**3. Results and discussion**

#### **3.1. PA content increases after SA infiltration**

Because of its central position in the pathways mentioned above, the first results presented here have been obtained for PA. Table 1 shows the variations in PA levels in SA-infiltrated leaves, compared to the control. No change in PA content was observed during the first 24 hours after infiltration (hai) of SA, compared to the water-infiltrated wheat leaves; even though a slight accumulation of PA was observed in water-infiltrated leaves in comparison to the untreated plants, probably due to the stress generated by the infiltration. However, SA induced increases in PA content from 24 h till 96 hai, with a maximum of 6.2-fold increase at 72 hai.

Lipids as Markers of Induced Resistance in Wheat: A Biochemical and Molecular Approach 371

**3.2.** *PLC* **gene expression is up-regulated and DAG content increases in SA-**

**Figure 5.** *PLC* gene expression in wheat leaves infiltrated with SA

In order to corroborate the PA formation with the PLC-DGK pathway activation, the expression of the *PLC* gene, encoding a phospholipase C, was measured over the timecourse experiment, compared to the water-infiltrated wheat leaves, and normalized to two reference genes, *tub* and *ef1α*, encoding tubulin and elongation factor, respectively (Figure 5). The expression pattern of the *PLC* gene consisted of three up-regulations: 3.5 and 4.8-fold increases were induced at 9 and 21 hai, respectively. Furthermore, this gene expression was strongly increased from 48 till 96 hai, with an average of 9-fold increase over this period. This late high up-regulation of *PLC* gene correlates with the late PA detection in wheat leaves between 48 and 96 h after SA infiltration. The accumulation of PA is probably due to this pathway's stimulation after *PLC* gene's expression and synthesis of the corresponding

PA formation through the phospholipase C pathway results from two enzymes acting sequentially: PLC hydrolyses phosphatidylinositol-4,5-bisphosphate [PtdIns(4,5)P2, also abbreviated as PIP2] into inositol-1,4,5-trisphosphate [Ins(1,4,5)P3] and DAG. DAG remains in the membrane and is rapidly phosphorylated to PA by DGK (Figure 1). The variation in DAG levels in SA-infiltrated wheat leaves is presented in table 2. During the first 24 h after SA infiltration, no clear variation pattern in DAG content was observed. However, SA induced the accumulation of DAG from 24 till 96 hai, with a maximum of 2.18-fold increase at 72hai. Interestingly, DAG accumulation, as well as *PLC* gene expression, was recorded in the same period of the time-course experiment, 24 till 96 h after SA infiltration. The DAG

accumulation seems to be the consequence of the induction of *PLC* gene expression.

Twenty four hours after infiltration, SA induces the expression of PLC-encoding gene, simultaneously with an accumulation of DAG and PA. One could think that DAG content must decrease in order to fulfill PA formation; indeed, the contribution of DAG could only

**infiltrated leaves** 

enzyme.


**Table 1.** Variations in PA levels in SA-infiltrated wheat leaves compared to the water-infiltrated control

These results confirm some variations in PA content reported by several authors. Treatment of *A. thaliana* protoplasts with H2O2 increases PA content by 30% [52]. Furthermore, elicitors from plant pathogens activate the PLC-DGK pathway, which consisted of a rapid accumulation of PA within 2 minutes in transgenic tobacco cells treated with the race-specific elicitor Avr4 [53]. A transient accumulation of PA was also recorded in suspension-cultured tomato cells treated with the general elicitors N,N',N'',N'''-tetraacetyl-chitotetraose, xylanase, and the flagellin-derived peptide flg22 [54]. In rice cells, the PA amount increased rapidly after treatment with Nacetylchitooligosaccharide elicitor [55]. Moreover, the PA increase is likely to occur upstream of the oxidative burst [53,55]. Furthermore, method of PA assessment. Furthermore, all these studies point out the rapid accumulation of PA upon treatments, generally within minutes. According to [16], signaling lipids, unlike structural lipids, are present only in minute amounts, yet their levels increase rapidly in response to certain stimuli. Such an accumulation is transient because the signal is rapidly down regulated. However, none of these characteristics, namely the rapid and transient accumulation upon treatment, met our results. SA induces a PA accumulation that occurred not earlier than 24 h after SA infiltration and seemed to last for at least 4 days. This result, that does not match the general trend, may be explained by a late induction of one or both of the phospholipases pathways leading to PA formation. Since the magnitude of PA change varies upon the treatment, tissue and method of PA assessment [17], our findings could be attributed to the treatment and/or to the tissue nature - infiltration of SA and PA assessment *in planta* - whereas most of the studies are conducted on cellular cultures.

#### **3.2.** *PLC* **gene expression is up-regulated and DAG content increases in SAinfiltrated leaves**

370 Lipid Metabolism

increase at 72 hai.

**3. Results and discussion** 

PA content 2.2-fold

**3.1. PA content increases after SA infiltration** 

Because of its central position in the pathways mentioned above, the first results presented here have been obtained for PA. Table 1 shows the variations in PA levels in SA-infiltrated leaves, compared to the control. No change in PA content was observed during the first 24 hours after infiltration (hai) of SA, compared to the water-infiltrated wheat leaves; even though a slight accumulation of PA was observed in water-infiltrated leaves in comparison to the untreated plants, probably due to the stress generated by the infiltration. However, SA induced increases in PA content from 24 h till 96 hai, with a maximum of 6.2-fold

24h 48h 72h 96h

**Table 1.** Variations in PA levels in SA-infiltrated wheat leaves compared to the water-infiltrated control

These results confirm some variations in PA content reported by several authors. Treatment of *A. thaliana* protoplasts with H2O2 increases PA content by 30% [52]. Furthermore, elicitors from plant pathogens activate the PLC-DGK pathway, which consisted of a rapid accumulation of PA within 2 minutes in transgenic tobacco cells treated with the race-specific elicitor Avr4 [53]. A transient accumulation of PA was also recorded in suspension-cultured tomato cells treated with the general elicitors N,N',N'',N'''-tetraacetyl-chitotetraose, xylanase, and the flagellin-derived peptide flg22 [54]. In rice cells, the PA amount increased rapidly after treatment with Nacetylchitooligosaccharide elicitor [55]. Moreover, the PA increase is likely to occur upstream of the oxidative burst [53,55]. Furthermore, method of PA assessment. Furthermore, all these studies point out the rapid accumulation of PA upon treatments, generally within minutes. According to [16], signaling lipids, unlike structural lipids, are present only in minute amounts, yet their levels increase rapidly in response to certain stimuli. Such an accumulation is transient because the signal is rapidly down regulated. However, none of these characteristics, namely the rapid and transient accumulation upon treatment, met our results. SA induces a PA accumulation that occurred not earlier than 24 h after SA infiltration and seemed to last for at least 4 days. This result, that does not match the general trend, may be explained by a late induction of one or both of the phospholipases pathways leading to PA formation. Since the magnitude of PA change varies upon the treatment, tissue and method of PA assessment [17], our findings could be attributed to the treatment and/or to the tissue nature - infiltration of SA and PA assessment *in planta* - whereas most of the studies are conducted on cellular cultures.

