**2. Why comet assay is a suitable tool for antioxidant research?**

Comet assay can easily detect the *in vitro* toxicity of environmental chemicals on different cell types, as well as *in vivo* toxicity in tissue samples obtained from animals. Besides, it is also a valid technique to evaluate whether antioxidants/micronutrients are able to protect the integrity of the genetic material **(Anderson et al., 1997; Heaton et al., 2002; Novotna et al., 2007)**.

The benefits of Comet assay can be summarized as below:


The advantages and disadvantages of Comet assay are shown in **Figure 1**.
