**Abbreviations**

AEX, anion exchange chromatography; AMY, α-amylase from *S. fibuligera* R64; CD, cyclodextrin; CEX, cation exchange chromatography; DEAE, diethyl amino ethyl; DNA, deoxyribose nucleic acid; EDTA, ethylene diamine tetra acetate; FPLC, fast protein liquid chromatography; GLA, glucoamylase from *S. fibuligera* KZ; GLL1, glucoamylase from *S. fibuligera* R64; GLU, glucoamylase from *S. fibuligera* HUT7212; HCA, hydrophobic cluster analysis; HIC, hydrophobic interaction chromatography; LC, liquid chromatography; PDB, Protein Data Bank; PGK, phosphoglycerate kinase; RP-HPLC, reversed-phase high performance liquid chromatography; SDS PAGE, sodium dodecyl-sulfate polyacrylamide gel electrophoresis; SEC, size-exclusion chromatography; TFA, trifluoroacetic acid; TLC, thin layer chromatography; TPCK, tosyl phenylalanyl chloromethyl ketone.
