**4. Concluding remarks**

Replication arrest is a source of genetic instability in all types of living cells. As a consequence, cells have developed several effective strategies to tackle with replication fork arrest and/or repairing the double DSBs generated at the stalled replication forks. We have reviewed how PFGE and 2D gels can be used to elucidate some features related to the progression of the replication forks. By using these two non-conventional electrophoresis it can be verified the presence of stalled replication forks, understanding how they have been generated and how they could be restarted.
