Contents

#### **Preface XIII**


	- **Part 2 Electrophoresis Application in Bacteriology, Parasitology, Mycology and Public Health 111**

Contents VII

**Part 5 Temporal Temperature Gel Electrophoresis 289** 

**to Survey Pathogenic Bacterial Communities: The Case of Surgical Site Infections 291**  Romano-Bertrand Sara, Parer Sylvie, Lotthé Anne, Colson Pascal, Albat Bernard and Jumas-Bilak Estelle

**Part 6 Two-Dimensional Gel Electrophoresis (2-DE) 313** 

**and Mass Spectrometry in Studies of Nanoparticle-Protein Interactions 327**  Helen Karlsson, Stefan Ljunggren, Maria Ahrén, Bijar Ghafouri, Kajsa Uvdal, Mats Lindahl

**Electrophoresis in Cancer Proteomics 359**  Soundarapandian Kannan, Mohanan V. Sujitha,

**Part 7 Other Applications of Gel Electrophoresis Technique 391**

**Degradation by** *Lentinula edodes* **on Native-PAGE 393**

**Phenol-Oxidizing Isozymes Involved in Lignin-**

Eiji Tanesaka, Naomi Saeki, Akinori Kochi and

Chapter 21 **Protection Studies by Antioxidants Using Single Cell Gel** 

**Polymorphism and Nutritive Value of the** 

**of the Radiolabeled Monoclonal Antibodies 447** 

**Seed Storage Proteins in the Grain Sorghum 463** Lev Elkonin, Julia Italianskaya and Irina Fadeeva

**Electrophoresis (Comet Assay) 413**

Chapter 22 **Gel Electrophoresis as Quality Control Method** 

Shenbagamoorthy Sundarraj and Ramasamy Thirumurugan

Chapter 20 **Enzymatic Staining for Detection of** 

Motonobu Yoshida

Pınar Erkekoglu

Veronika Kocurová

Chapter 23 **Gel Electrophoresis as a Tool to Study** 

Chapter 17 **Two-Dimensional Gel Electrophoresis Reveals Differential** 

**Protein Expression Between Individual** *Daphnia* **315**  Darren J. Bauer, Gary B. Smejkal and W. Kelley Thomas

Chapter 16 **Temporal Temperature Gel Electrophoresis**

Chapter 18 **Two-Dimensional Gel Electrophoresis**

and Anders Ljungman

Chapter 19 **Two Dimensional Gel** 

	- **Part 3 Electrophoresis Application in the Analysis of Protein-Nucleic Acid Interaction and Chromosomal Replication 203**
	- **Part 4 Electrophoresis Application in Enzymology 245**

#### **Part 5 Temporal Temperature Gel Electrophoresis 289**

Chapter 16 **Temporal Temperature Gel Electrophoresis to Survey Pathogenic Bacterial Communities: The Case of Surgical Site Infections 291**  Romano-Bertrand Sara, Parer Sylvie, Lotthé Anne, Colson Pascal, Albat Bernard and Jumas-Bilak Estelle

VI Contents

Chapter 8 **Molecular Microbiology Applied to** 

Jesús M. Cantoral

**the Study of Phytopathogenic Fungi 139** 

Victoria E. González-Rodríguez, Eva Liñeiro and

Chapter 9 **Molecular and Proteolytic Profiles of** *Trypanosoma cruzi* **Sylvatic Isolates from Rio de Janeiro-Brazil 157** Suzete A. O. Gomes, Danielle Misael, Cristina S. Silva, Denise Feder, Alice H. Ricardo-Silva, André L. S. Santos, Jacenir R. Santos-Mallet and Teresa Cristina M. Gonçalves

Chapter 10 **Pulsed Field Gel Electrophoresis in Molecular** 

Chapter 11 **Usefulness of Pulsed Field Gel Electrophoresis** 

**Part 3 Electrophoresis Application in the** 

Chapter 12 **Electrophoretic Mobility Shift Assay:** 

Chapter 13 **Analysis of Chromosomal Replication** 

Chapter 14 **Polyacrylamide Gel Electrophoresis an**

Carolina Alves and Celso Cunha

Carlos Garrido, Francisco J. Fernández-Acero, María Carbú,

**Typing and Epidemiological Detection of Methicillin Resistant** *Staphylococcus aureus* **(MRSA) 179** Velazquez-Meza Maria Elena, Vázquez-Larios Rosario, Hernández Dueñas Ana Maria and Rivera Martínez Eduardo

**Assay in the Molecular Epidemiological Study of Extended Spectrum Beta Lactamase Producers 193** 

Patrick Eberechi Akpaka and Padman Jayaratne

**Analysis of Protein-Nucleic Acid Interaction** 

**Progression by Gel Electrophoresis 229**  Elena C. Guzmán and Enrique Viguera

**Part 4 Electrophoresis Application in Enzymology 245**

**Important Tool for the Detection and Analysis**

Carlos A. Leal-Morales, Alberto Flores Martínez, Patricia Ponce Noyola and Roberto Zazueta-Sandoval

Chapter 15 **Applications of Zymography (Substrate-SDS-PAGE)** 

José Batista de Jesus and Marta H. Branquinha

Reyna Lucero Camacho Morales, Vanesa Zazueta-Novoa,

**for Peptidase Screening in a Post-Genomic Era 265** Claudia M. d'Avila-Levy, André L. S. Santos, Patrícia Cuervo,

**of Enzymatic Activities by Electrophoretic Zymograms 247** 

**Analyzing Protein – Nucleic Acid Interactions 205** 

**and Chromosomal Replication 203** 

#### **Part 6 Two-Dimensional Gel Electrophoresis (2-DE) 313**

	- **Part 7 Other Applications of Gel Electrophoresis Technique 391**

XII Contents

#### Chapter 24 **Extraction and Electrophoresis of DNA from the Remains of Mexican Ancient Populations 479**  Maria de Lourdes Muñoz, Mauro Lopez-Armenta, Miguel Moreno-Galeana, Alvaro Díaz-Badillo, Gerardo Pérez-Ramirez, Alma Herrera-Salazar, Elizabeth Mejia-Pérez-Campos, Sergio Gómez-Chávez and Adrián Martínez-Meza
