**4. Conclusion**

Characteristics of the NIRS method as being applied for protein content determination tested within the validation experiment expressed superior quality over the wet chemical method for protein content determinaton. Due to that, it is not surprising that the NIRS method has been accepted as the standard method by the ISO, AACC, AOAC and ICC, which confirms its applicability for routine use. Before its adoption as an official analytical method, special attention had to be paid to the regulatory requirements for analytical procedures especially due to the fact that the NIRS technique heavily relies on the use of chemometrics calibration and statistical analysis of data. For this reason, it is of upmost importance to verify the characteristics of the NIRS method to demonstrate its fitness for intended purpose. The validation protocol for the NIRS method presented represents an important contribution to the accreditation of the NIRS method based on the requirements of ISO/IEC 17025.
