**Author details**

Tomáš Gregor *New Technologies - Research centre, University of West Bohemia, Pilsen, Czech Republic* 

Petra Kochová *Department of Mechanics, Faculty of Applied Sciences, University of West Bohemia, Pilsen, Czech Republic* 

Lada Eberlová *Department of Anatomy, Faculty of Medicine in Pilsen, Charles University in Prague, Pilsen, Czech Republic* 

Lukáš Nedorost, Anna Králíčková and Milena Králíčková *Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University in Prague, Pilsen, Czech Republic* 

### Eva Prosecká

190 Injury and Skeletal Biomechanics

**8. Conclusion** 

**Author details** 

Tomáš Gregor

Petra Kochová

*Czech Republic*  Lada Eberlová

*Czech Republic* 

*Prague, Pilsen, Czech Republic* 

**Figure 15.** Estimating the surface in a series of micro-CT sections with arbitrary orientation using an isotropic triple spatial grid of orthogonal lines with a random initial orientation (fakir probe). The test lines of the fakir probe are green. The violet points denote intersections between the test lines and the current section. The left window shows a 3-D view, the right window shows the current section. Only

This chapter reviews the current approaches in micro-CT imaging and the quantitative evaluation of the resulting image data sets. Both automatic image processing methods and interactive stereological methods are applied for the quantification of the volume fraction, surface density, length density, numerical density, orientation and anisotropy. Micro-CT imaging of bone tissue, tissue-engineered bone scaffolds, and microvascular corrosion casts is quantified using unbiased methods that are already acknowledged in quantitative histology.

*New Technologies - Research centre, University of West Bohemia, Pilsen, Czech Republic* 

*Department of Mechanics, Faculty of Applied Sciences, University of West Bohemia, Pilsen,* 

*Department of Anatomy, Faculty of Medicine in Pilsen, Charles University in Prague, Pilsen,* 

*Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University in* 

one third of the triple line system is shown (software Ellipse).

Lukáš Nedorost, Anna Králíčková and Milena Králíčková

*Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, v.v.i., Prague, Czech Republic Institute of Biophysics, Charles University in Prague, Prague, Czech Republic* 

### Václav Liška

*Department of Surgery, Charles University Prague, University Hospital in Pilsen, Pilsen, Czech Republic* 

### Hynek Mírka

*Department of Imaging Methods, Faculty of Medicine in Pilsen, Charles University in Prague, Faculty Hospital in Pilsen, Pilsen, Czech Republic* 

### David Kachlík

*Department of Anatomy, Third Faculty of Medicine, Charles University in Prague, Prague, Czech Republic* 

### Ivan Pirner and Petr Zimmermann

*Department of Cybernetics, Faculty of Applied Sciences, University of West Bohemia, Pilsen, Czech Republic* 

### Zbyněk Tonar

*European Centre of Excellence NTIS - New Technologies for Information Society, Faculty of Applied Sciences, University of West Bohemia in Pilsen, Pilsen, Czech Republic* 
