**3. Results**

In total 768 blood samples obtained from different cities and regions of Russian Federation (St. Petersburg - 56, Moscow – 50, Krasnodar region – 352, Novosibirsk – 108, Krasnoyarsk region – 202) have been investigated by RQ-PCR. Results of these investigations are presented in Table 2.

Epidemiology of Simian Polyomavirus SV40 in Different Areas of Russian Federation (RF) 263

Numerous publications are devoted to polyomavirus problem and a great part of them to SV40 problem: reliability or unauthenticity of SV40 detection in healthy people, in human neoplasm, SV40 role in tumor emergence, SV40 origin and ways of spreading are discussed. In a great degree a lot of publications are connected with scandalous character of the problem – SV40 introduction into human population during immunization against

Despite the fact that preparing vaccines on rhesus kidney cell cultures was stopped about 30 years ago SV40 carrying is still determined in high percent of cases in healthy persons and also in some malignant human neoplasm (brain tumors, mesotheliomas,

Polyomavirus SV40 has been detected practically on all continents (Fig. 2). After introduction it into human population it should be considered as human virus – man has become its reservoir. The virus circulates among people, spreading horizontally, excretes into surrounding, pollutes it and this is perhaps a very important factor of its

Fig. 3. SV40 infection of population was discovered on all Continents. Black color designates countries where SV40 carriers were discovered. However it does not mean the prevalence of

In this article data on analysis of SV40 polyomavirus contamination of population in some regions of Russia are presented. Infection was detected by PCR in real time (RQ-PCR) with TaqMan probe directed to the site of 9 nucleotide deletion distinguishing SV40 from ubiquitous human viruses JCV and BKV, The source of infection was mainly vaccination

poliomyelitis.

osteosarcomas etc.).

epidemiology.

SV40 infection.

**4. Сonclusion**


Table 2.

Because this investigation revealed a rather high number of SV40 carriers in Krasnodar region we decided to check the situation in Sochi and Adler (Table 3).


Table 3.

To confirm the results of RQ PCR, and prepare amplicons for sequencing another sets (SV5/SV6) of primers directed to conservative region 173-bp length of SV40 T-ag were used. These PCR primers amplify a region of SV40 that could be distinguished from BK and JC viral DNA. (Testa *et al.* 1998).
