**5. Sample collection, results and discussion**

Due to unavailability of spectrofluorometer system that can be directly linked with the setup, sampled data collected from fluorescence decay experiment conducted using Spectramax Germini XS system were used to test the performance of the integrated system. The schematic diagram of the spectrofluorometer operation is shown in Figure 9, and itemized as follows:


Three intrinsic fluorophores (Acridine Orange; Fluorescein Sodium and Quinine) were used in the experiment. The details of the substances are given in Table 1.


**Table 1.** Characteristics of the fluorophores samples

The simulation results for Acridine orange, Fluorescein Sodium, Quinine, Quinine plus Arcridine, Fluorescein Sodium plus Acridine orange, and Fluorescein Sodium plus Acridine orange plus Quinin in water are shown in Figure 10 (a-f) respectively.

Figure 11 to Figure 13 show the sample of results obtained from the integrated MATLAB-Labview real-time software which has been developed. Both results of simulation and realtime software interface yield accurate estimates of the fluorescence data as shown in Figure 10- Figure 13, and presented in Table 2. The singular values for each of the samples combination are given in Table 3. The results indicate accurate determination of the constituent samples

**Figure 9.** Schematic diagram of the SPECTRAMAX Gemini Spectrofluorometer operation (SPECTRAmax®)

C17H12ClN3 Merck 301.8g/mol 99.1% Solid Orange red 28g/l Soluble

orange plus Quinin in water are shown in Figure 10 (a-f) respectively.

**Table 1.** Characteristics of the fluorophores samples

Molecular formula Manufacturer Molecular weight Purity by HPLC

Solubility in water Solutility in ethanol

Form Colour Acridine Orange Fluorescein Sodium Quinine

C20H10Na2O5 Merck 376.28 Extra pure Solid

C20H24N2O2 Merck 324.43 Extra pure Powder, White 0.5g/l 1200g/l

Reddish brown

500g/l 140g/l

The simulation results for Acridine orange, Fluorescein Sodium, Quinine, Quinine plus Arcridine, Fluorescein Sodium plus Acridine orange, and Fluorescein Sodium plus Acridine

Figure 11 to Figure 13 show the sample of results obtained from the integrated MATLAB-Labview real-time software which has been developed. Both results of simulation and realtime software interface yield accurate estimates of the fluorescence data as shown in Figure 10- Figure 13, and presented in Table 2. The singular values for each of the samples combination are given in Table 3. The results indicate accurate determination of the constituent samples

**Figure 9.** Schematic diagram of the SPECTRAMAX Gemini Spectrofluorometer operation (SPECTRAmax®)

**Figure 10.** Sample simulation results with embedded MATLAB function
