Distortions in the molecule are evidentlydue to the close approach of non-bonding regions. It is now seen that this compound displays three differentcoordination arrangements in its three polymorphic forms.

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Apparently conformational change was from a step form to an umbrella and twist (about 45 degree) form.

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**Figure 8.** CCDC **MSACOP12** [22].The compound has a formula C16H16CuN2O2. Novel feature mentioned is that a di‐ meric molecule in which monomeric halves is joined by two Cu-O bondsto complete a square-pyramidal configuration about each copper atom. Distortions in the molecule are evidentlydue to the close approach of non-bonding regions. It is now seen that this compound displays three differentcoordination arrangements in its three polymorphic forms. Apparently conformational change was from a step form to an umbrella and twist (about 45 degree) form.

**Figure 7.** CCDC **DPESCU11** [21].The compound has a formula C30H28CuN2O2. Novel feature mentioned is that cop‐ per(II) complexes of three chiral enantiomeric pairs of o-hydroxy Schiff bases derived from (R)-(+)-1-phenylethylami‐ neand/or (S)-(-)-1-phenylethylamine, were prepared and characterized.The geometry around the metal atom is distorted square planar. Apparently conformational change was from a twist (about 45 degree) form to a twist (about

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**Figure 9.** CCDC **MAJCUW** [23].The compound has a formula C22H24Cl4CuN2O2. Novel feature mentioned is that it has a compressed tetrahedral *trans*-[CuN2O2] coordination environment with an umbrella conformation of the overall mole‐ cule.The absolute configuration is found to be *(S,S)* for the crystalexamined.Molecular recognition for the chiral mole‐ cules could not be carried out using hydrogen bonding because of no possible hydrogen bonding sites in the crystal packing. Apparently conformational change was from an umbrella and twist form to a twist (about 45 degree) form.

**Figure 10.** CCDC **KUPBIH** [24].The compound has a formula C30H32CuN2O2. Novel feature mentioned is that correla‐ tion between the bulkiness of the imine nitrogensubstituent, deformation of the copper coordination sphere is impor‐ tant and tBu group in the *N*-tBu derivative prevents such dynamic action. In the crystal, this *N*-tBu complex changes upon DFT geometry optimization to a more tetrahedralconfiguration. Apparently conformational change was from an

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umbrella and slightly twists form to an umbrella and twist (about 90 degree) form.

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**Figure 10.** CCDC **KUPBIH** [24].The compound has a formula C30H32CuN2O2. Novel feature mentioned is that correla‐ tion between the bulkiness of the imine nitrogensubstituent, deformation of the copper coordination sphere is impor‐ tant and tBu group in the *N*-tBu derivative prevents such dynamic action. In the crystal, this *N*-tBu complex changes upon DFT geometry optimization to a more tetrahedralconfiguration. Apparently conformational change was from an umbrella and slightly twists form to an umbrella and twist (about 90 degree) form.

**Figure 9.** CCDC **MAJCUW** [23].The compound has a formula C22H24Cl4CuN2O2. Novel feature mentioned is that it has a compressed tetrahedral *trans*-[CuN2O2] coordination environment with an umbrella conformation of the overall mole‐ cule.The absolute configuration is found to be *(S,S)* for the crystalexamined.Molecular recognition for the chiral mole‐ cules could not be carried out using hydrogen bonding because of no possible hydrogen bonding sites in the crystal packing. Apparently conformational change was from an umbrella and twist form to a twist (about 45 degree) form.

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**Figure 11.** CCDC **KUPBON** [24].The compound has a formula C26H24CuN2O2. Novel feature mentioned is that the coor‐ dination sphere of the N-ethyl derivative has a flat-tetrahedral geometry. TheN–Cu–Nand O–Cu–O angles and the di‐ hedral angle betweenthe planes N–Cu–O and N–Cu–Oin the solid state found by X-ray diffraction in this study are affected by crystalpacking forces according to these DFT calculations. Apparently conformational change was from a flat and square planar form to an umbrella and V-shaped form drastically.

**Figure 12.** CCDC **YUBLAJ** [23].The compound has a formula C66H88Br2Cu3N6O2. Novel feature mentioned is that it ap‐ pears that problematic deprotonation of the phenol to give a chelating or bridging ligand is the primary reason for the observed instability based on the stability of related copper NHC–aryl oxide compounds (including mixed valence Cu(I)/Cu(II) centers Cu(I) sites in ligands) Apparently conformational change was from a step and slightly twist form to

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an umbrella and twist (about 90 degree) form.

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**Figure 12.** CCDC **YUBLAJ** [23].The compound has a formula C66H88Br2Cu3N6O2. Novel feature mentioned is that it ap‐ pears that problematic deprotonation of the phenol to give a chelating or bridging ligand is the primary reason for the observed instability based on the stability of related copper NHC–aryl oxide compounds (including mixed valence Cu(I)/Cu(II) centers Cu(I) sites in ligands) Apparently conformational change was from a step and slightly twist form to an umbrella and twist (about 90 degree) form.

**Figure 11.** CCDC **KUPBON** [24].The compound has a formula C26H24CuN2O2. Novel feature mentioned is that the coor‐ dination sphere of the N-ethyl derivative has a flat-tetrahedral geometry. TheN–Cu–Nand O–Cu–O angles and the di‐ hedral angle betweenthe planes N–Cu–O and N–Cu–Oin the solid state found by X-ray diffraction in this study are affected by crystalpacking forces according to these DFT calculations. Apparently conformational change was from a

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flat and square planar form to an umbrella and V-shaped form drastically.