Time after SA infiltration

increase 2.7-fold increase 6.2-fold increase 1.19-fold increase

In order to corroborate the PA formation with the PLC-DGK pathway activation, the expression of the *PLC* gene, encoding a phospholipase C, was measured over the timecourse experiment, compared to the water-infiltrated wheat leaves, and normalized to two reference genes, *tub* and *ef1α*, encoding tubulin and elongation factor, respectively (Figure 5). The expression pattern of the *PLC* gene consisted of three up-regulations: 3.5 and 4.8-fold increases were induced at 9 and 21 hai, respectively. Furthermore, this gene expression was strongly increased from 48 till 96 hai, with an average of 9-fold increase over this period. This late high up-regulation of *PLC* gene correlates with the late PA detection in wheat leaves between 48 and 96 h after SA infiltration. The accumulation of PA is probably due to this pathway's stimulation after *PLC* gene's expression and synthesis of the corresponding enzyme.

**Figure 5.** *PLC* gene expression in wheat leaves infiltrated with SA

PA formation through the phospholipase C pathway results from two enzymes acting sequentially: PLC hydrolyses phosphatidylinositol-4,5-bisphosphate [PtdIns(4,5)P2, also abbreviated as PIP2] into inositol-1,4,5-trisphosphate [Ins(1,4,5)P3] and DAG. DAG remains in the membrane and is rapidly phosphorylated to PA by DGK (Figure 1). The variation in DAG levels in SA-infiltrated wheat leaves is presented in table 2. During the first 24 h after SA infiltration, no clear variation pattern in DAG content was observed. However, SA induced the accumulation of DAG from 24 till 96 hai, with a maximum of 2.18-fold increase at 72hai. Interestingly, DAG accumulation, as well as *PLC* gene expression, was recorded in the same period of the time-course experiment, 24 till 96 h after SA infiltration. The DAG accumulation seems to be the consequence of the induction of *PLC* gene expression.

Twenty four hours after infiltration, SA induces the expression of PLC-encoding gene, simultaneously with an accumulation of DAG and PA. One could think that DAG content must decrease in order to fulfill PA formation; indeed, the contribution of DAG could only

#### 372 Lipid Metabolism

be confirmed by the investigation of DGK activity. Even if the subsequent enzymatic conversion of DAG doesn't lead to PA formation, one must keep in mind that the hydrolysis of PtdIns(4,5)P2 into Ins(1,4,5)P3 is of a great interest since the latter diffuses into the cytosol where it possibly triggers calcium flux/release from intracellular stores [20].

Lipids as Markers of Induced Resistance in Wheat: A Biochemical and Molecular Approach 373

responses mediated by ABA without any contribution of DGK activity or PA [57]. This signaling, *via* Ins(1,4,5)P3, is also reported as an early response to salinity and hyperosmotic stress [58,59]. The PLC-DGK pathway was sought in *Arabidopsis* after cold exposure [60], in transgenic tobacco cells upstream the oxidative burst as in [53] and after contact with pathogens. In suspension-cultured alfalfa cells, the nod factor activates this pathway [61].

Treatment of tomato cell cultures with the fungal elicitor xylanase resulted in a rapid and dose-dependent nitric oxide (NO) accumulation, required for PA production via the activation of PLC-DGK pathway. PA and, correspondingly, xylanase were shown to induce

The PLD pathway is involved in every mentioned stress signaling, except cold-induced stress. Several *Arabidopsis* PLDs were found to be induced in response to *Pseudomonas* infection [63]. The PLD pathway contribution was also found in *Arabidopsis* upon drought

Moreover, signaling lipids can affect the activity of target enzymes. In [69], the authors showed an activation of a calcium-dependent protein kinase DcCPK1 by PA in *Daucus carota*. In *Arabidopsis,* the activation of AtPDK1, a protein kinase, target of PLD-generated PA, is involved in root hair growth [70]; the PLD-derived PA also interacts with ABI1

All together, these results are the first evidence for SA as an inducer of PA formation in wheat leaves. Increases in PA levels in SA-treated wheat leaves seem to be highly related to the induction of plant genes encoding phospholipases that are involved in the synthesis or

**3.4.** *LOX* **gene expression and LOX activity are enhanced upon SA-infiltration** 

In the present experiments, the *lox* gene expression showed a 12 and 14-fold increase at 9 and 21 hai respectively, in SA-infiltrated leaves. This gene expression was also strongly induced later, with a 166 and 156-fold increase at 48 and 96 hai respectively (Figure 6).

In grapevine plantlets, rhamnolipids induced for *lox* gene expression a 7-fold increase 24 h after immersion in the rhamnolipids solution [72]. In wheat, transcripts of *WCI-2* (Wheat Chemically Induced gene) gene, which encodes a lipoxygenase, accumulated quickly in response to MeJA, SA and BTH treatments (from 2 h to 24h for MeJA, and from 4h and to 20 h for the other elicitors); however, SA induced this gene's expression to a lesser extent than the other two compounds [73]. The contribution of SA to early signaling events by the stimulation of lipoxygenase-encoding genes is therefore established. Nevertheless, the authors didn't record any accumulation of the transcripts of *WCI-2* gene the first 24 h after wheat seedlings inoculation with *Bgt* nor *Bgh* (incompatible interaction). However, accumulation of these transcripts was found in latter stages of wheat infection with powdery mildew. In infectious conditions, the *lox* gene seemed to be expressed quite late [74]. Infiltration with SA reproduced a similar lox-encoding transcripts profile with a late up-regulation of the *lox* gene to a 166 and 156-fold increase at 48 and 96 hai, respectively.

[64], ethylene treatment [65], freezing [66] and wounding [67,68].

phosphatase and regulates ABA signaling [71].

ROS production [62].

release of PA.

In addition, the simultaneous increase of these compounds could be due to the durable *PLC* gene expression, ensuring a continuous supply of DAG to be phosphorylated to PA.


**Table 2.** Variation in DAG level in SA-infiltrated wheat leaves compared to the control

#### **3.3. PE and PC contents vary in SA-infiltrated leaves**

PA could also be generated by the phospholipase D pathway which hydrolyzes structural membrane phospholipids such as PE and PC (Figure 1). The variations of PE and PC levels in SA-infiltrated leaves compared to the control are presented in table 3. While accumulation of PC was observed during the whole time-course experiment (except for 24 and 96 hai), PE accumulated the first 18h after treatment. Afterward, SA induced a decrease in the PE content between 24 and 96 hai, with a maximum decrease at 48 and 72 hai. These results match the increased PA level in SA-infiltrated wheat leaves in the same period, suggesting that this pathway is involved in PA formation. Since PC level was maintained and even increased, this phospholipid doesn't seem to be involved in PA production, under SA treatment. The PE/PC ratio is also reduced from 48 till 96 hai. Substantial alterations in the lipid composition of plasma membrane are a widely known process to stress adaptation, such as water deficit: the PC/PE ratio changed from 1.1 in plants non-acclimated to water stress to 0.69 in acclimated ones [56].


