**7. Other applications**

SRAP amplification is actually a small portion of all possible sampling of a genome. So SRAP can be used to produce a reduced genome samples when multiple SRAP reactions are pooled. As described previously, pooled SRAP produces can be directly sequenced using next generation sequencing technologies. When replacing genomic DNA with cDNA samples, SRAP is adequate to perform gene expression profiling and also con‐ struct cDNA genetic maps.

More recently, Yu et al., (2012) used SRAP markers to distinguish fertile somatic hybrids of *G. hirsutum L.* and *G. trilobum* produced by protoplast fusion. They obtained fertile somatic hybrids by symmetric electrofusion of protoplasts of tetraploid upland cotton *G. hirsutum* and wild cotton *G. trilobum.* These hybrids were confirmed using morphological characteris‐ tics, flow cytometric analysis, and molecular markers including RAPD, SRAP and AFLP.

In aquaculture, Ding et al., (2010) used SRAP and SCAR markers to differentiate two cul‐ tured populations in grass carp (*Ctenopharyngodon idella*). Through cloning and sequencing SRAP fragments, they developed SCAR markers to characterize individuals from the cul‐ tured population and the wild population, showing different frequencies of SCAR alleles (87% in the cultured population and 6% in the wild), suggesting that this SCAR might serve as a specific molecular marker for the cultured population. They also identified eight SRAP fragments that shared high similarities to functional genes.
