**11. References**


<sup>\*</sup> Corresponding Author

[4] Laus MC, Logman TJ, Lamers GE, Van Brussel AA, Carlson RW, Kijne JW (2006) A Novel Polar Surface Polysaccharide from *Rhizobium leguminosarum* Binds Host Plant Lectin. Mol. Microbiol. 59: 1704-1713.

118 The Complex World of Polysaccharides

The EPSs described in this review have common structure in that their repeating units possess identical backbone chains and the same 1-6-linked glucosyl residue starting the side chain. The diversity of EPSs is specified by the structure of their side chains. Presumably, structural information contained in side chains determines participation of EPS in symbiosis as signaling factors. This assumption follows from at least the observation that the absence of a single pyruvyl group in the side chain dramatically disturbed symbiotic

Most of the *R*. *leguminosarum* and *R*. *etli* genes involved in EPS biosynthesis are localized within the single cluster Pss-I. Gene arrangement in this cluster is similar. This implies that Pss-I clusters of these bacterial species have been evolved from a common ancestor. The differences mostly include genetic rearrangements of GTs genes, genes for modification of certain glycosyl residues in the side chain, and genes *pssL*/*psaI* thought to be involved in controlling translocation of the repeating units. It seems that each variant of Pss-I cluster

*Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, Russia* 

We are thankful to Yu.A. Knirel for providing an information dealing with rhizobia EPS structures and A.S. Glukhov, T.B. Kuvshinkina and L.A. Kulakov for the help in the manuscript preparation. This work was supported by grant 11-04-02120a from the Russian

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Vladimir N. Ksenzenko

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