**7. References**


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418 Chromatography – The Most Versatile Method of Chemical Analysis

considered and compared with chromatographic methods.

Biljana Nigović, Ana Mornar and Miranda Sertić

*University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, Croatia* 

Development of the analytical methods for identification, purity evaluation and quantification of statin drugs has received a great deal of attention in the field of pharmaceutical analysis in recent years. This review includes trends and advances in separation methods developed for the analysis of statin molecules with different physical and chemical properties. The chapter surveys the application of chromatographic techniques for the determination of statins in pharmaceutical dosage forms and biological samples.

Stability indicating methods and papers describing impurity profiling are discussed in this review. Special emphasis is given to sample preparation as unavoidable and delicate step in bioanalitical methods for quantification of statins and their metabolites. The hyphenated technique that incorporates the efficient separation using liquid chromatography and sensitive detection by mass spectrometry has become an indispensable tool for quantification of statins in biological fluids and pharmacokinetic studies. Methods describing simultaneous analysis of different statins as well as drugs in combined pharmaceutical products and other co-administered drugs in therapy of cardiovascular disease are also described. The application of capillary electrophoresis as alternative separation technique for statins is

The use of statin drugs has augmented in recent years and is expected to increase further in the years ahead because high cholesterol and cardiovascular diseases are being diagnosed more frequently. Therefore, the development of new analytical methods for commercially available statins as well as novel upcoming statin drugs will be a future challenging task for

This work was supported through Grant No. 0061117-1240 (Investigation of new methods in analysis of drugs and bioactive substances) from the Ministry of Science, Education and

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**6. Conclusion** 

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**Author details** 

**Acknowledgement** 

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