**Table 3.** Variations in PE and PC levels (compared to the control) and PE/PC ratio induced in SAinfiltrated wheat leaves

In conclusion, SA seems to induce the formation of PA through the activation of phospholipases C and/or D pathways. In *Arabidopsis*, PLC signaling is involved in some responses mediated by ABA without any contribution of DGK activity or PA [57]. This signaling, *via* Ins(1,4,5)P3, is also reported as an early response to salinity and hyperosmotic stress [58,59]. The PLC-DGK pathway was sought in *Arabidopsis* after cold exposure [60], in transgenic tobacco cells upstream the oxidative burst as in [53] and after contact with pathogens. In suspension-cultured alfalfa cells, the nod factor activates this pathway [61].

372 Lipid Metabolism

PE content

PC content

1.4-fold increase

1.4-fold increase

infiltrated wheat leaves

1.3-fold

2.9-fold increase

DAG content 1.1-fold

increase

be confirmed by the investigation of DGK activity. Even if the subsequent enzymatic conversion of DAG doesn't lead to PA formation, one must keep in mind that the hydrolysis of PtdIns(4,5)P2 into Ins(1,4,5)P3 is of a great interest since the latter diffuses into the cytosol

In addition, the simultaneous increase of these compounds could be due to the durable *PLC*

6h 12h 18h 24h 48h 72h 96h

PA could also be generated by the phospholipase D pathway which hydrolyzes structural membrane phospholipids such as PE and PC (Figure 1). The variations of PE and PC levels in SA-infiltrated leaves compared to the control are presented in table 3. While accumulation of PC was observed during the whole time-course experiment (except for 24 and 96 hai), PE accumulated the first 18h after treatment. Afterward, SA induced a decrease in the PE content between 24 and 96 hai, with a maximum decrease at 48 and 72 hai. These results match the increased PA level in SA-infiltrated wheat leaves in the same period, suggesting that this pathway is involved in PA formation. Since PC level was maintained and even increased, this phospholipid doesn't seem to be involved in PA production, under SA treatment. The PE/PC ratio is also reduced from 48 till 96 hai. Substantial alterations in the lipid composition of plasma membrane are a widely known process to stress adaptation, such as water deficit: the PC/PE ratio changed from 1.1 in plants non-acclimated to water stress to 0.69 in acclimated ones [56].

Time after SA infiltration

decrease

increase Ø 1.8-fold

6.6-fold decrease

increase

6.2-fold decrease

1.4-fold

increase <sup>Ø</sup>

2.3-fold decrease

6h 12h 18h 24h 48h 72h 96h

PE/PC 2 1.2 1.2 2.2 0.2 0.5 0.6

In conclusion, SA seems to induce the formation of PA through the activation of phospholipases C and/or D pathways. In *Arabidopsis*, PLC signaling is involved in some

**Table 3.** Variations in PE and PC levels (compared to the control) and PE/PC ratio induced in SA-

increase Ø 1.3-fold

3.8-fold

increase

Time after SA infiltration

1.62-fold increase

2.18-fold increase

1.56-fold increase

gene expression, ensuring a continuous supply of DAG to be phosphorylated to PA.

decrease Ø 1.26-fold

**Table 2.** Variation in DAG level in SA-infiltrated wheat leaves compared to the control

where it possibly triggers calcium flux/release from intracellular stores [20].

1.1-fold

**3.3. PE and PC contents vary in SA-infiltrated leaves** 

Treatment of tomato cell cultures with the fungal elicitor xylanase resulted in a rapid and dose-dependent nitric oxide (NO) accumulation, required for PA production via the activation of PLC-DGK pathway. PA and, correspondingly, xylanase were shown to induce ROS production [62].

The PLD pathway is involved in every mentioned stress signaling, except cold-induced stress. Several *Arabidopsis* PLDs were found to be induced in response to *Pseudomonas* infection [63]. The PLD pathway contribution was also found in *Arabidopsis* upon drought [64], ethylene treatment [65], freezing [66] and wounding [67,68].

Moreover, signaling lipids can affect the activity of target enzymes. In [69], the authors showed an activation of a calcium-dependent protein kinase DcCPK1 by PA in *Daucus carota*. In *Arabidopsis,* the activation of AtPDK1, a protein kinase, target of PLD-generated PA, is involved in root hair growth [70]; the PLD-derived PA also interacts with ABI1 phosphatase and regulates ABA signaling [71].

All together, these results are the first evidence for SA as an inducer of PA formation in wheat leaves. Increases in PA levels in SA-treated wheat leaves seem to be highly related to the induction of plant genes encoding phospholipases that are involved in the synthesis or release of PA.

#### **3.4.** *LOX* **gene expression and LOX activity are enhanced upon SA-infiltration**

In the present experiments, the *lox* gene expression showed a 12 and 14-fold increase at 9 and 21 hai respectively, in SA-infiltrated leaves. This gene expression was also strongly induced later, with a 166 and 156-fold increase at 48 and 96 hai respectively (Figure 6).

In grapevine plantlets, rhamnolipids induced for *lox* gene expression a 7-fold increase 24 h after immersion in the rhamnolipids solution [72]. In wheat, transcripts of *WCI-2* (Wheat Chemically Induced gene) gene, which encodes a lipoxygenase, accumulated quickly in response to MeJA, SA and BTH treatments (from 2 h to 24h for MeJA, and from 4h and to 20 h for the other elicitors); however, SA induced this gene's expression to a lesser extent than the other two compounds [73]. The contribution of SA to early signaling events by the stimulation of lipoxygenase-encoding genes is therefore established. Nevertheless, the authors didn't record any accumulation of the transcripts of *WCI-2* gene the first 24 h after wheat seedlings inoculation with *Bgt* nor *Bgh* (incompatible interaction). However, accumulation of these transcripts was found in latter stages of wheat infection with powdery mildew. In infectious conditions, the *lox* gene seemed to be expressed quite late [74]. Infiltration with SA reproduced a similar lox-encoding transcripts profile with a late up-regulation of the *lox* gene to a 166 and 156-fold increase at 48 and 96 hai, respectively.

