**2.6. Detection of amino acid substitutions related to IRRDR in NS5A of HCV-G1b**

Nucleotide sequences of NS5A-IRRDR were analyzed by direct sequencing after doubleround PCR according to El-Shamy et al. [34] and the numbers of aa substitutions within this region (2334-2376) determined. Moreover, the particular position of aa substitutions were evaluated in connection to dyslipoproteinemia, found in chronic HCV-G1b patients.
