**5. Other CBCh characteristics of mitochondria besides SDH and KDH**

Study of Succinate Dehydrogenase and α-Ketoglutarate Dehydrogenase in Mitochondria Inside Glass-Adhered Lymphocytes Under Physiological Conditions – The Two Dehydrogenases as Counterparts of … 247

in the state of relative physiological quiescence, sick people has no quiescent state in

**Figure 5.** CBCh patterns of mitochondria measured in glass - adhered lymphocytes from intact and stressed rats. Adrenaline administration, 30 min, and PES 24 h. NBT reduction is expressed as a mean

**5.2. KDH activity measurement. Addition of malonate (MAL)** 

Owing to a great number of objects calculated (200-800) the presented data are statistically significant at

The pair of columns in Figure 5 next to SDH presents the KDH activity measurements without and with the addition of MAL. These samples contained also NAD+ as a soluble cofactor of KDH. It is worth reminding that MAL should be obligatory added for correct determination of KDH activity. It was well known but undeservedly forgotten in many polarographic measurements that SUC generated in mitochondria during KGL oxidation may amount to a considerable part of respiration in the presence of KGL added. The SUCdependent portion of respiration should be subtracted from total respiration with KGL. The data presented show that the SUC-dependent portion of KDH activity is absent in the intact state, when SDH is non- active. In contrast, this portion rises in excited animals. It is evident from this example that the own KGL oxidation is negligible under strong excitation. The elimination of the SUC-dependent portion of NBT reduction by MAL allows one to measure KDH activity more precisely. Reciprocal changes in KDH and SDH activities in the presence

The last pairs of columns in Figure 5 presents the data on NBT reduction with the addition of 5 μM, "Signal" concentration of SUC or 5 μM of ADR with substrate concentration of

mitochondria. This will be demonstrated further.

area of formazan granules in 30 lymphocytes

of MAL become also more evident.

**5.3. Signal action of SUC and ADR** 

the level of 0.001 (99.99%)
