**4. Сonclusion**

262 Epidemiology Insights

Because this investigation revealed a rather high number of SV40 carriers in Krasnodar

To confirm the results of RQ PCR, and prepare amplicons for sequencing another sets (SV5/SV6) of primers directed to conservative region 173-bp length of SV40 T-ag were used. These PCR primers amplify a region of SV40 that could be distinguished from BK and JC

The ethidium bromide-stained 2.5% agarose gel shows the PCR products of 9 blood samples, some of which are SV40 positive. N10(-) control (water), 11 (+) control (DNA

The obtained samples were sequenced on a ABI Prism 377 sequenator. Result of sequencing

We believe that the results can be corrected both towards the increase and towards

Nevertheless obtained material allows drawing some conclusions. High percent of SV40 infected children in Sochi and Krasnodar region at the age of 10 years who were immunized with poliovaccine reliably free from contamination with this virus definitely testifies

reduction on the basis of age, sex and other factor analyzes of representative groups.

Sochi 102 44 43 Adler 150 48 32

Number of SV40 positive

number %

Number of SV40 positive Absolute number %

49 16 14 29.6 26.7

Absolute

samples

region we decided to check the situation in Sochi and Adler (Table 3).

samples

extracted from blood of SV40+ positive M. mulatta).

confirmed belonging of amplified regions to SV40.

horizontal way of contamination.

Region Number of

Krasnodar region

Region Number of

viral DNA. (Testa *et al.* 1998).

Moscow St. Petersburg Novosibirsk Krasnoyarsk region

Table 2.

Table 3.

Fig. 2.

In this article data on analysis of SV40 polyomavirus contamination of population in some regions of Russia are presented. Infection was detected by PCR in real time (RQ-PCR) with TaqMan probe directed to the site of 9 nucleotide deletion distinguishing SV40 from ubiquitous human viruses JCV and BKV, The source of infection was mainly vaccination

Epidemiology of Simian Polyomavirus SV40 in Different Areas of Russian Federation (RF) 265

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with Sabin OPV vaccine contaminated with SV40. Virus SV40 was detected in archive vaccine samples produced in East European countries before 1978 because heating with MgCl2 did not free the vaccine from SV40 contamination. Infection rate was investigated in 5 Russian regions: Russia (Moscow, St. Petersburg, cities of the Black Sea coast, Novosibirsk and Krasnoyarsk region).

The highest values were detected on the Black Sea and the lowest in St. Petersburg. Presence of SV40 in children vaccinated with reliably decontaminated vaccine confirms possibility of horizontal spreading of the virus. According to reference data contamination of population was revealed on all Continents.

Despite the fact that preparing polio vaccine on culture of M.mulatta kidney cells was stopped 30 years ago SV40 carriage still exists in healthy people in rather high percent and also in human malignant neoplasms practically in all countries, on all Continents (Basetse, 2002; Leithner et al., 2006; Minor et al., 2003; Zekri et al., 2007; Dang-Tan et al., 2004). Polyomavirus SV40 was introduced into human population and should be considered as human virus. Man has become its reservoir. The virus circulates among people, spreads horizontally, excretes into environment and pollutes it and this is an important factor in its epidemiology.

Perhaps true value of SV40 prevalence in different regions can be established at examination of randomized groups generated on age, sex, place of residing, conditions of life and cultural skills.
