**4.5. Our further direction**

*In silico* approach offers various advantages over *in vivo* and *in vitro* approaches such as non-use of animals, low costs, and reduced execution time. This approach allows identification of proteins of interest from a particular biological study. From a protein function standpoint, transfer of annotation from known proteins to a novel target is currently the only practical way to convert vast quantities of raw sequence data into meaningful information. Many bioinformatics tools now provide more sophisticated methods to transfer functional annotation, integrating sequence, family profile and structural search methodology. Thus, in addition to data mining for protein-protein interaction, further *in silico* approach should also consider structural alignment, molecular docking and pathway modeling in order to obtain a comprehensive and more reliable insight into protein-protein interaction of *T. brucei* nuclear transport.

Investigation on Nuclear Transport of *Trypanosoma brucei*: An *in silico* Approach 51

and Umi Marshida Abdul Hamid

*School of Biology, Faculty of Applied Sciences, MARA University of Technology Shah Alam, Shah* 

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**Author details** 

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 \*

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*Alam Selangor, Malaysia* 

Mohd Fakharul Zaman Raja Yahya\*
