**Abbreviations**

**1**

**12**

**14**

**III**

**2**

**<sup>3</sup> <sup>4</sup>**

**8**

**13**

**9**

**11**

**II**


Chromatographic data (parameters) can be used for prediction of following feaatures of bile

• Mixed micelles parameters (for example partition coefficient of nitrazepan in bile acids'

Application of retention parameters for prediction of bile acids' properties is convenient for finding the size of the starting set of molecules. If the set is too small (less than ten molecules), by using *in silico* descriptors multiple regression equations are obtained which are overfitted, while by using chromatographic parameters that posses appropriate structural informations about bile acids, simple linear equations are derived with acceptable

*Department of Pharmacy, Faculty of Medicine, University of Novi Sad, Serbia* 

QSA(P)R = Quantitative Structure Activity (Property) Relationship

RPHPLC = Reversed Phase High Pressure (Resolutions) Liquid Chromatography

RPTLC = Reversed Phase Thin Layer Chromarography

PCA = Principal Component Analysis

PC = Principal Component

SWM = Stabilised Water Molecules

NSWM = Non Stabilised Water Molecules

SSMP = steroid skeleton mean plane