Lipids as Markers of Induced Resistance in Wheat: A Biochemical and Molecular Approach 375

In non-infectious context, the induction of a LOX activity was also assessed in wheat by Renard-Merlier *et al*. [8]. Wheat sprayings with HSA enhanced a 1.5-fold increase in LOX activity, compared to corresponding ethanol control, only 96 h after treatment. Thus, infiltration of SA as well as HSA sprayings induced similar LOX enzymatic activity profile. However, these authors didn't report any significant difference in LOX activity between control and SA-sprayed leaves over the 4 days after treatment. This finding highlights the effect of SA functionalization, probably improving the penetration of HSA through the hydrophobic plant cuticle. Moreover, HSA, which increased the protection level against *Bgt* from 50% in SA-treated wheat leaves to 95%, induced an 8-fold increase of the LOX activity

LOX-derived products such as hydroperoxy, hydroxyl and keto fatty acids accumulate in plants in response to attack by pathogens and treatment with inducers of plant defence responses [75]. For example, in *A. thaliana*, infection by *P. syringae* causes accumulation of ketodienoic fatty acids in *A.* leaves as well as the cell death and induces expression of the *GST1* gene, which encodes a glutathione-S-transferase [76]. In another study, SA treatment was shown to cause the accumulation of 13 (S)-hydroxyoctadecatrienoic acid (13-HOTrE) in barley leaves, and application of 13-HOTrE induces the expression of the PR1B gene, suggesting the involvement of 13-HOTrE in SA signaling in barley [77]. One must keep in mind that the primary products of PUFAs enzymatic oxidation are often converted to oxylipins such as JA. In barley leaves, 13-HOD and 13-HOT (hydroxyl PUFAs after reductase on HPOD and HPOT respectively) accumulated suggesting that the reductase branch of the LOX pathway is the object of preferential induction upon SA treatment, among the various metabolic transformations of the LOX-derived 13-HPOT or 13-HPOD. No accumulation of other LOX pathway-products was observed. SA as well as 13-HOT induced *PR1* gene expression, 48h after treatment. In barley leaves, at least one specific LOX is transcriptionnaly activated by SA and JA. This LOX-100 is a 13-LOX located in the chloroplast. However, this LOX-100 gene was not expressed upon infection with powdery mildew in susceptible and non-susceptible barley lines [78]. The co-induction of LOX and

in inoculated conditions.

infection.

presented above.

PR1 by SA suggests a role in plant defense reaction.

**3.5. FAs content varies in resistance inducers-treated wheat plants** 

*3.5.1. Total FAs content vary in trehalose, Iodus40, Milsana and HSA-treated wheat leaves* 

In wheat, Renard-Merlier *et al*. [46] conducted a global investigation of total FA content in relation to treatment with four inducers of resistance and to powdery mildew

Table 4 presents a summary of the observed variations of several FAs content at the quantitative and qualitative levels induced by the four tested resistance inducers and these results are now discussed on the basis of the most recent literature as well as our results

**Figure 6.** *lox* gene expression in wheat leaves treated with SA

Figure 7 shows the LOX activity in leaf extracts at 6, 12, 18, 24, 48, 72 and 96 h after SA infiltration in comparison to water-infiltrated leaves. During the first 48h, the LOX activity was decreased in SA-infiltrated leaves. However, SA induced significant 1.7 and 3.8-fold increases in LOX activity at 72h and 96hai, compared to the control.

**Figure 7.** Time-course activity of LOX in water and SA-infiltrated wheat leaves. Data represent means of 3 independent experiments. Bars with an asterix are different from water control plantlets as determined by ANOVA followed by a multiple range test (LSD) (P<0.05).

When compared together, profiles of *lox* gene expression and LOX activity in SA-infiltrated leaves show interestingly that the first *lox* up-regulations, 24 h after SA infiltration, are not followed by the corresponding enzymatic activity. Induction of LOX activity by SA was only detectable after *lox* transcripts accumulation was the most important, between 48 and 96hai.

In non-infectious context, the induction of a LOX activity was also assessed in wheat by Renard-Merlier *et al*. [8]. Wheat sprayings with HSA enhanced a 1.5-fold increase in LOX activity, compared to corresponding ethanol control, only 96 h after treatment. Thus, infiltration of SA as well as HSA sprayings induced similar LOX enzymatic activity profile. However, these authors didn't report any significant difference in LOX activity between control and SA-sprayed leaves over the 4 days after treatment. This finding highlights the effect of SA functionalization, probably improving the penetration of HSA through the hydrophobic plant cuticle. Moreover, HSA, which increased the protection level against *Bgt* from 50% in SA-treated wheat leaves to 95%, induced an 8-fold increase of the LOX activity in inoculated conditions.

374 Lipid Metabolism

**Figure 6.** *lox* gene expression in wheat leaves treated with SA

increases in LOX activity at 72h and 96hai, compared to the control.

determined by ANOVA followed by a multiple range test (LSD) (P<0.05).

Figure 7 shows the LOX activity in leaf extracts at 6, 12, 18, 24, 48, 72 and 96 h after SA infiltration in comparison to water-infiltrated leaves. During the first 48h, the LOX activity was decreased in SA-infiltrated leaves. However, SA induced significant 1.7 and 3.8-fold

**Figure 7.** Time-course activity of LOX in water and SA-infiltrated wheat leaves. Data represent means of 3 independent experiments. Bars with an asterix are different from water control plantlets as

When compared together, profiles of *lox* gene expression and LOX activity in SA-infiltrated leaves show interestingly that the first *lox* up-regulations, 24 h after SA infiltration, are not followed by the corresponding enzymatic activity. Induction of LOX activity by SA was only detectable after *lox* transcripts accumulation was the most important, between 48 and 96hai.

LOX-derived products such as hydroperoxy, hydroxyl and keto fatty acids accumulate in plants in response to attack by pathogens and treatment with inducers of plant defence responses [75]. For example, in *A. thaliana*, infection by *P. syringae* causes accumulation of ketodienoic fatty acids in *A.* leaves as well as the cell death and induces expression of the *GST1* gene, which encodes a glutathione-S-transferase [76]. In another study, SA treatment was shown to cause the accumulation of 13 (S)-hydroxyoctadecatrienoic acid (13-HOTrE) in barley leaves, and application of 13-HOTrE induces the expression of the PR1B gene, suggesting the involvement of 13-HOTrE in SA signaling in barley [77]. One must keep in mind that the primary products of PUFAs enzymatic oxidation are often converted to oxylipins such as JA. In barley leaves, 13-HOD and 13-HOT (hydroxyl PUFAs after reductase on HPOD and HPOT respectively) accumulated suggesting that the reductase branch of the LOX pathway is the object of preferential induction upon SA treatment, among the various metabolic transformations of the LOX-derived 13-HPOT or 13-HPOD. No accumulation of other LOX pathway-products was observed. SA as well as 13-HOT induced *PR1* gene expression, 48h after treatment. In barley leaves, at least one specific LOX is transcriptionnaly activated by SA and JA. This LOX-100 is a 13-LOX located in the chloroplast. However, this LOX-100 gene was not expressed upon infection with powdery mildew in susceptible and non-susceptible barley lines [78]. The co-induction of LOX and PR1 by SA suggests a role in plant defense reaction.

