**4.3. Fluorescently-labeled peptide substrates for palmitoylation**

Fluorescently-labeled peptides that mimic PAT substrates have been used to characterize PAT activity and for the discovery of inhibitors of palmitoylation [123, 126, 127]. The use of these peptides over the last several years was reviewed recently [123]. Peptide substrates for palmitoylation have also been genetically fused to fluorescent proteins and expressed in cells. This strategy has been used to determine how palmitoylation affects subcellular traf‐ ficking both between and within membranes [124]. Monomeric GFP-based reporters and fluorescence resonance energy transfer proved to be helpful in the identification of lipid rafts with an affinity for palmitate on the inner leaflet of the plasma membrane [5].
