**3.4. Statistical analysis**

*In vivo* and *in vitro* experiments were performed at least three separate times. All statistical analyses were performed with GraphPad prism 4.0a Software (GraphPad Software Inc., USA). Data were presented as the mean ± standard deviation (SD) of triplicates from three independent experiments. Data sets with multiple comparisons were evaluated by one-way analysis of variance (ANOVA) with Dunnett's *post-hoc* test. *P*<0.05 was considered to be statistically significant.