#### **3.5. FAs content varies in resistance inducers-treated wheat plants**

#### *3.5.1. Total FAs content vary in trehalose, Iodus40, Milsana and HSA-treated wheat leaves*

In wheat, Renard-Merlier *et al*. [46] conducted a global investigation of total FA content in relation to treatment with four inducers of resistance and to powdery mildew infection.

Table 4 presents a summary of the observed variations of several FAs content at the quantitative and qualitative levels induced by the four tested resistance inducers and these results are now discussed on the basis of the most recent literature as well as our results presented above.

#### 376 Lipid Metabolism


Lipids as Markers of Induced Resistance in Wheat: A Biochemical and Molecular Approach 377

enhanced resistance to the aphid *Myzus persicae*, the fungal pathogen *Botrytis cinerea* and to

C18:1 (oleic acid) in Iodus 40®-treated (ni) plants showed a quantitative 1.2 fold-increase. C18:1, as well as other C18 and C16 FAs, are well known substrates for cutin monomer synthesis [83]. One could suggest that Iodus 40®, by stimulating the accumulation of this FA, contributes to the reinforcement of the plant cuticule prior to fungal contamination. In cultured parsley cells, a biphasic time-course for C18:1 increase was obtained upon treatment with peptidic or fungal elicitors [84]. In [85], the authors suggested that chloroplastic C18:1 level is critical for normal pathogen defense responses in *Arabidopsis,* including programmed cell death and systemic acquired resistance (SAR). In [86], it was shown that the oleic acid-mediated pathway induces constitutive defense signaling and enhances resistance to multiple pathogens in soybean. C18:1 and linoleic (18:2) acid levels, in part, regulate fungal development, seed colonization, and mycotoxin production by *Aspergillus* spp. [87]. Direct antifungal activity has also been reported for C18:1, since it inhibits, in a dose-dependent manner, the germination of *Erysiphe polygoni* spores [88].

The amount of C18:2 increased (1.6-fold) 4 days after HSA treatment in (i) plants. For C18:2, the accumulation in sorbitol-treated barley leaves was reported from 12 h till 72h after treatment [89]. Cold acclimating potato was found to accumulate linoleic acid (18:2) in the membrane glycerolipids of the leaves [90]. C18:2 is also a substrate for cutin monomer

Among the four inducers tested, Iodus40® had the largest effects on FA levels, since it increased C12:0 and C18:1 and decreased C20:2. This product, which active ingredient is laminarin (polysaccharide), induced decreases in lipid peroxydation level all over the time-

Trehalose and Milsana® had similar effects on FAs profile with induced increases in C12:0 and decreases in C20:2 contents. However, TR and Milsana® modes of action are quite different in the wheat-powdery mildew interaction. TR activates phenylalanine ammonialyase (PAL) and peroxydase activity and enhances papilla autofluorescence and H2O2 accumulation. However, it does not affect catalase (CAT), cinnamyl alcohol dehydrogenase (CAD), LOX or oxalate oxidase (OXO) activities, and does not alter lipid peroxide levels [8]. According to the authors in [10], treatments of wheat with Milsana® enhance H2O2 accumulation at the fungal penetration site without any possible correlation with the activation of enzymes involved in ROS metabolism. Only LOX, involved in both ROS regulation and lipid peroxidation, showed a 26 to 32% increase 48h postreatment in Milsana-infiltrated leaves. This weak effect of Milsana® on wheat lipid metabolism was confirmed at the lipid peroxydation level, which was shown to decrease in treated plants.

While HSA sprayings enhanced an increase in C18:2 levels only, HSA exhibited the most numerous and the highest effects in the wheat-powdery mildew interaction. HSA induced H2O2 accumulation, increases LOX activity in (i) conditions and decreases CAT activity in

synthesis and can therefore contribute to cuticle reinforcement.

course experiment [8].

(ni) context [8].

the oomycete pathogen *Phytophtora capsici*.

**Table 4.** Summary of variations observed in C12:0, C18:1, C18:2 and C20:2 content at the quantitative (μg.mg -1 dry weight) and qualitative (percentage of total FAs) levels induced by inoculation, trehalose, Iodus 40®, Milsana® or HSA sprayings. These variations are observed 4 days after sprayings in non inoculated (ni) plants and 2 days post inoculation in inoculated (i) conditions

Lauric acid (C12:0) content quantitatively increased after Iodus 40® (2.8-fold), Milsana® (4.8-fold) and trehalose (4-fold) treatment in (i) plants (2 days after inoculation). In [79], the authors showed that *Vicia sativa* seedlings treated with MeJA exhibit an increase in lauric acid ω-hydroxylase activity, an enzyme that converts C12:0 into hydroxylated forms potentially involved in cutin monomer synthesis. Moreover, C12:0 itself has several relevant biological properties such as antifungal, antiviral, antiparasite and antibacterial activities [80,81]. However, none of the four compounds induced any variation in C12:0 level in noninfectious conditions. Since no elicitation was observed in this context, priming effect on C12:0 accumulations could be proposed for these resistance inducers in wheat against *Bgt.*

Contents of C20:2 (eicosadienoic acid) decreased in Iodus 40®- and Milsana®-treated (ni) plants compared to the corresponding controls (4 days after treatment). The decrease was confirmed at the qualitative level only for Iodus 40®. In (i) conditions, only Milsana® induced a significant decrease in C20:2 content at both levels whereas TR induced a decrease perceptible at the qualitative level only. In (i) plants, C20:2 increased (data not shown). C20:2 content seemed to be affected by fungal infection of the plant to a greater extent than by any of the resistance inducing treatments, since similar quantities were found in watercontrol (i) plants as well as in resistance inducers-treated plants. The link between C20:2 and infection was also reported in [82].Transgenic *A. thaliana* plants producing C20:2 exhibited enhanced resistance to the aphid *Myzus persicae*, the fungal pathogen *Botrytis cinerea* and to the oomycete pathogen *Phytophtora capsici*.

