*2.2.3. Chromosome damage assay*

Cells were seeded into 100 mm dishes and allowed to grow for 48 h and treated as they were in the cytotoxicity assay described above. Cells were harvested and analyzed using published protocols [7]. Briefly, cells were harvested, treated with potassium chloride to swell the cells, fixed and dropped onto slides. Slides were stained with Giemsa, coverslipped and 100 metaphases were analyzed per treatment concentration.
