**3.2 Multiplex PCR gene detection**

The multiplex PCR assay detected the 100% *bla*TEM genes, 25% *bla* SHV and 52% *bla* CTX-M genes among the *E. coli* isolates. Similarly, 94% *bla*TEM, 42% *bla*SHV and 70% *bla*CTX-M genes in the *K. pneumoniae* isolates were detected. All CTX-M genes were identified as alleles belonging to the phylogenetic group I.
