**7. Detection methods of G6PD deficiency**

Currently, primaquine, which causes hemolysis in G6PD-deficient patients, is the only radical cure of *Plasmodium vivax* infections (Burgoine*, et al.*, 2010). Therefore, screening to detect G6PD deficiency is very important. Various tests can be used for the detection of G6PD deficiency, which are based on the assessment of the NADPH production capacity of G6PD. The most frequently used tests that measure NADPH production are the fluorescent spot test, cytochemical assay and spectrophotometric assay. However, fluorescent spot test and the spectrophotometric assay are not reliable for the detection of heterozygous females. In addition, DNA analysis can be done to detect G6PD deficiency for the homozygous, hemizygous, and heterozygous-deficient patients. However, we have to design primers for all mutations (Peters & Van Noorden, 2009).
