**4.2. Subsequent purification of AMY or GLL1 in chromatography columns**

Intended for their characterization, AMY and GLL1 were independently collected and subjected to subsequent purification with DEAE-Toyopearl 650M AEX column. An SDS PAGE analysis showed that these purification steps resulted in pure AMY (5) and GLL1 (16). The final purification scheme of AMY is summarized in Table 1. Further, whilst the presence of two and three types of α-amylase and glucoamylase, respectively, were reported upon purification of these amylolytic enzymes from strain KZ by a Mono-Q anion exchanger column (22), AMY and GLL1 appeared to be the only amylolytic enzyme species from strain R64. Nevertheless, additional analysis was performed to confirm that the isolation of purified AMY and GLL1 was unanimous.


**Table 1.** The final purification scheme of AMY. AS stands for ammonium sulfate.