376 Lipid Metabolism

Trehalose

Iodus 40

Milsana

HSA

<sup>i</sup>4.0-fold

<sup>i</sup>2.8-fold

<sup>i</sup>4.8-fold

increase

increase

increase

ni Ø Ø 1.2-fold

2.4-fold

1.5-fold

1.5-fold

 C12:0 C18:1 18:2 C20:2 quantitative qualitative quantitative qualitative quantitative qualitative quantitative qualitative

ni Ø Ø Ø Ø Ø Ø Ø Ø

2.2-fold

**Table 4.** Summary of variations observed in C12:0, C18:1, C18:2 and C20:2 content at the quantitative (μg.mg -1 dry weight) and qualitative (percentage of total FAs) levels induced by inoculation, trehalose, Iodus 40®, Milsana® or HSA sprayings. These variations are observed 4 days after sprayings in non

Lauric acid (C12:0) content quantitatively increased after Iodus 40® (2.8-fold), Milsana® (4.8-fold) and trehalose (4-fold) treatment in (i) plants (2 days after inoculation). In [79], the authors showed that *Vicia sativa* seedlings treated with MeJA exhibit an increase in lauric acid ω-hydroxylase activity, an enzyme that converts C12:0 into hydroxylated forms potentially involved in cutin monomer synthesis. Moreover, C12:0 itself has several relevant biological properties such as antifungal, antiviral, antiparasite and antibacterial activities [80,81]. However, none of the four compounds induced any variation in C12:0 level in noninfectious conditions. Since no elicitation was observed in this context, priming effect on C12:0 accumulations could be proposed for these resistance inducers in wheat against *Bgt.*

Contents of C20:2 (eicosadienoic acid) decreased in Iodus 40®- and Milsana®-treated (ni) plants compared to the corresponding controls (4 days after treatment). The decrease was confirmed at the qualitative level only for Iodus 40®. In (i) conditions, only Milsana® induced a significant decrease in C20:2 content at both levels whereas TR induced a decrease perceptible at the qualitative level only. In (i) plants, C20:2 increased (data not shown). C20:2 content seemed to be affected by fungal infection of the plant to a greater extent than by any of the resistance inducing treatments, since similar quantities were found in watercontrol (i) plants as well as in resistance inducers-treated plants. The link between C20:2 and infection was also reported in [82].Transgenic *A. thaliana* plants producing C20:2 exhibited

increase

ni Ø Ø Ø Ø Ø 1.15-fold

inoculated (ni) plants and 2 days post inoculation in inoculated (i) conditions

i Ø Ø Ø Ø 1.6-fold

ni Ø Ø Ø Ø Ø Ø 2.3-fold

increase Ø Ø Ø Ø Ø 1.3-fold

increase Ø Ø Ø Ø Ø Ø

increase

increase Ø Ø Ø Ø 1.8-fold

increase Ø Ø 1.5-fold

decrease

1.33-fold decrease

2.0-fold decrease

decrease

decrease

increase Ø Ø

increase Ø Ø

1.15-fold

decrease <sup>Ø</sup>

C18:1 (oleic acid) in Iodus 40®-treated (ni) plants showed a quantitative 1.2 fold-increase. C18:1, as well as other C18 and C16 FAs, are well known substrates for cutin monomer synthesis [83]. One could suggest that Iodus 40®, by stimulating the accumulation of this FA, contributes to the reinforcement of the plant cuticule prior to fungal contamination. In cultured parsley cells, a biphasic time-course for C18:1 increase was obtained upon treatment with peptidic or fungal elicitors [84]. In [85], the authors suggested that chloroplastic C18:1 level is critical for normal pathogen defense responses in *Arabidopsis,* including programmed cell death and systemic acquired resistance (SAR). In [86], it was shown that the oleic acid-mediated pathway induces constitutive defense signaling and enhances resistance to multiple pathogens in soybean. C18:1 and linoleic (18:2) acid levels, in part, regulate fungal development, seed colonization, and mycotoxin production by *Aspergillus* spp. [87]. Direct antifungal activity has also been reported for C18:1, since it inhibits, in a dose-dependent manner, the germination of *Erysiphe polygoni* spores [88].

The amount of C18:2 increased (1.6-fold) 4 days after HSA treatment in (i) plants. For C18:2, the accumulation in sorbitol-treated barley leaves was reported from 12 h till 72h after treatment [89]. Cold acclimating potato was found to accumulate linoleic acid (18:2) in the membrane glycerolipids of the leaves [90]. C18:2 is also a substrate for cutin monomer synthesis and can therefore contribute to cuticle reinforcement.

Among the four inducers tested, Iodus40® had the largest effects on FA levels, since it increased C12:0 and C18:1 and decreased C20:2. This product, which active ingredient is laminarin (polysaccharide), induced decreases in lipid peroxydation level all over the timecourse experiment [8].

Trehalose and Milsana® had similar effects on FAs profile with induced increases in C12:0 and decreases in C20:2 contents. However, TR and Milsana® modes of action are quite different in the wheat-powdery mildew interaction. TR activates phenylalanine ammonialyase (PAL) and peroxydase activity and enhances papilla autofluorescence and H2O2 accumulation. However, it does not affect catalase (CAT), cinnamyl alcohol dehydrogenase (CAD), LOX or oxalate oxidase (OXO) activities, and does not alter lipid peroxide levels [8]. According to the authors in [10], treatments of wheat with Milsana® enhance H2O2 accumulation at the fungal penetration site without any possible correlation with the activation of enzymes involved in ROS metabolism. Only LOX, involved in both ROS regulation and lipid peroxidation, showed a 26 to 32% increase 48h postreatment in Milsana-infiltrated leaves. This weak effect of Milsana® on wheat lipid metabolism was confirmed at the lipid peroxydation level, which was shown to decrease in treated plants.

While HSA sprayings enhanced an increase in C18:2 levels only, HSA exhibited the most numerous and the highest effects in the wheat-powdery mildew interaction. HSA induced H2O2 accumulation, increases LOX activity in (i) conditions and decreases CAT activity in (ni) context [8].

#### 378 Lipid Metabolism

While barley leaves treated with salicylate [77], sorbitol [89] or JA [91] accumulated C18:3, none of the 4 compounds tested induced any increase in C18:3 in wheat leaves according to our results.

Lipids as Markers of Induced Resistance in Wheat: A Biochemical and Molecular Approach 379

A 2,7-fold decrease in C18:2 PLFAs was observed 72 till 96 hai of SA. In sorbitol-treated barley leaves, the accumulation of C18:2 occurred from 12 h till 72h after treatment [89]. The development of asexual spores, and the formation of cleistothecia and sclerotia of *Aspergillus* spp are affected by C18:2 and light [95]. Avocado fruits infected with *Colletotrichum*

One of the most interesting results is the general decrease of C18:3 level after SA-infiltration. Most of the studies report increases in 18:3 levels such in suspension cells of California poppy (*Eschscholtzia californica*) treated with a yeast elicitor [97]. In *A. thaliana,* an increase of C18:3 occurred within a few hours of exposure to an avirulent strain of *P. syringae* [91]. The *Arabidopsis fad7 fad8* mutant defective in the generation of C18:3 in chloroplastic membranes is deficient in ROS production following infection with avirulent strains of *Pseudomonas syringae* and shows enhanced susceptibility to this pathogen [92]. C18:3 stimulates NADPH oxidase activity *in vitro,* which suggests that C18:3 modulates ROS production and the subsequent defense responses during *R* gene–mediated resistance in plants [92]. The *Arabidopsis fad3 fad7 fad8* triple mutant is unable to accumulate JA because of a deficiency in C18:3 and is highly susceptible to infection by insect larvae [98]. The *fad3 fad7 fad8* mutant plants are also highly susceptible to root rot by *Pythium jasmonium*, and this susceptibility can be alleviated by the exogenous application of MeJA [99]. Rhizobacteria-induced enhanced resistance to *Botrytis cinerea* is associated with the accumulation of C18:2 and

