**1. Introduction**

It is known that red blood cells release ATP when blood oxygen tension decreases. ATP has an effect on microvascular endothelial cells to form a retrospective conducted vasodilation to the upstream arteriole. Local metabolic control of coronary blood flow due to vasodilation in microvascular units where myocardial oxygen extraction is relatively high occurs due to ATP.[5] Arterioles dilate or constrict in response to changing intravascular pressure.[6]

"It is well known that myogenic responses, ow-dependent vasodilation, local metabolic effects, and propagation all contribute to blood ow regulation. Primarily responsible for carrying oxygen in blood, red blood cells (RBCs) may also act as oxygen sensors and thus play a role in the communication of metabolic demand" [3,7]. The mechanisms for release of ATP from the RBC in response to lowered oxygen saturation have been studied. [8]. Jagger et al. [9] measured the ATP release at low O2 levels in the presence and absence of CO to demonstrate that the release of ATP from RBCs may be connected to the change of the hemoglobin molecule from its relaxed state to its deoxygenated state. Upon release, ATP binds to P2Y purinergic receptors on the luminal surface of the endothelium, starting the conducted response [10].An in vitro microfluidic experimental study to investigate the dynamics of shear-induced ATP release from human RBCs with millisecond resolution was conducted by Wan et al. [11].Conclusively it was shown that there is a sizable delay time between the onset of increased shear stress and the release of ATP. "It was seen that this response time decreases with shear stress, but does not depend significantly on membrane rigidity. It was shown that even though the RBCs deform significantly in short constrictions (duration of increased stress <3 ms), no measurable ATP is released."[11]

© 2012 Moschandreou, licensee InTech. This is an open access chapter distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. © 2012 Moschandreou, licensee InTech. This is a paper distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. © 2012 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

ATP is short for adenosine triposphate, which is a "currency" of biological energy. There exists an adenosine group with 3 phosphate groups attached to it. Hydrolizing this bond detaches one of the phosphate groups and produces ADP, which is adenosine di-phospahte plus a phosphate group and energy. Through chemical reaction if you pop off a phosphate group of ATP, energy will be generated for general heat or one can couple this reaction with other reactions that require energy. This chemical reaction of ATP to ADP involves going from stored energy to used energy. ADP can be recharged back to ATP by processes in the mitochondria.

RBC-ATP Theory of Regulation for Tissue Oxygenation-ATP Concentration Model 157

**Figure 2.** Ball and Stick Model of ATP based x-ray diffraction data[13]

**Figure 1.** Adenosine Triphosphate[12]

The same part(adenine) that makes up DNA is that which makes up these energy currency molecules known as ATP molecules. Adenine makes part of adenosine which makes part of ATP. The other part of ATP is known as ribose from RNA, which is a five carbon sugar. ATP drives biological reactions. In terms of electrons when one pops off the phosphate group the electrons enter a lower energy state between phosphate and oxygen atoms which generates energy.

RBC's have no nucleus or mitochondria. As a result RBC's obtain their energy using glycolysis to produce ATP. There are both advantages and disadvantages to this. An advantage is due to the biconcave disk shape which optimizes the cell for the exchange of oxygen with its surroundings and optimizes space for the hemoglobin. The RBC's are deformable and flexible so that they can move through the tiny capillaries where oxygen is released. The disadvantage is that because of the absence of nuclei and organelles, mature RBC's do not contain DNA and cannot synthesize any RNA, and cannot divide or repair themselves. The Mitochondria enables cells to produce 15 times more ATP than usual. Lack of mitochondria means that the cells use none of the oxygen they transport. Instead they produce the energy carrier ATP by means of fermentation, via glycolysis of glucose and by lactic acid production.

**Figure 1.** Adenosine Triphosphate[12]

energy.

lactic acid production.

mitochondria.

