**2.2 Harvesting of** *Daphnia*

*Daphnia* gut contents were minimized by allowing the microcrustaceans to feed on copolymer microspheres of 4.3-micron mean diameter (Duke Scientific, Fremont, CA, USA) for one hour prior to harvesting. Microspheres were fed at a concentration equal to the number of algal cells previously supplied. *Daphnia* were harvested by filtration through 250 um Nitex mesh (Sefar America, Depew, NY, USA) and flash frozen. Average mass of adult *Daphnia pulex* was 0.1158 + 8.3 mg fully hydrated and 0.05285 + 10.60 mg dehydrated (*n* = 50). Average mass of adult *D. magna* was 1.37+ 0.46 mg fully hydrated and 0.23 + 0.06 mg when dehydrated (*n* = 64).
