**5. Results**

Closed follicular architecture is indispensable in agarose culture since open follicles become dissociated and cells die; it is thus essential to begin with maximum closed follicles possible to avoid this. The following item gives the criteria determined important for beginning culture. Then, the long-term culture results in which closed and isolated follicle morphology and function were compared. Last, the morphological and functional results in the presence of different doses of iodide and TSH.