ATP is short for adenosine triposphate, which is a "currency" of biological energy. There exists an adenosine group with 3 phosphate groups attached to it. Hydrolizing this bond detaches one of the phosphate groups and produces ADP, which is adenosine di-phospahte plus a phosphate group and energy. Through chemical reaction if you pop off a phosphate group of ATP, energy will be generated for general heat or one can couple this reaction with other reactions that require energy. This chemical reaction of ATP to ADP involves going from stored energy to used energy. ADP can be recharged back to ATP by processes in the

The same part(adenine) that makes up DNA is that which makes up these energy currency molecules known as ATP molecules. Adenine makes part of adenosine which makes part of ATP. The other part of ATP is known as ribose from RNA, which is a five carbon sugar. ATP drives biological reactions. In terms of electrons when one pops off the phosphate group the electrons enter a lower energy state between phosphate and oxygen atoms which generates

RBC's have no nucleus or mitochondria. As a result RBC's obtain their energy using glycolysis to produce ATP. There are both advantages and disadvantages to this. An advantage is due to the biconcave disk shape which optimizes the cell for the exchange of oxygen with its surroundings and optimizes space for the hemoglobin. The RBC's are deformable and flexible so that they can move through the tiny capillaries where oxygen is released. The disadvantage is that because of the absence of nuclei and organelles, mature RBC's do not contain DNA and cannot synthesize any RNA, and cannot divide or repair themselves. The Mitochondria enables cells to produce 15 times more ATP than usual. Lack of mitochondria means that the cells use none of the oxygen they transport. Instead they produce the energy carrier ATP by means of fermentation, via glycolysis of glucose and by

**Figure 2.** Ball and Stick Model of ATP based x-ray diffraction data[13]

RBC-ATP Theory of Regulation for Tissue Oxygenation-ATP Concentration Model 159

**2. Structural features erythrocyte and the erythrocyte membrane** 

mitosis.

hemoglobin.

spherical.

lysolecithin or fatty acids.

**4. Dimensions of RBC** 

**5. Present objective** 

concentration of ATP is thus affected.

**3. Shape of RBC** 

Lacking organelles as nucleus, mitochondria, or ribosomes, the red cell does not synthesize new proteins, carrying out the oxidative reactions associated with mitochondria, or undergo

The RBC consists of a membrane surrounding a solution of protein and electrolytes. About 95% of the protein is the oxygen-transport protein, hemoglobin. The remainder of the protein includes enzymes required for energy production and for maintenance of

In immobile state, the normal human RBC is shaped as a biconcave disc. The disc shape is important to erythrocyte function. The ratio of surface to volume is optimized so that oxygen transfer is possible. Also the biconcave disc is more deformable than a sphere and

The four possible forces to maintain the shape described are (1) elastic forces within the membrane, (2) surface tension, (3) electrical forces on the membrane surface, and (4) osmotic or hydrostatic pressures. The maintenance of RBC shape is dependent on the structure of the cell as well as in the external environment. If these are changed, the cell may become

When RBC's are suspended in hypotonic solutions andosmotic swelling occurs. This can make the cell spherical. These changes are associated with an increase in volume while the cell surface area remains the same or changes only slightly. When spherical shape is attained, the cell diameter decreases, and this shows the elastic properties of the membrane.

Discocyte-echinocyte transformation takes place when ATP is depleted, when intracellular calcium is increased, when the cell is exposed to plasma, anionic detergents, high pH,

Photomicrographs are used to measure the dimensions of RBC's. Average values for the mean cellular volume in normal subjects are from approximately 85 to 91 fl. Ninety-five percent of normal red cells are between about 60 and 120 fl in volume. Various results have

In this work we model the time delay of release of ATP as supporting work shows by Wan et al. [11] for shear-induced ATP release from red blood cells. A release rate which is a function of time and introduces a delay mechanism is introduced to show how the

yielded an average normal value for red cell diameter of 7.2 to 7.4 microns.

undergoes the change in shape necessary for optimal movement in microvasculature.

**Figure 3.** Space-Fill Representation of ATP[14]