In barley leaves, 13-LOX are induced by SA and jasmonates. Upon SA treatment, free C18:3 and C18:2 accumulate in a 10:1 ratio reflecting their relative occurrence in leaf tissues [78]. The release of 18:3 from plant membrane lipids by stress-activated lipases is thought to provide the substrate for lipoxygenase and subsequent octadecanoid (oxylipin) pathway synthesis of JA and methyl jasmonate [101,102]. JA and methyl jasmonate participate in the signal regulation of a number of plant processes including wound and pathogen defense responses. Efforts have been successful to identify and characterize fatty acids esterifying lipases that are activated by pathogen attack and/or environmental stress. Results suggest that both A1 and A2 phospholipases are involved in 18:3 mobilization form membrane lipids [103]. In the C4 monocotyledon sorghum (*Sorghum bicolor L.),* SA induced genes of the octadecanoic acid pathway for JA synthesis which

However, in tobacco tissues expressing a hypersensitive response to TMV, an increase in the saturation of fatty acids contained in the microsomal phospholipids was observed while C18:3 content decreased by 9% [105]. Interestingly, the authors credited the change of FAs

The decreases in free FAs observed with our model could be explained by a rapid dioxygenation *via* LOX activity. Furthermore, accumulation of C16:0 and C18:0 coupled with no significant increase in C18:1 means that elongation of C16:0 into C18:0 is not followed by desaturation into C18:1, C18:2 and finally, C18:3. Such results could explain the

composition to a four-fold increase in LOX activity of the infected tobacco tissues.

*gleosporioides* spores accumulate C18:2 [96].

C18:3 FAs in bean [100].

resulted in higher JA content [104].

reduced content level of C18:3.

#### *3.5.2. Free FAs and PLFAs content vary in SA-infiltrated wheat leaves*

The profile of free FAs and phospholipids FAs (PLFAs) in SA-infiltrated wheat leaves were also investigated and are presented in Table 5 and Table 6.



**Table 5.** Variations in free FAs content and % in SA-infiltrated leaves

**Table 6.** Variations in PLFAs content and % in SA-infiltrated leaves

Upon treatment with SA, free palmitic acid (C16:0) accumulation was observed from 48 till 96 hai with an average of 2.38 fold-increase over this period and 1.4-fold increase at the qualitative level over the whole time-course experiment. Similar results were observed for the PLFAs C16:0, essentially the last 3 days of the experiment. Since monomers of cutin are synthesized C16:0, SA seems to induce the reinforcement of the plant cuticule. In *A. thaliana*, levels of the C16:3 (hexadecatrienoic acid) increase within a few hours of exposure to an avirulent strain of *P. syringae* [92].

Increases in both classes of stearic acid C18:0 content and percentage were observed in SAinfiltrated leaves. In soybean, increased levels of C18:0 likely inhibit soybean seed colonization by the seed-borne pathogen *Diaporthe phaseolorum* [93].

A transient 2-fold increase in free FAs C18:1 content was recorded. A sharp and rapid increase in C18:1 level was observed in parsley cells treated with a fungal elicitor [83]. Recent studies suggest that free oleic acid (18:1) levels in the chloroplast regulate the defense response of plants to pathogens including programmed cell death and SAR [94].

A 2,7-fold decrease in C18:2 PLFAs was observed 72 till 96 hai of SA. In sorbitol-treated barley leaves, the accumulation of C18:2 occurred from 12 h till 72h after treatment [89]. The development of asexual spores, and the formation of cleistothecia and sclerotia of *Aspergillus* spp are affected by C18:2 and light [95]. Avocado fruits infected with *Colletotrichum gleosporioides* spores accumulate C18:2 [96].

378 Lipid Metabolism

our results.

μg/mg dry weight

μg/mg dry weight

%

%

While barley leaves treated with salicylate [77], sorbitol [89] or JA [91] accumulated C18:3, none of the 4 compounds tested induced any increase in C18:3 in wheat leaves according to

The profile of free FAs and phospholipids FAs (PLFAs) in SA-infiltrated wheat leaves were

C16:0 C18:0 C18:1 C18:2 C18:3

(6-96hai) Ø Ø

Ø

Ø Ø

2-fold increase (48-96hai)

Ø

2.7-fold decrease (72-96hai) 2.74-fold decrease (6-96hai)

2.3-fold decrease (6-96hai)

> 2.28-fold decrease (24-96hai)

> 1.27-fold decrease (24-96hai)

*3.5.2. Free FAs and PLFAs content vary in SA-infiltrated wheat leaves* 

2.36-fold increase (48-96hai)

1.47-fold increase

1.9-fold increase (6-96hai)

2.5-fold increase (24-96hai)

C16:0 C18:0 C18:1 C18:2 C18:3

Upon treatment with SA, free palmitic acid (C16:0) accumulation was observed from 48 till 96 hai with an average of 2.38 fold-increase over this period and 1.4-fold increase at the qualitative level over the whole time-course experiment. Similar results were observed for the PLFAs C16:0, essentially the last 3 days of the experiment. Since monomers of cutin are synthesized C16:0, SA seems to induce the reinforcement of the plant cuticule. In *A. thaliana*, levels of the C16:3 (hexadecatrienoic acid) increase within a few hours of exposure to an

Increases in both classes of stearic acid C18:0 content and percentage were observed in SAinfiltrated leaves. In soybean, increased levels of C18:0 likely inhibit soybean seed

A transient 2-fold increase in free FAs C18:1 content was recorded. A sharp and rapid increase in C18:1 level was observed in parsley cells treated with a fungal elicitor [83]. Recent studies suggest that free oleic acid (18:1) levels in the chloroplast regulate the defense

response of plants to pathogens including programmed cell death and SAR [94].

also investigated and are presented in Table 5 and Table 6.

**Table 5.** Variations in free FAs content and % in SA-infiltrated leaves

**Table 6.** Variations in PLFAs content and % in SA-infiltrated leaves

colonization by the seed-borne pathogen *Diaporthe phaseolorum* [93].

2.38-fold increase (48-96hai)

1.4-fold increase (6-96hai)

1.5-fold increase (48-72hai)

1.6-fold increase (48-96hai)

avirulent strain of *P. syringae* [92].