> may not be an important factor for it. However, the experimental facts that only complexes with specific ligands or metal ions (which determine their coordination geometries) suggest‐ ed that induced CD appears under appropriate steric (as well as stereochemical) conditions for metal complexes. One of the important factors of steric factors for metal complexes may be distance between (electric) dipole moments at the surface achiral materials which keep their shapes rigidly. The reason for this assumption is that both metallodendrimers metal and nanoparticles have approximately spherical shapes essentially even surrounded in

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As for biomolecules such as proteins, however, CD spectra are used for monitoring folding or unfolding of peptide chains after binding small molecules of metal complexes [25]. This different phenomenon is not classified into the induced CD mentioned in this article. By in‐ cluding small molecules into proteins with weakly supramolecular forces, molecules of pro‐ teins change their molecular conformation, which attributed to shift of strong π−π\* bands of C=O moieties electronic or CD spectra. This docking mechanism is directly molecular recog‐ nition accompanying with conformational changes of proteins as well as small molecules, which is also confirmed by quenching of fluorescence intensity due to energy transfer.

In contrast, non-contact interactions of (electric) dipole moments for CD spectra have com‐ plicated problems. Our preliminary results of CD spectra of chiral Schiff base metal com‐ plexes in viscous solutions dissolved a certain protein exhibited concentration dependence of so-called artifact peaks of solid-state CD spectra [26]. The artifact CD peaks are attributed to anisotropic molecular orientation and removed in matrix environment which permits mo‐ lecular rotation isotropically accompanying with (magnetic) dipole moments of chiral mole‐ cules [27]. Therefore,not only CD spectra of chiral molecules in anisotropically oriented matrix such as biomolecules but also induced CD bands involving softmaters is still an open

As summarized in Figure 1[right], according to chemical structures, Zn(II) center and naph‐ tylgroups are suitable factors for induced CD, while 3,5-dichlorosalycilaldehyde moieties are not regardless of common factors. Previous study [11] revealed that in optimized struc‐ ture, naphtylgroups act as largely spread planar parts outside of a molecular face, which plays an important role in induced CD for this case. In the present study, compounds hav‐ ing identical features were also investigated in view of optimized structures. According to not only3,5-dichlorosalycilaldehyde moieties (**IBHBCU01** and **MAJCUW**) but also tert-Bugroups (**MIMTOS01** and **YUBLAJ**), EtO- groups (**MAJNIV**), and NO2- groups (**METSUZ**) gave significantly large steric hindrance resulting in steric repulsion between ligands. How‐ ever, specific geometry could not be induced by bulky groups. Generally, Zn(II) complexes afford a tetrahedral coordination geometry, which prevents from forming flatten planar mo‐ lecular shapes in view of ligands. Therefore, these two factors may not be definitive factors solely. On the other hand, besides in amine parts (Figure 1), naphtylgroups in aldehyde

softmaters.

question.

**4. Conclusion**

**Figure 13.** CCDC **METSUZ** [24].The compound has a formula C30H26N4O6Zn. Novel feature mentioned is that it crystalli‐ zes in the noncentrosymmetric space groups. The geometry around the Zn(II) metalcenter is pseudo-tetrahedral with two oxygen and two nitrogen atoms from the ligands and has the Λ absoluteconfiguration. Apparently conformation‐ al change was slight, namely it remained a twist (about 90 degree) from.

In principle, induced CD is caused by non-contact interactions between (electric) dipole mo‐ ments of chiral additives and achiral materials. Because it is an electromagnetic phenomen‐ on essentially, contact intermolecular interactions, in other words molecular recognition, may not be an important factor for it. However, the experimental facts that only complexes with specific ligands or metal ions (which determine their coordination geometries) suggest‐ ed that induced CD appears under appropriate steric (as well as stereochemical) conditions for metal complexes. One of the important factors of steric factors for metal complexes may be distance between (electric) dipole moments at the surface achiral materials which keep their shapes rigidly. The reason for this assumption is that both metallodendrimers metal and nanoparticles have approximately spherical shapes essentially even surrounded in softmaters.

As for biomolecules such as proteins, however, CD spectra are used for monitoring folding or unfolding of peptide chains after binding small molecules of metal complexes [25]. This different phenomenon is not classified into the induced CD mentioned in this article. By in‐ cluding small molecules into proteins with weakly supramolecular forces, molecules of pro‐ teins change their molecular conformation, which attributed to shift of strong π−π\* bands of C=O moieties electronic or CD spectra. This docking mechanism is directly molecular recog‐ nition accompanying with conformational changes of proteins as well as small molecules, which is also confirmed by quenching of fluorescence intensity due to energy transfer.

In contrast, non-contact interactions of (electric) dipole moments for CD spectra have com‐ plicated problems. Our preliminary results of CD spectra of chiral Schiff base metal com‐ plexes in viscous solutions dissolved a certain protein exhibited concentration dependence of so-called artifact peaks of solid-state CD spectra [26]. The artifact CD peaks are attributed to anisotropic molecular orientation and removed in matrix environment which permits mo‐ lecular rotation isotropically accompanying with (magnetic) dipole moments of chiral mole‐ cules [27]. Therefore,not only CD spectra of chiral molecules in anisotropically oriented matrix such as biomolecules but also induced CD bands involving softmaters is still an open question.
