**9. Electrophoresis**

Electrophoresis is a relatively simple and rapid method with high resolution detection of polar compounds like TTX [10, 11, 51]. When 1 μl of TTX (10 MU, corresponding to 2 μg) is applied onto a 5 x 18 cm cellulose acetate membrane (Chemetron, Milano), the ion molecules of TTX move toward the cathode with a mobility (Rm) clearly smaller than that of authentic of STX. The analysis is performed for 30 minutes in an electrolytic buffer solution of 0.08 M Tris-HCl (pH8.7), under the influence of an applied electric field with a constant current of 0.8 mA/cm width. The toxin is visualized in the same manner as described for TLC.