One of the most interesting results is the general decrease of C18:3 level after SA-infiltration. Most of the studies report increases in 18:3 levels such in suspension cells of California poppy (*Eschscholtzia californica*) treated with a yeast elicitor [97]. In *A. thaliana,* an increase of C18:3 occurred within a few hours of exposure to an avirulent strain of *P. syringae* [91]. The *Arabidopsis fad7 fad8* mutant defective in the generation of C18:3 in chloroplastic membranes is deficient in ROS production following infection with avirulent strains of *Pseudomonas syringae* and shows enhanced susceptibility to this pathogen [92]. C18:3 stimulates NADPH oxidase activity *in vitro,* which suggests that C18:3 modulates ROS production and the subsequent defense responses during *R* gene–mediated resistance in plants [92]. The *Arabidopsis fad3 fad7 fad8* triple mutant is unable to accumulate JA because of a deficiency in C18:3 and is highly susceptible to infection by insect larvae [98]. The *fad3 fad7 fad8* mutant plants are also highly susceptible to root rot by *Pythium jasmonium*, and this susceptibility can be alleviated by the exogenous application of MeJA [99]. Rhizobacteria-induced enhanced resistance to *Botrytis cinerea* is associated with the accumulation of C18:2 and C18:3 FAs in bean [100].

In barley leaves, 13-LOX are induced by SA and jasmonates. Upon SA treatment, free C18:3 and C18:2 accumulate in a 10:1 ratio reflecting their relative occurrence in leaf tissues [78]. The release of 18:3 from plant membrane lipids by stress-activated lipases is thought to provide the substrate for lipoxygenase and subsequent octadecanoid (oxylipin) pathway synthesis of JA and methyl jasmonate [101,102]. JA and methyl jasmonate participate in the signal regulation of a number of plant processes including wound and pathogen defense responses. Efforts have been successful to identify and characterize fatty acids esterifying lipases that are activated by pathogen attack and/or environmental stress. Results suggest that both A1 and A2 phospholipases are involved in 18:3 mobilization form membrane lipids [103]. In the C4 monocotyledon sorghum (*Sorghum bicolor L.),* SA induced genes of the octadecanoic acid pathway for JA synthesis which resulted in higher JA content [104].

However, in tobacco tissues expressing a hypersensitive response to TMV, an increase in the saturation of fatty acids contained in the microsomal phospholipids was observed while C18:3 content decreased by 9% [105]. Interestingly, the authors credited the change of FAs composition to a four-fold increase in LOX activity of the infected tobacco tissues.

The decreases in free FAs observed with our model could be explained by a rapid dioxygenation *via* LOX activity. Furthermore, accumulation of C16:0 and C18:0 coupled with no significant increase in C18:1 means that elongation of C16:0 into C18:0 is not followed by desaturation into C18:1, C18:2 and finally, C18:3. Such results could explain the reduced content level of C18:3.

#### **3.6.** *ltp* **gene expression is induced by SA infiltration**

The effect of SA on the expression of a lipid transfer protein-encoding gene *ltp* was also conducted according to the same time-course experiment (figure 8). SA induced a biphasic *ltp* expression pattern: a 1.7-fold increase at 9hai followed by an average of 4.6-fold increase between 48 and 96hai.

Lipids as Markers of Induced Resistance in Wheat: A Biochemical and Molecular Approach 381

traffic of phospholipids and in the transport of cutin monomers. Interestingly, SA induces the expression of the *ltp* gene in the same period when its impact on the lipid metabolism is the most important. One could think that the lipid transfer capacity of these binding proteins participate in the modulation of the lipid scenery upon resistance induction with

The present chapter provides evidences for the effect of resistance inducers on wheat lipid metabolism and presents the strategy we used in order to characterize their mode of action at different levels: total FA content and relative proportion, PA, PE and DAG contents, expression of genes such as PLC and LTP-encoding ones. Lipid metabolism is therefore a marker of induced resistance in wheat. To our knowledge, such findings have never been

Salicylic acid is very likely to induce the formation of PA through the activation of phospholipases C and/or D pathways: induction of *PLC* gene expression, together with DAG accumulation suggests that the PLC pathway is enhanced and leads to PA production. On the other hand, reduction of PE content suggests that PLD pathway is triggered upon SA infiltration in order to ensure PA synthesis. *lox* gene expression up-regulation and corresponding enzymatic activity, along with the decrease of linolenic acid content, suggests that SA modulates lipid enzymatic peroxidation. Moreover, the expression of *ltp* gene was induced by SA, showing the involvement of the corresponding protein in the lipid signaling

The tested resistance inducers had some similarities in their mode of action, relatively to total FAs profiles. Trehalose and Milsana® seem to share similar modes of action via the increase of C12:0 and decrease of C20:2 contents. Iodus® exhibited the largest effects on FAs profiles, inducing increases in C12:0 and C18:1 and decreases in C20:2. HSA, however, was

Future investigations have to be extended to other genes expression and corresponding enzymatic activities acting downstream of lipoxygenase in order to figure out whether the LOX-derived hydroperoxides are metabolized during the JA synthesis. Furthermore, a global approach using microarrays based on wheat cDNA chips would be a useful tool for

increasing our knowledge of the plant lipidome in our wheat-powdery pathosystem.

the only resistance inducer that modulated positively the content of C18:2.

Christine Tayeh, Béatrice Randoux, Frédéric Laruelle,

*Interactions sur le Vivant (UCEIV), France* 

Natacha Bourdon, Delphine Renard-Merlier and Philippe Reignault\* *Université du Littoral Côte d'Opale, Unité de Chimie Environnementale et* 

SA.

**4. Conclusion** 

metabolism.

**Author details** 

Corresponding Author

 \*

presented before on *Triticum aestivum*.

The LTPs extracellular distribution in the exposed surfaces in vascular tissue systems, high abundance and corresponding genes expression in response to infection by pathogens suggest that they are active plant-defense proteins [106]. A combined expression of chitinase and LTP-encoding genes in transgenic carrot plants enhances resistance to *Botrytis sp.* and *Alternaria sp.* [107]. A high global expression of an *ltp* gene in resistant wheat to *Tilletia tritici* was identified [108]. The nonspecific nsLTP-encoding gene expression profile was evaluated in grape cells suspension in response to various defenserealted signal molecules [109]. A rapid and strong accumulation of nsLTPs mRNAs was recorded upon treatment with ergosterol (5h after treatment with hybridation signal more than 300X A.U.) whereas JA, cholesterol and sitosterol promoted an accumulation but to a lesser extent (hybridation signal between 100 and 200X). However, SA had no effect on nsLTPs mRNAs accumulation.

**Figure 8.** *ltp* gene expression in wheat leaves treated with SA

Moreover, LTPs are known to be differentially expressed during a pathogenic interaction because they are potentially good ligands to oleic C18:1, linoleic C18:2 and eicosadienoic acids C20:2 [110]. Among 28 identified wheat nsLTP, eight nsLTP expressed in yeast exhibited lipid binding activity [111]. These proteins could be involved in the intracellular traffic of phospholipids and in the transport of cutin monomers. Interestingly, SA induces the expression of the *ltp* gene in the same period when its impact on the lipid metabolism is the most important. One could think that the lipid transfer capacity of these binding proteins participate in the modulation of the lipid scenery upon resistance induction with SA.
