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**Chapter 6** 

© 2012 Rubaj et al., licensee InTech. This is an open access chapter distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

© 2012 Rubaj et al., licensee InTech. This is a paper distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

**Using High Performance** 

**Liquid Chromatography (HPLC)** 

Jolanta Rubaj, Waldemar Korol and Grażyna Bielecka

Feed additives have been widely used in animal nutrition. Recommendations concerning using feed additives, their categories and the description of requirements related to such additives can be found in the regulation (EU) No 1831/2003 [1]. Detailed regulations oblige the entities launching feed additives on the market to specify the methods used to analyse active substances of additives for the needs of official feed control. Official feed control is implemented in order to monitor adequate and safe use of feed additives in animal nutrition. Moreover, controlling feed production in this respect results in improving the

HPLC methods have been widely used in the analyses of feed additives, such as vitamins, feed colorants, antioxidants, amino acids and coccidiostats in preparations, premixes and feed mixtures. It is relatively simple to analyse preparations as they are usually composed of a particular active substance and a carrier. A premix is more complex feed consisting of a combination of a dozen different feed additives on a mineral (calcium carbonate) or organic (wheat bran) carrier. Complete feed mixtures used in animal nutrition, produced by combining premixes with feed materials are often greased and subjected to further hydro- or barothermal processing, e.g. pelleting, extrusion or expanding. In order to counteract decomposition of the active substance, feed additives are secured by protective coating, e.g. vitamin A, canthaxanthin, which enhances their durability in the feed matrix. A specific protection of feed additives by protective coating, thermal processing, greasing the feed, varied composition of feed materials in mixtures may hinder the transfer of the active

The key issue becomes selection of a chromatographic column (in a normal phase or reversed phase), mobile phase, detector, as well as optimization the conditions of

substance into a solution during extraction and purification of the extract.

**for Analyzing Feed Additives** 

Additional information is available at the end of the chapter

quality and safety of animal products for consumers.

http://dx.doi.org/10.5772/47784

**1. Introduction** 

