**Meet the editor**

Leonardo de Azevedo Calderon was born in Brasília, Brazil, in 1976; he obtained his Bachelor (2000) and PhD (2004) degrees in Biological Sciences at the Universidade de Brasília – UnB, Brazil. He was full time professor at the Universidade Federal do Acre – UFAC from 2006 until 2008. Since 2008 he is full time professor of the Medicine College at the Universidade Federal de Ron-

dônia – UNIR and researcher at the Fiocruz Rondônia where he composes the research group of the Centro de Estudos de Biomoléculas Aplicadas a Saúde – CEBio. He has trained MSc and PhD students of the Postgraduate programs in Experimental Biology (UNIR), and Biodiversity and Biotechnology (Rede Bionorte). His research interest focuses on prospection of bioactive molecules from Amazon biodiversity and their physical-chemical and thermodynamic characterization.

Contents

**Preface IX** 

Chapter 1 **Purification of Phospholipases A2** 

**from American Snake Venoms 1** 

Chapter 2 **Use of Chromatography in Animal Ecology 35** 

Chapter 3 **2D-NanoLC-ESI-MS/MS for Separation and** 

Chapter 4 **Analytical Methods for Quantification of** 

Robert Roškar and Tina Trdan Lušin

Chapter 5 **Current Trends in Sample Treatment Techniques for Environmental and Food Analysis 127** 

**for Analyzing Feed Additives 165** 

Chapter 7 **Analysis of Surfactants in Environmental Samples by Chromatographic Techniques 187**  Juan M. Traverso-Soto, Eduardo González-Mazo

Chapter 8 **Application of HPLC Analysis of Medroxyprogesterone** 

and Pablo A. Lara-Martín

**Acetate in Human Plasma 217**  Saksit Chanthai and Thanee Tessiri

Phan Van Chi and Nguyen Tien Dung

Leonardo A. Calderon and Andreimar M. Soares

**Drug Metabolites in Biological Samples 79** 

Chapter 6 **Using High Performance Liquid Chromatography (HPLC)** 

Jolanta Rubaj, Waldemar Korol and Grażyna Bielecka

Rodrigo G. Stábeli, Rodrigo Simões-Silva, Anderson M. Kayano, Gizeli S. Gimenez, Andrea A. Moura, Cleópatra A. S. Caldeira, Antonio Coutinho-Neto, Kayena D. Zaqueo, Juliana P. Zuliani,

Emma B. Casanave, M. Soledad Araujo and Gustavo H. López

**Identification of Mouse Brain Membrane Proteins 63** 

Paolo Lucci, Deborah Pacetti, Oscar Núñez and Natale G. Frega
