**6. Conclusions**

Chromatographic data (parameters) can be used for prediction of following feaatures of bile acids:


Application of retention parameters for prediction of bile acids' properties is convenient for finding the size of the starting set of molecules. If the set is too small (less than ten molecules), by using *in silico* descriptors multiple regression equations are obtained which are overfitted, while by using chromatographic parameters that posses appropriate structural informations about bile acids, simple linear equations are derived with acceptable predictive power.
