: *E. coli malE* gene is used as a negative control.

**Table 3. Summary of RNAi for 12 GPCRs in** *T. castaneum*. Asterisk indicates that RNAi for TC012493 was not carried out at the second screen.

#### **4. Conclusion**

106 Functional Genomics

showing any ecdysis defects.

classical GPCRs [73].

**Class A Rhodopsin-like** 

**Class B Secretin receptorlike** 

**Class D Atypical GPCRs** 
