**1. Introduction**

270 Chromatography – The Most Versatile Method of Chemical Analysis

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Chromatography, at both preparative and analytical levels, has been a key experimental technique in the study of proteins, primarily liquid chromatography in the separation, purification, and analysis. Recent developments in the study of proteins lean towards simplification and miniaturization, thus chromatography becomes less involved and explored. For example, development of protein tags and their associated affinity matrices enables purification of a protein in one step. This chapter describes the identification and structure-function relation study of amylolytic enzymes from *Saccharomycopsis fibuligera* R64, where liquid chromatography was used as the major tool, not only in the purification but also biochemical analysis of the enzymes.
