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**Chapter 10** 

© 2012 Kondrashova et al., licensee InTech. This is an open access chapter distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

and reproduction in any medium, provided the original work is properly cited.

© 2012 The Author(s). Licensee InTech. This chapter is distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution,

**Study of Succinate Dehydrogenase** 

**and α-Ketoglutarate Dehydrogenase** 

**in Mitochondria Inside Glass-Adhered** 

**Lymphocytes Under Physiological Conditions –** 

In the fields of biochemistry and medicine there is growing interest to the crucial role of mitochondria in changing of physiological states and disease development. This interest is fueled by rapid progress in the modern branches of mitochondriology: Mitochondrial Physiology (MiP) and Mitochondrial Medicine [1-8]. Numerous investigations demonstrated mitochondrial dysfunctions in different experimental models of stress or disease. These results were obtained under clearly pronounced functional disorders induced by strong external stimuli or pathogenic mutations. These observations increased interest in the detection of mitochondrial dysfunctions in the organism as early biomarkers of pathogenic processes. The detection of mitochondrial dysfunction is crucial at subclinical stages of disease because it can lead to more effective prevention and earlier intervention. In spite of this benefit to date the available testing methods cannot properly measure the state of mitochondria in the organism in ex vivo observations. Past successes of respiration measurements in isolated mitochondria distracted attention from obvious, methodological shortcoming of the existing method of mitochondria isolation: intentional destroy of their native structure organization into entire network. It is known nowadays that the responses of mitochondria to various physiological challenges are based on the reversible dissociation

**The Two Dehydrogenases as Counterparts** 

Marie Kondrashova, Marina Zakharchenko, Andrej Zakharchenko,

Natalya Khunderyakova and Eugen Maevsky

Additional information is available at the end of the chapter

http://dx.doi.org/10.5772/50059

**1. Introduction** 

**of Adrenaline and Acetylcholine Regulation** 


**Study of Succinate Dehydrogenase and α-Ketoglutarate Dehydrogenase in Mitochondria Inside Glass-Adhered Lymphocytes Under Physiological Conditions – The Two Dehydrogenases as Counterparts of Adrenaline and Acetylcholine Regulation** 

Marie Kondrashova, Marina Zakharchenko, Andrej Zakharchenko, Natalya Khunderyakova and Eugen Maevsky

Additional information is available at the end of the chapter

http://dx.doi.org/10.5772/50059
