**Author details**

258 Dehydrogenases

**8. Conclusion** 

physiological functions.

under study.

The experience of the reviewed study evidences reliability to approach ex vivo more closely to the state of investigated tissue in the organism. In order to maintain native state of isolated tissue it is necessary to join the both, care of biochemical processes and physical structure. Only combined biochemical and biophysical approaches will provide attaining the desirable goal to study biochemical mechanisms of physiological processes ex vivo.

This approach opens unknown areas in biochemical regulation in the organism, as shown for instance, by SDH and KDH regulation. One of the mostly known phenomenons, OAA inhibition of SDH, is considered as the main regulator of the SDH activity. However, according to investigations under more physiological conditions, it seems to be in many cases rather artifact of "preparative story" of mitochondria. The wide range of SDH states under the physiological and pathological conditions in the organism, compatible with life, are DH hyperactivation, which is far from the inhibition. This state is probably controlled by GTP, produced due to KDH activity in substrate-level phosphorylation during KGL oxidation. This restriction of SDH by KDH is in essence identical to reciprocal interrelationship between parasympathetic a sympathetic nervous regulation of

A very convincing observation of this substrate-hormonal interaction, as judged by the CBCh method, which cannot be achieved in investigation of isolated mitochondria, can be also mentioned as the crucial result of preservation of the native structure of mitochondria

The wide application in clinical practice of the proposed very sensitive method to penetrate the state of human mitochondria is the most near, reliable and useful perspective. This penetration is even deeper than in the current biochemical studies. The procedure of the measurement is not the main difficulty on this way. It is within the range of the commonly

In order to the CBCh analysis will bring benefit to patients, physicians should acquire deep knowledge of the investigated biochemical processes. Fundamental data from handbooks are not sufficient for this purpose. It is necessary to learn more special area, which is developed in current studies by the CBCh method. This area is advanced even in modern mitochondriology, because it is created during novel experiments. The authors are preparing the special guidance to facilitate the entrance in the new area. We are also glad to

Radical approach to the state in the organism through to the preservation of the native structural state of the tissue, found in the study oh DHs, encourages widening the developed conditions on the great variety of the cyto- and histochemical determinations.

help to overcome individual difficulties in the procedure and analysis of data.

**9. Perspectives of the CBCh method development** 

used clinical tests and may be advanced for the convenience.

Marie Kondrashova\* , Marina Zakharchenko, Andrej Zakharchenko, Natalya Khunderyakova and Eugen Maevsky *Institute of Theoretical and Experimental Biophysics Russian Ac. Sci., Pushchino, Russia* 
