**9. Perspectives of the CBCh method development**

The wide application in clinical practice of the proposed very sensitive method to penetrate the state of human mitochondria is the most near, reliable and useful perspective. This penetration is even deeper than in the current biochemical studies. The procedure of the measurement is not the main difficulty on this way. It is within the range of the commonly used clinical tests and may be advanced for the convenience.

In order to the CBCh analysis will bring benefit to patients, physicians should acquire deep knowledge of the investigated biochemical processes. Fundamental data from handbooks are not sufficient for this purpose. It is necessary to learn more special area, which is developed in current studies by the CBCh method. This area is advanced even in modern mitochondriology, because it is created during novel experiments. The authors are preparing the special guidance to facilitate the entrance in the new area. We are also glad to help to overcome individual difficulties in the procedure and analysis of data.

Radical approach to the state in the organism through to the preservation of the native structural state of the tissue, found in the study oh DHs, encourages widening the developed conditions on the great variety of the cyto- and histochemical determinations.

Earlier we have stated that the main advantage of the CBCh method lies in the use of lymphocytes as indicators of the overall metabolic status of the entire organism, which is especially useful as an early diagnostic tool of various mitochondrial dysfunctions. This method can be also used for individual selection of drugs in sample of blood of a certain person.

However, the CBCh method may also be used as a tool for the study of lymphocytes as an independent object of study, which carries important functions in the immune response. The use of the CBCh method for the study of dehydrogenase activity, as well as other indicators, is likely to lead to a better understanding of the dependence of the immune response on mitochondrial functions.

The novel area of CBCh investigations of lymphocytes can be related with their use as cells possessing recently discovered pronounced ACH regulation, both - vagus -dependent and, particularly interesting, self-dependent, intracellular, which is based on the internal pool of non-neuronal ACH. The simultaneous study of ACH level and dehydrogenase activity in lymphocytes can provide further penetration into substrate-hormonal regulatory system that was pioneered in our laboratory.
