Fig. 2.

The ethidium bromide-stained 2.5% agarose gel shows the PCR products of 9 blood samples, some of which are SV40 positive. N10(-) control (water), 11 (+) control (DNA extracted from blood of SV40+ positive M. mulatta).

The obtained samples were sequenced on a ABI Prism 377 sequenator. Result of sequencing confirmed belonging of amplified regions to SV40.

We believe that the results can be corrected both towards the increase and towards reduction on the basis of age, sex and other factor analyzes of representative groups.

Nevertheless obtained material allows drawing some conclusions. High percent of SV40 infected children in Sochi and Krasnodar region at the age of 10 years who were immunized with poliovaccine reliably free from contamination with this virus definitely testifies horizontal way of contamination.

Numerous publications are devoted to polyomavirus problem and a great part of them to SV40 problem: reliability or unauthenticity of SV40 detection in healthy people, in human neoplasm, SV40 role in tumor emergence, SV40 origin and ways of spreading are discussed. In a great degree a lot of publications are connected with scandalous character of the problem – SV40 introduction into human population during immunization against poliomyelitis.

Despite the fact that preparing vaccines on rhesus kidney cell cultures was stopped about 30 years ago SV40 carrying is still determined in high percent of cases in healthy persons and also in some malignant human neoplasm (brain tumors, mesotheliomas, osteosarcomas etc.).

Polyomavirus SV40 has been detected practically on all continents (Fig. 2). After introduction it into human population it should be considered as human virus – man has become its reservoir. The virus circulates among people, spreading horizontally, excretes into surrounding, pollutes it and this is perhaps a very important factor of its epidemiology.

Fig. 3. SV40 infection of population was discovered on all Continents. Black color designates countries where SV40 carriers were discovered. However it does not mean the prevalence of SV40 infection.
