**2.2 Protein concentration determination**

Sera protein concentration was determined by Bradford assay method, using bovine serum albumin (BSA) as standard. Known amounts of BSA were diluted in 800 μL of H2O and then mixed to 200 μL of Comassie Brilliant Blue. 5 different BSA concentrations were determined by measuring absorbance at 595 nm and used to obtain a linear calibration curve. Three different sera dilutions were measured at 595 nm. Absorbance data were interpoled on the calibration curve, allowing the determination of protein concentration in the different samples.
