*5.2.2. Scenario of the system in clinical environment*

Patients' blood samples will be collected on anticoagulant. RBC and plasma are then separated in different tubes. Plasma is used as a ligand in immune-affinity chromatography to separate hemolysate antigens that can bind to plasma antibodies. The separated antigens are identified by MS and stored in the database indexed by the patient disorder.

In the same time, queries are done to verify the diagnosis and get a prognosis and a recommended treatment component. The following formulas describe the usage of this model in clinical practice.

Let Dp' is the set of all discovered P'dj Let Dp'' is the set of all discovered P''dj

Then

26 Blood Cell – An Overview of Studies in Hematology

**Figure 10.** Venn diagram depicting set of abnormal protein of disease X (P'dj)

b. Model of Diseases caused as a result of missed tissue proteins

The result is the set which contains all proteins associated with dj

**Figure 11.** Venn diagram depicting set of missed normal proteins of disease Y (P''dj)

Those diseases can be caused due to existence of abnormal protein or absence of tissue

with the proteins that give positive reaction, Figure 11.

c. Model of Diseases of unknown cause (Idiopathic)

proteins.

There are many diseases that are identified as idiopathic.

Pudj = ∪{Pp}dj

P''dj = Pnormal - Pudj

The result is the set which contains proteins that can be used to diagnose patients through detecting circulating auto-antibodies and to treat those patients through desensitizing them ∀ P'dj ∈ Dp', if P'dj ⊂ P, then patient is diagnosed to have dj

Else

P''dj ∈ Dp'', if P''dj P, then patient is diagnosed to have dj
