**6. Conclusion**

298 Blood Cell – An Overview of Studies in Hematology

[9].

13.

after implantation was significantly suppressed by whole-body FIR starting from day 15, compared to the control group (to refer J Table1). The cDNA microarray analysis of tumor samples of control and FIR-treated showed that MMP-1, MMP-9, MMP-10, and MMP-13 in the MMP family were significantly down-regulated in the FIR group compared with control group (to refer ITEL 2005, 6(6), 597-601, Table1) [9]. The expression profiles obtained from cDNA microarray analyses as analyzed by quantitative real-time RT-PCR showed the control group to have highly significant overexpression of the genes for MMP-1, MMP-9, MMP-10, and MMP-13 compared to the FIR group (to refer ITEL 2005, 6(6), 597-601, Fig.4)

These results suggest that suppression of tumor volume increase by in vivo FIR radiation was due to inhibition of MMPs by FIR radiation. In other words, FIR suppresses invasion

As shown in (to refer ITEL 2005, 6(6), 597-601, Fig 5) [9], extensive portions of tumor tissue in the FIR group was encapsulated and necrotized in intra tumor division. On the other hand, in the control group tumor cells showed active proliferation and invasion into the surrounding muscular tissue. In addition, evaluation of the immunohistochemical expression of MMP-1, MMP-9, MMP-10 and MMP-13 using tumor tissues of control and FIR groups on day 30 after implantation showed MMP-1 and MMP-9 to be positively expressed in the tumor stroma of the control group, while MMP-10 and MMP-13 were strongly positive in tumor parenchyma (the part positively dyed at Cytokeratin10) (to refer ITEL 2005, 6(6), 597-601, Fig. 6) [9]. However, these MMPs in the FIR group were not significantly expressed. The result of immunohistological detection for these MMPs was concordant with the results of cDNA microarray and QRT-PCR. That is, FIR radiation seemed to suppress invasion of tumor cells by inhibiting the expression of MMP-1, MMP-9, MMP-10, and MMP-

The increased expression and activity of MMPs is associated with tumor invasion, metastasis, and angiogenesis [22]. The role of MMP-1 in tumor invasion and metastasis has only recently been determined [23]. MMP-1 has been shown to cleave entactin, thus contributing to the degradation of basement membranes and hence potentially contributing to the transitioning across epithelial barriers by tumor cells [24]. Immunohistochemical detection of MMP-1 expression is also associated with increased invasive potential and poor prognosis in colon and esophageal cancers [25, 26]. The first barrier to tumor invasion is the basement membrane, and because one of its principle constituents is type I collagen, the gelatinases (MMP-2 and MMP-9) are thought to play important roles in its degradation [27]. MMP-10 (stromelysin-2) is known to degrade various components of the extracellular matrix, and though it has been reported that MMP-10 (stromelysin-2) expression by lymphoma cells accelerates the growth of thymic lymphoma [28], the role of MMP-10 in other types of tumor growth is relatively unknown. MMP-13 (collagenase-3) is expressed in breast carcinoma and in articular cartilage of arthritic patients [29]. In addition, MMP-13 has high collagenolytic and gelatinolytic activity, and MMP-13 may be of importance in

and metastasis of tumor cells by inhibiting the expression of MMP-1, 9, 10 and 13.

It was made clear that the motion of the water molecules was surely and physically activated by the radiation of FIR with the CO2 incubator and the animal raising apparatus. The analysis of the change of blood circulation conducted the conclusion that the FIR energy radiation activated not only the motion of water molecules but also the blood circulation in the living body. Still more, it was made clear that the activation effects on the water molecules developed the activation effects of the skin regeneration and the new bone formation. Moreover, the proliferation or metastasis of the various cancer cells were inhibited by the FIR energy radiation connected with the activation of blood circulation. It is expected that the present studies on the FIR energy radiation connect with the development of medical treatment for the regeneration of the tissue and organ as a skin and a bone, and the prevention medicine for cancer.
